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The map label for this gene is metB [H]

Identifier: 18977638

GI number: 18977638

Start: 1198668

End: 1199768

Strand: Direct

Name: metB [H]

Synonym: PF1266

Alternate gene names: 18977638

Gene position: 1198668-1199768 (Clockwise)

Preceding gene: 18977636

Following gene: 18977639

Centisome position: 62.81

GC content: 40.51

Gene sequence:

>1101_bases
ATGGAGCCTCTTCATTATCCTATCTATTTGACTGCAGTATTCAAGCAGATAGGGGATGCCTATCTTACTGAAAGAGGTAC
AGAGCTAAAGTATTCAAGGGAAGAAAATCCAACAGTAAGAAATTTGGAAGATAAGCTTGCTAAGCTAGAGAATGCTGAGA
ATGCCCTGGCATTTAACAGTGGAATGGGGGCTATCTCGACTTTGTTCCTATCCTTGCTTAAAGCTGGAGACGAAATTGTT
ATGTCAATGGAGGGGTATGGAACCACAATTGAGGTACCAAAGCTTTTAGAAAAATTTGGAGTTAGAGTAAGGCTTGCCTA
TCCCTCAGCAGAAAAAATTTGTGAAACTATAACGTCCAATACAACTCTTGTTTTCATTGAAACAATGACTAACCCAACTT
TAAAGGTAATCGACGTTAGGGAAGTTGCTAAAAAATGCAGAGAGGAAGAAATCACTTTAATAGTTGACAACACATTTGTT
ACCCCCATCCTCTACAGGCCCCTTGAAGATGGAGTTAATTTCGTCGTTCATAGCTTGACAAAGTACATTGCGGGACACAA
CGATGTTACTGGTGGTGTAATTCTTTGGAATGGAAAATTCTTAAATGATTTATGGGACTGGAGGAGAAGATTGGGAACGA
TAATCCAACCATTTGACGCCTGGATGGTTGAAAGAGGCATGAGAACCCTAAATGTTAGGTTTGAAAAGCAGAGTAAAAAT
GCCTTAGCAATAGCAGAATTCCTGCAGGATCATCCAAAGGTTAGGAAAGTACACTACCCAGGACTACCTAGTGATCCTTA
TCATGGCATAGCTAGGAGATTATTTAGGAAGGATCTTTATGGGGGAGTAGTTTCTTTTGACCTGGGAAGTGAAAAAAGCG
CACTTGCATTCCTTAGAAGCCTAAAGAAAATATTTCCCTCTCCCTCTCTTGGAGGTGTGGAAAGTCTTGCAACTTATCCT
GTAAAAAGCGCCGTAAAGAACATGGAAAAAGAAACTAGAGACCTTCTTGGAATAACGGATGGTCTAATTAGGCTTTCTGT
GGGAATAGAAGAGGTTGATGAGCTAATAGAGGACTTAGATAGGGCCCTTAAGGGGTGTTAG

Upstream 100 bases:

>100_bases
TTACTTTTGCAAGTTTAATTCTTTGTGGTTATATTTATGTGTCATTTTTATGTCGAAAAATATATAAAGTAAATTTAGAA
GTCTTGGTAGAGGTGAGAGA

Downstream 100 bases:

>100_bases
GTGTGAGGGTGTTTGGTTGTCCAAAGGATCTTCTAAACGGTCCCTGTGGAGGAGCACTCGATGGAAAGTGTGAGGTAAAT
TCAAATCTCTGTCCATGGTA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MEPLHYPIYLTAVFKQIGDAYLTERGTELKYSREENPTVRNLEDKLAKLENAENALAFNSGMGAISTLFLSLLKAGDEIV
MSMEGYGTTIEVPKLLEKFGVRVRLAYPSAEKICETITSNTTLVFIETMTNPTLKVIDVREVAKKCREEEITLIVDNTFV
TPILYRPLEDGVNFVVHSLTKYIAGHNDVTGGVILWNGKFLNDLWDWRRRLGTIIQPFDAWMVERGMRTLNVRFEKQSKN
ALAIAEFLQDHPKVRKVHYPGLPSDPYHGIARRLFRKDLYGGVVSFDLGSEKSALAFLRSLKKIFPSPSLGGVESLATYP
VKSAVKNMEKETRDLLGITDGLIRLSVGIEEVDELIEDLDRALKGC

Sequences:

>Translated_366_residues
MEPLHYPIYLTAVFKQIGDAYLTERGTELKYSREENPTVRNLEDKLAKLENAENALAFNSGMGAISTLFLSLLKAGDEIV
MSMEGYGTTIEVPKLLEKFGVRVRLAYPSAEKICETITSNTTLVFIETMTNPTLKVIDVREVAKKCREEEITLIVDNTFV
TPILYRPLEDGVNFVVHSLTKYIAGHNDVTGGVILWNGKFLNDLWDWRRRLGTIIQPFDAWMVERGMRTLNVRFEKQSKN
ALAIAEFLQDHPKVRKVHYPGLPSDPYHGIARRLFRKDLYGGVVSFDLGSEKSALAFLRSLKKIFPSPSLGGVESLATYP
VKSAVKNMEKETRDLLGITDGLIRLSVGIEEVDELIEDLDRALKGC
>Mature_366_residues
MEPLHYPIYLTAVFKQIGDAYLTERGTELKYSREENPTVRNLEDKLAKLENAENALAFNSGMGAISTLFLSLLKAGDEIV
MSMEGYGTTIEVPKLLEKFGVRVRLAYPSAEKICETITSNTTLVFIETMTNPTLKVIDVREVAKKCREEEITLIVDNTFV
TPILYRPLEDGVNFVVHSLTKYIAGHNDVTGGVILWNGKFLNDLWDWRRRLGTIIQPFDAWMVERGMRTLNVRFEKQSKN
ALAIAEFLQDHPKVRKVHYPGLPSDPYHGIARRLFRKDLYGGVVSFDLGSEKSALAFLRSLKKIFPSPSLGGVESLATYP
VKSAVKNMEKETRDLLGITDGLIRLSVGIEEVDELIEDLDRALKGC

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=42.032967032967, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=38.0165289256198, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI25453487, Length=362, Percent_Identity=35.9116022099448, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1790375, Length=362, Percent_Identity=34.8066298342541, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1789383, Length=341, Percent_Identity=29.3255131964809, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560056, Length=365, Percent_Identity=40.2739726027397, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17538055, Length=369, Percent_Identity=39.8373983739837, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI71980708, Length=377, Percent_Identity=33.9522546419098, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319307, Length=365, Percent_Identity=39.4520547945205, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321254, Length=384, Percent_Identity=30.2083333333333, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323333, Length=382, Percent_Identity=28.0104712041885, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=266, Percent_Identity=31.203007518797, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=341, Percent_Identity=26.6862170087977, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322970, Length=266, Percent_Identity=27.0676691729323, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922554, Length=364, Percent_Identity=42.5824175824176, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41204; Mature: 41204

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLHYPIYLTAVFKQIGDAYLTERGTELKYSREENPTVRNLEDKLAKLENAENALAFNS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEEECC
GMGAISTLFLSLLKAGDEIVMSMEGYGTTIEVPKLLEKFGVRVRLAYPSAEKICETITSN
CCHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCCEEEEECCCHHHHHHHHCCC
TTLVFIETMTNPTLKVIDVREVAKKCREEEITLIVDNTFVTPILYRPLEDGVNFVVHSLT
CEEEEEEECCCCCEEEEEHHHHHHHHCCCCEEEEECCCEECHHHHCCHHHHHHHHHHHHH
KYIAGHNDVTGGVILWNGKFLNDLWDWRRRLGTIIQPFDAWMVERGMRTLNVRFEKQSKN
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
ALAIAEFLQDHPKVRKVHYPGLPSDPYHGIARRLFRKDLYGGVVSFDLGSEKSALAFLRS
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHH
LKKIFPSPSLGGVESLATYPVKSAVKNMEKETRDLLGITDGLIRLSVGIEEVDELIEDLD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RALKGC
HHHCCC
>Mature Secondary Structure
MEPLHYPIYLTAVFKQIGDAYLTERGTELKYSREENPTVRNLEDKLAKLENAENALAFNS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEEECC
GMGAISTLFLSLLKAGDEIVMSMEGYGTTIEVPKLLEKFGVRVRLAYPSAEKICETITSN
CCHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCCEEEEECCCHHHHHHHHCCC
TTLVFIETMTNPTLKVIDVREVAKKCREEEITLIVDNTFVTPILYRPLEDGVNFVVHSLT
CEEEEEEECCCCCEEEEEHHHHHHHHCCCCEEEEECCCEECHHHHCCHHHHHHHHHHHHH
KYIAGHNDVTGGVILWNGKFLNDLWDWRRRLGTIIQPFDAWMVERGMRTLNVRFEKQSKN
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
ALAIAEFLQDHPKVRKVHYPGLPSDPYHGIARRLFRKDLYGGVVSFDLGSEKSALAFLRS
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHH
LKKIFPSPSLGGVESLATYPVKSAVKNMEKETRDLLGITDGLIRLSVGIEEVDELIEDLD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RALKGC
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]