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The map label for this gene is rsmF [H]

Identifier: 18977629

GI number: 18977629

Start: 1191037

End: 1191969

Strand: Reverse

Name: rsmF [H]

Synonym: PF1257

Alternate gene names: 18977629

Gene position: 1191969-1191037 (Counterclockwise)

Preceding gene: 18977630

Following gene: 18977625

Centisome position: 62.46

GC content: 40.51

Gene sequence:

>933_bases
ATGTTGGAGAAACTTTTTTCCCTCGGTTATTCTAAAACTTTTGCAGAAAGATACTATCAACTTTGGGGTGAAAGAGCTAT
AAGAATTGCTGAGGCCATGGAAAAACCCTTGCCCAGGTGTTTTAGAGTCAATACGCTTAAAGGAACTATCTCAGAGATAA
CTAAGAGATTAATGAAGAAGGGGTTCCAATTCACCAGAGTCCCCTGGGCAAGAGAAGGATTTTGCTTAACTCGTGAACCA
TTTTCAATAACATCCACGCCTGAATATCTAACAGGCCTCATCTATATTCAAGAGGCAAGCTCAATGTACCCCCCAGTTGC
CCTAGATCCAAAACCAGGGGAGATAGTTGCAGACATGGCCGCGGCCCCTGGGGGAAAAACCAGTTATATGGCCCAGTTAA
TGGAAAATAAAGGATTAATCTTTGCCTTTGATGTAGATGAGGAAAGGTTAAAGGAAACGAGGCTTAACCTCTCCAGGTTA
GGGGTTTTAAACACAATTCTTATTCACAAGTCATCCTTGTATATAGATGAGCTAAATATGAGCTTTGATAAAATCCTCCT
CGATGCTCCATGTACCGGTTCTGGAACAATTCACAAAAATCCTGAGAGAAAGCACAATAGAACGTTAGATGATATAAAAT
TCTGTCAAGGGCTTCAGATGAAGATGATAGAGAAAGCCCTTGAAGTTCTGAAACCCGGAGGAATTTTAGTCTATTCAACA
TGCTCGTTAGAACCAGAGGAGAATGAATTCGTAATTCAATGGGCCCTAGATAACTTTGATATTGAACTCCTTCCCCTGAG
GTATGGAGAACCTGCCTTGATTAATCCCTTTGGCATCGAGCTCAGTCCAGAAATAAAAAAGGCAAGAAGATTTTATCCTG
ACACTCACAAAACAAGTGGATTTTTCATAGCTAAAATTAGAAAGAAAGGCTAG

Upstream 100 bases:

>100_bases
AACACTATCCCAGGAGTAGTTGAAAATGGAATTTTTGCCGATATAGCGGACATAGTATTAGTAGGGACAAAAGAAGGAGT
AAAAAGATTAGAAAGGTAAA

Downstream 100 bases:

>100_bases
AGAAATGCATGCCTATGTTTTGTAAGTCCATACAATCCATAAGCTAACAAGGCAAAGTCCACTATAGAGAGTATTATTAC
AACATAGGCATAAACAACTA

Product: nol1-nop2-sun family nucleolar protein III

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLEKLFSLGYSKTFAERYYQLWGERAIRIAEAMEKPLPRCFRVNTLKGTISEITKRLMKKGFQFTRVPWAREGFCLTREP
FSITSTPEYLTGLIYIQEASSMYPPVALDPKPGEIVADMAAAPGGKTSYMAQLMENKGLIFAFDVDEERLKETRLNLSRL
GVLNTILIHKSSLYIDELNMSFDKILLDAPCTGSGTIHKNPERKHNRTLDDIKFCQGLQMKMIEKALEVLKPGGILVYST
CSLEPEENEFVIQWALDNFDIELLPLRYGEPALINPFGIELSPEIKKARRFYPDTHKTSGFFIAKIRKKG

Sequences:

>Translated_310_residues
MLEKLFSLGYSKTFAERYYQLWGERAIRIAEAMEKPLPRCFRVNTLKGTISEITKRLMKKGFQFTRVPWAREGFCLTREP
FSITSTPEYLTGLIYIQEASSMYPPVALDPKPGEIVADMAAAPGGKTSYMAQLMENKGLIFAFDVDEERLKETRLNLSRL
GVLNTILIHKSSLYIDELNMSFDKILLDAPCTGSGTIHKNPERKHNRTLDDIKFCQGLQMKMIEKALEVLKPGGILVYST
CSLEPEENEFVIQWALDNFDIELLPLRYGEPALINPFGIELSPEIKKARRFYPDTHKTSGFFIAKIRKKG
>Mature_310_residues
MLEKLFSLGYSKTFAERYYQLWGERAIRIAEAMEKPLPRCFRVNTLKGTISEITKRLMKKGFQFTRVPWAREGFCLTREP
FSITSTPEYLTGLIYIQEASSMYPPVALDPKPGEIVADMAAAPGGKTSYMAQLMENKGLIFAFDVDEERLKETRLNLSRL
GVLNTILIHKSSLYIDELNMSFDKILLDAPCTGSGTIHKNPERKHNRTLDDIKFCQGLQMKMIEKALEVLKPGGILVYST
CSLEPEENEFVIQWALDNFDIELLPLRYGEPALINPFGIELSPEIKKARRFYPDTHKTSGFFIAKIRKKG

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=289, Percent_Identity=41.8685121107266, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI76150623, Length=289, Percent_Identity=41.8685121107266, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI270288818, Length=294, Percent_Identity=32.312925170068, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI270288816, Length=294, Percent_Identity=32.312925170068, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI8922322, Length=294, Percent_Identity=32.312925170068, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI23199998, Length=294, Percent_Identity=32.312925170068, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI32698918, Length=263, Percent_Identity=33.4600760456274, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI39995082, Length=289, Percent_Identity=30.7958477508651, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI301336155, Length=268, Percent_Identity=29.4776119402985, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI11545785, Length=317, Percent_Identity=27.1293375394322, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87081985, Length=291, Percent_Identity=37.1134020618557, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI2367212, Length=301, Percent_Identity=30.8970099667774, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17536757, Length=310, Percent_Identity=35.4838709677419, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25143471, Length=279, Percent_Identity=28.3154121863799, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71998419, Length=297, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534021, Length=219, Percent_Identity=30.1369863013699, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=312, Percent_Identity=37.8205128205128, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319447, Length=271, Percent_Identity=32.1033210332103, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324306, Length=204, Percent_Identity=25.9803921568627, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI22024126, Length=285, Percent_Identity=36.140350877193, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21355201, Length=210, Percent_Identity=37.6190476190476, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356513, Length=239, Percent_Identity=34.7280334728033, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24668781, Length=239, Percent_Identity=34.7280334728033, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19920494, Length=180, Percent_Identity=33.8888888888889, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356579, Length=225, Percent_Identity=29.3333333333333, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 35377; Mature: 35377

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKLFSLGYSKTFAERYYQLWGERAIRIAEAMEKPLPRCFRVNTLKGTISEITKRLMKK
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHC
GFQFTRVPWAREGFCLTREPFSITSTPEYLTGLIYIQEASSMYPPVALDPKPGEIVADMA
CCEEEECCCCCCCEEEECCCCCCCCCHHHHEEEEEEECCCCCCCCEEECCCCCHHHHHHH
AAPGGKTSYMAQLMENKGLIFAFDVDEERLKETRLNLSRLGVLNTILIHKSSLYIDELNM
CCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHHCCCCEEEECCCC
SFDKILLDAPCTGSGTIHKNPERKHNRTLDDIKFCQGLQMKMIEKALEVLKPGGILVYST
CHHHEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEE
CSLEPEENEFVIQWALDNFDIELLPLRYGEPALINPFGIELSPEIKKARRFYPDTHKTSG
ECCCCCCCCEEEEEEECCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCC
FFIAKIRKKG
EEEEEEECCC
>Mature Secondary Structure
MLEKLFSLGYSKTFAERYYQLWGERAIRIAEAMEKPLPRCFRVNTLKGTISEITKRLMKK
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHC
GFQFTRVPWAREGFCLTREPFSITSTPEYLTGLIYIQEASSMYPPVALDPKPGEIVADMA
CCEEEECCCCCCCEEEECCCCCCCCCHHHHEEEEEEECCCCCCCCEEECCCCCHHHHHHH
AAPGGKTSYMAQLMENKGLIFAFDVDEERLKETRLNLSRLGVLNTILIHKSSLYIDELNM
CCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHHCCCCEEEECCCC
SFDKILLDAPCTGSGTIHKNPERKHNRTLDDIKFCQGLQMKMIEKALEVLKPGGILVYST
CHHHEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEE
CSLEPEENEFVIQWALDNFDIELLPLRYGEPALINPFGIELSPEIKKARRFYPDTHKTSG
ECCCCCCCCEEEEEEECCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCC
FFIAKIRKKG
EEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA