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The map label for this gene is yoaE [H]

Identifier: 18977614

GI number: 18977614

Start: 1175771

End: 1177696

Strand: Reverse

Name: yoaE [H]

Synonym: PF1242

Alternate gene names: 18977614

Gene position: 1177696-1175771 (Counterclockwise)

Preceding gene: 18977615

Following gene: 18977611

Centisome position: 61.72

GC content: 39.67

Gene sequence:

>1926_bases
ATGTTCTCAGCTTGCATGAGAGACTGCTATGATACATGTGCAATTATAAGCGAGTTTAAGAACGGTAAACTAACTGTAAA
AGGCAATCCGAAGCATCCAATAACAAGAGGATTTCTATGCCCAAAAGGAGCCTTGTTGCCAAAGTGGTTTCACTCTCCTG
AAAGGTTAAAAACTCCCCTGATTAGAAAAGGGAAAAGAGGAGAGAACAACTTTGCAGAAGTTTCTTGGGAGGAAGCAATT
AAACTTGTGGCCAAAAAGCTGAAAGAGACAATAGAAAAATTCGGAAGTGAGAGCATCCTTGTATACAGATACGCTGGAGA
TAGAGGAGTTGTTAACTACAACTTCCCACTGAGGTTATTTCACTATTTAAATGCAAGGACAATTGAAAGCGGAATTTGCG
ATAGAGCTGGTCAAGAGGCATTGAAAGATGTCTATGGAACAGCTGTTGGCCTTGATCCCGAAGAAATTCCCAATCATAAG
TTAATTGTTTATTGGGGCATAAATGCATTTTGGACTAATCTCCATGGCTTTATGCTTGCCAAGAGGAACAACGTAGAAAT
TTGGACGGTTGATGTTATTAGAACTGAAACTGCAAAGAGGAGTTCTAAGTTCTTCCAAATTAAGCCAGACACTGATGTTT
TATTTGCACTGGGTATCGCAAAAGTTATGATAGAGAATGGATGGTACGACAAAGAATTCGTGAAAGAAAACGTGTATGGA
TTTGAAGAATTCAAGAATTACATAAAAACATTATCCCTTGATTACATAAGTAAAGAAACAGGAATTGAGGTTGAGAAGAT
ATTTGAATTTGCTCAGGAATTTTGGGAGAAGAGAGGGATAATTCACATAGGATATGGATTCCAAAGATCACTGGCTGGAG
GAGAGGGTGTTAGAGCAGTCTCCCTTCTTCCAGCTCTTGTAGGCCACAAGTTTGGCTTCATTTATGACATGGTCACAATA
GATAAGAGCTATGCTGAAGGAAAGTTCTTAAGAACAGAGCCAGAGAATCCTCTACCTCAAATGGAGCTCGCGGAAGCAAT
TGAGAGCGGAGAGATAAAATTCCTCTACATCTATAATTCAAATCCTTTGGCTAGTTATCCAAATCAGAACAGATTAAGGA
GGGCTCTTGAAAATGCAGATATTTTCGTTGTTACCCACGACATCTTCATGACAGACACCGCTTTATACTCGGATGTAGTC
TTACCTGCCAACACATTCTTTGAAAGATTTGACATAGTTGACTCCTATTATCATAGGTATGTGCTATTAAATGAGCCCGT
GGCCAAAGGCCCTGGGAAAAGCAACAGTGAAGTAACGAGATTGCTTGCAAAGGAACTCGGTATTAATAACCCATATATAT
ACGAAAATGACGAAGAAGTCGTTAAAAAAGTCCTGGAAATTAATGGTATTAGTTTTGAAGAGCTAAAAGAGAAAGGCTTC
GTAAAAGTTCCCCAAAAGGAAAGAGTTTGGCCCACTCCCAGTGGAAAAATTGAGTTTTACTCCCAAAGAGCTGTAAAGAG
AGGTCTGTCTCCTTTCCCCACTTACAAGAAGTTCAGAGGAAAATACCCCCTAAGGTTACTAAGCCCCACATATAGAATGA
CAATAACAAGCCAGTACCACAATACCTATGGGATAATAGATGAAAAAATTTACATGAATCCCAAGGATGCCCAGGAGAGG
AAAATAAAAGATGGGGATAAAGTCATTGTTTTCAATGAATATGGAAGGGTAATAACAAGGGCAAAGATAACCGAGGACGT
TCCAAGAGGAGTTGTTGTCATGTACAAAGCTTTCTGGCCTTCACTCCTTGGCTGGAACGTTAACTTCCTAACAACAGACA
AAAAAGTAGAAGAGTATGGAAAGGCCTCAGCTTTCCACTCAACATGGGTTGATGTTACTCTATATAGTGAACCTGAGGAA
GGGTAG

Upstream 100 bases:

>100_bases
TGTAGAAGTGTTAGAGAGTGAAGAGGACTTTGAAAAAGGTAGATATGTAGTTCGATACATTTTAGAAGATCAAGGCAGAG
ATTAGAGAGGGAGAAGAAAA

Downstream 100 bases:

>100_bases
TGATTTTCACTGGCTCTCCAAAAGCCCTGTCTCCAGCATCCCCCAATCCTGGGAGAATATAACCTTTATCATTTAATTCC
CTGTCTATCTTGGCGACGAA

Product: dehydrogenase subunit alpha

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MFSACMRDCYDTCAIISEFKNGKLTVKGNPKHPITRGFLCPKGALLPKWFHSPERLKTPLIRKGKRGENNFAEVSWEEAI
KLVAKKLKETIEKFGSESILVYRYAGDRGVVNYNFPLRLFHYLNARTIESGICDRAGQEALKDVYGTAVGLDPEEIPNHK
LIVYWGINAFWTNLHGFMLAKRNNVEIWTVDVIRTETAKRSSKFFQIKPDTDVLFALGIAKVMIENGWYDKEFVKENVYG
FEEFKNYIKTLSLDYISKETGIEVEKIFEFAQEFWEKRGIIHIGYGFQRSLAGGEGVRAVSLLPALVGHKFGFIYDMVTI
DKSYAEGKFLRTEPENPLPQMELAEAIESGEIKFLYIYNSNPLASYPNQNRLRRALENADIFVVTHDIFMTDTALYSDVV
LPANTFFERFDIVDSYYHRYVLLNEPVAKGPGKSNSEVTRLLAKELGINNPYIYENDEEVVKKVLEINGISFEELKEKGF
VKVPQKERVWPTPSGKIEFYSQRAVKRGLSPFPTYKKFRGKYPLRLLSPTYRMTITSQYHNTYGIIDEKIYMNPKDAQER
KIKDGDKVIVFNEYGRVITRAKITEDVPRGVVVMYKAFWPSLLGWNVNFLTTDKKVEEYGKASAFHSTWVDVTLYSEPEE
G

Sequences:

>Translated_641_residues
MFSACMRDCYDTCAIISEFKNGKLTVKGNPKHPITRGFLCPKGALLPKWFHSPERLKTPLIRKGKRGENNFAEVSWEEAI
KLVAKKLKETIEKFGSESILVYRYAGDRGVVNYNFPLRLFHYLNARTIESGICDRAGQEALKDVYGTAVGLDPEEIPNHK
LIVYWGINAFWTNLHGFMLAKRNNVEIWTVDVIRTETAKRSSKFFQIKPDTDVLFALGIAKVMIENGWYDKEFVKENVYG
FEEFKNYIKTLSLDYISKETGIEVEKIFEFAQEFWEKRGIIHIGYGFQRSLAGGEGVRAVSLLPALVGHKFGFIYDMVTI
DKSYAEGKFLRTEPENPLPQMELAEAIESGEIKFLYIYNSNPLASYPNQNRLRRALENADIFVVTHDIFMTDTALYSDVV
LPANTFFERFDIVDSYYHRYVLLNEPVAKGPGKSNSEVTRLLAKELGINNPYIYENDEEVVKKVLEINGISFEELKEKGF
VKVPQKERVWPTPSGKIEFYSQRAVKRGLSPFPTYKKFRGKYPLRLLSPTYRMTITSQYHNTYGIIDEKIYMNPKDAQER
KIKDGDKVIVFNEYGRVITRAKITEDVPRGVVVMYKAFWPSLLGWNVNFLTTDKKVEEYGKASAFHSTWVDVTLYSEPEE
G
>Mature_641_residues
MFSACMRDCYDTCAIISEFKNGKLTVKGNPKHPITRGFLCPKGALLPKWFHSPERLKTPLIRKGKRGENNFAEVSWEEAI
KLVAKKLKETIEKFGSESILVYRYAGDRGVVNYNFPLRLFHYLNARTIESGICDRAGQEALKDVYGTAVGLDPEEIPNHK
LIVYWGINAFWTNLHGFMLAKRNNVEIWTVDVIRTETAKRSSKFFQIKPDTDVLFALGIAKVMIENGWYDKEFVKENVYG
FEEFKNYIKTLSLDYISKETGIEVEKIFEFAQEFWEKRGIIHIGYGFQRSLAGGEGVRAVSLLPALVGHKFGFIYDMVTI
DKSYAEGKFLRTEPENPLPQMELAEAIESGEIKFLYIYNSNPLASYPNQNRLRRALENADIFVVTHDIFMTDTALYSDVV
LPANTFFERFDIVDSYYHRYVLLNEPVAKGPGKSNSEVTRLLAKELGINNPYIYENDEEVVKKVLEINGISFEELKEKGF
VKVPQKERVWPTPSGKIEFYSQRAVKRGLSPFPTYKKFRGKYPLRLLSPTYRMTITSQYHNTYGIIDEKIYMNPKDAQER
KIKDGDKVIVFNEYGRVITRAKITEDVPRGVVVMYKAFWPSLLGWNVNFLTTDKKVEEYGKASAFHSTWVDVTLYSEPEE
G

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=765, Percent_Identity=27.1895424836601, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI171474008, Length=761, Percent_Identity=26.4126149802891, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787870, Length=727, Percent_Identity=27.2352132049519, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI3868721, Length=658, Percent_Identity=24.9240121580547, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI145693196, Length=718, Percent_Identity=23.6768802228412, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87081994, Length=723, Percent_Identity=23.9280774550484, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787231, Length=738, Percent_Identity=22.6287262872629, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI3868720, Length=263, Percent_Identity=27.7566539923954, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 73801; Mature: 73801

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSACMRDCYDTCAIISEFKNGKLTVKGNPKHPITRGFLCPKGALLPKWFHSPERLKTPL
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCHH
IRKGKRGENNFAEVSWEEAIKLVAKKLKETIEKFGSESILVYRYAGDRGVVNYNFPLRLF
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
HYLNARTIESGICDRAGQEALKDVYGTAVGLDPEEIPNHKLIVYWGINAFWTNLHGFMLA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCHHHHHCCCEEEEE
KRNNVEIWTVDVIRTETAKRSSKFFQIKPDTDVLFALGIAKVMIENGWYDKEFVKENVYG
ECCCEEEEEEEEEECCHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
FEEFKNYIKTLSLDYISKETGIEVEKIFEFAQEFWEKRGIIHIGYGFQRSLAGGEGVRAV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCCCHHH
SLLPALVGHKFGFIYDMVTIDKSYAEGKFLRTEPENPLPQMELAEAIESGEIKFLYIYNS
HHHHHHHCCHHHHEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEEEECC
NPLASYPNQNRLRRALENADIFVVTHDIFMTDTALYSDVVLPANTFFERFDIVDSYYHRY
CCCCCCCCHHHHHHHHCCCCEEEEEECHHHCCHHHHHCEECCHHHHHHHHHHHHHHHHEE
VLLNEPVAKGPGKSNSEVTRLLAKELGINNPYIYENDEEVVKKVLEINGISFEELKEKGF
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHCCC
VKVPQKERVWPTPSGKIEFYSQRAVKRGLSPFPTYKKFRGKYPLRLLSPTYRMTITSQYH
EECCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCEEEEEEECCC
NTYGIIDEKIYMNPKDAQERKIKDGDKVIVFNEYGRVITRAKITEDVPRGVVVMYKAFWP
CCCCEECCEEEECCCCHHHHHCCCCCEEEEECCCCCEEEHHHHHHCCCCCEEEEHHHHHH
SLLGWNVNFLTTDKKVEEYGKASAFHSTWVDVTLYSEPEEG
HHHCCCEEEEECCHHHHHHCCHHCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MFSACMRDCYDTCAIISEFKNGKLTVKGNPKHPITRGFLCPKGALLPKWFHSPERLKTPL
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCHH
IRKGKRGENNFAEVSWEEAIKLVAKKLKETIEKFGSESILVYRYAGDRGVVNYNFPLRLF
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
HYLNARTIESGICDRAGQEALKDVYGTAVGLDPEEIPNHKLIVYWGINAFWTNLHGFMLA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCHHHHHCCCEEEEE
KRNNVEIWTVDVIRTETAKRSSKFFQIKPDTDVLFALGIAKVMIENGWYDKEFVKENVYG
ECCCEEEEEEEEEECCHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
FEEFKNYIKTLSLDYISKETGIEVEKIFEFAQEFWEKRGIIHIGYGFQRSLAGGEGVRAV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCCCHHH
SLLPALVGHKFGFIYDMVTIDKSYAEGKFLRTEPENPLPQMELAEAIESGEIKFLYIYNS
HHHHHHHCCHHHHEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEEEECC
NPLASYPNQNRLRRALENADIFVVTHDIFMTDTALYSDVVLPANTFFERFDIVDSYYHRY
CCCCCCCCHHHHHHHHCCCCEEEEEECHHHCCHHHHHCEECCHHHHHHHHHHHHHHHHEE
VLLNEPVAKGPGKSNSEVTRLLAKELGINNPYIYENDEEVVKKVLEINGISFEELKEKGF
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCHHHHHHCCC
VKVPQKERVWPTPSGKIEFYSQRAVKRGLSPFPTYKKFRGKYPLRLLSPTYRMTITSQYH
EECCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCEEEEEEECCC
NTYGIIDEKIYMNPKDAQERKIKDGDKVIVFNEYGRVITRAKITEDVPRGVVVMYKAFWP
CCCCEECCEEEECCCCHHHHHCCCCCEEEEECCCCCEEEHHHHHHCCCCCEEEEHHHHHH
SLLGWNVNFLTTDKKVEEYGKASAFHSTWVDVTLYSEPEEG
HHHCCCEEEEECCHHHHHHCCHHCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]