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The map label for this gene is nth [H]

Identifier: 18977601

GI number: 18977601

Start: 1162991

End: 1163668

Strand: Reverse

Name: nth [H]

Synonym: PF1229

Alternate gene names: 18977601

Gene position: 1163668-1162991 (Counterclockwise)

Preceding gene: 18977602

Following gene: 33359552

Centisome position: 60.98

GC content: 38.94

Gene sequence:

>678_bases
TTGGAGAGGAAAAGGTTAAGATCCTCAAGCTTCAATGAAACCTTAGAAGAAAAGAAAGCTAGAGCTCAAAGGATTATTGA
AATTCTTAAAAGAGAATATCCCAGGGAGAGACATGTCTCTGGAGATCCCTATAGAACACTAATAAGATGTATAATTTCCC
AAAGGAATAGGGATGAAGTCACTGATAAAGTTAGTGAGGAGCTATTTAAAAGGTATAAAAGCATAGAAGAAATCGCCAAT
GAGTCCGTAGAAAATATGCAAGAATTCCTCAGAAAGCAAAAGGTAGGACTTTGGAAAAATAAGGGGAAATGGATCGTGGA
AGCCTCAAGAATTATTCTCTACAAATATGGGGGAAAAGTGCCCAATACTCTCGAAGAGCTGATGAAACTCCCAGGAATTG
GAAGGAAGTGTGCTAACATCGTTCTAGCCTACGGCTTTGGAAAGCCAGCGATTCCCGTTGATACCCACGTATATAGGATA
AGTAGAAGATTGGGGCTAGCTCCTATAAATTCAACGCCAGAAAAAGTTGAAGAAATACTAAAAACCTTAATCCCCGTAGA
AGAATGGATATACGTTAATCATGCTATGGTTGACCATGGGAAAAGCATATGTAGACCAATAAAGCCTAAGTGTGAGTTAT
GCCCTTTAAATGAGCTATGTCCGAAGATAGGAGTTTAG

Upstream 100 bases:

>100_bases
AGTGAGGTTCTAATATTAAACCATAACTTTGAGGAGATAAGAAGAATAGAGTTCGAAAATTTGAAAAAGATAGTAGTCAA
AGGAGATTTGGTGGTATTGA

Downstream 100 bases:

>100_bases
GAAAACCAATAGCTCATATCCAAAAGTTTTCTCTTCAAATCTTTTCCATAGATATAACCTAATAAAATTCCAACAATTAA
GCCACCAAAGTGGGCAACGT

Product: glycosylase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MERKRLRSSSFNETLEEKKARAQRIIEILKREYPRERHVSGDPYRTLIRCIISQRNRDEVTDKVSEELFKRYKSIEEIAN
ESVENMQEFLRKQKVGLWKNKGKWIVEASRIILYKYGGKVPNTLEELMKLPGIGRKCANIVLAYGFGKPAIPVDTHVYRI
SRRLGLAPINSTPEKVEEILKTLIPVEEWIYVNHAMVDHGKSICRPIKPKCELCPLNELCPKIGV

Sequences:

>Translated_225_residues
MERKRLRSSSFNETLEEKKARAQRIIEILKREYPRERHVSGDPYRTLIRCIISQRNRDEVTDKVSEELFKRYKSIEEIAN
ESVENMQEFLRKQKVGLWKNKGKWIVEASRIILYKYGGKVPNTLEELMKLPGIGRKCANIVLAYGFGKPAIPVDTHVYRI
SRRLGLAPINSTPEKVEEILKTLIPVEEWIYVNHAMVDHGKSICRPIKPKCELCPLNELCPKIGV
>Mature_225_residues
MERKRLRSSSFNETLEEKKARAQRIIEILKREYPRERHVSGDPYRTLIRCIISQRNRDEVTDKVSEELFKRYKSIEEIAN
ESVENMQEFLRKQKVGLWKNKGKWIVEASRIILYKYGGKVPNTLEELMKLPGIGRKCANIVLAYGFGKPAIPVDTHVYRI
SRRLGLAPINSTPEKVEEILKTLIPVEEWIYVNHAMVDHGKSICRPIKPKCELCPLNELCPKIGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=180, Percent_Identity=32.2222222222222, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI115298654, Length=158, Percent_Identity=25.9493670886076, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI115298652, Length=158, Percent_Identity=25.9493670886076, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI115298648, Length=158, Percent_Identity=25.9493670886076, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI115298650, Length=158, Percent_Identity=25.9493670886076, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI6912520, Length=158, Percent_Identity=25.9493670886076, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI190358497, Length=158, Percent_Identity=25.9493670886076, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=32.1951219512195, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789331, Length=190, Percent_Identity=27.8947368421053, Blast_Score=78, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=35.7541899441341, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324530, Length=184, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=32.2222222222222, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26158; Mature: 26158

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKRLRSSSFNETLEEKKARAQRIIEILKREYPRERHVSGDPYRTLIRCIISQRNRDEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHCCCHHHH
TDKVSEELFKRYKSIEEIANESVENMQEFLRKQKVGLWKNKGKWIVEASRIILYKYGGKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEECCCCCC
PNTLEELMKLPGIGRKCANIVLAYGFGKPAIPVDTHVYRISRRLGLAPINSTPEKVEEIL
CHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
KTLIPVEEWIYVNHAMVDHGKSICRPIKPKCELCPLNELCPKIGV
HHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MERKRLRSSSFNETLEEKKARAQRIIEILKREYPRERHVSGDPYRTLIRCIISQRNRDEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHCCCHHHH
TDKVSEELFKRYKSIEEIANESVENMQEFLRKQKVGLWKNKGKWIVEASRIILYKYGGKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEECCCCCC
PNTLEELMKLPGIGRKCANIVLAYGFGKPAIPVDTHVYRISRRLGLAPINSTPEKVEEIL
CHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
KTLIPVEEWIYVNHAMVDHGKSICRPIKPKCELCPLNELCPKIGV
HHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]