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The map label for this gene is ycbU [H]

Identifier: 18977438

GI number: 18977438

Start: 1018574

End: 1019818

Strand: Direct

Name: ycbU [H]

Synonym: PF1066

Alternate gene names: 18977438

Gene position: 1018574-1019818 (Clockwise)

Preceding gene: 18977436

Following gene: 18977440

Centisome position: 53.38

GC content: 42.97

Gene sequence:

>1245_bases
ATGAAACTATCTCCCACAGCTCAGCCATTCTTACACCTCCACAACTTATTTATCCTTCACCAAAAAGTTTTAGTGGTGAA
GCCAATGAAGGAATTATTCCCAGGACTTAAGAGGTTTAGGGCATATCTCAACACTGCCGGCCTGGGTTTAATGCCCTTAA
CAGTTTACCAAAAAGTGAATGAATTTCTCTTAGACGTTATAAACTACGAGGATGGAATAAACGCTGTTGAGATTCTTGAT
GAGCTCTACTTTAACCCCATGTTGAAAAAGGCCGCGAAGCTTATGAAAACTAACCCCGAGAACATAACAGTGAGTCTTCA
AACTACAGATGGCCTAAAGAGAATTCTAACATCTCTAACCCCCAAGAAAGGCATGAAGATTGTCTCCTTTGACCTAGAAT
TCCCCACGATTTCTGCCATATTAAAAAGCTATGCCAAAAGGCATGGTTTGGAAGTTAAAGTTATCGGAAACAAAGGGGGA
TTATATTACATGGAGGATGTAGAGAGGGTAATAGACGATAACACCTTTGCCGTGGTTTTTAGTGATGTGCAGTGGATTAC
TGGGCAGAGAATGCCAACGAAAGAGATTGCCGGAGTTGCTCACGAGCATGGAGCCTGGGTTATTGTGGATGCAGTTCAAT
CCCTGGGAGCATTAGAGGTGTATCCAGAGAAGATGGAAGTAGATGCCCTCGTGGCAGGGGGAGAGAAGTGGCTCCTTAAC
CCAAATACGGGAAGTGGAGTCATGTGGATATCGGAGGAATTAATTGAAGTTAGCGAACCTATATTGGGGCTTTTAAACAC
CCAACCAGAAGTTCCATGGTCAGAGTGGTGGGGAGATGAGAGAAAGGATGTTTGGGATATTCTTCCTGTAAGAAAAGACG
CTAGAAAGCTTGATCCTGGAACTCCTCCTTACGTTAGCATAGTGGCCCTAGATGCTTCCTTAGAGCTCATAAATAGTGTG
GGAATTGAAAAAATCGAGAAGCACGACATGAAGCTAGCAAAAAGGGTTAGGGAGTACGCATACGAACTAGGTGTAACTGC
TTTAGGTTCATCACAGATAACTCTTCTGAGATTAGGAGTGGACTATGTGAAGCAGAGAGAAATAGTGAAAAATTTGGAAG
AGAAAGGAATTGTGGTCTCCTTAAGGGGAGCAAAGGGAATCTTTGGCATAAGAGTCTCGCCACACCTCTACAATACTGAG
GATGACATTGTCACGCTGTTTGATGAACTAAGCAAGTTTTTTTGA

Upstream 100 bases:

>100_bases
ACCGCAAATGCTATCGTGTTAACTTCCTCCTTCCCCTCAACTTGCAAACCGTTGCTGGATTTGTCAGGATATTCAGAAAT
CTTGAGGTATTCATCTAGGA

Downstream 100 bases:

>100_bases
GAGTTAGATTTATTCTTTTTATATCAACTAAAAGACTTTGGCACTCTTAACTTTAGTATGGAAAATTATCTCTAATTCAT
GTTATTTAAAAAATTAACAG

Product: putative aminotransferase

Products: hydrogen selenide; @ALAN01.txt*l-alanine!; acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKLSPTAQPFLHLHNLFILHQKVLVVKPMKELFPGLKRFRAYLNTAGLGLMPLTVYQKVNEFLLDVINYEDGINAVEILD
ELYFNPMLKKAAKLMKTNPENITVSLQTTDGLKRILTSLTPKKGMKIVSFDLEFPTISAILKSYAKRHGLEVKVIGNKGG
LYYMEDVERVIDDNTFAVVFSDVQWITGQRMPTKEIAGVAHEHGAWVIVDAVQSLGALEVYPEKMEVDALVAGGEKWLLN
PNTGSGVMWISEELIEVSEPILGLLNTQPEVPWSEWWGDERKDVWDILPVRKDARKLDPGTPPYVSIVALDASLELINSV
GIEKIEKHDMKLAKRVREYAYELGVTALGSSQITLLRLGVDYVKQREIVKNLEEKGIVVSLRGAKGIFGIRVSPHLYNTE
DDIVTLFDELSKFF

Sequences:

>Translated_414_residues
MKLSPTAQPFLHLHNLFILHQKVLVVKPMKELFPGLKRFRAYLNTAGLGLMPLTVYQKVNEFLLDVINYEDGINAVEILD
ELYFNPMLKKAAKLMKTNPENITVSLQTTDGLKRILTSLTPKKGMKIVSFDLEFPTISAILKSYAKRHGLEVKVIGNKGG
LYYMEDVERVIDDNTFAVVFSDVQWITGQRMPTKEIAGVAHEHGAWVIVDAVQSLGALEVYPEKMEVDALVAGGEKWLLN
PNTGSGVMWISEELIEVSEPILGLLNTQPEVPWSEWWGDERKDVWDILPVRKDARKLDPGTPPYVSIVALDASLELINSV
GIEKIEKHDMKLAKRVREYAYELGVTALGSSQITLLRLGVDYVKQREIVKNLEEKGIVVSLRGAKGIFGIRVSPHLYNTE
DDIVTLFDELSKFF
>Mature_414_residues
MKLSPTAQPFLHLHNLFILHQKVLVVKPMKELFPGLKRFRAYLNTAGLGLMPLTVYQKVNEFLLDVINYEDGINAVEILD
ELYFNPMLKKAAKLMKTNPENITVSLQTTDGLKRILTSLTPKKGMKIVSFDLEFPTISAILKSYAKRHGLEVKVIGNKGG
LYYMEDVERVIDDNTFAVVFSDVQWITGQRMPTKEIAGVAHEHGAWVIVDAVQSLGALEVYPEKMEVDALVAGGEKWLLN
PNTGSGVMWISEELIEVSEPILGLLNTQPEVPWSEWWGDERKDVWDILPVRKDARKLDPGTPPYVSIVALDASLELINSV
GIEKIEKHDMKLAKRVREYAYELGVTALGSSQITLLRLGVDYVKQREIVKNLEEKGIVVSLRGAKGIFGIRVSPHLYNTE
DDIVTLFDELSKFF

Specific function: Cysteine Desulfurases Mobilize The Sulfur From L- Cysteine To Yield L-Alanine, An Essential Step In Sulfur Metabolism For Biosynthesis Of A Variety Of Sulfur-Containing Biomolecules. Component Of The Suf Operon, Which Is Activated And Required Under Speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1787970, Length=321, Percent_Identity=24.9221183800623, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.8.1.7 [C]

Molecular weight: Translated: 46603; Mature: 46603

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSPTAQPFLHLHNLFILHQKVLVVKPMKELFPGLKRFRAYLNTAGLGLMPLTVYQKVN
CCCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EFLLDVINYEDGINAVEILDELYFNPMLKKAAKLMKTNPENITVSLQTTDGLKRILTSLT
HHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCC
PKKGMKIVSFDLEFPTISAILKSYAKRHGLEVKVIGNKGGLYYMEDVERVIDDNTFAVVF
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCCEEEEE
SDVQWITGQRMPTKEIAGVAHEHGAWVIVDAVQSLGALEVYPEKMEVDALVAGGEKWLLN
ECHHHHCCCCCCHHHHHCCHHCCCCEEEEEHHHHCCCEEECCCHHCEEEEEECCCEEEEC
PNTGSGVMWISEELIEVSEPILGLLNTQPEVPWSEWWGDERKDVWDILPVRKDARKLDPG
CCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCHHHHHCCCC
TPPYVSIVALDASLELINSVGIEKIEKHDMKLAKRVREYAYELGVTALGSSQITLLRLGV
CCCEEEEEEECHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCH
DYVKQREIVKNLEEKGIVVSLRGAKGIFGIRVSPHLYNTEDDIVTLFDELSKFF
HHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSPTAQPFLHLHNLFILHQKVLVVKPMKELFPGLKRFRAYLNTAGLGLMPLTVYQKVN
CCCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EFLLDVINYEDGINAVEILDELYFNPMLKKAAKLMKTNPENITVSLQTTDGLKRILTSLT
HHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCC
PKKGMKIVSFDLEFPTISAILKSYAKRHGLEVKVIGNKGGLYYMEDVERVIDDNTFAVVF
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCCEEEEE
SDVQWITGQRMPTKEIAGVAHEHGAWVIVDAVQSLGALEVYPEKMEVDALVAGGEKWLLN
ECHHHHCCCCCCHHHHHCCHHCCCCEEEEEHHHHCCCEEECCCHHCEEEEEECCCEEEEC
PNTGSGVMWISEELIEVSEPILGLLNTQPEVPWSEWWGDERKDVWDILPVRKDARKLDPG
CCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHCCCCHHHHHCCCC
TPPYVSIVALDASLELINSVGIEKIEKHDMKLAKRVREYAYELGVTALGSSQITLLRLGV
CCCEEEEEEECHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCH
DYVKQREIVKNLEEKGIVVSLRGAKGIFGIRVSPHLYNTEDDIVTLFDELSKFF
HHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-selenocysteine; reduced acceptor [C]

Specific reaction: L-selenocysteine + reduced acceptor = hydrogen selenide + @ALAN01.txt*l-alanine! + acceptor [C]

General reaction: Elimination of H2S or RSH [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377; 7704254; 1353026 [H]