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The map label for this gene is acaA [H]

Identifier: 18977344

GI number: 18977344

Start: 931235

End: 932287

Strand: Direct

Name: acaA [H]

Synonym: PF0972

Alternate gene names: 18977344

Gene position: 931235-932287 (Clockwise)

Preceding gene: 18977324

Following gene: 18977345

Centisome position: 48.8

GC content: 44.54

Gene sequence:

>1053_bases
ATGAGAAAGGTAATGAAGCCAATGAAAGACGTTGGTATTGTTGGGTATGGAGCATACGTGCCAATGTATAGGATAAGGAA
TGAAGAAATAGGAAGAGTATGGGGGGTCTCAAGCTTTCCGATAGAAGAAAAGGCTGTACCTGGGCTTGATGAAGACGCTA
TAACGATAGGAATAGAAGCTGCTAGAAACGCCTTAAAGAGAGCTAAGATAGATCCTCAAAAAATAAGGGCCATTTGGTTT
GGTACTGAAAGTAAGCCCTATGCAGTAAAGCCTTCAGCTACAATAATAGCTGAAGCTATTGGTGCAACTCCAGATCTTGA
AGCTGCTGATTTTGAATTCGCTTGTAAGGCTGGCACTGAGGCTCTGCAAGCTGCCATAGGTTTTGTAGGTTCGGGCATGG
CTGAGTATGCAATGGCCATAGGTGCTGACACTGCCCAGGGAAGGCCCGGAGATCACTTAGAGTTCACAGCTGGGGCAGGA
GGAGCAGCTTTCATTGTGGGAGAAAAGAGCAATGAGAGTGTAGCATATTTTGAGGGGAGCTACTCTTACGTTACAGACAC
TCCCGATTTCTGGAGAAGGCAACATGAACACTACCCCAGGCATGGAAACAGGTTTACTGGGGAGCCAGCATACTTCCACC
ATATAATTAATGCAGCCAAGACCCTTATGGAAGAGTTAGGCCTTAAGCCAAGTGACTTTGATTATGCAGTATTTCACCAA
CCAAATGTAAAGTTCCCATTGACAGTTGCTAAGATTCTTGGGATTCCTAAAGAGAAAGTTCTACCGGGATTGCTAACAGG
GAGAATAGGAAATACATACAGCGGTGCAACAATGGTTGGAGTTTCTGCTGTTTTGGACATAGCAAAGCCCGGGGATAGAA
TTCTCTGGGTCTCGTTTGGTTCTGGAGCAGGAAGCAATGCATTTAGTATAGTTGTTCAGGATGCCATAGAGGAGAAGAGA
GATTTAGCGCCAAAAGTTGAGGATTATGTGAAGAGGAGAAAAGTCATTGATTACGCTCTCTATGCAAAGGCTAGAAGAAA
GTACATCTTGTAG

Upstream 100 bases:

>100_bases
TATCGACGATGAACGAAATAAAGTTAAGCTTTTACCGAAAGTCTTTTATAACTCCTCCTTTTTCTAGTAGGTGACACAGT
AATTCCCAGGGGGAGGTTAA

Downstream 100 bases:

>100_bases
AGGGGTGGGAGATATGAGAAAAGCAATAATAGTTGGAGTTGGAATGACTCCTGTTGGAGAACATTGGAGGTCTTCTCTTA
GGGATTTAGCCGTCGAAGCA

Product: hypothetical protein

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRKVMKPMKDVGIVGYGAYVPMYRIRNEEIGRVWGVSSFPIEEKAVPGLDEDAITIGIEAARNALKRAKIDPQKIRAIWF
GTESKPYAVKPSATIIAEAIGATPDLEAADFEFACKAGTEALQAAIGFVGSGMAEYAMAIGADTAQGRPGDHLEFTAGAG
GAAFIVGEKSNESVAYFEGSYSYVTDTPDFWRRQHEHYPRHGNRFTGEPAYFHHIINAAKTLMEELGLKPSDFDYAVFHQ
PNVKFPLTVAKILGIPKEKVLPGLLTGRIGNTYSGATMVGVSAVLDIAKPGDRILWVSFGSGAGSNAFSIVVQDAIEEKR
DLAPKVEDYVKRRKVIDYALYAKARRKYIL

Sequences:

>Translated_350_residues
MRKVMKPMKDVGIVGYGAYVPMYRIRNEEIGRVWGVSSFPIEEKAVPGLDEDAITIGIEAARNALKRAKIDPQKIRAIWF
GTESKPYAVKPSATIIAEAIGATPDLEAADFEFACKAGTEALQAAIGFVGSGMAEYAMAIGADTAQGRPGDHLEFTAGAG
GAAFIVGEKSNESVAYFEGSYSYVTDTPDFWRRQHEHYPRHGNRFTGEPAYFHHIINAAKTLMEELGLKPSDFDYAVFHQ
PNVKFPLTVAKILGIPKEKVLPGLLTGRIGNTYSGATMVGVSAVLDIAKPGDRILWVSFGSGAGSNAFSIVVQDAIEEKR
DLAPKVEDYVKRRKVIDYALYAKARRKYIL
>Mature_350_residues
MRKVMKPMKDVGIVGYGAYVPMYRIRNEEIGRVWGVSSFPIEEKAVPGLDEDAITIGIEAARNALKRAKIDPQKIRAIWF
GTESKPYAVKPSATIIAEAIGATPDLEAADFEFACKAGTEALQAAIGFVGSGMAEYAMAIGADTAQGRPGDHLEFTAGAG
GAAFIVGEKSNESVAYFEGSYSYVTDTPDFWRRQHEHYPRHGNRFTGEPAYFHHIINAAKTLMEELGLKPSDFDYAVFHQ
PNVKFPLTVAKILGIPKEKVLPGLLTGRIGNTYSGATMVGVSAVLDIAKPGDRILWVSFGSGAGSNAFSIVVQDAIEEKR
DLAPKVEDYVKRRKVIDYALYAKARRKYIL

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family [H]

Homologues:

Organism=Escherichia coli, GI1787333, Length=313, Percent_Identity=29.3929712460064, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013751
- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08545 ACP_syn_III; PF08541 ACP_syn_III_C [H]

EC number: 2.3.1.41

Molecular weight: Translated: 38201; Mature: 38201

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVMKPMKDVGIVGYGAYVPMYRIRNEEIGRVWGVSSFPIEEKAVPGLDEDAITIGIEA
CCHHHHHHHHCCEEEEHHHCCHHEECCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEHHH
ARNALKRAKIDPQKIRAIWFGTESKPYAVKPSATIIAEAIGATPDLEAADFEFACKAGTE
HHHHHHHCCCCHHHEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHH
ALQAAIGFVGSGMAEYAMAIGADTAQGRPGDHLEFTAGAGGAAFIVGEKSNESVAYFEGS
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEEEEEECC
YSYVTDTPDFWRRQHEHYPRHGNRFTGEPAYFHHIINAAKTLMEELGLKPSDFDYAVFHQ
CCEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNVKFPLTVAKILGIPKEKVLPGLLTGRIGNTYSGATMVGVSAVLDIAKPGDRILWVSFG
CCCCCCHHHHHHHCCCHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
SGAGSNAFSIVVQDAIEEKRDLAPKVEDYVKRRKVIDYALYAKARRKYIL
CCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKVMKPMKDVGIVGYGAYVPMYRIRNEEIGRVWGVSSFPIEEKAVPGLDEDAITIGIEA
CCHHHHHHHHCCEEEEHHHCCHHEECCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEHHH
ARNALKRAKIDPQKIRAIWFGTESKPYAVKPSATIIAEAIGATPDLEAADFEFACKAGTE
HHHHHHHCCCCHHHEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHH
ALQAAIGFVGSGMAEYAMAIGADTAQGRPGDHLEFTAGAGGAAFIVGEKSNESVAYFEGS
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEEEEEECC
YSYVTDTPDFWRRQHEHYPRHGNRFTGEPAYFHHIINAAKTLMEELGLKPSDFDYAVFHQ
CCEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNVKFPLTVAKILGIPKEKVLPGLLTGRIGNTYSGATMVGVSAVLDIAKPGDRILWVSFG
CCCCCCHHHHHHHCCCHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEC
SGAGSNAFSIVVQDAIEEKRDLAPKVEDYVKRRKVIDYALYAKARRKYIL
CCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]