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The map label for this gene is ndk

Identifier: 18977303

GI number: 18977303

Start: 894645

End: 895130

Strand: Reverse

Name: ndk

Synonym: PF0931

Alternate gene names: 18977303

Gene position: 895130-894645 (Counterclockwise)

Preceding gene: 18977304

Following gene: 33359527

Centisome position: 46.91

GC content: 42.59

Gene sequence:

>486_bases
ATGAACGAAGTTGAAAGAACATTGGTAATCATAAAGCCCGACGCAGTAGTTAGGGGTCTAATAGGTGAAATTATAAGCAG
GTTTGAGAAGAAAGGCCTCAAGATTGTTGGAATGAAGATGATCTGGATAGACAGGGAGTTGGCTGAGAAGCACTATGAGG
AGCACAAAGGAAAGCCCTTCTTTGAGGCTCTCATAGATTACATAACGAAAGCTCCAGTAGTTGTTATGGTGGTTGAGGGA
AGGTATGCAGTAGAAGTAGTTAGAAAGATGGCTGGAGCTACTGATCCAAAGGACGCAGCACCTGGGACAATTAGGGGAGA
TTATGGACTTGACATAGGAGATGCAATCTACAACGTGATTCATGCCAGTGATTCAAAGGAAAGTGCGGAGAGGGAAATAA
GCCTGTACTTTAAACCTGAAGAAATTTATGAATACTGCAAAGCTGCAGATTGGTTTTACAGGGAAAAGAAGCAGGCTAAA
TGCTGA

Upstream 100 bases:

>100_bases
CCCTTTGTCCTTGCAGGAATTTTCGTAGGCCTGGCATTCTTGGCATGGAAAGGGAGGAATTTACTTAAACCTTTGAGGGA
AAATAATGGGGGTGCTTTGG

Downstream 100 bases:

>100_bases
ACTCTATTATTTTTGCCCTTCCATTATTCTTTAACCACTCAAGGAGCTCCCTCGCAAATTTAAGTTTTTTCATTTTGCTC
TCCCATGCGGGGGTGTGATC

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNEVERTLVIIKPDAVVRGLIGEIISRFEKKGLKIVGMKMIWIDRELAEKHYEEHKGKPFFEALIDYITKAPVVVMVVEG
RYAVEVVRKMAGATDPKDAAPGTIRGDYGLDIGDAIYNVIHASDSKESAEREISLYFKPEEIYEYCKAADWFYREKKQAK
C

Sequences:

>Translated_161_residues
MNEVERTLVIIKPDAVVRGLIGEIISRFEKKGLKIVGMKMIWIDRELAEKHYEEHKGKPFFEALIDYITKAPVVVMVVEG
RYAVEVVRKMAGATDPKDAAPGTIRGDYGLDIGDAIYNVIHASDSKESAEREISLYFKPEEIYEYCKAADWFYREKKQAK
C
>Mature_161_residues
MNEVERTLVIIKPDAVVRGLIGEIISRFEKKGLKIVGMKMIWIDRELAEKHYEEHKGKPFFEALIDYITKAPVVVMVVEG
RYAVEVVRKMAGATDPKDAAPGTIRGDYGLDIGDAIYNVIHASDSKESAEREISLYFKPEEIYEYCKAADWFYREKKQAK
C

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family

Homologues:

Organism=Homo sapiens, GI66392203, Length=154, Percent_Identity=50.6493506493506, Blast_Score=158, Evalue=2e-39,
Organism=Homo sapiens, GI66392227, Length=154, Percent_Identity=50.6493506493506, Blast_Score=157, Evalue=3e-39,
Organism=Homo sapiens, GI66392205, Length=154, Percent_Identity=50.6493506493506, Blast_Score=157, Evalue=3e-39,
Organism=Homo sapiens, GI4505409, Length=154, Percent_Identity=50.6493506493506, Blast_Score=157, Evalue=3e-39,
Organism=Homo sapiens, GI66392192, Length=154, Percent_Identity=50.6493506493506, Blast_Score=157, Evalue=3e-39,
Organism=Homo sapiens, GI38045913, Length=154, Percent_Identity=49.3506493506493, Blast_Score=157, Evalue=3e-39,
Organism=Homo sapiens, GI4557797, Length=154, Percent_Identity=49.3506493506493, Blast_Score=157, Evalue=4e-39,
Organism=Homo sapiens, GI4826862, Length=146, Percent_Identity=47.945205479452, Blast_Score=144, Evalue=4e-35,
Organism=Homo sapiens, GI37693993, Length=150, Percent_Identity=46, Blast_Score=143, Evalue=5e-35,
Organism=Homo sapiens, GI37574614, Length=136, Percent_Identity=34.5588235294118, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI7019465, Length=136, Percent_Identity=34.5588235294118, Blast_Score=81, Evalue=4e-16,
Organism=Homo sapiens, GI5031951, Length=162, Percent_Identity=30.2469135802469, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI4505413, Length=145, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=8e-12,
Organism=Homo sapiens, GI30023828, Length=138, Percent_Identity=29.7101449275362, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788866, Length=139, Percent_Identity=47.4820143884892, Blast_Score=135, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17506807, Length=155, Percent_Identity=46.4516129032258, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322783, Length=153, Percent_Identity=48.3660130718954, Blast_Score=158, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45549037, Length=150, Percent_Identity=50, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI18860097, Length=140, Percent_Identity=33.5714285714286, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDK_PYRFU (Q8U2A8)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578660.1
- ProteinModelPortal:   Q8U2A8
- SMR:   Q8U2A8
- EnsemblBacteria:   EBPYRT00000004168
- GeneID:   1468795
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0931
- NMPDR:   fig|186497.1.peg.966
- GeneTree:   EBGT00050000022477
- HOGENOM:   HBG445152
- OMA:   REIELFF
- ProtClustDB:   PRK00668
- BRENDA:   2.7.4.6
- GO:   GO:0005737
- HAMAP:   MF_00451
- InterPro:   IPR001564
- InterPro:   IPR023005
- Gene3D:   G3DSA:3.30.70.141
- PANTHER:   PTHR11349
- PRINTS:   PR01243
- SMART:   SM00562

Pfam domain/function: PF00334 NDK; SSF54919 NDK

EC number: =2.7.4.6

Molecular weight: Translated: 18392; Mature: 18392

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00469 NDP_KINASES

Important sites: ACT_SITE 121-121 BINDING 12-12 BINDING 60-60 BINDING 88-88 BINDING 94-94 BINDING 105-105 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVERTLVIIKPDAVVRGLIGEIISRFEKKGLKIVGMKMIWIDRELAEKHYEEHKGKPF
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHCCCHH
FEALIDYITKAPVVVMVVEGRYAVEVVRKMAGATDPKDAAPGTIRGDYGLDIGDAIYNVI
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HASDSKESAEREISLYFKPEEIYEYCKAADWFYREKKQAKC
HCCCCHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEVERTLVIIKPDAVVRGLIGEIISRFEKKGLKIVGMKMIWIDRELAEKHYEEHKGKPF
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHCCCHH
FEALIDYITKAPVVVMVVEGRYAVEVVRKMAGATDPKDAAPGTIRGDYGLDIGDAIYNVI
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HASDSKESAEREISLYFKPEEIYEYCKAADWFYREKKQAKC
HCCCCHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA