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The map label for this gene is lacA [C]

Identifier: 18977168

GI number: 18977168

Start: 782274

End: 782774

Strand: Reverse

Name: lacA [C]

Synonym: PF0796

Alternate gene names: 18977168

Gene position: 782774-782274 (Counterclockwise)

Preceding gene: 18977169

Following gene: 18977167

Centisome position: 41.02

GC content: 36.53

Gene sequence:

>501_bases
ATGATGGTGAATACCATGAATATTTTAAGGAAAATATTTCGTAGAATATGTAGAACAGCAGTAAAATTTGTTTTCCCTAA
CGGTTTTAGGATACTGCTATTAAGATGGAGCGGGGTTAAAGTGGGAAAAGATGTGGCAATAAATGAAGGGTTTACGATGG
CTTGTGATATCGGATATGAAGAAAATTTAGTTATTGAAGATAGAGTTGCAATCGGGCCTAATGTTACTATTGTGATAACT
TCTCATCCTAACAATTCACATCTCAGAAATTTAAAAGACAGATATCCTTCAATAGAAGTGTTTGGAAAAGTCCACATAAA
ACATGACGCATGGATAGGGGCGGGAGCCATTATACTCCCAAATGTTACAATAGGAGAGTTCTCAATTATAGGGGCAGGAT
CGGTTGTAACTAAAGACGTTCCGCCATATAGTATAGCGGTTGGAGTCCCGGCTAGGGTAGTAAAGAAACTTAAAATAGAA
AAGAAGACTGAAAATACTTAA

Upstream 100 bases:

>100_bases
TTAAATATAGGCTTGGAACAAAAGTAATTCGGAAAATGCCAATGCCGATTTATAAGAAGTTTAGCGACCGGATATTTGGT
AGTTTAGGGTGAAAACAACT

Downstream 100 bases:

>100_bases
ACGGTGAGAACTGTGAAATTGGAGATAGAGCCATACAAGACATCAAAAATTGTTTTAATGCTTTTAGCAAATAATTTTTA
TATTGATCCAAGAGTTAGAC

Product: galactoside o-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MMVNTMNILRKIFRRICRTAVKFVFPNGFRILLLRWSGVKVGKDVAINEGFTMACDIGYEENLVIEDRVAIGPNVTIVIT
SHPNNSHLRNLKDRYPSIEVFGKVHIKHDAWIGAGAIILPNVTIGEFSIIGAGSVVTKDVPPYSIAVGVPARVVKKLKIE
KKTENT

Sequences:

>Translated_166_residues
MMVNTMNILRKIFRRICRTAVKFVFPNGFRILLLRWSGVKVGKDVAINEGFTMACDIGYEENLVIEDRVAIGPNVTIVIT
SHPNNSHLRNLKDRYPSIEVFGKVHIKHDAWIGAGAIILPNVTIGEFSIIGAGSVVTKDVPPYSIAVGVPARVVKKLKIE
KKTENT
>Mature_166_residues
MMVNTMNILRKIFRRICRTAVKFVFPNGFRILLLRWSGVKVGKDVAINEGFTMACDIGYEENLVIEDRVAIGPNVTIVIT
SHPNNSHLRNLKDRYPSIEVFGKVHIKHDAWIGAGAIILPNVTIGEFSIIGAGSVVTKDVPPYSIAVGVPARVVKKLKIE
KKTENT

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=126, Percent_Identity=38.0952380952381, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1786664, Length=93, Percent_Identity=39.7849462365591, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790035, Length=125, Percent_Identity=36, Blast_Score=61, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=104, Percent_Identity=39.4230769230769, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 18410; Mature: 18410

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVNTMNILRKIFRRICRTAVKFVFPNGFRILLLRWSGVKVGKDVAINEGFTMACDIGYE
CEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEECCEEEECCCEEEEEECCCC
ENLVIEDRVAIGPNVTIVITSHPNNSHLRNLKDRYPSIEVFGKVHIKHDAWIGAGAIILP
CCEEEECEEEECCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEEEECCEECCCEEEEC
NVTIGEFSIIGAGSVVTKDVPPYSIAVGVPARVVKKLKIEKKTENT
CCEECCEEEEECCCEEECCCCCEEEEECCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMVNTMNILRKIFRRICRTAVKFVFPNGFRILLLRWSGVKVGKDVAINEGFTMACDIGYE
CEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEECCEEEECCCEEEEEECCCC
ENLVIEDRVAIGPNVTIVITSHPNNSHLRNLKDRYPSIEVFGKVHIKHDAWIGAGAIILP
CCEEEECEEEECCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEEEEECCEECCCEEEEC
NVTIGEFSIIGAGSVVTKDVPPYSIAVGVPARVVKKLKIEKKTENT
CCEECCEEEEECCCEEECCCCCEEEEECCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]