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The map label for this gene is wecB [C]

Identifier: 18977166

GI number: 18977166

Start: 779986

End: 781062

Strand: Reverse

Name: wecB [C]

Synonym: PF0794

Alternate gene names: 18977166

Gene position: 781062-779986 (Counterclockwise)

Preceding gene: 18977167

Following gene: 18977165

Centisome position: 40.93

GC content: 36.86

Gene sequence:

>1077_bases
ATGAAAATCGCTACTATTGTAGGTGCTCGCCCACAATTCATTAAAATGGCTCCGGTTTCGAGAGAATTAAGAAAGTATTT
TGAAGAAGTTGTAATCCATACTGGACAGCATTATGATTATGAGATGGATAGAATATTTTTTGAACAATTGAATATCCCAG
AGCCAGATTACAACCTTGGTGTTGGTTCTGGAAGCCATGGTTATCAGATTGGAGAGATGCTTAAAAAAATTGAAGAAGTT
TTAATTAAAGAAAAACCAGACCTTGTTATGGTTTTTGGGGACACCAACTCAACTCTTGCTGGAGCTTTAGCTGCGGCTAA
ACTAAAAATTAAAGTTGCTCATGTTGAGGCGGGATTGAGAAGTTTTGATAAATCTATGCCAGAGGAAATAAACAGAATTT
TGACAGATCATGTTAGCGATTACCTATTCGCCCCAACAGAAACCGCTGTAAAAAACTTGTATAACGAAGGAATACGGAAC
GGTGTTTATCTGACTGGAGACGTTATGCTTGATGCTCTTTTATATAACGTCAAAATTGCTGAAAGATGCTCAGAAATCTT
GAATAAGTTAGGATTAAAACCAAAAGAATTTTGTTTAGCAACTGTTCACAGAGCTGAAAATACGGATAACCTTGAGAGGT
TAAAGAACATAATTGATGCCTTTGTAGAATGTAAAGAGACAATTGTTTTTCCAGTTCATCCGAGAACTGAAAAGGTCCTA
AAAGCTTACGGACTTTATGATTCACTCAAAAAAGCTGAAAATGTAATTTTGATTAAACCCGTTGGTTATTTAGACATGCT
AATGTTAGAAAAGAACGCAAAAAAGATACTGACTGATAGTGGTGGTGTGCAGAAAGAAGCGTATTTTTTAAAGGTACCTT
GCATAACTTTGAGAGAAAGAACTGAGTGGGTAGAGACGGTTGAAGATGGTTGGAACGTGCTTGTTGGGGCAGATAAAGAG
AAAATACTTAGAGCTGTTAGAGAATTTGAACCATCGGGAGAAACTTACACCTACAAGTTTGGCGATGGGAGAGCTTCGGA
AAAAATTGCGGAGGTGATAAAGAACGTTGATAGATAA

Upstream 100 bases:

>100_bases
AAAATAGAGGTAATCGTGATAGCAAAAAGAGGGCAAAGTTTTTATGGGAATCGCAAGAAGGGGAGTTTTTAAAGATTTAT
GAAAATCTGGGGGGATTATT

Downstream 100 bases:

>100_bases
AAAGAGAGTTCTTATAATAGCCCCCCACTACAATTCTTTTATAAAAGATTTTACAGAAGCAATGGCTAAGGAAGTTGGAA
GAATTTATGTTTTAGTCCGT

Product: UDP-n-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKIATIVGARPQFIKMAPVSRELRKYFEEVVIHTGQHYDYEMDRIFFEQLNIPEPDYNLGVGSGSHGYQIGEMLKKIEEV
LIKEKPDLVMVFGDTNSTLAGALAAAKLKIKVAHVEAGLRSFDKSMPEEINRILTDHVSDYLFAPTETAVKNLYNEGIRN
GVYLTGDVMLDALLYNVKIAERCSEILNKLGLKPKEFCLATVHRAENTDNLERLKNIIDAFVECKETIVFPVHPRTEKVL
KAYGLYDSLKKAENVILIKPVGYLDMLMLEKNAKKILTDSGGVQKEAYFLKVPCITLRERTEWVETVEDGWNVLVGADKE
KILRAVREFEPSGETYTYKFGDGRASEKIAEVIKNVDR

Sequences:

>Translated_358_residues
MKIATIVGARPQFIKMAPVSRELRKYFEEVVIHTGQHYDYEMDRIFFEQLNIPEPDYNLGVGSGSHGYQIGEMLKKIEEV
LIKEKPDLVMVFGDTNSTLAGALAAAKLKIKVAHVEAGLRSFDKSMPEEINRILTDHVSDYLFAPTETAVKNLYNEGIRN
GVYLTGDVMLDALLYNVKIAERCSEILNKLGLKPKEFCLATVHRAENTDNLERLKNIIDAFVECKETIVFPVHPRTEKVL
KAYGLYDSLKKAENVILIKPVGYLDMLMLEKNAKKILTDSGGVQKEAYFLKVPCITLRERTEWVETVEDGWNVLVGADKE
KILRAVREFEPSGETYTYKFGDGRASEKIAEVIKNVDR
>Mature_358_residues
MKIATIVGARPQFIKMAPVSRELRKYFEEVVIHTGQHYDYEMDRIFFEQLNIPEPDYNLGVGSGSHGYQIGEMLKKIEEV
LIKEKPDLVMVFGDTNSTLAGALAAAKLKIKVAHVEAGLRSFDKSMPEEINRILTDHVSDYLFAPTETAVKNLYNEGIRN
GVYLTGDVMLDALLYNVKIAERCSEILNKLGLKPKEFCLATVHRAENTDNLERLKNIIDAFVECKETIVFPVHPRTEKVL
KAYGLYDSLKKAENVILIKPVGYLDMLMLEKNAKKILTDSGGVQKEAYFLKVPCITLRERTEWVETVEDGWNVLVGADKE
KILRAVREFEPSGETYTYKFGDGRASEKIAEVIKNVDR

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=382, Percent_Identity=35.8638743455497, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 40570; Mature: 40570

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATIVGARPQFIKMAPVSRELRKYFEEVVIHTGQHYDYEMDRIFFEQLNIPEPDYNLG
CEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCC
VGSGSHGYQIGEMLKKIEEVLIKEKPDLVMVFGDTNSTLAGALAAAKLKIKVAHVEAGLR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHEEEEEHHHHHHHH
SFDKSMPEEINRILTDHVSDYLFAPTETAVKNLYNEGIRNGVYLTGDVMLDALLYNVKIA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
ERCSEILNKLGLKPKEFCLATVHRAENTDNLERLKNIIDAFVECKETIVFPVHPRTEKVL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHH
KAYGLYDSLKKAENVILIKPVGYLDMLMLEKNAKKILTDSGGVQKEAYFLKVPCITLRER
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCHHHHHCCCCCCCCCEEEEECCCHHHHHH
TEWVETVEDGWNVLVGADKEKILRAVREFEPSGETYTYKFGDGRASEKIAEVIKNVDR
HHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIATIVGARPQFIKMAPVSRELRKYFEEVVIHTGQHYDYEMDRIFFEQLNIPEPDYNLG
CEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCC
VGSGSHGYQIGEMLKKIEEVLIKEKPDLVMVFGDTNSTLAGALAAAKLKIKVAHVEAGLR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHEEEEEHHHHHHHH
SFDKSMPEEINRILTDHVSDYLFAPTETAVKNLYNEGIRNGVYLTGDVMLDALLYNVKIA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
ERCSEILNKLGLKPKEFCLATVHRAENTDNLERLKNIIDAFVECKETIVFPVHPRTEKVL
HHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHH
KAYGLYDSLKKAENVILIKPVGYLDMLMLEKNAKKILTDSGGVQKEAYFLKVPCITLRER
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCHHHHHCCCCCCCCCEEEEECCCHHHHHH
TEWVETVEDGWNVLVGADKEKILRAVREFEPSGETYTYKFGDGRASEKIAEVIKNVDR
HHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA