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The map label for this gene is pqqE [H]

Identifier: 18977019

GI number: 18977019

Start: 659796

End: 661442

Strand: Reverse

Name: pqqE [H]

Synonym: PF0647

Alternate gene names: 18977019

Gene position: 661442-659796 (Counterclockwise)

Preceding gene: 18977022

Following gene: 18977018

Centisome position: 34.66

GC content: 43.47

Gene sequence:

>1647_bases
ATGACAGAGGATTTTTTAAGTGAGGCTTTGGAGGAACCATTTCCGAAAGCAATGAACACACCACCCTCAAAGGGAGGAAA
TCAAATAACAACAGCCCTTAGAGCTTTCAGATTAATTCTTGGAAATCCACTGGCTAGAGCCTTGCTAAAACCAATGCTCA
AGAAGTATGAAATAAATGGGAGAGAATTGCCAGCCCTTTATTGGGCACTCAGCGTATATGCAGGGGAATCAATAAATTGT
CCCCTCATGATAAGATTCCAAGCTGATATTCTTAAGCTTCTCCTGAAGCTAGGAATAAAACTTGCGAGAGGAGATGAAAA
AGCAGTAAAAGAGGCTCTCCTCAAGGATCCCCATATAAGGAGAGGAATATGGGTGGTATTGGAAGGGATAGCAAAGTATG
GAGTTACAGTCCCCCAAAAGTTAGCTGGGCCTTTCCTCATAGTATGGAACTTCACTAACATGTGTAACTTCCGTTGTAAG
CACTGTTATCAAAGGGCTGACAGGCCATTGTCAAGTGAGCTTACCCTAGAAGAGAAGCTCATGCTAGTTGATCAGTTAGA
CAAAGCTGGAGTTGCTGCTGTGGCATTAAGTGGGGGAGAGCCGACGATACATCCTCACTTCCTAAGAATAGTCAAGGAGC
TTTCAAGTAGGGGGATACATACATCAGTAGCTACTAACGGATGGGTCTTTGCTGATAAGGAGAAGCTTGAAGAAGCTATA
AAGGCTGGCCTTAAATACGTTGAGGTCAGTGTAGATTCAGCAAAACCAGAGAAACATGACGAGTTCAGAGGGATCCCAGG
AGCCTGGGAAAGGGCAGTTAAGGCATTAGAGAATGCTGTTGAGCTTGGCATAAGTCATGGAATGGCTGTAATAATGGACA
AGGATACATATCAAGAGATTGATGACATTCTAGACCTAGCCGAAAACATTGGAGTAAAGAGGGTGATATTCTTTAACTTA
GTTCCAACCGGAAGAGCTGAGGATATGGTAAAGGTCGATTTAACACCAGAAGAAAGAGAAGAGTTCATGAAGGAACTTTA
TCGTCAAATGAAGAAAAGAAAGCTCGAAATACTAACTACTGCTCCTCAATATGCAAGGGTAACTCTCCTTGAGTCCCAGG
GAAAGAGCGTTACTCCTGCTCACTTTTATATAGGAGAGAATAGTGCAGTGAAAACCTTGGCCGAGTTCATTGGAGGTTGT
GGGGCAGGAAGGATATATGCGGGGATAGAGCCAGATGGAACCGTTGTTCCTTGCGTGTTCCTTCCATTGCCGGTGGGAAA
TGTGAGGGTCAAGCCCTTTAAGGAGATATGGGAGAATAGTAGGATATTCAACTTGTTAAGGAACAGGGATAACTTTACGG
GACAGTGTAAAAGGTGCCCCTATAGGAACATTTGCGGGGGTTGTAGAGCTAGAGCTTATCACTACACGCTTGACCTCCTG
GGAGATGATCCTGGGTGCATAATAAATAGGAGGGTGTGGGAGGAGATAGTTAAGCATGGAAAGCCGAGGAGCTTGAGTGA
AGTAAACTGGGTAGATGAGAGCATTGTTCTTCGCGTGCCAATACTCAACATTCCGAAGTATTACAACGTTGTTGAAGAAG
TAGGTGAAAAATTACTTAGTAAAGAAAGTCAAAAAATTCATGCATAA

Upstream 100 bases:

>100_bases
TAAATCTTTCGGACGTATTAGATATGTCAGAAATTTTTGATATATCTTAACCGTAAAGTTTATTATAAAAGACGGACAAG
TCAGAAAAGGGGGATGAATA

Downstream 100 bases:

>100_bases
AAGAGTAAAAATTGTTGATTTTTTACTATTTTTGTTCTTTTTGCAAAGACAATTTTGAAAAGTAACAATGTGAATTTTAA
TACTACTTTCTCTCGTATTT

Product: heme biosynthesis protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTEDFLSEALEEPFPKAMNTPPSKGGNQITTALRAFRLILGNPLARALLKPMLKKYEINGRELPALYWALSVYAGESINC
PLMIRFQADILKLLLKLGIKLARGDEKAVKEALLKDPHIRRGIWVVLEGIAKYGVTVPQKLAGPFLIVWNFTNMCNFRCK
HCYQRADRPLSSELTLEEKLMLVDQLDKAGVAAVALSGGEPTIHPHFLRIVKELSSRGIHTSVATNGWVFADKEKLEEAI
KAGLKYVEVSVDSAKPEKHDEFRGIPGAWERAVKALENAVELGISHGMAVIMDKDTYQEIDDILDLAENIGVKRVIFFNL
VPTGRAEDMVKVDLTPEEREEFMKELYRQMKKRKLEILTTAPQYARVTLLESQGKSVTPAHFYIGENSAVKTLAEFIGGC
GAGRIYAGIEPDGTVVPCVFLPLPVGNVRVKPFKEIWENSRIFNLLRNRDNFTGQCKRCPYRNICGGCRARAYHYTLDLL
GDDPGCIINRRVWEEIVKHGKPRSLSEVNWVDESIVLRVPILNIPKYYNVVEEVGEKLLSKESQKIHA

Sequences:

>Translated_548_residues
MTEDFLSEALEEPFPKAMNTPPSKGGNQITTALRAFRLILGNPLARALLKPMLKKYEINGRELPALYWALSVYAGESINC
PLMIRFQADILKLLLKLGIKLARGDEKAVKEALLKDPHIRRGIWVVLEGIAKYGVTVPQKLAGPFLIVWNFTNMCNFRCK
HCYQRADRPLSSELTLEEKLMLVDQLDKAGVAAVALSGGEPTIHPHFLRIVKELSSRGIHTSVATNGWVFADKEKLEEAI
KAGLKYVEVSVDSAKPEKHDEFRGIPGAWERAVKALENAVELGISHGMAVIMDKDTYQEIDDILDLAENIGVKRVIFFNL
VPTGRAEDMVKVDLTPEEREEFMKELYRQMKKRKLEILTTAPQYARVTLLESQGKSVTPAHFYIGENSAVKTLAEFIGGC
GAGRIYAGIEPDGTVVPCVFLPLPVGNVRVKPFKEIWENSRIFNLLRNRDNFTGQCKRCPYRNICGGCRARAYHYTLDLL
GDDPGCIINRRVWEEIVKHGKPRSLSEVNWVDESIVLRVPILNIPKYYNVVEEVGEKLLSKESQKIHA
>Mature_547_residues
TEDFLSEALEEPFPKAMNTPPSKGGNQITTALRAFRLILGNPLARALLKPMLKKYEINGRELPALYWALSVYAGESINCP
LMIRFQADILKLLLKLGIKLARGDEKAVKEALLKDPHIRRGIWVVLEGIAKYGVTVPQKLAGPFLIVWNFTNMCNFRCKH
CYQRADRPLSSELTLEEKLMLVDQLDKAGVAAVALSGGEPTIHPHFLRIVKELSSRGIHTSVATNGWVFADKEKLEEAIK
AGLKYVEVSVDSAKPEKHDEFRGIPGAWERAVKALENAVELGISHGMAVIMDKDTYQEIDDILDLAENIGVKRVIFFNLV
PTGRAEDMVKVDLTPEEREEFMKELYRQMKKRKLEILTTAPQYARVTLLESQGKSVTPAHFYIGENSAVKTLAEFIGGCG
AGRIYAGIEPDGTVVPCVFLPLPVGNVRVKPFKEIWENSRIFNLLRNRDNFTGQCKRCPYRNICGGCRARAYHYTLDLLG
DDPGCIINRRVWEEIVKHGKPRSLSEVNWVDESIVLRVPILNIPKYYNVVEEVGEKLLSKESQKIHA

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=279, Percent_Identity=27.5985663082437, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI116734668, Length=279, Percent_Identity=27.5985663082437, Blast_Score=73, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574488, Length=179, Percent_Identity=28.4916201117318, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 61634; Mature: 61503

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDFLSEALEEPFPKAMNTPPSKGGNQITTALRAFRLILGNPLARALLKPMLKKYEING
CCHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
RELPALYWALSVYAGESINCPLMIRFQADILKLLLKLGIKLARGDEKAVKEALLKDPHIR
CCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCCHH
RGIWVVLEGIAKYGVTVPQKLAGPFLIVWNFTNMCNFRCKHCYQRADRPLSSELTLEEKL
HHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
MLVDQLDKAGVAAVALSGGEPTIHPHFLRIVKELSSRGIHTSVATNGWVFADKEKLEEAI
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEEECHHHHHHHH
KAGLKYVEVSVDSAKPEKHDEFRGIPGAWERAVKALENAVELGISHGMAVIMDKDTYQEI
HCCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
DDILDLAENIGVKRVIFFNLVPTGRAEDMVKVDLTPEEREEFMKELYRQMKKRKLEILTT
HHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEC
APQYARVTLLESQGKSVTPAHFYIGENSAVKTLAEFIGGCGAGRIYAGIEPDGTVVPCVF
CCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEE
LPLPVGNVRVKPFKEIWENSRIFNLLRNRDNFTGQCKRCPYRNICGGCRARAYHYTLDLL
EECCCCCEECCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHCCEEEEEEEEE
GDDPGCIINRRVWEEIVKHGKPRSLSEVNWVDESIVLRVPILNIPKYYNVVEEVGEKLLS
CCCCCCEECHHHHHHHHHCCCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
KESQKIHA
CHHHHCCC
>Mature Secondary Structure 
TEDFLSEALEEPFPKAMNTPPSKGGNQITTALRAFRLILGNPLARALLKPMLKKYEING
CHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
RELPALYWALSVYAGESINCPLMIRFQADILKLLLKLGIKLARGDEKAVKEALLKDPHIR
CCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCCHH
RGIWVVLEGIAKYGVTVPQKLAGPFLIVWNFTNMCNFRCKHCYQRADRPLSSELTLEEKL
HHHHHHHHHHHHHCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
MLVDQLDKAGVAAVALSGGEPTIHPHFLRIVKELSSRGIHTSVATNGWVFADKEKLEEAI
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEEECHHHHHHHH
KAGLKYVEVSVDSAKPEKHDEFRGIPGAWERAVKALENAVELGISHGMAVIMDKDTYQEI
HCCCEEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
DDILDLAENIGVKRVIFFNLVPTGRAEDMVKVDLTPEEREEFMKELYRQMKKRKLEILTT
HHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEC
APQYARVTLLESQGKSVTPAHFYIGENSAVKTLAEFIGGCGAGRIYAGIEPDGTVVPCVF
CCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEE
LPLPVGNVRVKPFKEIWENSRIFNLLRNRDNFTGQCKRCPYRNICGGCRARAYHYTLDLL
EECCCCCEECCCHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHCCEEEEEEEEE
GDDPGCIINRRVWEEIVKHGKPRSLSEVNWVDESIVLRVPILNIPKYYNVVEEVGEKLLS
CCCCCCEECHHHHHHHHHCCCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHC
KESQKIHA
CHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]