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The map label for this gene is cobS

Identifier: 18976671

GI number: 18976671

Start: 315361

End: 316047

Strand: Direct

Name: cobS

Synonym: PF0299

Alternate gene names: 18976671

Gene position: 315361-316047 (Clockwise)

Preceding gene: 33359466

Following gene: 18976672

Centisome position: 16.53

GC content: 43.09

Gene sequence:

>687_bases
ATGAAGAATTTAATACAATTTATGACGAGGGTTCCGATTAAAGGTGATTTCGAGAAGGCTAGAGAAGAGGTCTGGATGCT
TCCCTTGTTAACACCCCTCACAGCTTTTATTCCGTCGTTGATCCTTTATCTTAACATTCCTCTGAAAAATGTTCTCTCGA
TTCTCTCCCTTTATTGGGTCATTGGTCTACTTCATTTAGATGGGCTTGCTGATTGGGCTGATGGGATTATGGTTAAGGGG
GATAGGGAGAAAAAGGTTAGGGCTATGAAAGACGTCAATACTGGTATTGCTGGAACTTTTGCAGTTGTGATGATCCTCTT
GATTCAGGTTTACTCTCTCTTCTCTGCGCCATTTTACTCCATTTATCTTGCTGAGTTAAATTCTAAGATGGCCATGCTCT
TAGCCTTAGCAACTAAGAAGCCTCTTGGAGAAGGATTGGGAAAGTACTTTATGGATAAGTTGACAACAAAAAGGGTCTTC
CTAGGAGGGGTTCTATACGCTCTCCTCTTGATTCCAATTTTGCTCTATGATCCGCAATCAATATTTGCCCTCCTGGGCTT
AGTAGGGGGAATTTATGCAGTAAAGATAAGCTTGGACAACTTTGGAGGACTAAATGGTGATTGCATAGGGGCCGTGGGGG
AGATAACTAGGGGCGCAACGCTCTTAATCCTGGGGGTTTGGGCATGA

Upstream 100 bases:

>100_bases
AAGGAGGAAGCTCTGCCTCCTGATGTCGTGGAAATACTCAGCAGAGAAGTTGAAATAAATTTGGGTTGGATATTTAAACT
CTTCAGGCATATCCCTAATC

Downstream 100 bases:

>100_bases
TAATCATCATGGCTGGCGGGAAGTCCAGTAGGATGGGAAGGGAAAAGCCCGTTCTTAAGATAGCTGGAAAGGAGATGCTT
CTCTGGGTTTATGAGCAGGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKNLIQFMTRVPIKGDFEKAREEVWMLPLLTPLTAFIPSLILYLNIPLKNVLSILSLYWVIGLLHLDGLADWADGIMVKG
DREKKVRAMKDVNTGIAGTFAVVMILLIQVYSLFSAPFYSIYLAELNSKMAMLLALATKKPLGEGLGKYFMDKLTTKRVF
LGGVLYALLLIPILLYDPQSIFALLGLVGGIYAVKISLDNFGGLNGDCIGAVGEITRGATLLILGVWA

Sequences:

>Translated_228_residues
MKNLIQFMTRVPIKGDFEKAREEVWMLPLLTPLTAFIPSLILYLNIPLKNVLSILSLYWVIGLLHLDGLADWADGIMVKG
DREKKVRAMKDVNTGIAGTFAVVMILLIQVYSLFSAPFYSIYLAELNSKMAMLLALATKKPLGEGLGKYFMDKLTTKRVF
LGGVLYALLLIPILLYDPQSIFALLGLVGGIYAVKISLDNFGGLNGDCIGAVGEITRGATLLILGVWA
>Mature_228_residues
MKNLIQFMTRVPIKGDFEKAREEVWMLPLLTPLTAFIPSLILYLNIPLKNVLSILSLYWVIGLLHLDGLADWADGIMVKG
DREKKVRAMKDVNTGIAGTFAVVMILLIQVYSLFSAPFYSIYLAELNSKMAMLLALATKKPLGEGLGKYFMDKLTTKRVF
LGGVLYALLLIPILLYDPQSIFALLGLVGGIYAVKISLDNFGGLNGDCIGAVGEITRGATLLILGVWA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=234, Percent_Identity=28.6324786324786, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_PYRFU (Q8U400)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578028.1
- ProteinModelPortal:   Q8U400
- EnsemblBacteria:   EBPYRT00000005549
- GeneID:   1468134
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0299
- NMPDR:   fig|186497.1.peg.305
- GeneTree:   EBGT00050000023326
- HOGENOM:   HBG729392
- OMA:   GLHHLDG
- ProtClustDB:   PRK00235
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 25028; Mature: 25028

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc45fa74)-; HASH(0x4039fdf8)-; HASH(0xb472894)-; HASH(0xc29c57c)-; HASH(0xca2fda4)-; HASH(0xc45ff78)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLIQFMTRVPIKGDFEKAREEVWMLPLLTPLTAFIPSLILYLNIPLKNVLSILSLYWV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IGLLHLDGLADWADGIMVKGDREKKVRAMKDVNTGIAGTFAVVMILLIQVYSLFSAPFYS
HHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHH
IYLAELNSKMAMLLALATKKPLGEGLGKYFMDKLTTKRVFLGGVLYALLLIPILLYDPQS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IFALLGLVGGIYAVKISLDNFGGLNGDCIGAVGEITRGATLLILGVWA
HHHHHHHHCCHHEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECH
>Mature Secondary Structure
MKNLIQFMTRVPIKGDFEKAREEVWMLPLLTPLTAFIPSLILYLNIPLKNVLSILSLYWV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IGLLHLDGLADWADGIMVKGDREKKVRAMKDVNTGIAGTFAVVMILLIQVYSLFSAPFYS
HHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHH
IYLAELNSKMAMLLALATKKPLGEGLGKYFMDKLTTKRVFLGGVLYALLLIPILLYDPQS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IFALLGLVGGIYAVKISLDNFGGLNGDCIGAVGEITRGATLLILGVWA
HHHHHHHHCCHHEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA