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The map label for this gene is napA [H]

Identifier: 18976647

GI number: 18976647

Start: 285783

End: 286922

Strand: Direct

Name: napA [H]

Synonym: PF0275

Alternate gene names: 18976647

Gene position: 285783-286922 (Clockwise)

Preceding gene: 18976646

Following gene: 18976654

Centisome position: 14.98

GC content: 40.61

Gene sequence:

>1140_bases
ATGGATGTATTTTTAGAATTAGCTCTGATATTAATAATGGCTAAAATCTTCGGCTATATTTCGGTGAGATTTGGATTTCC
AGCTGCCTTAGGACAGCTCATAGGGGGGATTATAATAGGTCCCTCTCTCCTCAACATAGTTCCTTACAGTGAAGAGGTTA
GGTTGCTTGCTGAGCTTGGGGTTGTAATCCTTCTCTTTTTGGCTGGACTTGAGACTGATGTCGAGGAATTCAAGAAGGTA
GGTTTTCCGGCCTTTATAGTTGCCATTCTGGGAGTCTTTGTACCTTTTGTTCTGGGATATGCTTCTGCACTTGCCTTTGG
ATATCCAAACATGCAGGCTCTTTTTCTTGGAGGAGTGCTTACAGCTACAAGCGTTGGCCTTACAACGAGCATATTAATGG
AGATGAAGAAGCTAAGAACGAAGGTTGGGACAACTATCTTAGCAGCTGCTGTGATAGATGATGTGCTTGGCATAATTGTT
CTCACTATTTTAGTTGCAATAAGTACGAGGGGTAGCGTCAATTTGTTTGATTTAGGAATAATCTTTCTTGAAGTAGTAGT
GTTCTTTACCCTTGGCCTACTCTTAGGTAATCCAGCTGTTAAAGAAATGCTCAAGTTATCAGAAAGGATAACACTTCCTG
AAACTGTAACGGCTTTTGCAATTTCTATAATGCTACTCTTCGCATACATAGCTGAAAAGTTTCAATTGGCCGGGATTACA
GGCTCTTATTTGGCAGGAATACTTGTTGCAATGACTGAGGAGGCAAGAGAAATAACAAACAAAATAATGACAATTGGATA
TTCTCTTTTTATTCCCATCTTCCTTGTGAGCATTGGAATAGAGAGTGATCTTCATGTTTTGCTCCATGCTAGTCTTTTTG
CAATTGTATATGCTGTTGTTGGTATAGTGGGCAAGGTTGTGGGGTGTTCGCTTGGAGCTCTTCTCGTAAAGTTCAAGCCG
AAGGAAGCACTTCAAGTTGGGGTTGGTATGATACCAAGGATGGAAGTTGCCTTAATTATGGCAAATATTGGATTAAGGGA
GGGGGTATTTACGAGAGATGTATTTTCAATTCCTGTCGTCATGGTTGTTGTTACGACTTTAGTTACTCCATTCCTTCTAA
AGCTGGCGTTTTCGAAGTAG

Upstream 100 bases:

>100_bases
TTTCATTCTCGGTTTGGTTGTCCGCCTGGTGATGTGACTGAGTGGCTTCAAGAGGTGATGCCCCAGTTATCCTGACAGTA
TCTAGCAAGGGGATGATAAC

Downstream 100 bases:

>100_bases
CTACTCTTCTGGTTCTAATGATTCTATCTTTTCCTTCCAGTTTCCAGGCTTTTTAAAGTCTTTTAAGTTGTATAGGCCTT
CCTTTACTAGTATTCCCCTT

Product: Na+/H+ antiporter

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MDVFLELALILIMAKIFGYISVRFGFPAALGQLIGGIIIGPSLLNIVPYSEEVRLLAELGVVILLFLAGLETDVEEFKKV
GFPAFIVAILGVFVPFVLGYASALAFGYPNMQALFLGGVLTATSVGLTTSILMEMKKLRTKVGTTILAAAVIDDVLGIIV
LTILVAISTRGSVNLFDLGIIFLEVVVFFTLGLLLGNPAVKEMLKLSERITLPETVTAFAISIMLLFAYIAEKFQLAGIT
GSYLAGILVAMTEEAREITNKIMTIGYSLFIPIFLVSIGIESDLHVLLHASLFAIVYAVVGIVGKVVGCSLGALLVKFKP
KEALQVGVGMIPRMEVALIMANIGLREGVFTRDVFSIPVVMVVVTTLVTPFLLKLAFSK

Sequences:

>Translated_379_residues
MDVFLELALILIMAKIFGYISVRFGFPAALGQLIGGIIIGPSLLNIVPYSEEVRLLAELGVVILLFLAGLETDVEEFKKV
GFPAFIVAILGVFVPFVLGYASALAFGYPNMQALFLGGVLTATSVGLTTSILMEMKKLRTKVGTTILAAAVIDDVLGIIV
LTILVAISTRGSVNLFDLGIIFLEVVVFFTLGLLLGNPAVKEMLKLSERITLPETVTAFAISIMLLFAYIAEKFQLAGIT
GSYLAGILVAMTEEAREITNKIMTIGYSLFIPIFLVSIGIESDLHVLLHASLFAIVYAVVGIVGKVVGCSLGALLVKFKP
KEALQVGVGMIPRMEVALIMANIGLREGVFTRDVFSIPVVMVVVTTLVTPFLLKLAFSK
>Mature_379_residues
MDVFLELALILIMAKIFGYISVRFGFPAALGQLIGGIIIGPSLLNIVPYSEEVRLLAELGVVILLFLAGLETDVEEFKKV
GFPAFIVAILGVFVPFVLGYASALAFGYPNMQALFLGGVLTATSVGLTTSILMEMKKLRTKVGTTILAAAVIDDVLGIIV
LTILVAISTRGSVNLFDLGIIFLEVVVFFTLGLLLGNPAVKEMLKLSERITLPETVTAFAISIMLLFAYIAEKFQLAGIT
GSYLAGILVAMTEEAREITNKIMTIGYSLFIPIFLVSIGIESDLHVLLHASLFAIVYAVVGIVGKVVGCSLGALLVKFKP
KEALQVGVGMIPRMEVALIMANIGLREGVFTRDVFSIPVVMVVVTTLVTPFLLKLAFSK

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=274, Percent_Identity=27.3722627737226, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789749, Length=358, Percent_Identity=24.8603351955307, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322367, Length=382, Percent_Identity=27.7486910994764, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 40596; Mature: 40596

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFLELALILIMAKIFGYISVRFGFPAALGQLIGGIIIGPSLLNIVPYSEEVRLLAELG
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHH
VVILLFLAGLETDVEEFKKVGFPAFIVAILGVFVPFVLGYASALAFGYPNMQALFLGGVL
HHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TATSVGLTTSILMEMKKLRTKVGTTILAAAVIDDVLGIIVLTILVAISTRGSVNLFDLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IFLEVVVFFTLGLLLGNPAVKEMLKLSERITLPETVTAFAISIMLLFAYIAEKFQLAGIT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSYLAGILVAMTEEAREITNKIMTIGYSLFIPIFLVSIGIESDLHVLLHASLFAIVYAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GIVGKVVGCSLGALLVKFKPKEALQVGVGMIPRMEVALIMANIGLREGVFTRDVFSIPVV
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
MVVVTTLVTPFLLKLAFSK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVFLELALILIMAKIFGYISVRFGFPAALGQLIGGIIIGPSLLNIVPYSEEVRLLAELG
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHH
VVILLFLAGLETDVEEFKKVGFPAFIVAILGVFVPFVLGYASALAFGYPNMQALFLGGVL
HHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TATSVGLTTSILMEMKKLRTKVGTTILAAAVIDDVLGIIVLTILVAISTRGSVNLFDLGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IFLEVVVFFTLGLLLGNPAVKEMLKLSERITLPETVTAFAISIMLLFAYIAEKFQLAGIT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSYLAGILVAMTEEAREITNKIMTIGYSLFIPIFLVSIGIESDLHVLLHASLFAIVYAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GIVGKVVGCSLGALLVKFKPKEALQVGVGMIPRMEVALIMANIGLREGVFTRDVFSIPVV
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
MVVVTTLVTPFLLKLAFSK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]