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The map label for this gene is amyA [H]

Identifier: 18976644

GI number: 18976644

Start: 281958

End: 283928

Strand: Direct

Name: amyA [H]

Synonym: PF0272

Alternate gene names: 18976644

Gene position: 281958-283928 (Clockwise)

Preceding gene: 18976642

Following gene: 18976646

Centisome position: 14.78

GC content: 41.6

Gene sequence:

>1971_bases
GTGATTAATGGGTGGACGGAAGTGGGAGATAAAATTAACTTCATATTTGGAATTCACAACCATCAGCCCCTGGGCAACTT
TGGATGGGTGTTTGAGGAGGCTTATGAAAAGTGTTACTGGCCGTTTCTGGAGACTCTGGAGGAATATCCAAACATGAAGG
TTGCCATTCATACAAGTGGCCCCCTCATTGAGTGGCTCCAAGATAATAGACCCGAATACATAGACTTGCTTAGAAGTCTA
GTGAAAAGAGGACAGGTGGAGATAGTCGTTGCTGGGTTCTACGAGCCTGTGCTAGCATCAATCCCAAAGGAAGATAGAAT
AGAGCAGATAAGGTTAATGAAAGAGTGGGCTAAGAGTATTGGATTTGATGCTAGGGGAGTTTGGCTAACTGAAAGAGTAT
GGCAACCAGAGCTCGTAAAGACCCTTAAGGAGAGCGGAATAGATTATGTAATAGTTGACGATTACCACTTCATGAGTGCG
GGATTAAGTAAAGAGGAGCTGTACTGGCCATATTATACGGAAGATGGTGGGGAAGTTATAGCTGTTTTCCCGATAGATGA
GAAGTTGAGATATTTGATTCCCTTTAGACCCGTTGATAAGGTCTTAGAATACCTGCATTCTCTCATAGATGGTGATGAGA
GCAAAGTTGCAGTATTTCATGACGATGGTGAGAAGTTTGGAATCTGGCCTGGAACTTATGAGTGGGTGTATGAAAAGGGA
TGGTTAAGAGAATTCTTTGATAGAATTTCAAGTGATGAAAAGATAAACTTAATGCTTTACACTGAATACTTAGAAAAATA
TAAGCCTAGAGGTCTTGTTTATCTTCCAATAGCTTCATATTTTGAGATGAGCGAATGGTCATTGCCAGCAAAGCAGGCAA
GGCTCTTTGTGGAGTTCGTCAATGAGCTTAAAGTTAAAGGTATATTTGAAAAGTACAGGGTATTTGTTAGGGGAGGAATT
TGGAAGAATTTCTTCTATAAATACCCAGAGAGCAACTACATGCACAAGAGAATGCTAATGGTAAGTAAGTTAGTGAGAAA
CAATCCTGAGGCCAGGAAGTATCTGCTGAGAGCACAATGTAACGATGCTTATTGGCACGGCCTCTTCGGTGGAGTATATT
TACCCCATCTTAGGAGGGCCATCTGGAACAATTTAATCAAGGCCAACAGCTATGTAAGCCTTGGAAAGGTCATAAGGGAT
ATCGACTACGATGGCTTTGAGGAAGTTCTCATAGAGAATGACAACTTTTATGCAGTGTTTAAACCCTCTTACGGTGGTTC
CTTGGTGGAGTTTTCATCAAAGAATAGACTCGTGAATTATGTAGATGTTCTGGCAAGAAGGTGGGAACACTATCATGGCT
ATGTGGAAAGTCAATTTGATGGAGTAGCCAGCATTCATGAGCTCGAGAAAAAGATACCAGATGAAATAAGAAAAGAAGTT
GCTTACGACAAGTACAGAAGGTTCATGCTTCAAGATCACGTAGTCCCCCTGGGAACAACTCTGGAAGACTTCATGTTCTC
AAGACAACAGGAGATCGGAGAGTTTCCTAGGGTTCCATACTCATATGAACTACTAGATGGAGGAATAAGGCTGAAGAGGG
AACACTTGGGAATAGAAGTTGAAAAAACAGTGAAGTTAGTGAATGATGGATTTGAGGTGGAGTATATAGTGAACAACAAG
ACAGGAAATCCTGTATTGTTCGCAGTGGAACTTAACGTTGCAGTTCAGAGCATAATGGAGAGCCCAGGAGTTCTAAGGGG
GAAAGAAATTGTCGTTGATGACAAGTATGCAGTTGGGAAGTTTGCACTGAAGTTTGAAGACGAAATGGAAGTCTGGAAGT
ATCCAGTAAAGACTCTCAGTCAAAGTGAAAGTGGCTGGGATCTAATCCAGCAGGGTGTCAGCTACATAGTTCCAATAAGG
TTGGAGGATAAAATAAGGTTTAAGCTAAAATTTGAGGAAGCCTCGGGATAG

Upstream 100 bases:

>100_bases
CTTGTCCATGCTAACACCCTGTAATGAGATTTGGATTTTCCTATATAAAAAGCCTTAGTTATTTTTGAGCCATTAAATAT
ATAAGGAAGTATCACTCTTA

Downstream 100 bases:

>100_bases
GGAGGCCCTCATCACCAATCAGGGCCCGAAAGACTCCCTCATCGGCCCTTCTATTTTATTTTAAACGTCAATGGTTTACC
AAGTTTCCAAAACTTACAAA

Product: alpha-amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MINGWTEVGDKINFIFGIHNHQPLGNFGWVFEEAYEKCYWPFLETLEEYPNMKVAIHTSGPLIEWLQDNRPEYIDLLRSL
VKRGQVEIVVAGFYEPVLASIPKEDRIEQIRLMKEWAKSIGFDARGVWLTERVWQPELVKTLKESGIDYVIVDDYHFMSA
GLSKEELYWPYYTEDGGEVIAVFPIDEKLRYLIPFRPVDKVLEYLHSLIDGDESKVAVFHDDGEKFGIWPGTYEWVYEKG
WLREFFDRISSDEKINLMLYTEYLEKYKPRGLVYLPIASYFEMSEWSLPAKQARLFVEFVNELKVKGIFEKYRVFVRGGI
WKNFFYKYPESNYMHKRMLMVSKLVRNNPEARKYLLRAQCNDAYWHGLFGGVYLPHLRRAIWNNLIKANSYVSLGKVIRD
IDYDGFEEVLIENDNFYAVFKPSYGGSLVEFSSKNRLVNYVDVLARRWEHYHGYVESQFDGVASIHELEKKIPDEIRKEV
AYDKYRRFMLQDHVVPLGTTLEDFMFSRQQEIGEFPRVPYSYELLDGGIRLKREHLGIEVEKTVKLVNDGFEVEYIVNNK
TGNPVLFAVELNVAVQSIMESPGVLRGKEIVVDDKYAVGKFALKFEDEMEVWKYPVKTLSQSESGWDLIQQGVSYIVPIR
LEDKIRFKLKFEEASG

Sequences:

>Translated_656_residues
MINGWTEVGDKINFIFGIHNHQPLGNFGWVFEEAYEKCYWPFLETLEEYPNMKVAIHTSGPLIEWLQDNRPEYIDLLRSL
VKRGQVEIVVAGFYEPVLASIPKEDRIEQIRLMKEWAKSIGFDARGVWLTERVWQPELVKTLKESGIDYVIVDDYHFMSA
GLSKEELYWPYYTEDGGEVIAVFPIDEKLRYLIPFRPVDKVLEYLHSLIDGDESKVAVFHDDGEKFGIWPGTYEWVYEKG
WLREFFDRISSDEKINLMLYTEYLEKYKPRGLVYLPIASYFEMSEWSLPAKQARLFVEFVNELKVKGIFEKYRVFVRGGI
WKNFFYKYPESNYMHKRMLMVSKLVRNNPEARKYLLRAQCNDAYWHGLFGGVYLPHLRRAIWNNLIKANSYVSLGKVIRD
IDYDGFEEVLIENDNFYAVFKPSYGGSLVEFSSKNRLVNYVDVLARRWEHYHGYVESQFDGVASIHELEKKIPDEIRKEV
AYDKYRRFMLQDHVVPLGTTLEDFMFSRQQEIGEFPRVPYSYELLDGGIRLKREHLGIEVEKTVKLVNDGFEVEYIVNNK
TGNPVLFAVELNVAVQSIMESPGVLRGKEIVVDDKYAVGKFALKFEDEMEVWKYPVKTLSQSESGWDLIQQGVSYIVPIR
LEDKIRFKLKFEEASG
>Mature_656_residues
MINGWTEVGDKINFIFGIHNHQPLGNFGWVFEEAYEKCYWPFLETLEEYPNMKVAIHTSGPLIEWLQDNRPEYIDLLRSL
VKRGQVEIVVAGFYEPVLASIPKEDRIEQIRLMKEWAKSIGFDARGVWLTERVWQPELVKTLKESGIDYVIVDDYHFMSA
GLSKEELYWPYYTEDGGEVIAVFPIDEKLRYLIPFRPVDKVLEYLHSLIDGDESKVAVFHDDGEKFGIWPGTYEWVYEKG
WLREFFDRISSDEKINLMLYTEYLEKYKPRGLVYLPIASYFEMSEWSLPAKQARLFVEFVNELKVKGIFEKYRVFVRGGI
WKNFFYKYPESNYMHKRMLMVSKLVRNNPEARKYLLRAQCNDAYWHGLFGGVYLPHLRRAIWNNLIKANSYVSLGKVIRD
IDYDGFEEVLIENDNFYAVFKPSYGGSLVEFSSKNRLVNYVDVLARRWEHYHGYVESQFDGVASIHELEKKIPDEIRKEV
AYDKYRRFMLQDHVVPLGTTLEDFMFSRQQEIGEFPRVPYSYELLDGGIRLKREHLGIEVEKTVKLVNDGFEVEYIVNNK
TGNPVLFAVELNVAVQSIMESPGVLRGKEIVVDDKYAVGKFALKFEDEMEVWKYPVKTLSQSESGWDLIQQGVSYIVPIR
LEDKIRFKLKFEEASG

Specific function: Displays a broad range of substrate specificity, with the capacity to hydrolyze carbohydrates as simple as maltotriose [H]

COG id: COG1449

COG function: function code G; Alpha-amylase/alpha-mannosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 57 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015179
- InterPro:   IPR015178
- InterPro:   IPR011013
- InterPro:   IPR011330
- InterPro:   IPR000602
- InterPro:   IPR004300
- ProDom:   PD014405 [H]

Pfam domain/function: PF09094 DUF1925; PF09095 DUF1926; PF03065 Glyco_hydro_57 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 77110; Mature: 77110

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINGWTEVGDKINFIFGIHNHQPLGNFGWVFEEAYEKCYWPFLETLEEYPNMKVAIHTSG
CCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PLIEWLQDNRPEYIDLLRSLVKRGQVEIVVAGFYEPVLASIPKEDRIEQIRLMKEWAKSI
CHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHC
GFDARGVWLTERVWQPELVKTLKESGIDYVIVDDYHFMSAGLSKEELYWPYYTEDGGEVI
CCCCCCEEEEHHCCCHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCEECCEEECCCCCEE
AVFPIDEKLRYLIPFRPVDKVLEYLHSLIDGDESKVAVFHDDGEKFGIWPGTYEWVYEKG
EEEECCCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCCHHHHHCCC
WLREFFDRISSDEKINLMLYTEYLEKYKPRGLVYLPIASYFEMSEWSLPAKQARLFVEFV
HHHHHHHHHCCCCEEEEEEEHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHH
NELKVKGIFEKYRVFVRGGIWKNFFYKYPESNYMHKRMLMVSKLVRNNPEARKYLLRAQC
HHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
NDAYWHGLFGGVYLPHLRRAIWNNLIKANSYVSLGKVIRDIDYDGFEEVLIENDNFYAVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHEEECCCEEEEE
KPSYGGSLVEFSSKNRLVNYVDVLARRWEHYHGYVESQFDGVASIHELEKKIPDEIRKEV
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCHHHHHHH
AYDKYRRFMLQDHVVPLGTTLEDFMFSRQQEIGEFPRVPYSYELLDGGIRLKREHLGIEV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHEECCCCEEEHHHCCEEH
EKTVKLVNDGFEVEYIVNNKTGNPVLFAVELNVAVQSIMESPGVLRGKEIVVDDKYAVGK
HHHHHHHCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCEEECE
FALKFEDEMEVWKYPVKTLSQSESGWDLIQQGVSYIVPIRLEDKIRFKLKFEEASG
EEEEECCHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEECCCEEEEEEEECCCC
>Mature Secondary Structure
MINGWTEVGDKINFIFGIHNHQPLGNFGWVFEEAYEKCYWPFLETLEEYPNMKVAIHTSG
CCCCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
PLIEWLQDNRPEYIDLLRSLVKRGQVEIVVAGFYEPVLASIPKEDRIEQIRLMKEWAKSI
CHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHC
GFDARGVWLTERVWQPELVKTLKESGIDYVIVDDYHFMSAGLSKEELYWPYYTEDGGEVI
CCCCCCEEEEHHCCCHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCEECCEEECCCCCEE
AVFPIDEKLRYLIPFRPVDKVLEYLHSLIDGDESKVAVFHDDGEKFGIWPGTYEWVYEKG
EEEECCCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCCCCHHHHHCCC
WLREFFDRISSDEKINLMLYTEYLEKYKPRGLVYLPIASYFEMSEWSLPAKQARLFVEFV
HHHHHHHHHCCCCEEEEEEEHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHH
NELKVKGIFEKYRVFVRGGIWKNFFYKYPESNYMHKRMLMVSKLVRNNPEARKYLLRAQC
HHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
NDAYWHGLFGGVYLPHLRRAIWNNLIKANSYVSLGKVIRDIDYDGFEEVLIENDNFYAVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHEEECCCEEEEE
KPSYGGSLVEFSSKNRLVNYVDVLARRWEHYHGYVESQFDGVASIHELEKKIPDEIRKEV
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCHHHHHHH
AYDKYRRFMLQDHVVPLGTTLEDFMFSRQQEIGEFPRVPYSYELLDGGIRLKREHLGIEV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHEECCCCEEEHHHCCEEH
EKTVKLVNDGFEVEYIVNNKTGNPVLFAVELNVAVQSIMESPGVLRGKEIVVDDKYAVGK
HHHHHHHCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCCCCCCCEEEEECCEEECE
FALKFEDEMEVWKYPVKTLSQSESGWDLIQQGVSYIVPIRLEDKIRFKLKFEEASG
EEEEECCHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEEECCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226990; 8226989 [H]