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The map label for this gene is purF [H]

Identifier: 18976526

GI number: 18976526

Start: 160961

End: 162310

Strand: Direct

Name: purF [H]

Synonym: PF0154

Alternate gene names: 18976526

Gene position: 160961-162310 (Clockwise)

Preceding gene: 18976523

Following gene: 18976532

Centisome position: 8.43

GC content: 40.59

Gene sequence:

>1350_bases
GTGAGGGGTGAAAGCATAGTGATTAGGGAGAAATGTGGTATATTTGCGGCATATGCTGATAATGCTCCTGAGAAGGCTTA
TTACTCTCTGTTATCACTCCAGCATAGAGGTCAGGAGGGAGCTGGAATTACTTTTTGGGAAAATGGTATCAAGACGATAA
AGGGAATTGGGCTTGTCCCTGAAGTTTTTAAAGGGGGAATAGAGTCGAAATCAAGCCTTGCAATTGGACATGTGAGATAT
TCTACTTTTGGCTCCCTTACGGAAGTTCAGCCCATTGAAGTTAAATGTTGCAATTATAAGCTTGCAATAGCTCACAATGG
GACTCTTACAAACTATCTGCCATTAAGAGAGAAGTATGAAAGTGAGGGCTTCAAGTTTAACTCCACTATAGACACAGAGC
TAATTGGAGTGGCCTTTTTGAAACATTACAAAGAGCTCGGAGATGAATTCAAGGCAATGGAAGAAGTTTTTAATGAAGTT
AGGGGAGCTTATTCTGTGGCAATTCTCTTTGATGGAAAGATAATCGTGGCGAGAGATCCCGCTGGTTTTAGACCTTTAGC
TTATGGAGAAGGAGATGGTTTTTATTTCGCTTCTGAGAACTCAGGTTTGAGAATGTTTACAAATAAGATTAGAGATGTCT
TTCCAGGCGAAGTTTTTGTTGTTGAAGAGGGTGAAATTTATAGTAAAGTTCTGAAGAAACTTCCTCATAGCCATTGTGTT
TTTGAATATATTTACTTTGCAAGACCTGATAGCAATATAGAGGGGGTAAGTGTTTACTTGGCAAGGTATAGGATGGGAGT
TGAATTAGCAAGAGAAAGCCCAGCTCCTGGAGATGTTGTTATAGCTGTTCCCGATTCTGGAAGGACAGCGGCCCTTGGTT
TTGCCCATGAAAGTGGAATTCCTTATATGGAAGGACTTATAAAGAACCGTTATATTGGGAGAACTTTTATCATGCCCAGC
GGAAGGGAGCTTAAAGTTAAGCTAAAGCTTTCTCCTGTAAGGGAGGTCATTGAGGGAAAAAGGGTGGTTTTGGTTGATGA
TTCAATAGTTAGAGGAACGACCATAAAGAGAATAGTAAAGATGATTAGAGATGCAGGGGCTGAGGAAGTTCATGTAAGAA
TTTCTTCACCTCCAATTAGATATCCATGCTACATGGGGATAGATATTCCAACTAGGCATGAGCTTATTGCAGCATGGAGA
ACTGTCGAGGATATAAGAAGAGAAATTGGAGCTGATTCTTTAGCTTATTTGAGCATAGAAGGCCTTAAAAGAGCTGTAGG
AATAGAGGATCTTTGCATGGCCTGCTTAACTGGTCATTATCCCGAGTGGGCCTTTGACTTTGAGTTATGA

Upstream 100 bases:

>100_bases
CTTTTTGGCCTTGCCCTCATATATCTCCATTCATTTCACCATTTTTATGTAAAAATCACAATCCTTTTAAGTATTTTTAG
ACAAGAAGACGTCAAAGAAA

Downstream 100 bases:

>100_bases
CCTTATCCAACTATTGGCTCCTGGGTTATTATTAACTTACCCTACGTTTGGATACGAGCTGATGAGGGATCATAGGGATG
GGAAGGAGAACTGCCCTTAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRGESIVIREKCGIFAAYADNAPEKAYYSLLSLQHRGQEGAGITFWENGIKTIKGIGLVPEVFKGGIESKSSLAIGHVRY
STFGSLTEVQPIEVKCCNYKLAIAHNGTLTNYLPLREKYESEGFKFNSTIDTELIGVAFLKHYKELGDEFKAMEEVFNEV
RGAYSVAILFDGKIIVARDPAGFRPLAYGEGDGFYFASENSGLRMFTNKIRDVFPGEVFVVEEGEIYSKVLKKLPHSHCV
FEYIYFARPDSNIEGVSVYLARYRMGVELARESPAPGDVVIAVPDSGRTAALGFAHESGIPYMEGLIKNRYIGRTFIMPS
GRELKVKLKLSPVREVIEGKRVVLVDDSIVRGTTIKRIVKMIRDAGAEEVHVRISSPPIRYPCYMGIDIPTRHELIAAWR
TVEDIRREIGADSLAYLSIEGLKRAVGIEDLCMACLTGHYPEWAFDFEL

Sequences:

>Translated_449_residues
MRGESIVIREKCGIFAAYADNAPEKAYYSLLSLQHRGQEGAGITFWENGIKTIKGIGLVPEVFKGGIESKSSLAIGHVRY
STFGSLTEVQPIEVKCCNYKLAIAHNGTLTNYLPLREKYESEGFKFNSTIDTELIGVAFLKHYKELGDEFKAMEEVFNEV
RGAYSVAILFDGKIIVARDPAGFRPLAYGEGDGFYFASENSGLRMFTNKIRDVFPGEVFVVEEGEIYSKVLKKLPHSHCV
FEYIYFARPDSNIEGVSVYLARYRMGVELARESPAPGDVVIAVPDSGRTAALGFAHESGIPYMEGLIKNRYIGRTFIMPS
GRELKVKLKLSPVREVIEGKRVVLVDDSIVRGTTIKRIVKMIRDAGAEEVHVRISSPPIRYPCYMGIDIPTRHELIAAWR
TVEDIRREIGADSLAYLSIEGLKRAVGIEDLCMACLTGHYPEWAFDFEL
>Mature_449_residues
MRGESIVIREKCGIFAAYADNAPEKAYYSLLSLQHRGQEGAGITFWENGIKTIKGIGLVPEVFKGGIESKSSLAIGHVRY
STFGSLTEVQPIEVKCCNYKLAIAHNGTLTNYLPLREKYESEGFKFNSTIDTELIGVAFLKHYKELGDEFKAMEEVFNEV
RGAYSVAILFDGKIIVARDPAGFRPLAYGEGDGFYFASENSGLRMFTNKIRDVFPGEVFVVEEGEIYSKVLKKLPHSHCV
FEYIYFARPDSNIEGVSVYLARYRMGVELARESPAPGDVVIAVPDSGRTAALGFAHESGIPYMEGLIKNRYIGRTFIMPS
GRELKVKLKLSPVREVIEGKRVVLVDDSIVRGTTIKRIVKMIRDAGAEEVHVRISSPPIRYPCYMGIDIPTRHELIAAWR
TVEDIRREIGADSLAYLSIEGLKRAVGIEDLCMACLTGHYPEWAFDFEL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=38.1044487427466, Blast_Score=311, Evalue=1e-84,
Organism=Escherichia coli, GI1788651, Length=439, Percent_Identity=41.2300683371298, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1790167, Length=227, Percent_Identity=27.7533039647577, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=474, Percent_Identity=38.8185654008439, Blast_Score=293, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323958, Length=450, Percent_Identity=39.3333333333333, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=40.6639004149378, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI28573187, Length=488, Percent_Identity=40.5737704918033, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24659604, Length=456, Percent_Identity=41.6666666666667, Blast_Score=313, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50172; Mature: 50172

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGESIVIREKCGIFAAYADNAPEKAYYSLLSLQHRGQEGAGITFWENGIKTIKGIGLVP
CCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCH
EVFKGGIESKSSLAIGHVRYSTFGSLTEVQPIEVKCCNYKLAIAHNGTLTNYLPLREKYE
HHHCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECEEEEEEECCCCCCCCCHHHHHC
SEGFKFNSTIDTELIGVAFLKHYKELGDEFKAMEEVFNEVRGAYSVAILFDGKIIVARDP
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECC
AGFRPLAYGEGDGFYFASENSGLRMFTNKIRDVFPGEVFVVEEGEIYSKVLKKLPHSHCV
CCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCEE
FEYIYFARPDSNIEGVSVYLARYRMGVELARESPAPGDVVIAVPDSGRTAALGFAHESGI
EEEEEEECCCCCCCHHHHEEHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHCCC
PYMEGLIKNRYIGRTFIMPSGRELKVKLKLSPVREVIEGKRVVLVDDSIVRGTTIKRIVK
HHHHHHHHCCCCCEEEECCCCCEEEEEEEECHHHHHHCCCEEEEECCCCCCCHHHHHHHH
MIRDAGAEEVHVRISSPPIRYPCYMGIDIPTRHELIAAWRTVEDIRREIGADSLAYLSIE
HHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
GLKRAVGIEDLCMACLTGHYPEWAFDFEL
HHHHHCCHHHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MRGESIVIREKCGIFAAYADNAPEKAYYSLLSLQHRGQEGAGITFWENGIKTIKGIGLVP
CCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCH
EVFKGGIESKSSLAIGHVRYSTFGSLTEVQPIEVKCCNYKLAIAHNGTLTNYLPLREKYE
HHHCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECEEEEEEECCCCCCCCCHHHHHC
SEGFKFNSTIDTELIGVAFLKHYKELGDEFKAMEEVFNEVRGAYSVAILFDGKIIVARDP
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECC
AGFRPLAYGEGDGFYFASENSGLRMFTNKIRDVFPGEVFVVEEGEIYSKVLKKLPHSHCV
CCCCCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCEE
FEYIYFARPDSNIEGVSVYLARYRMGVELARESPAPGDVVIAVPDSGRTAALGFAHESGI
EEEEEEECCCCCCCHHHHEEHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHCCC
PYMEGLIKNRYIGRTFIMPSGRELKVKLKLSPVREVIEGKRVVLVDDSIVRGTTIKRIVK
HHHHHHHHCCCCCEEEECCCCCEEEEEEEECHHHHHHCCCEEEEECCCCCCCHHHHHHHH
MIRDAGAEEVHVRISSPPIRYPCYMGIDIPTRHELIAAWRTVEDIRREIGADSLAYLSIE
HHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
GLKRAVGIEDLCMACLTGHYPEWAFDFEL
HHHHHCCHHHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]