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The map label for this gene is moaA

Identifier: 18976462

GI number: 18976462

Start: 96627

End: 97550

Strand: Direct

Name: moaA

Synonym: PF0090

Alternate gene names: 18976462

Gene position: 96627-97550 (Clockwise)

Preceding gene: 18976461

Following gene: 18976463

Centisome position: 5.06

GC content: 39.72

Gene sequence:

>924_bases
ATGCTAATTGATAAGTTTGGCAGGCCAGTAACGAACCTTAGAATCTCCTTAACAAAGGAATGTAATTTGAACTGCTTCTA
TTGCCATAGGGAAGGCCAACAAGACGGAGAAAGAACAATGACCCCAGAAGAAATTGAAAGAATCGTGAGGATAGCTTCTA
GACTTGGGATTAGAAATGTTAAGCTAACGGGAGGAGAGCCGACTGTTAGACCTGACATCTATGAAATTATACAAAGGATA
AGGCCTTATGTTGTTGACCTTTCCATGACAACTAATGGGACAACACTCTATGCAAGCGCTGAAAAATTAAAAGAGGCTGG
CCTTGATAGGGTTAACATTAGCCTCGATACATTGGACAGGAAAAAGTATAAAATGATAACTGGCTACGATGTTTTAGATC
AGGTTTTGAAGGGAATTAGGAGGGCCACAAACCTTTTCTATCCAGTAAAGCTAAATATGGTAGTTATGAGGGGAATAAAC
GATGATGAAATTTGGGACATGATTAGATTTGCTGGGGAAGTTAATGCAATACTTCAGCTTATCGAAATAGAAGTTCCCAG
GGAAATGGAGAATTCTCAGTTCTTTAAGGATTTCTTTTACCCTTTAAAGCCATTGGAGGAAAAGTTCGAAAAGATTGCAG
TGGAAATTCGGGAGAGAAAGATGCATAGAAGGAGAAAATACTTTTTACCAGTTGATGGAAAGATGGTTGAAGTTGAAGTC
GTTCGATCAATGCACAACACAACCTTTTGCATGAACTGCACTAGGTTGAGACTAACGGCAGATGGTTACCTAAAGACCTG
CCTGCTGAGGAAAGATGACTTGATTGATATATTGGGCCCCATAAGGAGAGGAGCTACTGATGATGAACTTGTCAAGATAT
TTAAGAGAGCTGTGCAACTTAGGAAGCCCTATTGGATTAACTGA

Upstream 100 bases:

>100_bases
CATATATACAAGGTTTGTGCGGACATAGAGATTAAACAATAATGTTTACGTAAAAGTCTTTTATTTACTTTTTGCATTAA
AATATTGGAGGTTACAGTGG

Downstream 100 bases:

>100_bases
AGAAATGCTTTTAGGGAAGTTTAACAAAGTTATTATAAGGTGAAAAAATGGAAAAACTTGCAAATATTTTGGCGGGTTTG
ATTTTAATTATCCTTGGCAT

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLIDKFGRPVTNLRISLTKECNLNCFYCHREGQQDGERTMTPEEIERIVRIASRLGIRNVKLTGGEPTVRPDIYEIIQRI
RPYVVDLSMTTNGTTLYASAEKLKEAGLDRVNISLDTLDRKKYKMITGYDVLDQVLKGIRRATNLFYPVKLNMVVMRGIN
DDEIWDMIRFAGEVNAILQLIEIEVPREMENSQFFKDFFYPLKPLEEKFEKIAVEIRERKMHRRRKYFLPVDGKMVEVEV
VRSMHNTTFCMNCTRLRLTADGYLKTCLLRKDDLIDILGPIRRGATDDELVKIFKRAVQLRKPYWIN

Sequences:

>Translated_307_residues
MLIDKFGRPVTNLRISLTKECNLNCFYCHREGQQDGERTMTPEEIERIVRIASRLGIRNVKLTGGEPTVRPDIYEIIQRI
RPYVVDLSMTTNGTTLYASAEKLKEAGLDRVNISLDTLDRKKYKMITGYDVLDQVLKGIRRATNLFYPVKLNMVVMRGIN
DDEIWDMIRFAGEVNAILQLIEIEVPREMENSQFFKDFFYPLKPLEEKFEKIAVEIRERKMHRRRKYFLPVDGKMVEVEV
VRSMHNTTFCMNCTRLRLTADGYLKTCLLRKDDLIDILGPIRRGATDDELVKIFKRAVQLRKPYWIN
>Mature_307_residues
MLIDKFGRPVTNLRISLTKECNLNCFYCHREGQQDGERTMTPEEIERIVRIASRLGIRNVKLTGGEPTVRPDIYEIIQRI
RPYVVDLSMTTNGTTLYASAEKLKEAGLDRVNISLDTLDRKKYKMITGYDVLDQVLKGIRRATNLFYPVKLNMVVMRGIN
DDEIWDMIRFAGEVNAILQLIEIEVPREMENSQFFKDFFYPLKPLEEKFEKIAVEIRERKMHRRRKYFLPVDGKMVEVEV
VRSMHNTTFCMNCTRLRLTADGYLKTCLLRKDDLIDILGPIRRGATDDELVKIFKRAVQLRKPYWIN

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI22027536, Length=310, Percent_Identity=34.5161290322581, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI116734668, Length=310, Percent_Identity=34.5161290322581, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1786999, Length=277, Percent_Identity=31.4079422382672, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=33.6569579288026, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32566329, Length=309, Percent_Identity=33.6569579288026, Blast_Score=160, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17561210, Length=309, Percent_Identity=34.6278317152104, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=36.8589743589744, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI28574488, Length=312, Percent_Identity=36.8589743589744, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_PYRFU (Q8U4J5)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_577819.1
- ProteinModelPortal:   Q8U4J5
- SMR:   Q8U4J5
- EnsemblBacteria:   EBPYRT00000005432
- GeneID:   1467919
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0090
- NMPDR:   fig|186497.1.peg.90
- GeneTree:   EBGT00050000022159
- HOGENOM:   HBG529885
- OMA:   EMENSQF
- ProtClustDB:   PRK00164
- HAMAP:   MF_01225_A
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013485
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02668

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36022; Mature: 36022

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 14-14 BINDING 27-27 BINDING 61-61 BINDING 65-65 BINDING 90-90 BINDING 114-114 BINDING 150-150 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDKFGRPVTNLRISLTKECNLNCFYCHREGQQDGERTMTPEEIERIVRIASRLGIRNV
CCCCCCCCCHHCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
KLTGGEPTVRPDIYEIIQRIRPYVVDLSMTTNGTTLYASAEKLKEAGLDRVNISLDTLDR
EEECCCCCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECHHHHHHCCCCEEEEEHHHCCC
KKYKMITGYDVLDQVLKGIRRATNLFYPVKLNMVVMRGINDDEIWDMIRFAGEVNAILQL
HHHEEECCHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
IEIEVPREMENSQFFKDFFYPLKPLEEKFEKIAVEIRERKMHRRRKYFLPVDGKMVEVEV
HHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCEEHHHH
VRSMHNTTFCMNCTRLRLTADGYLKTCLLRKDDLIDILGPIRRGATDDELVKIFKRAVQL
HHHHHCCEEEEECEEEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RKPYWIN
CCCCCCC
>Mature Secondary Structure
MLIDKFGRPVTNLRISLTKECNLNCFYCHREGQQDGERTMTPEEIERIVRIASRLGIRNV
CCCCCCCCCHHCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
KLTGGEPTVRPDIYEIIQRIRPYVVDLSMTTNGTTLYASAEKLKEAGLDRVNISLDTLDR
EEECCCCCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECHHHHHHCCCCEEEEEHHHCCC
KKYKMITGYDVLDQVLKGIRRATNLFYPVKLNMVVMRGINDDEIWDMIRFAGEVNAILQL
HHHEEECCHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
IEIEVPREMENSQFFKDFFYPLKPLEEKFEKIAVEIRERKMHRRRKYFLPVDGKMVEVEV
HHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCEEHHHH
VRSMHNTTFCMNCTRLRLTADGYLKTCLLRKDDLIDILGPIRRGATDDELVKIFKRAVQL
HHHHHCCEEEEECEEEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RKPYWIN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA