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The map label for this gene is radB [H]

Identifier: 18976393

GI number: 18976393

Start: 23795

End: 24502

Strand: Direct

Name: radB [H]

Synonym: PF0021

Alternate gene names: 18976393

Gene position: 23795-24502 (Clockwise)

Preceding gene: 18976392

Following gene: 18976397

Centisome position: 1.25

GC content: 42.37

Gene sequence:

>708_bases
ATGAGGGTTTTGAAATCTGAAGGTGTTTCAATGCTAAATACTGAGCTCTTAACCACTGGAGTCAAGGGGTTAGATGAGCT
TTTAGGTGGTGGAGTTGCTAAGGGAGTAATACTCCAAGTTTACGGGCCATTTGCCACCGGGAAGACAACTTTTGCAATGC
AGGTTGGATTATTGAATGAGGGAAAAGTGGCTTATGTTGATACTGAGGGGGGATTCTCCCCCGAAAGGTTAGCTCAAATG
GCAGAATCAAGGAACTTGGATGTGGAGAAAGCACTTGAAAAGTTCGTGATATTCGAACCTATGGATTTAAACGAGCAAAG
ACAGGTAATTGCGAGGTTGAAAAATATCGTGAATGAAAAGTTTTCTTTAGTTGTGGTCGACTCCTTTACGGCCCATTATA
GAGCGGAGGGGAGTAGAGAGTATGGAGAACTTTCCAAGCAACTCCAAGTTCTTCAGTGGATTGCCAGAAGAAAAAACGTT
GCCGTTATAGTTGTCAATCAAGTTTATTACGATTCAAACTCAGGAATTCTTAAACCAATAGCTGAGCACACCCTGGGGTA
CAAAACAAAGGACATCCTCCGCTTTGAAAGGCTTAGGGTTGGAGTGAGAATTGCAGTTCTGGAAAGGCATAGGTTTAGGC
CAGAGGGTGGGATGGTATACTTCAAAATAACAGATAAAGGATTGGAGGATGTAAAAAACGAAGATTAG

Upstream 100 bases:

>100_bases
CCAATGCACTATGGAACGTACAGCGAGGCCGATCCTGAAGAGTTCAAGAAGGAGCTCCAAAAAAGGCGCATATGGGTTTT
AGTAAAGGATCTTAAGCCCT

Downstream 100 bases:

>100_bases
AGCCTGTCGTAGACCTCCTGGGCAATCCTCAGCGTTGCCTTATAGAGCTTCTCACTAATAATTCCCTCCTCGTAGTGTTC
CTTTAGCTTCTCCTTATCTA

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRVLKSEGVSMLNTELLTTGVKGLDELLGGGVAKGVILQVYGPFATGKTTFAMQVGLLNEGKVAYVDTEGGFSPERLAQM
AESRNLDVEKALEKFVIFEPMDLNEQRQVIARLKNIVNEKFSLVVVDSFTAHYRAEGSREYGELSKQLQVLQWIARRKNV
AVIVVNQVYYDSNSGILKPIAEHTLGYKTKDILRFERLRVGVRIAVLERHRFRPEGGMVYFKITDKGLEDVKNED

Sequences:

>Translated_235_residues
MRVLKSEGVSMLNTELLTTGVKGLDELLGGGVAKGVILQVYGPFATGKTTFAMQVGLLNEGKVAYVDTEGGFSPERLAQM
AESRNLDVEKALEKFVIFEPMDLNEQRQVIARLKNIVNEKFSLVVVDSFTAHYRAEGSREYGELSKQLQVLQWIARRKNV
AVIVVNQVYYDSNSGILKPIAEHTLGYKTKDILRFERLRVGVRIAVLERHRFRPEGGMVYFKITDKGLEDVKNED
>Mature_235_residues
MRVLKSEGVSMLNTELLTTGVKGLDELLGGGVAKGVILQVYGPFATGKTTFAMQVGLLNEGKVAYVDTEGGFSPERLAQM
AESRNLDVEKALEKFVIFEPMDLNEQRQVIARLKNIVNEKFSLVVVDSFTAHYRAEGSREYGELSKQLQVLQWIARRKNV
AVIVVNQVYYDSNSGILKPIAEHTLGYKTKDILRFERLRVGVRIAVLERHRFRPEGGMVYFKITDKGLEDVKNED

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19924133, Length=240, Percent_Identity=32.0833333333333, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI23238219, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI256017143, Length=227, Percent_Identity=30.3964757709251, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI256017141, Length=227, Percent_Identity=30.3964757709251, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI256017145, Length=149, Percent_Identity=36.9127516778523, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI10835029, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI19924117, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI46255039, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI217416415, Length=234, Percent_Identity=26.0683760683761, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71997301, Length=241, Percent_Identity=31.1203319502075, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71997295, Length=241, Percent_Identity=31.1203319502075, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321027, Length=240, Percent_Identity=32.9166666666667, Blast_Score=111, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320942, Length=243, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320207, Length=198, Percent_Identity=31.3131313131313, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864108, Length=241, Percent_Identity=31.5352697095436, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24651285, Length=241, Percent_Identity=30.2904564315353, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588
- InterPro:   IPR004504 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 26427; Mature: 26427

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLKSEGVSMLNTELLTTGVKGLDELLGGGVAKGVILQVYGPFATGKTTFAMQVGLLNE
CCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCCCEEEEEEEEECCC
GKVAYVDTEGGFSPERLAQMAESRNLDVEKALEKFVIFEPMDLNEQRQVIARLKNIVNEK
CCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCC
FSLVVVDSFTAHYRAEGSREYGELSKQLQVLQWIARRKNVAVIVVNQVYYDSNSGILKPI
EEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCHHHH
AEHTLGYKTKDILRFERLRVGVRIAVLERHRFRPEGGMVYFKITDKGLEDVKNED
HHHHCCCCHHHHHHHHHHHHHHEEEEEHHHCCCCCCCEEEEEECCCCHHHCCCCC
>Mature Secondary Structure
MRVLKSEGVSMLNTELLTTGVKGLDELLGGGVAKGVILQVYGPFATGKTTFAMQVGLLNE
CCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCCCEEEEEEEEECCC
GKVAYVDTEGGFSPERLAQMAESRNLDVEKALEKFVIFEPMDLNEQRQVIARLKNIVNEK
CCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCC
FSLVVVDSFTAHYRAEGSREYGELSKQLQVLQWIARRKNVAVIVVNQVYYDSNSGILKPI
EEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCHHHH
AEHTLGYKTKDILRFERLRVGVRIAVLERHRFRPEGGMVYFKITDKGLEDVKNED
HHHHCCCCHHHHHHHHHHHHHHEEEEEHHHCCCCCCCEEEEEECCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9348040; 10486005; 10903318 [H]