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The map label for this gene is asnB [H]

Identifier: 189424561

GI number: 189424561

Start: 1569585

End: 1571429

Strand: Reverse

Name: asnB [H]

Synonym: Glov_1501

Alternate gene names: 189424561

Gene position: 1571429-1569585 (Counterclockwise)

Preceding gene: 189424562

Following gene: 189424560

Centisome position: 40.11

GC content: 37.72

Gene sequence:

>1845_bases
ATGTGTGGAATCGCTGGGATTGTTTACAGAAATAAAAAAAGCAACCATTCAGAGCAGATAGCTGAAATGCTTAAGCTAAT
TCATCATCGCGGACCGGACGGGATGGGAGTTTTCGAAGCAGAGAATGTCGTGTTGGGGCATAGAAGGCTTGCTATCATTG
ATCTTAGCCCTAGCGGGCACCAGCCCATGTGCTACTTGGACCGTTTTATTATTACATTTAACGGTGAAATTTATAATTAT
CTAGAATTAAAGGAAGAATTATCCAATAAAGGGTATTACTTTATCTCTACATCTGACACAGAAGTCATTCTTGCAGCTTA
TGCCGAATGGGGTGAGGATTGTGTAACACACTTGAATGGAATGTTTGCTTTTGGTTTATATGATCTCTTGGAGCGCACCC
TTTTCCTTGCACGGGATAGGGTCGGAGAAAAGCCACTTTATTATTCTAAAAACGAAAACGAGTTCTTCTTTTTTTCTGAA
CCTAAACAGGTTATCTCTAGTGGAATTATCGAGGGCATTCCTAACGAAGTAGCAATTCGACAATATCTCGAATATCAGTT
TACGTTAAGCGCTCAAACTTTTTTTACAGGAATCTATAAACTCCTACCCGGACATTGCGCTACATTACGTAATGGTGTTT
TTAAAAGCCGGGAATACTGGAGCCTTGCAGACGTCGAGGTAGACTATTCCATATCCTATGCTGAGGCCAAGCTCAGGATA
AAAAACTTAGTAGAGGATTCATTGAAAATCCGTTTACGGTCTGATGTCCCAGTTGCTTCATACCTCAGTGGGGGTATCGA
TTCAACAATTATAGCTACATCGGCCGCAAAAGAATTATCCAGCCTTTCAACCTATACTTTTACATCAAAGAAATATCCGC
GATTTGATGAATCCCAAAATGCTCGCTTAACGGCAGATTTGATTCACGCTGATCATCATGAAATCGAAATAGAGAATGCG
GATACTCTTTCTTTGTGGCAGAATAGTACCTATTTCATGGATGAACCTGAAGTGGGCTATAGCTTGTTGTCGCAAATGGC
AGTCAGTCGTGAAGTGGCAAAGCAGACAAAGGTAGTGTTGGGTGGACAGGGCGGAGATGAGTTGTTTTTTGGTTATGCTT
GGTATAGTAATTTACTTTTGAAATCATTTTTATCATTCTCATCTGTAGCAGATTTCAAGTTATTAGATAAAGTCAAAGTA
GTCATAAGTTTTCTATTGAATTCACCTAAAAGAACTGCATTACGTTTAATCTTTGATTCTTTCAAGTCATTTGGCAGGCC
CCTTAGTGAAATTTATATTGATACGTGGAGAGGCTATGGTTGCTTCAGTTTGTTACAGGATATTAAAATAAAAGAAGGTG
CTAATTCTCCTGTAAGCGGATGCTTAAAATTGTCTGAATTAAAGAAATTTGAGTTTAATTATTGGCTTCACGGCTTACTA
CACGTCGAAGACAGATCTAGTATGGCTCATAGCCTTGAATCAAGAGTTCCATTGTTGGATCACCGTCTTGTAGAATTCGT
ATTCTCTCTCCCGCCCCATTTTATGATCGATGGTGTGCTAAACAAGAAGATATTTATTGATGCTTTTTCTGATATACTTC
CCAATCACGTTAAAAACAACAAACAGAAAATGGGCTATGTATCGCCGATTCATTCATGGCTTTCAGATCAGCATGTTACA
GCATTTATTAAAGATGTTATCAGCAATAAAAGCTCGTTTATTTACCACTTTGTTGATTATTATAAAATGAAATCATTGCA
GATAAATGACCGTCAATTATGGATGCTAATTTCATTAGAAATATGGCACAATATTTTTATAAAAAGAGATATTAAATCAT
GTTAA

Upstream 100 bases:

>100_bases
GACAATTGTAGGTGCTAACAGTGTTGTTAATAAATCTTTTCCACAAGGTAATGTTATTATTGCCGGTAGCCCTGCCAGAA
TAATTAAGGAACTATAACAA

Downstream 100 bases:

>100_bases
GCAAGTTAAAGCAACTACTGTTATTGTTTAGATATTTACGACTCAAATTAGATAAGCGTATCCATCTAAACGGATTTGTT
TCAATGTGCCCAAATGTGCG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MCGIAGIVYRNKKSNHSEQIAEMLKLIHHRGPDGMGVFEAENVVLGHRRLAIIDLSPSGHQPMCYLDRFIITFNGEIYNY
LELKEELSNKGYYFISTSDTEVILAAYAEWGEDCVTHLNGMFAFGLYDLLERTLFLARDRVGEKPLYYSKNENEFFFFSE
PKQVISSGIIEGIPNEVAIRQYLEYQFTLSAQTFFTGIYKLLPGHCATLRNGVFKSREYWSLADVEVDYSISYAEAKLRI
KNLVEDSLKIRLRSDVPVASYLSGGIDSTIIATSAAKELSSLSTYTFTSKKYPRFDESQNARLTADLIHADHHEIEIENA
DTLSLWQNSTYFMDEPEVGYSLLSQMAVSREVAKQTKVVLGGQGGDELFFGYAWYSNLLLKSFLSFSSVADFKLLDKVKV
VISFLLNSPKRTALRLIFDSFKSFGRPLSEIYIDTWRGYGCFSLLQDIKIKEGANSPVSGCLKLSELKKFEFNYWLHGLL
HVEDRSSMAHSLESRVPLLDHRLVEFVFSLPPHFMIDGVLNKKIFIDAFSDILPNHVKNNKQKMGYVSPIHSWLSDQHVT
AFIKDVISNKSSFIYHFVDYYKMKSLQINDRQLWMLISLEIWHNIFIKRDIKSC

Sequences:

>Translated_614_residues
MCGIAGIVYRNKKSNHSEQIAEMLKLIHHRGPDGMGVFEAENVVLGHRRLAIIDLSPSGHQPMCYLDRFIITFNGEIYNY
LELKEELSNKGYYFISTSDTEVILAAYAEWGEDCVTHLNGMFAFGLYDLLERTLFLARDRVGEKPLYYSKNENEFFFFSE
PKQVISSGIIEGIPNEVAIRQYLEYQFTLSAQTFFTGIYKLLPGHCATLRNGVFKSREYWSLADVEVDYSISYAEAKLRI
KNLVEDSLKIRLRSDVPVASYLSGGIDSTIIATSAAKELSSLSTYTFTSKKYPRFDESQNARLTADLIHADHHEIEIENA
DTLSLWQNSTYFMDEPEVGYSLLSQMAVSREVAKQTKVVLGGQGGDELFFGYAWYSNLLLKSFLSFSSVADFKLLDKVKV
VISFLLNSPKRTALRLIFDSFKSFGRPLSEIYIDTWRGYGCFSLLQDIKIKEGANSPVSGCLKLSELKKFEFNYWLHGLL
HVEDRSSMAHSLESRVPLLDHRLVEFVFSLPPHFMIDGVLNKKIFIDAFSDILPNHVKNNKQKMGYVSPIHSWLSDQHVT
AFIKDVISNKSSFIYHFVDYYKMKSLQINDRQLWMLISLEIWHNIFIKRDIKSC
>Mature_614_residues
MCGIAGIVYRNKKSNHSEQIAEMLKLIHHRGPDGMGVFEAENVVLGHRRLAIIDLSPSGHQPMCYLDRFIITFNGEIYNY
LELKEELSNKGYYFISTSDTEVILAAYAEWGEDCVTHLNGMFAFGLYDLLERTLFLARDRVGEKPLYYSKNENEFFFFSE
PKQVISSGIIEGIPNEVAIRQYLEYQFTLSAQTFFTGIYKLLPGHCATLRNGVFKSREYWSLADVEVDYSISYAEAKLRI
KNLVEDSLKIRLRSDVPVASYLSGGIDSTIIATSAAKELSSLSTYTFTSKKYPRFDESQNARLTADLIHADHHEIEIENA
DTLSLWQNSTYFMDEPEVGYSLLSQMAVSREVAKQTKVVLGGQGGDELFFGYAWYSNLLLKSFLSFSSVADFKLLDKVKV
VISFLLNSPKRTALRLIFDSFKSFGRPLSEIYIDTWRGYGCFSLLQDIKIKEGANSPVSGCLKLSELKKFEFNYWLHGLL
HVEDRSSMAHSLESRVPLLDHRLVEFVFSLPPHFMIDGVLNKKIFIDAFSDILPNHVKNNKQKMGYVSPIHSWLSDQHVT
AFIKDVISNKSSFIYHFVDYYKMKSLQINDRQLWMLISLEIWHNIFIKRDIKSC

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=412, Percent_Identity=26.2135922330097, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI168229252, Length=412, Percent_Identity=26.2135922330097, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI168229250, Length=412, Percent_Identity=26.2135922330097, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI296010848, Length=389, Percent_Identity=26.4781491002571, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786889, Length=392, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71993933, Length=397, Percent_Identity=28.9672544080605, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25147557, Length=397, Percent_Identity=28.9672544080605, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25147560, Length=357, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325403, Length=419, Percent_Identity=26.2529832935561, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=418, Percent_Identity=28.4688995215311, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45553209, Length=400, Percent_Identity=31, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70416; Mature: 70416

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIVYRNKKSNHSEQIAEMLKLIHHRGPDGMGVFEAENVVLGHRRLAIIDLSPSGH
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCEEEECCEEEEEEECCCCC
QPMCYLDRFIITFNGEIYNYLELKEELSNKGYYFISTSDTEVILAAYAEWGEDCVTHLNG
CCHHEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHH
MFAFGLYDLLERTLFLARDRVGEKPLYYSKNENEFFFFSEPKQVISSGIIEGIPNEVAIR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCHHHHHHHHHHHCCCHHHHHH
QYLEYQFTLSAQTFFTGIYKLLPGHCATLRNGVFKSREYWSLADVEVDYSISYAEAKLRI
HHHHHEEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECEEEEEEEEHHHHHHHH
KNLVEDSLKIRLRSDVPVASYLSGGIDSTIIATSAAKELSSLSTYTFTSKKYPRFDESQN
HHHHHHHHEEEECCCCCHHHHHCCCCCCHHEEHHHHHHHHHHHHEEECCCCCCCCCCCCC
ARLTADLIHADHHEIEIENADTLSLWQNSTYFMDEPEVGYSLLSQMAVSREVAKQTKVVL
CEEEEHHHCCCCCEEEECCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHCEEEE
GGQGGDELFFGYAWYSNLLLKSFLSFSSVADFKLLDKVKVVISFLLNSPKRTALRLIFDS
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FKSFGRPLSEIYIDTWRGYGCFSLLQDIKIKEGANSPVSGCLKLSELKKFEFNYWLHGLL
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEE
HVEDRSSMAHSLESRVPLLDHRLVEFVFSLPPHFMIDGVLNKKIFIDAFSDILPNHVKNN
EECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEHHHHHCCHHHCCC
KQKMGYVSPIHSWLSDQHVTAFIKDVISNKSSFIYHFVDYYKMKSLQINDRQLWMLISLE
HHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEECCCEEEEEEEHH
IWHNIFIKRDIKSC
HHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGIVYRNKKSNHSEQIAEMLKLIHHRGPDGMGVFEAENVVLGHRRLAIIDLSPSGH
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCEEEECCEEEEEEECCCCC
QPMCYLDRFIITFNGEIYNYLELKEELSNKGYYFISTSDTEVILAAYAEWGEDCVTHLNG
CCHHEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHH
MFAFGLYDLLERTLFLARDRVGEKPLYYSKNENEFFFFSEPKQVISSGIIEGIPNEVAIR
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCHHHHHHHHHHHCCCHHHHHH
QYLEYQFTLSAQTFFTGIYKLLPGHCATLRNGVFKSREYWSLADVEVDYSISYAEAKLRI
HHHHHEEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEECEEEEEEEEHHHHHHHH
KNLVEDSLKIRLRSDVPVASYLSGGIDSTIIATSAAKELSSLSTYTFTSKKYPRFDESQN
HHHHHHHHEEEECCCCCHHHHHCCCCCCHHEEHHHHHHHHHHHHEEECCCCCCCCCCCCC
ARLTADLIHADHHEIEIENADTLSLWQNSTYFMDEPEVGYSLLSQMAVSREVAKQTKVVL
CEEEEHHHCCCCCEEEECCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHCEEEE
GGQGGDELFFGYAWYSNLLLKSFLSFSSVADFKLLDKVKVVISFLLNSPKRTALRLIFDS
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FKSFGRPLSEIYIDTWRGYGCFSLLQDIKIKEGANSPVSGCLKLSELKKFEFNYWLHGLL
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEE
HVEDRSSMAHSLESRVPLLDHRLVEFVFSLPPHFMIDGVLNKKIFIDAFSDILPNHVKNN
EECCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEHHHHHCCHHHCCC
KQKMGYVSPIHSWLSDQHVTAFIKDVISNKSSFIYHFVDYYKMKSLQINDRQLWMLISLE
HHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEECCCEEEEEEEHH
IWHNIFIKRDIKSC
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]