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The map label for this gene is hyfR [H]

Identifier: 189424503

GI number: 189424503

Start: 1486047

End: 1487591

Strand: Reverse

Name: hyfR [H]

Synonym: Glov_1441

Alternate gene names: 189424503

Gene position: 1487591-1486047 (Counterclockwise)

Preceding gene: 189424516

Following gene: 189424499

Centisome position: 37.97

GC content: 51.07

Gene sequence:

>1545_bases
ATGAAAGTTAAACATGAAGAATTCTTTCGCGAAGTAACGCTAAGGTTTTGCAGCAGCCTGGATATCAATATCGCGATTCA
ACGCTGCTTCTTATACCTACGAGGAGTTCTGCCGGTCGATGAGATCTTTCTCGATATCCTCGACTTGCAATTGGGAGCCA
TACGACGCATTGCTGATGTGACCGTCGATGTTTCCCCCAAGCCGGCAGAGATCATACCGCTTCCTGAGGAGGTCTGGTTT
TGGGCTCAAAGTGTCCACGAACCGGTAATCGTAGACTCCACAAACAAAGATCCCATAGTGAGAAAAATTGCCGCACTCAC
CAAAAACGAAGGGAAATCAGATTTATCACTGCCATTGCACATCGAGGGCAAAAAGGTTGGGGTTCTCATTCTAAGGGCTA
ATGGTGACAGAGTTTACCGTAAGGAACATGCGGACCTGATTCGCCTGGTTGCTGAACCGATAGCCATCGCCTTATCAAAC
GCCCTTGCTCACGAGGAGTTGCTGCACTTGCGAGATGTACTTCTTGATGACAATCGTTTCCTGCAAAGGGAACTCTCTCC
CGGTTCTGATGGCGGCGACATCGTGGGAGAGCAGAGCGGCCTGCGTAACGTGATGGAACAGGTCCGGCAAGTGGCTCCAC
TCAACAATACCGTTCTGCTGCTTGGAGAGACCGGCGTCGGCAAAGAAGTCATTGCAAACGCCATCCACTATGGATCGACG
CGTAGTAGCGGTCCCTTTGTCAAGGTCAATTGCGGAGCCATCGCAGATACACTAATCGACAGCGAGCTTTTCGGGCACGA
AAAGGGGGCCTTTACCGGGGCAGTTGCGGAAAAACGAGGGCGTTTTGAGCGTGCTAACGGCGGGACACTTTTTTTGGACG
AGATTGGGGAACTCCCGCTCCAGGCTCAGGTAAAACTGTTGCGGGTGCTACAGAACCGCGAGCTTGAACGAGTGGGAGGG
GCCAAACCTATTCCGATCAACATCAGGATCATCGCTGCAACCAACAGGAATCTTGAACAAATGATCACGGAAGGTCATTT
TCGAGAAGATCTTTGGTTTCGAATAAATGTCTTCCCTATTTTTATCCCCCCACTAAGGCAGCGCCGTGAGGATATCCCGG
CACTGGCTAGGCACTTGGTGCAGGTAAAGAGCAGGGAACTTGGACTGACGGAGGTGCCAGCCATTGCATTGGGCGCTTTG
GAGCGCCTCATGATCTGCGACTGGCCTGGAAACGTGCGGGAACTGCAAAATATTGTGGAACGTGAACTAATCCGCCACAG
AGGAGGTTCTCTTAGCTTTGAATCGCTGTTGCCCCCCTTATCTCCCCAGAAAGAGCCACCTTCATCAAATGTGAATCAGG
AACTCGAAAAACCTGAAAGCCTCGACGAGGCCATGGCCCATCACATCAGAAAAGTCATTACATTCACAAAGGGAAAGATT
AACGGAACCGGTGGCGCGGCAGAGTTGCTTCGTATCAATCCAAGCACATTAAGGAGCCGGATGGCAAAGCTCGGGGTGAA
TCGAAAAGAAGGTGGAAACCCGTAA

Upstream 100 bases:

>100_bases
TGCGATTGAAAAGCAATGCTAATATCGATATTTAGATATTTTTGTTGTGACATTTCGATACCGTATGTATTTTATCGATA
TATCGAGAGGAGCGTGTCCC

Downstream 100 bases:

>100_bases
TGTACTCCACCAGCGCCGCCTCGAGCGGCGCTCATTTTTGTAAAGCAGTAGTTGAAGGGTAAAAACTTTCACACTAAGAC
GCCAGATCTGCCGCTTTAGT

Product: phytochrome sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKVKHEEFFREVTLRFCSSLDINIAIQRCFLYLRGVLPVDEIFLDILDLQLGAIRRIADVTVDVSPKPAEIIPLPEEVWF
WAQSVHEPVIVDSTNKDPIVRKIAALTKNEGKSDLSLPLHIEGKKVGVLILRANGDRVYRKEHADLIRLVAEPIAIALSN
ALAHEELLHLRDVLLDDNRFLQRELSPGSDGGDIVGEQSGLRNVMEQVRQVAPLNNTVLLLGETGVGKEVIANAIHYGST
RSSGPFVKVNCGAIADTLIDSELFGHEKGAFTGAVAEKRGRFERANGGTLFLDEIGELPLQAQVKLLRVLQNRELERVGG
AKPIPINIRIIAATNRNLEQMITEGHFREDLWFRINVFPIFIPPLRQRREDIPALARHLVQVKSRELGLTEVPAIALGAL
ERLMICDWPGNVRELQNIVERELIRHRGGSLSFESLLPPLSPQKEPPSSNVNQELEKPESLDEAMAHHIRKVITFTKGKI
NGTGGAAELLRINPSTLRSRMAKLGVNRKEGGNP

Sequences:

>Translated_514_residues
MKVKHEEFFREVTLRFCSSLDINIAIQRCFLYLRGVLPVDEIFLDILDLQLGAIRRIADVTVDVSPKPAEIIPLPEEVWF
WAQSVHEPVIVDSTNKDPIVRKIAALTKNEGKSDLSLPLHIEGKKVGVLILRANGDRVYRKEHADLIRLVAEPIAIALSN
ALAHEELLHLRDVLLDDNRFLQRELSPGSDGGDIVGEQSGLRNVMEQVRQVAPLNNTVLLLGETGVGKEVIANAIHYGST
RSSGPFVKVNCGAIADTLIDSELFGHEKGAFTGAVAEKRGRFERANGGTLFLDEIGELPLQAQVKLLRVLQNRELERVGG
AKPIPINIRIIAATNRNLEQMITEGHFREDLWFRINVFPIFIPPLRQRREDIPALARHLVQVKSRELGLTEVPAIALGAL
ERLMICDWPGNVRELQNIVERELIRHRGGSLSFESLLPPLSPQKEPPSSNVNQELEKPESLDEAMAHHIRKVITFTKGKI
NGTGGAAELLRINPSTLRSRMAKLGVNRKEGGNP
>Mature_514_residues
MKVKHEEFFREVTLRFCSSLDINIAIQRCFLYLRGVLPVDEIFLDILDLQLGAIRRIADVTVDVSPKPAEIIPLPEEVWF
WAQSVHEPVIVDSTNKDPIVRKIAALTKNEGKSDLSLPLHIEGKKVGVLILRANGDRVYRKEHADLIRLVAEPIAIALSN
ALAHEELLHLRDVLLDDNRFLQRELSPGSDGGDIVGEQSGLRNVMEQVRQVAPLNNTVLLLGETGVGKEVIANAIHYGST
RSSGPFVKVNCGAIADTLIDSELFGHEKGAFTGAVAEKRGRFERANGGTLFLDEIGELPLQAQVKLLRVLQNRELERVGG
AKPIPINIRIIAATNRNLEQMITEGHFREDLWFRINVFPIFIPPLRQRREDIPALARHLVQVKSRELGLTEVPAIALGAL
ERLMICDWPGNVRELQNIVERELIRHRGGSLSFESLLPPLSPQKEPPSSNVNQELEKPESLDEAMAHHIRKVITFTKGKI
NGTGGAAELLRINPSTLRSRMAKLGVNRKEGGNP

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=423, Percent_Identity=44.9172576832151, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1789087, Length=416, Percent_Identity=42.5480769230769, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788550, Length=261, Percent_Identity=50.1915708812261, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1790299, Length=270, Percent_Identity=48.8888888888889, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=41.3793103448276, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1790437, Length=263, Percent_Identity=45.2471482889734, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=42.7215189873418, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788905, Length=218, Percent_Identity=50.9174311926605, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=47.1311475409836, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=43.8297872340426, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=33.7539432176656, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=32.1070234113712, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=38.7665198237885, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 57196; Mature: 57196

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKHEEFFREVTLRFCSSLDINIAIQRCFLYLRGVLPVDEIFLDILDLQLGAIRRIADV
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHE
TVDVSPKPAEIIPLPEEVWFWAQSVHEPVIVDSTNKDPIVRKIAALTKNEGKSDLSLPLH
EEECCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEE
IEGKKVGVLILRANGDRVYRKEHADLIRLVAEPIAIALSNALAHEELLHLRDVLLDDNRF
ECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LQRELSPGSDGGDIVGEQSGLRNVMEQVRQVAPLNNTVLLLGETGVGKEVIANAIHYGST
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCC
RSSGPFVKVNCGAIADTLIDSELFGHEKGAFTGAVAEKRGRFERANGGTLFLDEIGELPL
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEHHHHCCCCH
QAQVKLLRVLQNRELERVGGAKPIPINIRIIAATNRNLEQMITEGHFREDLWFRINVFPI
HHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCEEEEEEEEEE
FIPPLRQRREDIPALARHLVQVKSRELGLTEVPAIALGALERLMICDWPGNVRELQNIVE
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHEEECCCCCHHHHHHHHH
RELIRHRGGSLSFESLLPPLSPQKEPPSSNVNQELEKPESLDEAMAHHIRKVITFTKGKI
HHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCC
NGTGGAAELLRINPSTLRSRMAKLGVNRKEGGNP
CCCCCCHHEEEECHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKVKHEEFFREVTLRFCSSLDINIAIQRCFLYLRGVLPVDEIFLDILDLQLGAIRRIADV
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHE
TVDVSPKPAEIIPLPEEVWFWAQSVHEPVIVDSTNKDPIVRKIAALTKNEGKSDLSLPLH
EEECCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEE
IEGKKVGVLILRANGDRVYRKEHADLIRLVAEPIAIALSNALAHEELLHLRDVLLDDNRF
ECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LQRELSPGSDGGDIVGEQSGLRNVMEQVRQVAPLNNTVLLLGETGVGKEVIANAIHYGST
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCC
RSSGPFVKVNCGAIADTLIDSELFGHEKGAFTGAVAEKRGRFERANGGTLFLDEIGELPL
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEHHHHCCCCH
QAQVKLLRVLQNRELERVGGAKPIPINIRIIAATNRNLEQMITEGHFREDLWFRINVFPI
HHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEECCCCHHHHHHCCCCCCCEEEEEEEEEE
FIPPLRQRREDIPALARHLVQVKSRELGLTEVPAIALGALERLMICDWPGNVRELQNIVE
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHEEECCCCCHHHHHHHHH
RELIRHRGGSLSFESLLPPLSPQKEPPSSNVNQELEKPESLDEAMAHHIRKVITFTKGKI
HHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHCCCC
NGTGGAAELLRINPSTLRSRMAKLGVNRKEGGNP
CCCCCCHHEEEECHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]