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The map label for this gene is pilJ [H]

Identifier: 189424351

GI number: 189424351

Start: 1334596

End: 1336212

Strand: Reverse

Name: pilJ [H]

Synonym: Glov_1286

Alternate gene names: 189424351

Gene position: 1336212-1334596 (Counterclockwise)

Preceding gene: 189424352

Following gene: 189424350

Centisome position: 34.11

GC content: 50.9

Gene sequence:

>1617_bases
ATGTCACAACATGAACGTACATCTTCACCTATACAAGCAGGTAGAAGAACTGGAATTGCTACAAAGATTCTCCTTGTGGC
AACTCTTGCTCTGATAACAGGTCTCGTCATCGTGGGCGGATCCGCCCTGTATCTGGAAAAACAGGCGCTGGTCGGTCTAC
AGCGAGACAACAGCCTGGCCTTTGTCAATATCATGGGCGATGATATCAAATCGGCGATGATGGCTGGCGACATGAAAAAA
GTCGATGACTATATCAAGGAGGTGATTGAACAGAAGCGAGCATTGGCTTTCTCCATTTATAACGACAAAGGTGAACTGCG
GGGTAACAAAACAAAGGGCGACGAACTGGTGACAAACGTCATGAGAAACAACAAACCCGCTAGTTTTGAAAAAACTTTAA
ACGGAACACATGTTCTTGACACCATATTGCCACTTGCCAATGAAGAACGCTGCCAGGCTTGTCATAGTAAAGAGACCATG
ATGTTAGGAGCTCTCAAACTTACCACTAGCATCGAGCAAGGCTATAGCTCCAGTAAAAAAGCCGCCCTCTGGCTCTTGGC
CTCCGGTGGGGTGGCGCTTGCTGTCAGCTTTATCTGTTTGCTGATTGTCCTGCGGGCAACCGTTACAAAGAGGCTGAACG
ACTTTGTTGCCAAGGTGACCGACCTTGCCCGGGGTGAGGGCGATCTCACCAAAATGATCGCCGTAACATCCAATGATGAA
TTTGGCCAACTGGCAGATGAAATCAACTCTCTTGTGACGAAAATCAGGAATATTATCAATCAGATTGCCCAGACAAGCGA
TCAGGTTTCCTCATCATCCGCCCAACTCCAATCCAATGCTTCCCAGATGGCCGCAGGTGCTGAAAATGTAGCTGCCCAAG
CGGAAACTGTTGCCACTGCAGGGGAAGAGATGACTGCCACATCAAGCGATATTGCCCAAAACTGCCTGCTGGCCTCGGAG
GGCTCGCAGCGGGCCAGTGCTGCAGCAGCTTCCGGTGCAGCGGTTGTGAGTGATACGATTTCAGTCATGCACAGCATTAC
CGAGCGGGTCAAAAACTCTGCAAAAGCGGTTGAAAGCCTGGGAAGCCGTTCCGATCAGATTGGCGAGATCGTCGGCACCA
TTGAGGACATTGCCGACCAGACCAATTTGCTGGCGCTGAATGCCGCCATCGAGGCTGCCCGGGCCGGTGAACAGGGCAGA
GGTTTTGCCGTTGTAGCCGATGAAGTGCGAGCCCTGGCAGAACGTACCACGCGAGCGACCCGGGAAATTGGCGAGATGAT
CAAAGCCATCCAGCATGAAACCAAGGATGCTGTGCTCGCCATGCACGAAGGGGTCAGTGAAGTTGCCAGAGGAAGCGAGA
AGGCATCCGACTCCGGCAAAGCTCTTGAAGAGATCCTCCAACAGATCAGCGACGTTAGTATGCAGATTCAGCAGGTTGCC
ACCGCAGCAGAAGAACAAACGGCTACAACTTCTGAAATCAGTAGCAATATGCAGCAGATTACCGAGGTTATCGCAAATAC
ATCAAAAGGTTCCAAGGATTCCGCTGCTGCCGCCAATCACCTTGCCATACTATCCGACGATCTGCGACGAATCGTGAGTC
AGTTCAAAATCGTATAA

Upstream 100 bases:

>100_bases
GTCCACGAATTAGTCGTTACACTCAATTCCAAGTTGACTGACATGGAAGCGCAATGGAGACCACAAGCAACAAACTTATT
TACCATCAGGGAGAACTAAC

Downstream 100 bases:

>100_bases
ACCGTATTTGGCATGGTGTCCGGTGCAGTGCTTTCACGTTGATAGAGTTTGTCATAATGCGTGAATATGGAATCGAAGGG
GGTGCTCTATACAAATTGTC

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSQHERTSSPIQAGRRTGIATKILLVATLALITGLVIVGGSALYLEKQALVGLQRDNSLAFVNIMGDDIKSAMMAGDMKK
VDDYIKEVIEQKRALAFSIYNDKGELRGNKTKGDELVTNVMRNNKPASFEKTLNGTHVLDTILPLANEERCQACHSKETM
MLGALKLTTSIEQGYSSSKKAALWLLASGGVALAVSFICLLIVLRATVTKRLNDFVAKVTDLARGEGDLTKMIAVTSNDE
FGQLADEINSLVTKIRNIINQIAQTSDQVSSSSAQLQSNASQMAAGAENVAAQAETVATAGEEMTATSSDIAQNCLLASE
GSQRASAAAASGAAVVSDTISVMHSITERVKNSAKAVESLGSRSDQIGEIVGTIEDIADQTNLLALNAAIEAARAGEQGR
GFAVVADEVRALAERTTRATREIGEMIKAIQHETKDAVLAMHEGVSEVARGSEKASDSGKALEEILQQISDVSMQIQQVA
TAAEEQTATTSEISSNMQQITEVIANTSKGSKDSAAAANHLAILSDDLRRIVSQFKIV

Sequences:

>Translated_538_residues
MSQHERTSSPIQAGRRTGIATKILLVATLALITGLVIVGGSALYLEKQALVGLQRDNSLAFVNIMGDDIKSAMMAGDMKK
VDDYIKEVIEQKRALAFSIYNDKGELRGNKTKGDELVTNVMRNNKPASFEKTLNGTHVLDTILPLANEERCQACHSKETM
MLGALKLTTSIEQGYSSSKKAALWLLASGGVALAVSFICLLIVLRATVTKRLNDFVAKVTDLARGEGDLTKMIAVTSNDE
FGQLADEINSLVTKIRNIINQIAQTSDQVSSSSAQLQSNASQMAAGAENVAAQAETVATAGEEMTATSSDIAQNCLLASE
GSQRASAAAASGAAVVSDTISVMHSITERVKNSAKAVESLGSRSDQIGEIVGTIEDIADQTNLLALNAAIEAARAGEQGR
GFAVVADEVRALAERTTRATREIGEMIKAIQHETKDAVLAMHEGVSEVARGSEKASDSGKALEEILQQISDVSMQIQQVA
TAAEEQTATTSEISSNMQQITEVIANTSKGSKDSAAAANHLAILSDDLRRIVSQFKIV
>Mature_537_residues
SQHERTSSPIQAGRRTGIATKILLVATLALITGLVIVGGSALYLEKQALVGLQRDNSLAFVNIMGDDIKSAMMAGDMKKV
DDYIKEVIEQKRALAFSIYNDKGELRGNKTKGDELVTNVMRNNKPASFEKTLNGTHVLDTILPLANEERCQACHSKETMM
LGALKLTTSIEQGYSSSKKAALWLLASGGVALAVSFICLLIVLRATVTKRLNDFVAKVTDLARGEGDLTKMIAVTSNDEF
GQLADEINSLVTKIRNIINQIAQTSDQVSSSSAQLQSNASQMAAGAENVAAQAETVATAGEEMTATSSDIAQNCLLASEG
SQRASAAAASGAAVVSDTISVMHSITERVKNSAKAVESLGSRSDQIGEIVGTIEDIADQTNLLALNAAIEAARAGEQGRG
FAVVADEVRALAERTTRATREIGEMIKAIQHETKDAVLAMHEGVSEVARGSEKASDSGKALEEILQQISDVSMQIQQVAT
AAEEQTATTSEISSNMQQITEVIANTSKGSKDSAAAANHLAILSDDLRRIVSQFKIV

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=312, Percent_Identity=30.1282051282051, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787690, Length=423, Percent_Identity=27.1867612293144, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788195, Length=302, Percent_Identity=29.8013245033113, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788194, Length=311, Percent_Identity=29.903536977492, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789453, Length=330, Percent_Identity=28.7878787878788, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 56998; Mature: 56867

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50885 HAMP ; PS00061 ADH_SHORT ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHERTSSPIQAGRRTGIATKILLVATLALITGLVIVGGSALYLEKQALVGLQRDNSLA
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHCEECCCCEE
FVNIMGDDIKSAMMAGDMKKVDDYIKEVIEQKRALAFSIYNDKGELRGNKTKGDELVTNV
EEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHH
MRNNKPASFEKTLNGTHVLDTILPLANEERCQACHSKETMMLGALKLTTSIEQGYSSSKK
HHCCCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AALWLLASGGVALAVSFICLLIVLRATVTKRLNDFVAKVTDLARGEGDLTKMIAVTSNDE
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCH
FGQLADEINSLVTKIRNIINQIAQTSDQVSSSSAQLQSNASQMAAGAENVAAQAETVATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GEEMTATSSDIAQNCLLASEGSQRASAAAASGAAVVSDTISVMHSITERVKNSAKAVESL
CCHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSRSDQIGEIVGTIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRALAERTTRAT
CCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
REIGEMIKAIQHETKDAVLAMHEGVSEVARGSEKASDSGKALEEILQQISDVSMQIQQVA
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAAEEQTATTSEISSNMQQITEVIANTSKGSKDSAAAANHLAILSDDLRRIVSQFKIV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQHERTSSPIQAGRRTGIATKILLVATLALITGLVIVGGSALYLEKQALVGLQRDNSLA
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHCEECCCCEE
FVNIMGDDIKSAMMAGDMKKVDDYIKEVIEQKRALAFSIYNDKGELRGNKTKGDELVTNV
EEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHH
MRNNKPASFEKTLNGTHVLDTILPLANEERCQACHSKETMMLGALKLTTSIEQGYSSSKK
HHCCCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AALWLLASGGVALAVSFICLLIVLRATVTKRLNDFVAKVTDLARGEGDLTKMIAVTSNDE
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCH
FGQLADEINSLVTKIRNIINQIAQTSDQVSSSSAQLQSNASQMAAGAENVAAQAETVATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GEEMTATSSDIAQNCLLASEGSQRASAAAASGAAVVSDTISVMHSITERVKNSAKAVESL
CCHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSRSDQIGEIVGTIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRALAERTTRAT
CCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
REIGEMIKAIQHETKDAVLAMHEGVSEVARGSEKASDSGKALEEILQQISDVSMQIQQVA
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAAEEQTATTSEISSNMQQITEVIANTSKGSKDSAAAANHLAILSDDLRRIVSQFKIV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]