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The map label for this gene is trpB2 [H]

Identifier: 189424278

GI number: 189424278

Start: 1259051

End: 1260406

Strand: Reverse

Name: trpB2 [H]

Synonym: Glov_1213

Alternate gene names: 189424278

Gene position: 1260406-1259051 (Counterclockwise)

Preceding gene: 189424279

Following gene: 189424277

Centisome position: 32.17

GC content: 60.18

Gene sequence:

>1356_bases
ATGCAGACAAAGATAATTTTGCCCGAGGATAAACTCCCCACCCAATGGTACAACATCATCCCTGACCTGCCAGGTCCGCT
GGCACCGGTTATCTCCCCACGTACCGGAGAGCCGGTCACCCCGGAAGAGCTGCTGGCGATCTTTCCGCCTGCCATTCTGG
AGCAGGAGATGTCCAATCAGCGCTGGATCGACATCCCTGAGCAGGTGCGTGACATCTATACCATGTGGCGGCCATCCCCG
ATGTTCCGGGCTCACCGCCTGGAAAAGGCGCTGGGCACGCCGGCCAAGATCTACTACAAGTACGAAGGGGTCTCACCGGC
CGGCTCACACAAGCCTAACTCGGCTATCCCCCAGGCCTTTTACAACAAGCAGGCCGGGATCAAGCGGCTGGCCACCGAGA
CCGGCGCCGGTCAATGGGGCAGTTCCCTGTCACTGGCCTGTTCCCTGTTCGGTCTGGAATGTACGGTCTACATGGTCAAG
GTTTCCTACTTCCAGAAGCCGTACCGCAAATCCATGATGCAGATGTGGGGCGCCACGGTGTACCCCTCCCCCTCTGAAAT
GACCGAGGCAGGCCGCGCTGCTCTGGCCGAAAACCCGGACTCCAACGGCAGCCTCGGACTGGCCATCTCTGAGGCGGTGG
AGGATGCCGCTACCCACAATGATACCAACTATGCCCTGGGCAGCGTGCTGAACCATGTCTGCATGCATCAGACCGTGATC
GGCCTGGAGGCGCTGGAGCAGTTCAAGATGGTGGGAGACTACCCGGATGTGGTGATCGCCTGCTGCGGCGGCGGCTCCAA
CTTCGCCGGTCTGGCCTTCCCGTTCCTGTCTGAAAAGGCCAATGGCAAAAAGGTACGCTGCCTGGCGGTGGAACCGGCCT
CCTGCCCTACCCTGACCAAAGGTGTCTATGACTTTGACTACGGCGACACCGCCAAGCTGGGACCGATTGCCAAGATGTAC
ACCCTGGGCCATGACTTCATGCCGCCCGGCATCCATGCCGGTGGTCTGCGCTACCACGGCGAGTCTCCGCTGGTTTCCCA
ACTCTATGCAGCCGGCCTGATCGAGGCCCGTTCCCTGATGCAGACCGCCTGCTTTGAAGGGGCCCTGCTGTTTGCCAAGA
ACGAGGGGATTGTACCGGCGCCGGAATCATCCCACGCCGTCAGGGCAGCCATTGATGAGGCGCTGCTGGCCAAGGAAGAG
GGCAAGGAACGGACCATCCTGTTCAACCTGTCCGGCCACGGCTTCATGGATATGACCGCCTATGACAACTACCTGGCCGG
CAACCTGGAAGACTACGAGTATCCGGCAGAGATGGTGCAGGAGTCATTAAAGCATCTGCCGAAGGTGAAACTCTAG

Upstream 100 bases:

>100_bases
CACGACATGGCGCGGAGGCGTACCGAACAGTACGCCGCACAAGACATGGCACAGCATGGCGTGGTCAGGACGGAAAAGAA
CCGTTCCCTTCGGAGGATCG

Downstream 100 bases:

>100_bases
GTTGAGATCGAGGTTAAGGTTGAGCAATTTCCTTAACCTCAACCTTAACCTTCATCTGGTGTTTCTATGATCAAAGTAAA
AATCTGTGGCATAACCAGCC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQTKIILPEDKLPTQWYNIIPDLPGPLAPVISPRTGEPVTPEELLAIFPPAILEQEMSNQRWIDIPEQVRDIYTMWRPSP
MFRAHRLEKALGTPAKIYYKYEGVSPAGSHKPNSAIPQAFYNKQAGIKRLATETGAGQWGSSLSLACSLFGLECTVYMVK
VSYFQKPYRKSMMQMWGATVYPSPSEMTEAGRAALAENPDSNGSLGLAISEAVEDAATHNDTNYALGSVLNHVCMHQTVI
GLEALEQFKMVGDYPDVVIACCGGGSNFAGLAFPFLSEKANGKKVRCLAVEPASCPTLTKGVYDFDYGDTAKLGPIAKMY
TLGHDFMPPGIHAGGLRYHGESPLVSQLYAAGLIEARSLMQTACFEGALLFAKNEGIVPAPESSHAVRAAIDEALLAKEE
GKERTILFNLSGHGFMDMTAYDNYLAGNLEDYEYPAEMVQESLKHLPKVKL

Sequences:

>Translated_451_residues
MQTKIILPEDKLPTQWYNIIPDLPGPLAPVISPRTGEPVTPEELLAIFPPAILEQEMSNQRWIDIPEQVRDIYTMWRPSP
MFRAHRLEKALGTPAKIYYKYEGVSPAGSHKPNSAIPQAFYNKQAGIKRLATETGAGQWGSSLSLACSLFGLECTVYMVK
VSYFQKPYRKSMMQMWGATVYPSPSEMTEAGRAALAENPDSNGSLGLAISEAVEDAATHNDTNYALGSVLNHVCMHQTVI
GLEALEQFKMVGDYPDVVIACCGGGSNFAGLAFPFLSEKANGKKVRCLAVEPASCPTLTKGVYDFDYGDTAKLGPIAKMY
TLGHDFMPPGIHAGGLRYHGESPLVSQLYAAGLIEARSLMQTACFEGALLFAKNEGIVPAPESSHAVRAAIDEALLAKEE
GKERTILFNLSGHGFMDMTAYDNYLAGNLEDYEYPAEMVQESLKHLPKVKL
>Mature_451_residues
MQTKIILPEDKLPTQWYNIIPDLPGPLAPVISPRTGEPVTPEELLAIFPPAILEQEMSNQRWIDIPEQVRDIYTMWRPSP
MFRAHRLEKALGTPAKIYYKYEGVSPAGSHKPNSAIPQAFYNKQAGIKRLATETGAGQWGSSLSLACSLFGLECTVYMVK
VSYFQKPYRKSMMQMWGATVYPSPSEMTEAGRAALAENPDSNGSLGLAISEAVEDAATHNDTNYALGSVLNHVCMHQTVI
GLEALEQFKMVGDYPDVVIACCGGGSNFAGLAFPFLSEKANGKKVRCLAVEPASCPTLTKGVYDFDYGDTAKLGPIAKMY
TLGHDFMPPGIHAGGLRYHGESPLVSQLYAAGLIEARSLMQTACFEGALLFAKNEGIVPAPESSHAVRAAIDEALLAKEE
GKERTILFNLSGHGFMDMTAYDNYLAGNLEDYEYPAEMVQESLKHLPKVKL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=399, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=378, Percent_Identity=32.010582010582, Blast_Score=129, Evalue=9e-31,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49283; Mature: 49283

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKIILPEDKLPTQWYNIIPDLPGPLAPVISPRTGEPVTPEELLAIFPPAILEQEMSNQ
CCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCC
RWIDIPEQVRDIYTMWRPSPMFRAHRLEKALGTPAKIYYKYEGVSPAGSHKPNSAIPQAF
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHH
YNKQAGIKRLATETGAGQWGSSLSLACSLFGLECTVYMVKVSYFQKPYRKSMMQMWGATV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEEEHHHHHHCHHHHHHHHHHCCCC
YPSPSEMTEAGRAALAENPDSNGSLGLAISEAVEDAATHNDTNYALGSVLNHVCMHQTVI
CCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GLEALEQFKMVGDYPDVVIACCGGGSNFAGLAFPFLSEKANGKKVRCLAVEPASCPTLTK
HHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHC
GVYDFDYGDTAKLGPIAKMYTLGHDFMPPGIHAGGLRYHGESPLVSQLYAAGLIEARSLM
CCEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
QTACFEGALLFAKNEGIVPAPESSHAVRAAIDEALLAKEEGKERTILFNLSGHGFMDMTA
HHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHH
YDNYLAGNLEDYEYPAEMVQESLKHLPKVKL
CCCHHCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTKIILPEDKLPTQWYNIIPDLPGPLAPVISPRTGEPVTPEELLAIFPPAILEQEMSNQ
CCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCCC
RWIDIPEQVRDIYTMWRPSPMFRAHRLEKALGTPAKIYYKYEGVSPAGSHKPNSAIPQAF
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHH
YNKQAGIKRLATETGAGQWGSSLSLACSLFGLECTVYMVKVSYFQKPYRKSMMQMWGATV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEEEHHHHHHCHHHHHHHHHHCCCC
YPSPSEMTEAGRAALAENPDSNGSLGLAISEAVEDAATHNDTNYALGSVLNHVCMHQTVI
CCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GLEALEQFKMVGDYPDVVIACCGGGSNFAGLAFPFLSEKANGKKVRCLAVEPASCPTLTK
HHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHC
GVYDFDYGDTAKLGPIAKMYTLGHDFMPPGIHAGGLRYHGESPLVSQLYAAGLIEARSLM
CCEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
QTACFEGALLFAKNEGIVPAPESSHAVRAAIDEALLAKEEGKERTILFNLSGHGFMDMTA
HHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEHH
YDNYLAGNLEDYEYPAEMVQESLKHLPKVKL
CCCHHCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]