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The map label for this gene is psrB [H]

Identifier: 189424228

GI number: 189424228

Start: 1216622

End: 1217179

Strand: Reverse

Name: psrB [H]

Synonym: Glov_1163

Alternate gene names: 189424228

Gene position: 1217179-1216622 (Counterclockwise)

Preceding gene: 189424229

Following gene: 189424227

Centisome position: 31.07

GC content: 59.14

Gene sequence:

>558_bases
ATGCCGAAGTATGCAATGGTAATCGATCAACGGCGCTGCATCGGCTGCATGGCCTGCATTGTCGCCTGCAAGGCGGAGAA
CAACGTACCGCCCGAGCAGTTTCGCACACGGGTGCTGGAGAAAACTGAAGGACAGTTTCCCAATCTGCGCACTGAATTGC
GCTCCGAGCTGTGCAACCACTGTGACAACCCGCCCTGTGTCTACAACTGCCCTACCCGCGCCTCCTACAAAAGCCGCCTG
AGCGGCCTGGTACTGCTGGACAAGAAACGCTGTGTGGGTTGCAAGGCCTGTGTGGCTGCCTGCCCCTATGACGCCCGCTA
CATTGACCACAACAAGGGGTTTGCCGACAAATGCACCTTCTGCGACCACCGGATCAAGGAAGGCAAGGAGCCGGCCTGTG
TAGCCACCTGTATCGGCAAGTCCCGCATCTTTGGTGATCTGAATGACCCGAAAAGCCCGGTGCGGCTGGCCCTGAAGGGG
CACCATGCCGAACTGCCGATGAAAAGCGCCGGCACCGAGCCACGGGTCTATTACATCCGCCGGTTTGGCACGGAATAG

Upstream 100 bases:

>100_bases
GCGGATGATCAGCAGCTGATCAGCAGCTATCCGGTTGATCCGATCTGCGGCGGCACCGGAATGCGTTGTACCTTTGTAAA
ACTGGTAAAGGGGGCCTGAT

Downstream 100 bases:

>100_bases
GGGGGAGCATCTATGGAACTGACTATTACCGGCGCCAACGCCATGACCAATCCGGTCCTGCATATCTGGGACTGGCGTGT
TTCGCTCTACCTGTTTCTGG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MPKYAMVIDQRRCIGCMACIVACKAENNVPPEQFRTRVLEKTEGQFPNLRTELRSELCNHCDNPPCVYNCPTRASYKSRL
SGLVLLDKKRCVGCKACVAACPYDARYIDHNKGFADKCTFCDHRIKEGKEPACVATCIGKSRIFGDLNDPKSPVRLALKG
HHAELPMKSAGTEPRVYYIRRFGTE

Sequences:

>Translated_185_residues
MPKYAMVIDQRRCIGCMACIVACKAENNVPPEQFRTRVLEKTEGQFPNLRTELRSELCNHCDNPPCVYNCPTRASYKSRL
SGLVLLDKKRCVGCKACVAACPYDARYIDHNKGFADKCTFCDHRIKEGKEPACVATCIGKSRIFGDLNDPKSPVRLALKG
HHAELPMKSAGTEPRVYYIRRFGTE
>Mature_184_residues
PKYAMVIDQRRCIGCMACIVACKAENNVPPEQFRTRVLEKTEGQFPNLRTELRSELCNHCDNPPCVYNCPTRASYKSRLS
GLVLLDKKRCVGCKACVAACPYDARYIDHNKGFADKCTFCDHRIKEGKEPACVATCIGKSRIFGDLNDPKSPVRLALKGH
HAELPMKSAGTEPRVYYIRRFGTE

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=179, Percent_Identity=39.6648044692737, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI226510944, Length=188, Percent_Identity=35.1063829787234, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787872, Length=155, Percent_Identity=36.7741935483871, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1787122, Length=186, Percent_Identity=32.7956989247312, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787740, Length=104, Percent_Identity=41.3461538461538, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1787478, Length=103, Percent_Identity=40.7766990291262, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787749, Length=158, Percent_Identity=31.6455696202532, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1790326, Length=159, Percent_Identity=32.0754716981132, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1789370, Length=214, Percent_Identity=29.4392523364486, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082180, Length=130, Percent_Identity=35.3846153846154, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789067, Length=165, Percent_Identity=34.5454545454545, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367245, Length=145, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI87082179, Length=139, Percent_Identity=32.3741007194245, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788811, Length=149, Percent_Identity=29.5302013422819, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787963, Length=130, Percent_Identity=26.9230769230769, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20807; Mature: 20676

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
8.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
10.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYAMVIDQRRCIGCMACIVACKAENNVPPEQFRTRVLEKTEGQFPNLRTELRSELCNH
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CDNPPCVYNCPTRASYKSRLSGLVLLDKKRCVGCKACVAACPYDARYIDHNKGFADKCTF
CCCCCEEEECCCCCCHHHHCCCEEEECCCHHCCHHHHHHHCCCCCEEEECCCCCCHHHHH
CDHRIKEGKEPACVATCIGKSRIFGDLNDPKSPVRLALKGHHAELPMKSAGTEPRVYYIR
HHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCHHHCCCCCEEEEEE
RFGTE
ECCCC
>Mature Secondary Structure 
PKYAMVIDQRRCIGCMACIVACKAENNVPPEQFRTRVLEKTEGQFPNLRTELRSELCNH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
CDNPPCVYNCPTRASYKSRLSGLVLLDKKRCVGCKACVAACPYDARYIDHNKGFADKCTF
CCCCCEEEECCCCCCHHHHCCCEEEECCCHHCCHHHHHHHCCCCCEEEECCCCCCHHHHH
CDHRIKEGKEPACVATCIGKSRIFGDLNDPKSPVRLALKGHHAELPMKSAGTEPRVYYIR
HHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCCCCHHHCCCCCEEEEEE
RFGTE
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]