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The map label for this gene is amiC [H]

Identifier: 189424209

GI number: 189424209

Start: 1193664

End: 1194947

Strand: Reverse

Name: amiC [H]

Synonym: Glov_1144

Alternate gene names: 189424209

Gene position: 1194947-1193664 (Counterclockwise)

Preceding gene: 189424210

Following gene: 189424208

Centisome position: 30.5

GC content: 55.76

Gene sequence:

>1284_bases
ATGACGATCCGACCAAGACCGATTATATACCTGTTACTGGCGTTGTTTGCACTGACAACCGCTACCTCACCTGTCCACGC
CGCCAGAACAGCCAGAAAACAATCCACTGCAGAAAACAGAATAGCACCACCGGCCCAGGTACTGGACCTGAAATACTGGT
CAAACCCTGACTATACCCGGATTGCCATTACCCTTGACCGCGATGTGACCTGGGAAAGCCACGAACTGGACAAGTCTGCC
GGTCGTAGTGGCAAACCGGGCCGGATCTTCATTGACCTTCACAACGCCCGGCTGGGCAACAGCATGCGTGATATCACGAT
CGGCGACGGCCTTTTGAAAGGCGTCCGGACTGCACTGTACAAGCCGGGAGTAGTACGGGTGGTACTGGACACCGACAACA
TCAGGGATTACAAGATATTTCCCCTTTCAGACCCGGCTCGCCTGATTATCGATGTCAGAGGTGACCGGAGAACCGAGATC
AGCCGGCTGGAGAGTTCCCTGGCCAGCCACAGCCTGGACGGCGTAGCTGCGACAGCCCCCGCCAGGACCGCTACGGAGGA
GCCAAAGGCAGAACGGACTCCCAGATCAACCAAAAAGCACCCTTCCATCTCCAAGATCCGCCGGATTGTGGTGGATCCGG
GTCATGGCGGCCACGACCCCGGTGCAGTAGGTCAAAGCGGCCTGCAGGAAAAGGATGTGGTCTTGTCCATTGGCTTGAAA
CTGAGAGAAAAATTGCGGGAAGAGCTGGGGCTGGATGTGGTCATGACCCGCTCGACCGACATCTTTATTCCGCTGGAAGA
ACGGACCGCCATTGCCAACAAGGTCAACGCCGACCTGTTTGTCTCAATCCACGCCAATGCCGCCCCCAATCGAAACGCTG
CCGGTATCGAGACCTACTACCTGAATCTGGCCAAAACCGAGAAGGTGGCCCAACTGGCTGCCAAGGAAAACGGCACCTCG
CTGGAAAAGGTCAGCACGCTGCAGGCGATCCTGTTTGACCTGATGGCCAATTACAAACTGAATGACTCGGCCCATCTTGC
GGAAGAGGTGCAGAAGGCGCTCTACAAAACCAGCAGGTCAAAATACCCTGACACCAAAAATCTGGGTGTCAAGCAGGGCC
CTTTCTATGTGCTGGTCGGTGCCAGCATGCCCAGCATCCTGACAGAGGTTGCCTTTATCAGCAACAGTACCGAAGAGAGC
CATCTGAAGGACCCATCCTTTCATGATCTGGCTGCCGACGGCATCCTGGATGGTATCCGGGGCTATATCAGCTCGCTGAA
ATAA

Upstream 100 bases:

>100_bases
AACAGGGAGATCTGCTGCGTGAACGGTTGAAAAAATTGAATATTTCTGTTATGACACCGTTGGAAGCATTGAATCTGCTT
GATGAATTGAAGAAAATGGC

Downstream 100 bases:

>100_bases
TGACCGGAACCCTCTACATTGTGGCCACACCGATCGGTAATCTGGAGGATATGACCTTTCGGGCTGTCCGTATTCTCCAG
GAAGTTGACCTGATCGCCGC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTIRPRPIIYLLLALFALTTATSPVHAARTARKQSTAENRIAPPAQVLDLKYWSNPDYTRIAITLDRDVTWESHELDKSA
GRSGKPGRIFIDLHNARLGNSMRDITIGDGLLKGVRTALYKPGVVRVVLDTDNIRDYKIFPLSDPARLIIDVRGDRRTEI
SRLESSLASHSLDGVAATAPARTATEEPKAERTPRSTKKHPSISKIRRIVVDPGHGGHDPGAVGQSGLQEKDVVLSIGLK
LREKLREELGLDVVMTRSTDIFIPLEERTAIANKVNADLFVSIHANAAPNRNAAGIETYYLNLAKTEKVAQLAAKENGTS
LEKVSTLQAILFDLMANYKLNDSAHLAEEVQKALYKTSRSKYPDTKNLGVKQGPFYVLVGASMPSILTEVAFISNSTEES
HLKDPSFHDLAADGILDGIRGYISSLK

Sequences:

>Translated_427_residues
MTIRPRPIIYLLLALFALTTATSPVHAARTARKQSTAENRIAPPAQVLDLKYWSNPDYTRIAITLDRDVTWESHELDKSA
GRSGKPGRIFIDLHNARLGNSMRDITIGDGLLKGVRTALYKPGVVRVVLDTDNIRDYKIFPLSDPARLIIDVRGDRRTEI
SRLESSLASHSLDGVAATAPARTATEEPKAERTPRSTKKHPSISKIRRIVVDPGHGGHDPGAVGQSGLQEKDVVLSIGLK
LREKLREELGLDVVMTRSTDIFIPLEERTAIANKVNADLFVSIHANAAPNRNAAGIETYYLNLAKTEKVAQLAAKENGTS
LEKVSTLQAILFDLMANYKLNDSAHLAEEVQKALYKTSRSKYPDTKNLGVKQGPFYVLVGASMPSILTEVAFISNSTEES
HLKDPSFHDLAADGILDGIRGYISSLK
>Mature_426_residues
TIRPRPIIYLLLALFALTTATSPVHAARTARKQSTAENRIAPPAQVLDLKYWSNPDYTRIAITLDRDVTWESHELDKSAG
RSGKPGRIFIDLHNARLGNSMRDITIGDGLLKGVRTALYKPGVVRVVLDTDNIRDYKIFPLSDPARLIIDVRGDRRTEIS
RLESSLASHSLDGVAATAPARTATEEPKAERTPRSTKKHPSISKIRRIVVDPGHGGHDPGAVGQSGLQEKDVVLSIGLKL
REKLREELGLDVVMTRSTDIFIPLEERTAIANKVNADLFVSIHANAAPNRNAAGIETYYLNLAKTEKVAQLAAKENGTSL
EKVSTLQAILFDLMANYKLNDSAHLAEEVQKALYKTSRSKYPDTKNLGVKQGPFYVLVGASMPSILTEVAFISNSTEESH
LKDPSFHDLAADGILDGIRGYISSLK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=410, Percent_Identity=30, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788776, Length=270, Percent_Identity=34.0740740740741, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790611, Length=231, Percent_Identity=34.1991341991342, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46843; Mature: 46712

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRPRPIIYLLLALFALTTATSPVHAARTARKQSTAENRIAPPAQVLDLKYWSNPDYTR
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCEEE
IAITLDRDVTWESHELDKSAGRSGKPGRIFIDLHNARLGNSMRDITIGDGLLKGVRTALY
EEEEECCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHC
KPGVVRVVLDTDNIRDYKIFPLSDPARLIIDVRGDRRTEISRLESSLASHSLDGVAATAP
CCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECCC
ARTATEEPKAERTPRSTKKHPSISKIRRIVVDPGHGGHDPGAVGQSGLQEKDVVLSIGLK
CCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHH
LREKLREELGLDVVMTRSTDIFIPLEERTAIANKVNADLFVSIHANAAPNRNAAGIETYY
HHHHHHHHCCCEEEEECCCEEEEECHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEE
LNLAKTEKVAQLAAKENGTSLEKVSTLQAILFDLMANYKLNDSAHLAEEVQKALYKTSRS
EEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
KYPDTKNLGVKQGPFYVLVGASMPSILTEVAFISNSTEESHLKDPSFHDLAADGILDGIR
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHH
GYISSLK
HHHHHCC
>Mature Secondary Structure 
TIRPRPIIYLLLALFALTTATSPVHAARTARKQSTAENRIAPPAQVLDLKYWSNPDYTR
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCEEE
IAITLDRDVTWESHELDKSAGRSGKPGRIFIDLHNARLGNSMRDITIGDGLLKGVRTALY
EEEEECCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHC
KPGVVRVVLDTDNIRDYKIFPLSDPARLIIDVRGDRRTEISRLESSLASHSLDGVAATAP
CCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECCC
ARTATEEPKAERTPRSTKKHPSISKIRRIVVDPGHGGHDPGAVGQSGLQEKDVVLSIGLK
CCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHH
LREKLREELGLDVVMTRSTDIFIPLEERTAIANKVNADLFVSIHANAAPNRNAAGIETYY
HHHHHHHHCCCEEEEECCCEEEEECHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEE
LNLAKTEKVAQLAAKENGTSLEKVSTLQAILFDLMANYKLNDSAHLAEEVQKALYKTSRS
EEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
KYPDTKNLGVKQGPFYVLVGASMPSILTEVAFISNSTEESHLKDPSFHDLAADGILDGIR
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHH
GYISSLK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]