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The map label for this gene is intA [H]

Identifier: 189424200

GI number: 189424200

Start: 1185368

End: 1186630

Strand: Reverse

Name: intA [H]

Synonym: Glov_1135

Alternate gene names: 189424200

Gene position: 1186630-1185368 (Counterclockwise)

Preceding gene: 189424201

Following gene: 189424199

Centisome position: 30.29

GC content: 52.73

Gene sequence:

>1263_bases
ATGCCTAAAAGGATTGTACCCCTCTCCGACATTCAAGTTTCCAAGGCCAAACCCAAAGACAAAGACTACAATCTATTTGA
CGGTGGCGGCCTCTACCTACTCGTTACATCAACAGGCGGCAAACTCTGGCGGATGAAATACCGCTTTGAAGGTAAAGAGC
GCCGGATTGCATTTGGATCTTATCCTGAGATCTCTTTGGCAGATGCAAGAAGGCGGAAAGAGGAAGCCCGGAAGCTCGTC
GCAAACGGTACTGATCCGGGAAATCTAAAGAAGGCACAAAAGCAAGCCGGTGAAGACAGGACAGCAAACAGTTTTGAGGT
AATAGCACGGGAATGGTTTGGAGCCTTTCAGAACCAATGGACTCCCGGCCACGCCACCAAGCTCTTTAGCCGGCTGGAAA
GGGAACTGTTCCCCTATATCGGGGCAATGCCGATTGAAGAGATTAAGGCCCCGGACGTGCTGAAGGTTCTGAAACGGATT
GAATCACGAGGGATACTTGAGACCGCTCACCGGGTAAAGACGATCATCAGCCAGGTATTACGCTACGCCATAGCCCACGG
ACACCGGGCAGACCGTGATTGTACAGCAGACTTAAGGGGAGCATTACCCCCGGCAAGAGCAAAGCACTTCGGGGCACTGA
CAGACCCGAAAGAAGTTGCCCCCCTGTTAAGAGCCATAGACAGCTTTCAGGGAAGTTTTGTAGTCAAGTGCGCCTTACAG
CTTTCCCCTATGCTGTTCTGCCGCCCTGGGGAACTACGGGGCATGGAGTGGGCAGAGCTTAACCTTGATTCTGCCGAATG
GAACATACCCGTTGAAAGAATGAAGCTGAAGAACCAGACCAAGCAAGACCGCAAGGGGCAGTTCCACCTTGTCCCCCTCT
CCTCTCAAGCCGTCCAGATACTTCGAGAGCTGCACCCCCTGACTGGACACAGCAAATATGTTTTCCCCTGCCACCGCACC
CCATTACGACCTATGAGTGAAAACGCCATTACTGCAGCCTTGCGCCGTCTTGGGTATACCGGAAGCGAAATGACCGGTCA
CGGATTCAGGGCAACCGCCCGAACCATTCTGGATGAAGTATTAGGGTTCCGGCCTGACATAATTGAACACCAGCTAGCCC
ACGCGGTAAGGGATGCAAACGGCAGGGCCTACAATCGAACCTCTTTCCTTAAAGATCGGCGTCAGATGATGCAGCAATGG
GCTGACTACCTGGACGGATTAAAGGCCGGGGCGAAGGTTATTTCGATCAGACAGGTGGTGTAA

Upstream 100 bases:

>100_bases
AGTCTATATTGATATGCTTGAATCCACGTTTTTTGGGGATATATTTTCAGCAAAATATCCCCATAGCATTTTGGGTATCC
CCAAAACGGAGGAAAGCGCC

Downstream 100 bases:

>100_bases
CCGGGGATAAATACAGAGGCTTGATTTATACTTCTGAAAGGTATATTTTATGACTTGGACAGTTAAGCTGTCACGAGCAG
CAGAAAAGCAAAAAAGCAAA

Product: integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPKRIVPLSDIQVSKAKPKDKDYNLFDGGGLYLLVTSTGGKLWRMKYRFEGKERRIAFGSYPEISLADARRRKEEARKLV
ANGTDPGNLKKAQKQAGEDRTANSFEVIAREWFGAFQNQWTPGHATKLFSRLERELFPYIGAMPIEEIKAPDVLKVLKRI
ESRGILETAHRVKTIISQVLRYAIAHGHRADRDCTADLRGALPPARAKHFGALTDPKEVAPLLRAIDSFQGSFVVKCALQ
LSPMLFCRPGELRGMEWAELNLDSAEWNIPVERMKLKNQTKQDRKGQFHLVPLSSQAVQILRELHPLTGHSKYVFPCHRT
PLRPMSENAITAALRRLGYTGSEMTGHGFRATARTILDEVLGFRPDIIEHQLAHAVRDANGRAYNRTSFLKDRRQMMQQW
ADYLDGLKAGAKVISIRQVV

Sequences:

>Translated_420_residues
MPKRIVPLSDIQVSKAKPKDKDYNLFDGGGLYLLVTSTGGKLWRMKYRFEGKERRIAFGSYPEISLADARRRKEEARKLV
ANGTDPGNLKKAQKQAGEDRTANSFEVIAREWFGAFQNQWTPGHATKLFSRLERELFPYIGAMPIEEIKAPDVLKVLKRI
ESRGILETAHRVKTIISQVLRYAIAHGHRADRDCTADLRGALPPARAKHFGALTDPKEVAPLLRAIDSFQGSFVVKCALQ
LSPMLFCRPGELRGMEWAELNLDSAEWNIPVERMKLKNQTKQDRKGQFHLVPLSSQAVQILRELHPLTGHSKYVFPCHRT
PLRPMSENAITAALRRLGYTGSEMTGHGFRATARTILDEVLGFRPDIIEHQLAHAVRDANGRAYNRTSFLKDRRQMMQQW
ADYLDGLKAGAKVISIRQVV
>Mature_419_residues
PKRIVPLSDIQVSKAKPKDKDYNLFDGGGLYLLVTSTGGKLWRMKYRFEGKERRIAFGSYPEISLADARRRKEEARKLVA
NGTDPGNLKKAQKQAGEDRTANSFEVIAREWFGAFQNQWTPGHATKLFSRLERELFPYIGAMPIEEIKAPDVLKVLKRIE
SRGILETAHRVKTIISQVLRYAIAHGHRADRDCTADLRGALPPARAKHFGALTDPKEVAPLLRAIDSFQGSFVVKCALQL
SPMLFCRPGELRGMEWAELNLDSAEWNIPVERMKLKNQTKQDRKGQFHLVPLSSQAVQILRELHPLTGHSKYVFPCHRTP
LRPMSENAITAALRRLGYTGSEMTGHGFRATARTILDEVLGFRPDIIEHQLAHAVRDANGRAYNRTSFLKDRRQMMQQWA
DYLDGLKAGAKVISIRQVV

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=417, Percent_Identity=35.0119904076739, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1788690, Length=396, Percent_Identity=35.8585858585859, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI145693166, Length=407, Percent_Identity=36.1179361179361, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47560; Mature: 47428

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKRIVPLSDIQVSKAKPKDKDYNLFDGGGLYLLVTSTGGKLWRMKYRFEGKERRIAFGS
CCCCCCCCCCCEECCCCCCCCCCCEECCCCEEEEEECCCCCEEEEEEEECCCCCEEEECC
YPEISLADARRRKEEARKLVANGTDPGNLKKAQKQAGEDRTANSFEVIAREWFGAFQNQW
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
TPGHATKLFSRLERELFPYIGAMPIEEIKAPDVLKVLKRIESRGILETAHRVKTIISQVL
CCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RYAIAHGHRADRDCTADLRGALPPARAKHFGALTDPKEVAPLLRAIDSFQGSFVVKCALQ
HHHHHCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHH
LSPMLFCRPGELRGMEWAELNLDSAEWNIPVERMKLKNQTKQDRKGQFHLVPLSSQAVQI
CCCEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
LRELHPLTGHSKYVFPCHRTPLRPMSENAITAALRRLGYTGSEMTGHGFRATARTILDEV
HHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGFRPDIIEHQLAHAVRDANGRAYNRTSFLKDRRQMMQQWADYLDGLKAGAKVISIRQVV
HCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
PKRIVPLSDIQVSKAKPKDKDYNLFDGGGLYLLVTSTGGKLWRMKYRFEGKERRIAFGS
CCCCCCCCCCEECCCCCCCCCCCEECCCCEEEEEECCCCCEEEEEEEECCCCCEEEECC
YPEISLADARRRKEEARKLVANGTDPGNLKKAQKQAGEDRTANSFEVIAREWFGAFQNQW
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
TPGHATKLFSRLERELFPYIGAMPIEEIKAPDVLKVLKRIESRGILETAHRVKTIISQVL
CCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RYAIAHGHRADRDCTADLRGALPPARAKHFGALTDPKEVAPLLRAIDSFQGSFVVKCALQ
HHHHHCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHH
LSPMLFCRPGELRGMEWAELNLDSAEWNIPVERMKLKNQTKQDRKGQFHLVPLSSQAVQI
CCCEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
LRELHPLTGHSKYVFPCHRTPLRPMSENAITAALRRLGYTGSEMTGHGFRATARTILDEV
HHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
LGFRPDIIEHQLAHAVRDANGRAYNRTSFLKDRRQMMQQWADYLDGLKAGAKVISIRQVV
HCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]