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The map label for this gene is hldE

Identifier: 189424043

GI number: 189424043

Start: 1015565

End: 1017040

Strand: Reverse

Name: hldE

Synonym: Glov_0977

Alternate gene names: 189424043

Gene position: 1017040-1015565 (Counterclockwise)

Preceding gene: 189424044

Following gene: 189424041

Centisome position: 25.96

GC content: 61.25

Gene sequence:

>1476_bases
ATGGATCGTAAAATGGTCGAATCACTGTTTCAGCGCGCCTCCACCCTGAAATGTCTGGTTGTCGGCGACCTGATGCTGGA
TGAATACCTCTGGGGCAGGACCGACCGGATCTCTCCCGAGGCCCCGGTACAGGTGGTGGATGTGCTGCGGGAAGACCTGC
GCCTGGGCGGGGCCGGCAACGTGGCCAACAACCTGCTGGCGCTGGGCTGTCAGGTCACGGTCGCCTCGGTGATCGGCGAG
GACGAGAACGGTTGGGCACTACTGAAGGCATTCAGCCGGCAGGGGGTTGATACCGCCCCGATTTATCAGGAGCCGGGACG
CCGTACCGGCCGCAAAACACGGGTGATTGCCGCCAACCAGCAGATCGTCAGGATTGACCGGGAATCCCGCGAGCCGCTCT
CCGGGCAGATCGAACAACAGCTGATCAACTGGCTGCAGCAACATATAACCGGTTTTGATGTAGTGCTGGTATCCGATTAT
CTGAAAGGGGTGCTGACACCGTCCGTCCTGGCCTCTGTCACCTCCACGGCAAGCAGGCGCAACATTCCTGTTCTGGTAGA
CCCCAAAGGCAGTGACTACAGCAAGTACCGGGGCGCCACCTGTCTGACCCCCAACCGCAAGGAGGCCGAAGCTGCTTCCG
GGGTACCGATCCAAGACGGTGCCAGCCTGCAGCAGGCTGCCGACACGATCATGTCCACCGTGGGACTGGATAACCTGTTG
ATCACCCGCAGCGAAGAAGGGATGTCACTTTTCTGCGGCAATGGCGAGACCGTCCATATCCCGACCGTTGCCCGCGAGGT
ATTTGATGTCACCGGTGCCGGCGACACCGTGCTGGCCCTGCTGGCCTGCGGGCTTGCCGGCGGCCTGCCCTTGGCCGAGT
CGGCCCGGCTGGCCAACGTTGCCGCCGGGATCGCCGTGGCCAAACTGGGCACCTCCACCGTCACCCCGGCCGAGATTATC
GCTGCGGTCTCCCTGGAACACCGCGACAGTGACAGCAAGATCAAGAACCGTGAGGTACTGTCAGAGATCATTGCCGCGGA
ACGGGCCAAGGGCAGGCAGGTGGTCTTTACCAACGGCTGTTTCGACCTGCTGCATGCCGGACATGTCAAATACCTGCAGG
CCGCCCGCCGCCTGGGAGACCTGCTGATTCTGGGACTGAACAGCGATGCCTCGGTGCGGCGCCTGAAAGGGCCCAAACGC
CCGCTGATTGACGAAGATGAACGGGGCCACCTGATGGCTGCCCTGGACTGTATCGACTACGTCTGTCTGTTTGAGGAGGA
CACCCCGCTGGAACTGATTACCGCCCTGAAACCGCAGATTCTGGTCAAAGGTGGCGACTACACGCCGGAGGGGGTCGTTG
GCAAGGATCTGGTGGAGTCCTACGGAGGCAGGGTCGAACTGATCCCCTTTGTGGATGGCAAATCAACCACCAACATCATC
GAAAAGGTACTGGAACGCTACACCGACGAGCAGTAA

Upstream 100 bases:

>100_bases
CCCCCGGGTACAGGAAGGTCACATCGCCGTAATCCACATCCTCTGCGACCTGATCGAGCAGGGCCTGTTCGGCATATTTG
CCGGGGAAGGACGTTAGACC

Downstream 100 bases:

>100_bases
AAGAAAAGGGCGGCCTGGTTGGCCGCCCTTTTCTTTTACGCCTATCCCCACTCAGCAGGCCACTGCGAAACGTTACCCTA
AAGCAGGACAACCAGTCCAC

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MDRKMVESLFQRASTLKCLVVGDLMLDEYLWGRTDRISPEAPVQVVDVLREDLRLGGAGNVANNLLALGCQVTVASVIGE
DENGWALLKAFSRQGVDTAPIYQEPGRRTGRKTRVIAANQQIVRIDRESREPLSGQIEQQLINWLQQHITGFDVVLVSDY
LKGVLTPSVLASVTSTASRRNIPVLVDPKGSDYSKYRGATCLTPNRKEAEAASGVPIQDGASLQQAADTIMSTVGLDNLL
ITRSEEGMSLFCGNGETVHIPTVAREVFDVTGAGDTVLALLACGLAGGLPLAESARLANVAAGIAVAKLGTSTVTPAEII
AAVSLEHRDSDSKIKNREVLSEIIAAERAKGRQVVFTNGCFDLLHAGHVKYLQAARRLGDLLILGLNSDASVRRLKGPKR
PLIDEDERGHLMAALDCIDYVCLFEEDTPLELITALKPQILVKGGDYTPEGVVGKDLVESYGGRVELIPFVDGKSTTNII
EKVLERYTDEQ

Sequences:

>Translated_491_residues
MDRKMVESLFQRASTLKCLVVGDLMLDEYLWGRTDRISPEAPVQVVDVLREDLRLGGAGNVANNLLALGCQVTVASVIGE
DENGWALLKAFSRQGVDTAPIYQEPGRRTGRKTRVIAANQQIVRIDRESREPLSGQIEQQLINWLQQHITGFDVVLVSDY
LKGVLTPSVLASVTSTASRRNIPVLVDPKGSDYSKYRGATCLTPNRKEAEAASGVPIQDGASLQQAADTIMSTVGLDNLL
ITRSEEGMSLFCGNGETVHIPTVAREVFDVTGAGDTVLALLACGLAGGLPLAESARLANVAAGIAVAKLGTSTVTPAEII
AAVSLEHRDSDSKIKNREVLSEIIAAERAKGRQVVFTNGCFDLLHAGHVKYLQAARRLGDLLILGLNSDASVRRLKGPKR
PLIDEDERGHLMAALDCIDYVCLFEEDTPLELITALKPQILVKGGDYTPEGVVGKDLVESYGGRVELIPFVDGKSTTNII
EKVLERYTDEQ
>Mature_491_residues
MDRKMVESLFQRASTLKCLVVGDLMLDEYLWGRTDRISPEAPVQVVDVLREDLRLGGAGNVANNLLALGCQVTVASVIGE
DENGWALLKAFSRQGVDTAPIYQEPGRRTGRKTRVIAANQQIVRIDRESREPLSGQIEQQLINWLQQHITGFDVVLVSDY
LKGVLTPSVLASVTSTASRRNIPVLVDPKGSDYSKYRGATCLTPNRKEAEAASGVPIQDGASLQQAADTIMSTVGLDNLL
ITRSEEGMSLFCGNGETVHIPTVAREVFDVTGAGDTVLALLACGLAGGLPLAESARLANVAAGIAVAKLGTSTVTPAEII
AAVSLEHRDSDSKIKNREVLSEIIAAERAKGRQVVFTNGCFDLLHAGHVKYLQAARRLGDLLILGLNSDASVRRLKGPKR
PLIDEDERGHLMAALDCIDYVCLFEEDTPLELITALKPQILVKGGDYTPEGVVGKDLVESYGGRVELIPFVDGKSTTNII
EKVLERYTDEQ

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Homo sapiens, GI4505651, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI296841136, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789432, Length=474, Percent_Identity=44.7257383966245, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788491, Length=261, Percent_Identity=27.9693486590038, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550974, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550973, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45550975, Length=133, Percent_Identity=32.3308270676692, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_GEOLS (B3E5M9)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951220.1
- ProteinModelPortal:   B3E5M9
- SMR:   B3E5M9
- GeneID:   6366825
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0977
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   CLSK828725
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 53022; Mature: 53022

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKMVESLFQRASTLKCLVVGDLMLDEYLWGRTDRISPEAPVQVVDVLREDLRLGGAGN
CCHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCH
VANNLLALGCQVTVASVIGEDENGWALLKAFSRQGVDTAPIYQEPGRRTGRKTRVIAANQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCC
QIVRIDRESREPLSGQIEQQLINWLQQHITGFDVVLVSDYLKGVLTPSVLASVTSTASRR
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NIPVLVDPKGSDYSKYRGATCLTPNRKEAEAASGVPIQDGASLQQAADTIMSTVGLDNLL
CCCEEECCCCCCHHHHCCCEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
ITRSEEGMSLFCGNGETVHIPTVAREVFDVTGAGDTVLALLACGLAGGLPLAESARLANV
EEECCCCCEEEECCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
AAGIAVAKLGTSTVTPAEIIAAVSLEHRDSDSKIKNREVLSEIIAAERAKGRQVVFTNGC
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCH
FDLLHAGHVKYLQAARRLGDLLILGLNSDASVRRLKGPKRPLIDEDERGHLMAALDCIDY
HHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VCLFEEDTPLELITALKPQILVKGGDYTPEGVVGKDLVESYGGRVELIPFVDGKSTTNII
HEEECCCCCHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHH
EKVLERYTDEQ
HHHHHHHCCCC
>Mature Secondary Structure
MDRKMVESLFQRASTLKCLVVGDLMLDEYLWGRTDRISPEAPVQVVDVLREDLRLGGAGN
CCHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCH
VANNLLALGCQVTVASVIGEDENGWALLKAFSRQGVDTAPIYQEPGRRTGRKTRVIAANQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCC
QIVRIDRESREPLSGQIEQQLINWLQQHITGFDVVLVSDYLKGVLTPSVLASVTSTASRR
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NIPVLVDPKGSDYSKYRGATCLTPNRKEAEAASGVPIQDGASLQQAADTIMSTVGLDNLL
CCCEEECCCCCCHHHHCCCEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
ITRSEEGMSLFCGNGETVHIPTVAREVFDVTGAGDTVLALLACGLAGGLPLAESARLANV
EEECCCCCEEEECCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
AAGIAVAKLGTSTVTPAEIIAAVSLEHRDSDSKIKNREVLSEIIAAERAKGRQVVFTNGC
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCH
FDLLHAGHVKYLQAARRLGDLLILGLNSDASVRRLKGPKRPLIDEDERGHLMAALDCIDY
HHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VCLFEEDTPLELITALKPQILVKGGDYTPEGVVGKDLVESYGGRVELIPFVDGKSTTNII
HEEECCCCCHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHH
EKVLERYTDEQ
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA