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The map label for this gene is selU [H]

Identifier: 189424014

GI number: 189424014

Start: 984443

End: 985459

Strand: Reverse

Name: selU [H]

Synonym: Glov_0948

Alternate gene names: 189424014

Gene position: 985459-984443 (Counterclockwise)

Preceding gene: 189424018

Following gene: 189424012

Centisome position: 25.15

GC content: 59.59

Gene sequence:

>1017_bases
ATGGCCCGCACGATCCCGTTTGATCCCTCACTGTTTGAAACCCACTGTATTATTGATGCCCGCACGCCGCTGGAGTTTGC
AGAAGACCATCTGCCCGGCGCCATCAATGTGCCGATCCTGACCGATGCTGAACGGGTGGAGATCGGCACCCTCTACAAGC
AGCAGGGGCCGCAACCGGCCCGTGTCCGCGGCCTGGAACTGATCTGCCACCGCTTTCCCGCCATTGTTGCCACCATTGCT
GAAGCAGCCAAGGGACGACCGGTGCTGGTCTACTGCTGGCGAGGCGGCCTGCGCAGCGAATCAATCGCCCTGCTGCTGGA
GATGACCGGCTACCCGGTGGTCAAACTGGCTGGCGGCTATAAATCGTTCCGTACCATCGTCACCTCCTTCTTTGCATCGG
TCAGCCTGCCGGTCCAGTTGGTGGTGCTGCACGGCATGACCGGATCAGGCAAGACCGAATTCCTGCAGCAACTGCCGCAG
GAACGGTACACCGTGATTGATCTTGAAGGGTTGGCCAGACACCGGGGCTCGGCCTTTGGCTCGTTGGGGCTGGGAGAACA
ACCGCCCCAGAAGCGGTTTGAGACCCTGCTCTGGGACGCCTTCCGCAAGGCCCCTACAGATCGGCCGATTGTGGTGGAAG
GGGAAAGCAAACGGATCGGCCGGGTGACCCTGCCCGGCGACCTGTATGAGGTTATGGCAGCCGGCACCAAGGTCTGGTGT
GAGGTGTCAGTGGCAACCAGGGTCAAGCGCCTGTCTGCCGAATATGCGATGGAGGAATACCGCCAGCCGATGGCAGAGGC
GTTGGAGCGGATCAAGAAAAAACTGGGGGGGCCGCAGTACACAGAGCTGCAGAACAAACTTGCAGCCTGGGATATTCCGG
GGGTGGCCCAGGATCTGATCGAGCAGTACTACGACAAGCTCTATTACCGGGTCAGAAAATGGGAGCCGGAGGGGACCATC
AATCTTGAAGATTATGCTGCTGCAGAAAAGGAACTGGCGGAAATCTGCTGCATTTGA

Upstream 100 bases:

>100_bases
TAACCTTGCAAATACGAGGGAAAGAGTGTTTATATGTACCACTGAACACCCTGCATGGCAAGGGTGATCCCAGAAAAAAC
CACCGGAGTATCTGACCACA

Downstream 100 bases:

>100_bases
TCCGGGACGAATGCCTCCCGCGCCCAGTTCAACAACTCTGCGCAGTTCGCCCCGCGCAGTGCATCCGGCACTGCCAACCC
AAGAAAATCAAGCGCCCAGA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MARTIPFDPSLFETHCIIDARTPLEFAEDHLPGAINVPILTDAERVEIGTLYKQQGPQPARVRGLELICHRFPAIVATIA
EAAKGRPVLVYCWRGGLRSESIALLLEMTGYPVVKLAGGYKSFRTIVTSFFASVSLPVQLVVLHGMTGSGKTEFLQQLPQ
ERYTVIDLEGLARHRGSAFGSLGLGEQPPQKRFETLLWDAFRKAPTDRPIVVEGESKRIGRVTLPGDLYEVMAAGTKVWC
EVSVATRVKRLSAEYAMEEYRQPMAEALERIKKKLGGPQYTELQNKLAAWDIPGVAQDLIEQYYDKLYYRVRKWEPEGTI
NLEDYAAAEKELAEICCI

Sequences:

>Translated_338_residues
MARTIPFDPSLFETHCIIDARTPLEFAEDHLPGAINVPILTDAERVEIGTLYKQQGPQPARVRGLELICHRFPAIVATIA
EAAKGRPVLVYCWRGGLRSESIALLLEMTGYPVVKLAGGYKSFRTIVTSFFASVSLPVQLVVLHGMTGSGKTEFLQQLPQ
ERYTVIDLEGLARHRGSAFGSLGLGEQPPQKRFETLLWDAFRKAPTDRPIVVEGESKRIGRVTLPGDLYEVMAAGTKVWC
EVSVATRVKRLSAEYAMEEYRQPMAEALERIKKKLGGPQYTELQNKLAAWDIPGVAQDLIEQYYDKLYYRVRKWEPEGTI
NLEDYAAAEKELAEICCI
>Mature_337_residues
ARTIPFDPSLFETHCIIDARTPLEFAEDHLPGAINVPILTDAERVEIGTLYKQQGPQPARVRGLELICHRFPAIVATIAE
AAKGRPVLVYCWRGGLRSESIALLLEMTGYPVVKLAGGYKSFRTIVTSFFASVSLPVQLVVLHGMTGSGKTEFLQQLPQE
RYTVIDLEGLARHRGSAFGSLGLGEQPPQKRFETLLWDAFRKAPTDRPIVVEGESKRIGRVTLPGDLYEVMAAGTKVWCE
VSVATRVKRLSAEYAMEEYRQPMAEALERIKKKLGGPQYTELQNKLAAWDIPGVAQDLIEQYYDKLYYRVRKWEPEGTIN
LEDYAAAEKELAEICCI

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=341, Percent_Identity=34.8973607038123, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 37845; Mature: 37714

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTIPFDPSLFETHCIIDARTPLEFAEDHLPGAINVPILTDAERVEIGTLYKQQGPQPA
CCCCCCCCCCCHHEEEEEECCCCHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHCCCCHH
RVRGLELICHRFPAIVATIAEAAKGRPVLVYCWRGGLRSESIALLLEMTGYPVVKLAGGY
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEECCCH
KSFRTIVTSFFASVSLPVQLVVLHGMTGSGKTEFLQQLPQERYTVIDLEGLARHRGSAFG
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCHHCEEEEEHHHHHHHCCCCCC
SLGLGEQPPQKRFETLLWDAFRKAPTDRPIVVEGESKRIGRVTLPGDLYEVMAAGTKVWC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHCCCCEEE
EVSVATRVKRLSAEYAMEEYRQPMAEALERIKKKLGGPQYTELQNKLAAWDIPGVAQDLI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHH
EQYYDKLYYRVRKWEPEGTINLEDYAAAEKELAEICCI
HHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARTIPFDPSLFETHCIIDARTPLEFAEDHLPGAINVPILTDAERVEIGTLYKQQGPQPA
CCCCCCCCCCHHEEEEEECCCCHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHCCCCHH
RVRGLELICHRFPAIVATIAEAAKGRPVLVYCWRGGLRSESIALLLEMTGYPVVKLAGGY
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEECCCH
KSFRTIVTSFFASVSLPVQLVVLHGMTGSGKTEFLQQLPQERYTVIDLEGLARHRGSAFG
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCHHCEEEEEHHHHHHHCCCCCC
SLGLGEQPPQKRFETLLWDAFRKAPTDRPIVVEGESKRIGRVTLPGDLYEVMAAGTKVWC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHCCCCEEE
EVSVATRVKRLSAEYAMEEYRQPMAEALERIKKKLGGPQYTELQNKLAAWDIPGVAQDLI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHH
EQYYDKLYYRVRKWEPEGTINLEDYAAAEKELAEICCI
HHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA