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The map label for this gene is phsA [H]

Identifier: 189424001

GI number: 189424001

Start: 966167

End: 968404

Strand: Reverse

Name: phsA [H]

Synonym: Glov_0935

Alternate gene names: 189424001

Gene position: 968404-966167 (Counterclockwise)

Preceding gene: 189424002

Following gene: 189424000

Centisome position: 24.72

GC content: 58.94

Gene sequence:

>2238_bases
ATGAGTAAAAACATTGACATAACGCAAGCTGGCGTTGAGGTAAAGAAGTCAGCTTGCTATTTTTGCCACCAGAACTGTGG
TGTGCTGGCCTATGTAAAAGACGGCAAGGTGCTCAAGATCGAAGGAGATCCGGAGTTCCCCACAAACCAGGGCGGTTTGT
GCTGCCGGGGCAATATCGCCCTGCAGCATCTTGATCATCCGGCGCGGATTAACTACCCCCTGAAGCGGGTTGGCAAGCGT
GGTGAAGGGAAATGGGAACAGATCCCCTGGGACCAGGCCATGAAGGAGATCGGTTCCAAACTGCTCCAGATCCGCAGTGA
GTTCGGTGCGGAGGCGGTGGCAACCGCCGGCGGCACCCAGCGTACCGACGACTGGTCCAGACGACGCTTCATGAACCTGT
TCGGCAGCCCCAACGGCTTCCATAACTCCCATCTCTGCTGGATCCCCACCTTTATGGTGGAAACGGCCATCTACGGCTGG
TGTCCGTTTGAACTGGACATGGGCAGCAGCCGCTGCCTGATCCTGTGGGGTCAAAACCCCGGTGCCGCCGGTATGCCGGA
GATGCACCACATCCGGGAGCTGCAGGAAAAAGGGATGAAGCTGATCGTGATCGACCCACGTTTCAGCGAAACCGCTGCCA
AGGCCGATCTCTGGCTGCCGCTGCGTCCCGGCTCCGACCTGGCGCTGGCACTGGCCTGGATCCATGTCATCATCTTTGAA
GGCCTGTACGACCAGGAGTTTGTCGCAAACTGCTGCGAAGGTTTCGGCGAACTGGCCGACCATGTTGAAAACTTCACGCC
GGAATGGGCAGCGGAACTGACCTGGCTGACCCCGGAACAGATCCGCGCAGGCGCCTACATGTATGCCATGAACAAACCGG
GCAACATCCAGTGGGGCACCTCGGTGGACCAGATCGGGAAACCGGCCGGCTCCACCATGCATGCCCGTGCGATCCTGCGG
GCCCTGACCGGCAACCTTGATGCCCCCGGTTCCGACCTGCTGACCGGCCCGAGCCCCGACTTCCTGACCGATGAAGAGAT
GGAGGCCAACGATCAGCTGCCGGAAGAGCAGAAGGCCAAGCAGATCGGCTCTGATCGTTTCAAACTGGTGACCTGGCCGG
GCTACAGCAAGATCGCTGAACAGACCAGAAAGGTCTGGGGCAAGGCCCCCACCGCCGAGTGGATGTGTGAGGCCCACCCT
CCCTCAGTCTTCAGATCGATCCTGAGCGGCAAACCGTATCAGGTCAAGGCGTTGCTGGTCTCGGCCACCAACCCGATCAA
CTCCTATGGAGAAACCAGTCTGGTGCTGGATGCGTTGAAGGCGGTCGACTTCATGGTCACCTGCGATTACTGGATGACAC
CGACCGCAGCCCTGTCCGATTACGTGATCCCGATTGCCGGTGCCCTTGAAAGACCGACCATCACCAGCAGCTACGGTTGT
TCCGACTTCCTGCTGGCATCACAACGGGCGATTCAGCCGATGTACGAACGTCGCAACGACTACAACTTCTGGCGTGATCT
GGGCTGTGCCATGGGCCAGCAGGAGATGTGGCCCTGGGCAACGGTTGAAGAGGCCTATTATGCCAAGATAGCCCCCCTTG
GCTACGACATCGAAAGCTACGATGAATTCGTTGAGCGCTACCGTTTCCACTTCCCCGAGCGTGAGTACTTCAAGTACCAG
CGCCAGGGTTTTGCAACCCCGTCGGGCAAGGTGGAGCTGTACTCAACGGTACTCAAGGATCTCGGCTATCCTCCGCTGCC
CCCCTATATCGGGCCCACCGAGAACGAGATCGACGACCCGGAACTGGCCAAGGAGTTTCCGCTGGTGCTGACCACCGGTG
GCGGATTCATGCCCTACCACCACTCCGAGCATTTCCAGATCAAGGAAGTACGCTTCCTGCGCCATGAGCCGTACATGGAT
ATCAACCCGGCAACCGCCGCCGAGCTGTCCATTGAAGATGGCGACTGGGTCTGGATCGAAACCAAGCGGGGACGTATCAA
GCAGAAGGCCAACCTGACCCAGGGGATCCATCCCCGTGTCATCTACACCCAGCGCGGCTGGTGGTATCCGGAGCGCAGCA
TGGCGGCGCCGGAGCTGGGCGGCTGTCTGGAATCAAACACCAACGTACTGACGACCACTGCCGATGATCACTGCGACCCC
TATAGCGGTTCCTGGGCTAACCGTGGTCTGCTCTGCCGCGTTTATAAAGTAAATGCTTCAGATATGGAGGGCAAATAA

Upstream 100 bases:

>100_bases
ATCAATAGGCACGAAAGCTGTGTGAACTGATGCATGTCTGGAACGTGAAGCACGACAGACGCAAACAATCTGTTCCGGTG
AAGCCAGTGAGGTTTATATT

Downstream 100 bases:

>100_bases
GATGGCTCGTTACGCGATGGTTATAGATACACGAAAATGCATCGGCTGCCATTCCTGTACGGTCGCCTGCAAGGTGCACA
ACGAACTGCCGGTTGAGATG

Product: molybdopterin oxidoreductase

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MSKNIDITQAGVEVKKSACYFCHQNCGVLAYVKDGKVLKIEGDPEFPTNQGGLCCRGNIALQHLDHPARINYPLKRVGKR
GEGKWEQIPWDQAMKEIGSKLLQIRSEFGAEAVATAGGTQRTDDWSRRRFMNLFGSPNGFHNSHLCWIPTFMVETAIYGW
CPFELDMGSSRCLILWGQNPGAAGMPEMHHIRELQEKGMKLIVIDPRFSETAAKADLWLPLRPGSDLALALAWIHVIIFE
GLYDQEFVANCCEGFGELADHVENFTPEWAAELTWLTPEQIRAGAYMYAMNKPGNIQWGTSVDQIGKPAGSTMHARAILR
ALTGNLDAPGSDLLTGPSPDFLTDEEMEANDQLPEEQKAKQIGSDRFKLVTWPGYSKIAEQTRKVWGKAPTAEWMCEAHP
PSVFRSILSGKPYQVKALLVSATNPINSYGETSLVLDALKAVDFMVTCDYWMTPTAALSDYVIPIAGALERPTITSSYGC
SDFLLASQRAIQPMYERRNDYNFWRDLGCAMGQQEMWPWATVEEAYYAKIAPLGYDIESYDEFVERYRFHFPEREYFKYQ
RQGFATPSGKVELYSTVLKDLGYPPLPPYIGPTENEIDDPELAKEFPLVLTTGGGFMPYHHSEHFQIKEVRFLRHEPYMD
INPATAAELSIEDGDWVWIETKRGRIKQKANLTQGIHPRVIYTQRGWWYPERSMAAPELGGCLESNTNVLTTTADDHCDP
YSGSWANRGLLCRVYKVNASDMEGK

Sequences:

>Translated_745_residues
MSKNIDITQAGVEVKKSACYFCHQNCGVLAYVKDGKVLKIEGDPEFPTNQGGLCCRGNIALQHLDHPARINYPLKRVGKR
GEGKWEQIPWDQAMKEIGSKLLQIRSEFGAEAVATAGGTQRTDDWSRRRFMNLFGSPNGFHNSHLCWIPTFMVETAIYGW
CPFELDMGSSRCLILWGQNPGAAGMPEMHHIRELQEKGMKLIVIDPRFSETAAKADLWLPLRPGSDLALALAWIHVIIFE
GLYDQEFVANCCEGFGELADHVENFTPEWAAELTWLTPEQIRAGAYMYAMNKPGNIQWGTSVDQIGKPAGSTMHARAILR
ALTGNLDAPGSDLLTGPSPDFLTDEEMEANDQLPEEQKAKQIGSDRFKLVTWPGYSKIAEQTRKVWGKAPTAEWMCEAHP
PSVFRSILSGKPYQVKALLVSATNPINSYGETSLVLDALKAVDFMVTCDYWMTPTAALSDYVIPIAGALERPTITSSYGC
SDFLLASQRAIQPMYERRNDYNFWRDLGCAMGQQEMWPWATVEEAYYAKIAPLGYDIESYDEFVERYRFHFPEREYFKYQ
RQGFATPSGKVELYSTVLKDLGYPPLPPYIGPTENEIDDPELAKEFPLVLTTGGGFMPYHHSEHFQIKEVRFLRHEPYMD
INPATAAELSIEDGDWVWIETKRGRIKQKANLTQGIHPRVIYTQRGWWYPERSMAAPELGGCLESNTNVLTTTADDHCDP
YSGSWANRGLLCRVYKVNASDMEGK
>Mature_744_residues
SKNIDITQAGVEVKKSACYFCHQNCGVLAYVKDGKVLKIEGDPEFPTNQGGLCCRGNIALQHLDHPARINYPLKRVGKRG
EGKWEQIPWDQAMKEIGSKLLQIRSEFGAEAVATAGGTQRTDDWSRRRFMNLFGSPNGFHNSHLCWIPTFMVETAIYGWC
PFELDMGSSRCLILWGQNPGAAGMPEMHHIRELQEKGMKLIVIDPRFSETAAKADLWLPLRPGSDLALALAWIHVIIFEG
LYDQEFVANCCEGFGELADHVENFTPEWAAELTWLTPEQIRAGAYMYAMNKPGNIQWGTSVDQIGKPAGSTMHARAILRA
LTGNLDAPGSDLLTGPSPDFLTDEEMEANDQLPEEQKAKQIGSDRFKLVTWPGYSKIAEQTRKVWGKAPTAEWMCEAHPP
SVFRSILSGKPYQVKALLVSATNPINSYGETSLVLDALKAVDFMVTCDYWMTPTAALSDYVIPIAGALERPTITSSYGCS
DFLLASQRAIQPMYERRNDYNFWRDLGCAMGQQEMWPWATVEEAYYAKIAPLGYDIESYDEFVERYRFHFPEREYFKYQR
QGFATPSGKVELYSTVLKDLGYPPLPPYIGPTENEIDDPELAKEFPLVLTTGGGFMPYHHSEHFQIKEVRFLRHEPYMDI
NPATAAELSIEDGDWVWIETKRGRIKQKANLTQGIHPRVIYTQRGWWYPERSMAAPELGGCLESNTNVLTTTADDHCDPY
SGSWANRGLLCRVYKVNASDMEGK

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=738, Percent_Identity=26.2872628726287, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787870, Length=782, Percent_Identity=28.3887468030691, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI87081797, Length=786, Percent_Identity=26.7175572519084, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI171474008, Length=767, Percent_Identity=27.509778357236, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI87081994, Length=752, Percent_Identity=23.8031914893617, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI145693196, Length=750, Percent_Identity=22.8, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1788534, Length=383, Percent_Identity=25.065274151436, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787231, Length=332, Percent_Identity=24.0963855421687, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1787741, Length=353, Percent_Identity=24.0793201133144, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 83984; Mature: 83853

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNIDITQAGVEVKKSACYFCHQNCGVLAYVKDGKVLKIEGDPEFPTNQGGLCCRGNIA
CCCCCCEECCCHHHHHHHHHEEECCCCEEEEECCCEEEEECCCCCCCCCCCCEEEECCEE
LQHLDHPARINYPLKRVGKRGEGKWEQIPWDQAMKEIGSKLLQIRSEFGAEAVATAGGTQ
EEECCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEECCCCCC
RTDDWSRRRFMNLFGSPNGFHNSHLCWIPTFMVETAIYGWCPFELDMGSSRCLILWGQNP
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCC
GAAGMPEMHHIRELQEKGMKLIVIDPRFSETAAKADLWLPLRPGSDLALALAWIHVIIFE
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHC
GLYDQEFVANCCEGFGELADHVENFTPEWAAELTWLTPEQIRAGAYMYAMNKPGNIQWGT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHHHCCEEEEEECCCCCEECCC
SVDQIGKPAGSTMHARAILRALTGNLDAPGSDLLTGPSPDFLTDEEMEANDQLPEEQKAK
CHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QIGSDRFKLVTWPGYSKIAEQTRKVWGKAPTAEWMCEAHPPSVFRSILSGKPYQVKALLV
HCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCHHHHHHHHCCCCEEEEEEEE
SATNPINSYGETSLVLDALKAVDFMVTCDYWMTPTAALSDYVIPIAGALERPTITSSYGC
ECCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCEECCCCCCCCCCCCCCCC
SDFLLASQRAIQPMYERRNDYNFWRDLGCAMGQQEMWPWATVEEAYYAKIAPLGYDIESY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCCHHHH
DEFVERYRFHFPEREYFKYQRQGFATPSGKVELYSTVLKDLGYPPLPPYIGPTENEIDDP
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
ELAKEFPLVLTTGGGFMPYHHSEHFQIKEVRFLRHEPYMDINPATAAELSIEDGDWVWIE
HHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEE
TKRGRIKQKANLTQGIHPRVIYTQRGWWYPERSMAAPELGGCLESNTNVLTTTADDHCDP
CCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCEEEECCCCCCCC
YSGSWANRGLLCRVYKVNASDMEGK
CCCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SKNIDITQAGVEVKKSACYFCHQNCGVLAYVKDGKVLKIEGDPEFPTNQGGLCCRGNIA
CCCCCEECCCHHHHHHHHHEEECCCCEEEEECCCEEEEECCCCCCCCCCCCEEEECCEE
LQHLDHPARINYPLKRVGKRGEGKWEQIPWDQAMKEIGSKLLQIRSEFGAEAVATAGGTQ
EEECCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEECCCCCC
RTDDWSRRRFMNLFGSPNGFHNSHLCWIPTFMVETAIYGWCPFELDMGSSRCLILWGQNP
CCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCC
GAAGMPEMHHIRELQEKGMKLIVIDPRFSETAAKADLWLPLRPGSDLALALAWIHVIIFE
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHCCCEEEEEECCCCHHHHHHHHHHHHHHC
GLYDQEFVANCCEGFGELADHVENFTPEWAAELTWLTPEQIRAGAYMYAMNKPGNIQWGT
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHHHCCEEEEEECCCCCEECCC
SVDQIGKPAGSTMHARAILRALTGNLDAPGSDLLTGPSPDFLTDEEMEANDQLPEEQKAK
CHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QIGSDRFKLVTWPGYSKIAEQTRKVWGKAPTAEWMCEAHPPSVFRSILSGKPYQVKALLV
HCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCHHHHHHHHCCCCEEEEEEEE
SATNPINSYGETSLVLDALKAVDFMVTCDYWMTPTAALSDYVIPIAGALERPTITSSYGC
ECCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCEECCCCCCCCCCCCCCCC
SDFLLASQRAIQPMYERRNDYNFWRDLGCAMGQQEMWPWATVEEAYYAKIAPLGYDIESY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHEECCCCCCHHHH
DEFVERYRFHFPEREYFKYQRQGFATPSGKVELYSTVLKDLGYPPLPPYIGPTENEIDDP
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
ELAKEFPLVLTTGGGFMPYHHSEHFQIKEVRFLRHEPYMDINPATAAELSIEDGDWVWIE
HHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEE
TKRGRIKQKANLTQGIHPRVIYTQRGWWYPERSMAAPELGGCLESNTNVLTTTADDHCDP
CCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCEEEECCCCCCCC
YSGSWANRGLLCRVYKVNASDMEGK
CCCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]