Click here to switch to the map view.

The map label for this gene is ycjG [C]

Identifier: 189423779

GI number: 189423779

Start: 725711

End: 726814

Strand: Reverse

Name: ycjG [C]

Synonym: Glov_0710

Alternate gene names: 189423779

Gene position: 726814-725711 (Counterclockwise)

Preceding gene: 189423780

Following gene: 189423778

Centisome position: 18.55

GC content: 59.78

Gene sequence:

>1104_bases
ATGGATCATATCATTATCAGGCACGCTCACGCCGCTGTCATCACCGCACCGCTGAACACGCCGTTCCGGATCGCCACCGG
CCAGCACGACCAGTTGGAAAACGTCTTTTTCCAGCTAAAGGCTCATGATGGAACCTGCGGTTACGGCGAGGCCGCGGTTG
CCAGTCATATTACCGGCGAAACCGTGGAGCAAACGCTGGCCAACCTGCAAAAAGCGGCAACGCAACTGGCCGGACGCAAC
GGCAACGATCCGGTTGAACTGATTGAGGAGTTTCGGCCCCTGTTTGTCGGCAACCATGCTGCCCTGGCCGCCTTTGAGAT
GGCGCTGTTCGATCTGGCCAGCCGCAGCCGGGGCATACCGTTTGGCGCCTTGTTCAGTGCTGCGCCGGGTTGTGCGGCCA
GACCACGCTTTCAAAGTGACATCACCATCGTAATCGGCAGCCTGGAGGAGGCGGAAACCGCTGCCCGCCACTATGCCGGA
CAGGGCTTCACCGCCTTCAAGATCAAGGTGGGCAAAGACCATGAGCTGGACCTGCAGCGGGTCTTGGCGGTCAAACGAAT
CGCACCCGACAGCTGCCTGGTGCTGGATGCCAACATGGGCTTTGATGCTACCGGCATGCGGCGTTTCCTGCAACAGCTGG
AACAGCACGGCATCCGCCCCGACCTGCTGGAACAACCGGTACCCAAGGGGGATTGGGACGGCCTGGCTGCCATTACGCGG
GAGTTTGCCGGTAGCGGCATGCTGATTTGTGCCGACGAAAGTGTCGGTTCCCTGGCAGCTGCAGAGCTGGCAGTCGCGCT
GAAAGCGGTCAATGCCATCAATATCAAATTTATGAAGAGCGGTATCACTGAGTCGGTCAGGATTGCCGAGCTGGCCTGCC
GCAACAACATCAAACTGATGCTGGGGGCCATGATGGAGAGCAGCCTGGCCATTACCGCCGCAGCCCATTTTGCCGCCACC
CTGGGCTGCTTTGAGGTGATTGACCTGGACACCACCTTCTTTATCAACGGCCCGCTCAGTCGCAGTCCCTATCTGGATCA
GAGCGGCAGGTTTGATCTGGGCGCCCCCGGACCCGGCATCGGCGTAGCGTTGAAGCTGTTGTAA

Upstream 100 bases:

>100_bases
TATTAAAACTGGTGGTATTCTAGACCAGCAACACAAGCAGTGGGTGATGGGTACCAGTATGTTGCCTGATACCTGTATAC
CCGCCTAAGGAACAATGAGC

Downstream 100 bases:

>100_bases
GACAACTCCCGCAGCAACAACTTTCTGGAAATAGATTGTACAAGTAGTACTATTGACATAACCTCTCTCAACGCATGACA
TTCTAACGCTAAAGGTGCCG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDHIIIRHAHAAVITAPLNTPFRIATGQHDQLENVFFQLKAHDGTCGYGEAAVASHITGETVEQTLANLQKAATQLAGRN
GNDPVELIEEFRPLFVGNHAALAAFEMALFDLASRSRGIPFGALFSAAPGCAARPRFQSDITIVIGSLEEAETAARHYAG
QGFTAFKIKVGKDHELDLQRVLAVKRIAPDSCLVLDANMGFDATGMRRFLQQLEQHGIRPDLLEQPVPKGDWDGLAAITR
EFAGSGMLICADESVGSLAAAELAVALKAVNAINIKFMKSGITESVRIAELACRNNIKLMLGAMMESSLAITAAAHFAAT
LGCFEVIDLDTTFFINGPLSRSPYLDQSGRFDLGAPGPGIGVALKLL

Sequences:

>Translated_367_residues
MDHIIIRHAHAAVITAPLNTPFRIATGQHDQLENVFFQLKAHDGTCGYGEAAVASHITGETVEQTLANLQKAATQLAGRN
GNDPVELIEEFRPLFVGNHAALAAFEMALFDLASRSRGIPFGALFSAAPGCAARPRFQSDITIVIGSLEEAETAARHYAG
QGFTAFKIKVGKDHELDLQRVLAVKRIAPDSCLVLDANMGFDATGMRRFLQQLEQHGIRPDLLEQPVPKGDWDGLAAITR
EFAGSGMLICADESVGSLAAAELAVALKAVNAINIKFMKSGITESVRIAELACRNNIKLMLGAMMESSLAITAAAHFAAT
LGCFEVIDLDTTFFINGPLSRSPYLDQSGRFDLGAPGPGIGVALKLL
>Mature_367_residues
MDHIIIRHAHAAVITAPLNTPFRIATGQHDQLENVFFQLKAHDGTCGYGEAAVASHITGETVEQTLANLQKAATQLAGRN
GNDPVELIEEFRPLFVGNHAALAAFEMALFDLASRSRGIPFGALFSAAPGCAARPRFQSDITIVIGSLEEAETAARHYAG
QGFTAFKIKVGKDHELDLQRVLAVKRIAPDSCLVLDANMGFDATGMRRFLQQLEQHGIRPDLLEQPVPKGDWDGLAAITR
EFAGSGMLICADESVGSLAAAELAVALKAVNAINIKFMKSGITESVRIAELACRNNIKLMLGAMMESSLAITAAAHFAAT
LGCFEVIDLDTTFFINGPLSRSPYLDQSGRFDLGAPGPGIGVALKLL

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=300, Percent_Identity=29.6666666666667, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39178; Mature: 39178

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIIIRHAHAAVITAPLNTPFRIATGQHDQLENVFFQLKAHDGTCGYGEAAVASHITGE
CCCEEEEECCEEEEEECCCCCEEEECCCCHHHHEEEEEEEECCCCCCCCCHHHHHHCCHH
TVEQTLANLQKAATQLAGRNGNDPVELIEEFRPLFVGNHAALAAFEMALFDLASRSRGIP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHEECCHHHHHHHHHHHHHHHHHCCCCC
FGALFSAAPGCAARPRFQSDITIVIGSLEEAETAARHYAGQGFTAFKIKVGKDHELDLQR
HHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
VLAVKRIAPDSCLVLDANMGFDATGMRRFLQQLEQHGIRPDLLEQPVPKGDWDGLAAITR
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHH
EFAGSGMLICADESVGSLAAAELAVALKAVNAINIKFMKSGITESVRIAELACRNNIKLM
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCHHHH
LGAMMESSLAITAAAHFAATLGCFEVIDLDTTFFINGPLSRSPYLDQSGRFDLGAPGPGI
HHHHHHCCHHHHHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GVALKLL
CEEEEEC
>Mature Secondary Structure
MDHIIIRHAHAAVITAPLNTPFRIATGQHDQLENVFFQLKAHDGTCGYGEAAVASHITGE
CCCEEEEECCEEEEEECCCCCEEEECCCCHHHHEEEEEEEECCCCCCCCCHHHHHHCCHH
TVEQTLANLQKAATQLAGRNGNDPVELIEEFRPLFVGNHAALAAFEMALFDLASRSRGIP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHEECCHHHHHHHHHHHHHHHHHCCCCC
FGALFSAAPGCAARPRFQSDITIVIGSLEEAETAARHYAGQGFTAFKIKVGKDHELDLQR
HHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHH
VLAVKRIAPDSCLVLDANMGFDATGMRRFLQQLEQHGIRPDLLEQPVPKGDWDGLAAITR
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHH
EFAGSGMLICADESVGSLAAAELAVALKAVNAINIKFMKSGITESVRIAELACRNNIKLM
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCHHHH
LGAMMESSLAITAAAHFAATLGCFEVIDLDTTFFINGPLSRSPYLDQSGRFDLGAPGPGI
HHHHHHCCHHHHHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GVALKLL
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]