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The map label for this gene is ddl

Identifier: 189423736

GI number: 189423736

Start: 673788

End: 674729

Strand: Reverse

Name: ddl

Synonym: Glov_0667

Alternate gene names: 189423736

Gene position: 674729-673788 (Counterclockwise)

Preceding gene: 189423737

Following gene: 189423735

Centisome position: 17.22

GC content: 57.75

Gene sequence:

>942_bases
ATGACGCGTGACGAACTGAAACAGAAACGGATCGGTGTGTTGATGGGGGGGCTGTCGGCTGAACGGGATGTCTCGCTCAA
AAGCGGCATGGCAGTCCATCAGGCCTTGCTGGCAATGGGCTATGACAGCACGGCCCTCGATGTGCGCCACAACGTGGCCC
TCATACTGCGTGAAGAAAAGATCGATCTGGCCTTCATTGCACTGCACGGCCGCTACGGCGAGGATGGCTGTATTCAGGGG
CTGCTGGAACTGATGCAGATCCCCTATACCGGCTCCGGTGTGCTGGCCAGTGCCCTTGCCATGCACAAGCTCTACAGCAA
GCAGGCCTTTGCCGCTGCCGGTCTGACCATCACCCCCTATGCGACAGTCAGACAGGGAGACCGTTGTAACGCAGAACAAC
TGCCGTTCGGCCTGCCGGTGGTGGTCAAGCCGGTTCAGGAAGGCTCGTCAGTCGGTGTCACCATCGTGAAAAAACCGGAG
GATCTTCAGGCCGCCCTGGATGAGGCCTTCCGCTATGACACACTGGTACTGGTGGAAAAGTACATCAAAGGCCAGGAGGT
CCAGGTCGGCATTCTGGCGAATCAACCGATCGGCGCCATCGAGATCGTACCAAAGAACGAGTTCTACGATTTTGAGGCCA
AATACAGTGACGGCATGGCTGAGCATATTTTCCCTGCCCGCCTTGCCGAGCCGCTCTACCAAAAAGTACAACAGCAAGGA
TTGCAGGCACATCAGGCGTTGGGCTGTGACGGTTACTGCCGGGTGGACTTTCTGGTTACTGAAAACGGTGACTGCTACCT
GTTGGAGGTCAACACCCTGCCCGGCATGACCGCCCTGTCGCTTCTGCCGGAGATTGCACAAAAAGGTGCCGGTCTTTCAT
TTGAAGCGCTGGTGGAGCAGATCGCCTGCTCGGCAGCACTCAAGACGGGTCAGGCGGGGTGA

Upstream 100 bases:

>100_bases
TTCGGGCACTGGCGGCAGCAGTCAAGGAACGGGTTAAGGCAACGTGTGGTATCGAGCTTGAGGAAGAAGTGCAACTGCTT
GACAGCGGGGAGTGCGGGCA

Downstream 100 bases:

>100_bases
TGACCGATCTGAGGCATATCGGCAGCCACAAAGGGAAAAAAGTGGCGTCGAATAAGCTGAAGGTCAAGAAGGAACCGGTT
GACCTGCGCAAATACCTGTT

Product: D-alanine/D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTRDELKQKRIGVLMGGLSAERDVSLKSGMAVHQALLAMGYDSTALDVRHNVALILREEKIDLAFIALHGRYGEDGCIQG
LLELMQIPYTGSGVLASALAMHKLYSKQAFAAAGLTITPYATVRQGDRCNAEQLPFGLPVVVKPVQEGSSVGVTIVKKPE
DLQAALDEAFRYDTLVLVEKYIKGQEVQVGILANQPIGAIEIVPKNEFYDFEAKYSDGMAEHIFPARLAEPLYQKVQQQG
LQAHQALGCDGYCRVDFLVTENGDCYLLEVNTLPGMTALSLLPEIAQKGAGLSFEALVEQIACSAALKTGQAG

Sequences:

>Translated_313_residues
MTRDELKQKRIGVLMGGLSAERDVSLKSGMAVHQALLAMGYDSTALDVRHNVALILREEKIDLAFIALHGRYGEDGCIQG
LLELMQIPYTGSGVLASALAMHKLYSKQAFAAAGLTITPYATVRQGDRCNAEQLPFGLPVVVKPVQEGSSVGVTIVKKPE
DLQAALDEAFRYDTLVLVEKYIKGQEVQVGILANQPIGAIEIVPKNEFYDFEAKYSDGMAEHIFPARLAEPLYQKVQQQG
LQAHQALGCDGYCRVDFLVTENGDCYLLEVNTLPGMTALSLLPEIAQKGAGLSFEALVEQIACSAALKTGQAG
>Mature_312_residues
TRDELKQKRIGVLMGGLSAERDVSLKSGMAVHQALLAMGYDSTALDVRHNVALILREEKIDLAFIALHGRYGEDGCIQGL
LELMQIPYTGSGVLASALAMHKLYSKQAFAAAGLTITPYATVRQGDRCNAEQLPFGLPVVVKPVQEGSSVGVTIVKKPED
LQAALDEAFRYDTLVLVEKYIKGQEVQVGILANQPIGAIEIVPKNEFYDFEAKYSDGMAEHIFPARLAEPLYQKVQQQGL
QAHQALGCDGYCRVDFLVTENGDCYLLEVNTLPGMTALSLLPEIAQKGAGLSFEALVEQIACSAALKTGQAG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=299, Percent_Identity=45.1505016722408, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1786579, Length=363, Percent_Identity=32.7823691460055, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_GEOLS (B3E3X9)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001950913.1
- ProteinModelPortal:   B3E3X9
- SMR:   B3E3X9
- GeneID:   6369060
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0667
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   CLSK829085
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33830; Mature: 33699

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDELKQKRIGVLMGGLSAERDVSLKSGMAVHQALLAMGYDSTALDVRHNVALILREEK
CCHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCEEECCCCEEEEEEECC
IDLAFIALHGRYGEDGCIQGLLELMQIPYTGSGVLASALAMHKLYSKQAFAAAGLTITPY
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCC
ATVRQGDRCNAEQLPFGLPVVVKPVQEGSSVGVTIVKKPEDLQAALDEAFRYDTLVLVEK
HHHCCCCCCCHHHCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
YIKGQEVQVGILANQPIGAIEIVPKNEFYDFEAKYSDGMAEHIFPARLAEPLYQKVQQQG
HHCCCEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHH
LQAHQALGCDGYCRVDFLVTENGDCYLLEVNTLPGMTALSLLPEIAQKGAGLSFEALVEQ
HHHHHHCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
IACSAALKTGQAG
HHHHHHHHCCCCC
>Mature Secondary Structure 
TRDELKQKRIGVLMGGLSAERDVSLKSGMAVHQALLAMGYDSTALDVRHNVALILREEK
CHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHCCCCCEEECCCCEEEEEEECC
IDLAFIALHGRYGEDGCIQGLLELMQIPYTGSGVLASALAMHKLYSKQAFAAAGLTITPY
CCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCC
ATVRQGDRCNAEQLPFGLPVVVKPVQEGSSVGVTIVKKPEDLQAALDEAFRYDTLVLVEK
HHHCCCCCCCHHHCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
YIKGQEVQVGILANQPIGAIEIVPKNEFYDFEAKYSDGMAEHIFPARLAEPLYQKVQQQG
HHCCCEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHH
LQAHQALGCDGYCRVDFLVTENGDCYLLEVNTLPGMTALSLLPEIAQKGAGLSFEALVEQ
HHHHHHCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
IACSAALKTGQAG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA