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The map label for this gene is purT [H]

Identifier: 189423505

GI number: 189423505

Start: 408660

End: 409844

Strand: Reverse

Name: purT [H]

Synonym: Glov_0434

Alternate gene names: 189423505

Gene position: 409844-408660 (Counterclockwise)

Preceding gene: 189423506

Following gene: 189423504

Centisome position: 10.46

GC content: 64.81

Gene sequence:

>1185_bases
ATGAGCATTGGAACCCCGCTGAAGGCCGGTGCCACCAAACTGCTGTTGTTGGGATCAGGAGAACTGGGCAAGGAAGTTGC
CCTGGAGGCGCAACGCCTGGGAGTGGAGGTGATCGCGGTTGACCGCTATGCCGATGCCCCGGCCATGCAGGTGGCCCACC
GCAGCCATGTGATCAGCATGCTGGACCGGGAGGCGCTGCGCCGGGTGATTGAGCAGGAGCGGCCCGACCTGATCGTGCCG
GAGATCGAGGCGATTGATACGGTCTTTCTGCTGGAACTGGAGCAGGCAGGCCAGCGGGTGATCCCTACGGCCCGGGCTGC
CAACCTGACCATGAACCGCGAGGGTATCCGGCGTCTGGCAGCTGAGGAGCTGGGGCTGCCCACCGCCCCCTATGCCTTTG
CCTCCAGTGCGGCAGAGCTGCGGGCTGCTGCCGACAGCATCGGGTTCCCCTGCGTGGTCAAGCCGATCATGAGTTCATCC
GGCAAGGGGCAGAGCGTGGTGAAAACCGCAGCCGAGGTGGATACGGCCTGGCAGTACGCCATGGATGGCGCCCGCGGTGC
CTCGGATACGGTCATCATTGAAGGGTTTATCGATTTTGACTACGAGATCACCCAGCTGACCGTGCGCCATGCCGGCGGCA
CCTCCTTCTGCCCGCCGATCGGCCATGTGCAGATCAAGGGGGATTACCACGAATCATGGCAGCCGATGGCCATGTCACCG
GCAGCCCTGGCTGAGGCGCGGCGTCAGGCGGAGATGGTCACCACCGCCCTGGGCGGTTACGGGATCTTCGGGGTGGAGCT
GTTTATCAAGGGGGATACGGTGCTGTTCAGCGAGGTCTCACCCCGCCCCCATGACACCGGCATGGTGACCATGATCTCCC
AGAACCTGAGCCAGTTTGAGCTGCATGTGCGGGCCATCCTGGGGCTGCCGGTGCCTGAGATCCTGAATCTGGCCCCCTCT
GCCAGCCATGTCATCCTGGCAACTGACAGCCAGGAGACGGTGCGCTTTACAGGTGTTGCAGAGGCCTTGAGCGTTCCCAC
GGCCAAGCTGCGCCTGTTCGGCAAGCCGGATACCCGCCCGGGCCGCCGCATGGGGGTTGCCCTGACCCAGGGGGCCAGCA
CCGATGAGGCCCGCAGCCGGGCCGAGGCCAGCGCCCATTGTGTACGGATTGTGCCGCAGGGATAG

Upstream 100 bases:

>100_bases
GCGTTGACCTGAACTGGCCGGTCCAGTAAAAATCCGTTCCCATCGCCAGACTATGTAATGCATACTGCAAAGAATTTTTA
CCCATGGAGGATGTTACCGT

Downstream 100 bases:

>100_bases
CCTGCTTGTAATGTAATCTGTATCCGCAGCAAAAAATAGAAAAGGAAACCAGCAATGAGTGAATTACCGAAATGCCCGTC
CTGCAGTTCCAGCTACACCT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSIGTPLKAGATKLLLLGSGELGKEVALEAQRLGVEVIAVDRYADAPAMQVAHRSHVISMLDREALRRVIEQERPDLIVP
EIEAIDTVFLLELEQAGQRVIPTARAANLTMNREGIRRLAAEELGLPTAPYAFASSAAELRAAADSIGFPCVVKPIMSSS
GKGQSVVKTAAEVDTAWQYAMDGARGASDTVIIEGFIDFDYEITQLTVRHAGGTSFCPPIGHVQIKGDYHESWQPMAMSP
AALAEARRQAEMVTTALGGYGIFGVELFIKGDTVLFSEVSPRPHDTGMVTMISQNLSQFELHVRAILGLPVPEILNLAPS
ASHVILATDSQETVRFTGVAEALSVPTAKLRLFGKPDTRPGRRMGVALTQGASTDEARSRAEASAHCVRIVPQG

Sequences:

>Translated_394_residues
MSIGTPLKAGATKLLLLGSGELGKEVALEAQRLGVEVIAVDRYADAPAMQVAHRSHVISMLDREALRRVIEQERPDLIVP
EIEAIDTVFLLELEQAGQRVIPTARAANLTMNREGIRRLAAEELGLPTAPYAFASSAAELRAAADSIGFPCVVKPIMSSS
GKGQSVVKTAAEVDTAWQYAMDGARGASDTVIIEGFIDFDYEITQLTVRHAGGTSFCPPIGHVQIKGDYHESWQPMAMSP
AALAEARRQAEMVTTALGGYGIFGVELFIKGDTVLFSEVSPRPHDTGMVTMISQNLSQFELHVRAILGLPVPEILNLAPS
ASHVILATDSQETVRFTGVAEALSVPTAKLRLFGKPDTRPGRRMGVALTQGASTDEARSRAEASAHCVRIVPQG
>Mature_393_residues
SIGTPLKAGATKLLLLGSGELGKEVALEAQRLGVEVIAVDRYADAPAMQVAHRSHVISMLDREALRRVIEQERPDLIVPE
IEAIDTVFLLELEQAGQRVIPTARAANLTMNREGIRRLAAEELGLPTAPYAFASSAAELRAAADSIGFPCVVKPIMSSSG
KGQSVVKTAAEVDTAWQYAMDGARGASDTVIIEGFIDFDYEITQLTVRHAGGTSFCPPIGHVQIKGDYHESWQPMAMSPA
ALAEARRQAEMVTTALGGYGIFGVELFIKGDTVLFSEVSPRPHDTGMVTMISQNLSQFELHVRAILGLPVPEILNLAPSA
SHVILATDSQETVRFTGVAEALSVPTAKLRLFGKPDTRPGRRMGVALTQGASTDEARSRAEASAHCVRIVPQG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=388, Percent_Identity=59.5360824742268, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786733, Length=246, Percent_Identity=27.2357723577236, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=383, Percent_Identity=27.154046997389, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42158; Mature: 42027

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGTPLKAGATKLLLLGSGELGKEVALEAQRLGVEVIAVDRYADAPAMQVAHRSHVISM
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHH
LDREALRRVIEQERPDLIVPEIEAIDTVFLLELEQAGQRVIPTARAANLTMNREGIRRLA
HHHHHHHHHHHHCCCCEECCCHHHHCEEEEEEHHHCCCEECCCHHHCCEEECHHHHHHHH
AEELGLPTAPYAFASSAAELRAAADSIGFPCVVKPIMSSSGKGQSVVKTAAEVDTAWQYA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHH
MDGARGASDTVIIEGFIDFDYEITQLTVRHAGGTSFCPPIGHVQIKGDYHESWQPMAMSP
HCCCCCCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCH
AALAEARRQAEMVTTALGGYGIFGVELFIKGDTVLFSEVSPRPHDTGMVTMISQNLSQFE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHH
LHVRAILGLPVPEILNLAPSASHVILATDSQETVRFTGVAEALSVPTAKLRLFGKPDTRP
HHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCEEEEEEHHHHHHCCCEEEEEECCCCCCC
GRRMGVALTQGASTDEARSRAEASAHCVRIVPQG
CCEEEEEEECCCCCHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SIGTPLKAGATKLLLLGSGELGKEVALEAQRLGVEVIAVDRYADAPAMQVAHRSHVISM
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHH
LDREALRRVIEQERPDLIVPEIEAIDTVFLLELEQAGQRVIPTARAANLTMNREGIRRLA
HHHHHHHHHHHHCCCCEECCCHHHHCEEEEEEHHHCCCEECCCHHHCCEEECHHHHHHHH
AEELGLPTAPYAFASSAAELRAAADSIGFPCVVKPIMSSSGKGQSVVKTAAEVDTAWQYA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHH
MDGARGASDTVIIEGFIDFDYEITQLTVRHAGGTSFCPPIGHVQIKGDYHESWQPMAMSP
HCCCCCCCCEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCH
AALAEARRQAEMVTTALGGYGIFGVELFIKGDTVLFSEVSPRPHDTGMVTMISQNLSQFE
HHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHH
LHVRAILGLPVPEILNLAPSASHVILATDSQETVRFTGVAEALSVPTAKLRLFGKPDTRP
HHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCEEEEEEHHHHHHCCCEEEEEECCCCCCC
GRRMGVALTQGASTDEARSRAEASAHCVRIVPQG
CCEEEEEEECCCCCHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA