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The map label for this gene is vacJ [H]

Identifier: 189354089

GI number: 189354089

Start: 2434793

End: 2435806

Strand: Direct

Name: vacJ [H]

Synonym: BMULJ_05342

Alternate gene names: 189354089

Gene position: 2434793-2435806 (Clockwise)

Preceding gene: 189354088

Following gene: 189354099

Centisome position: 98.45

GC content: 69.63

Gene sequence:

>1014_bases
ATGAATAACATGCGAATCATCGCGGTGACCGTCGCCGCAAGCGCGATGCTGTCCGGCTGCGCGACGGGACCCAACCGCAA
CCCCAACGACCCGCTCGAGCCGATGAACCGCGCGATGTACAAGTTCAACGACACGGTCGACGAGCACATCGCGGTGCCGA
TCGCGAAGGGCTACCAGAAGATCACGCCGACGCCGCTGCGTACCGCGATCAGCAACTTCTTCTCGAACCTCGGCGATCTC
GGCAACATGGCGAACAACCTGCTGCAGCTGCGGATCACCGACGCGACGCAGGATCTGATGCGCGTCGCGATGAACTCGGT
GTTCGGCATGGCCGGACTGATCGACATCGCGACACCCGCGGGGCTGCCGAAGCATCACCAGGACTTCGGGCTGACGCTCG
CGCGCTGGGGCGTGCCGTCGGGCCCGTACCTCGTGCTGCCGGTGTTCGGGCCGAGCACGTTCCGCGACGGCGTCGGCCGC
GCGGTCGACGTGCGCTTCAACCTGCTCAACTACATCGAGCCGGCTGCGCGCAATCCGATGTACGTGATGCAGTTCATCAG
CGCGCGTTCCGATCTGCTCGGCGCGACCGATCTGCTGAAGCAGGCCGCGCTCGATCCGTATTCGTTCGTGCGCGACGCGT
ATCTGCAGCAGCGCAAGTCGCTCACGTACCGCGGCCAGTCGTCGGCCGGGGTGCCGAGCTACAACGAGCCGGGCGAAGCG
GGCGCCGTGCCGGGCGCGGTGCCGGCAGCGACGCCGGCCGCGCCGGGGTTGCCGAATTACGAGGATCCGGGCGAATCGGG
CGGCACGGGCGGCGGCGCGCCGAACAACGCGCCGGCCGCGCCGGGATTGCCGCAGTACGACGATCCGGGTTCGGACACCG
CGACGCCGGCCAGCGCCCCGGCTGCCGCGTCGGCCACCGCACCGGCCGGCTCGACGACAGCACCGGCCGCGAGTGGTGCG
CCGATCGCGCCGGCGATGCCGACCGTGCCCGCGAGCAGTCCGGCCGCGCAGTAA

Upstream 100 bases:

>100_bases
CTGGCGCTGACCTGTACGCTGATCGGCGCCGTCGTGTTTCAGCCCGTCCTGATGGGCAAACCGCGCGTGAAGCGCGCGAA
GAACCAATCGCAAGGAATCA

Downstream 100 bases:

>100_bases
GGTCGGCGGGCTCGACGCTCGCTACTCTGTCAAAGCCCGCTGTCAGGCATTGCCGGCAATCTGCCGTGCGGCAATCCCGG
CGGCGGGCCGCTATCCGGCA

Product: lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNNMRIIAVTVAASAMLSGCATGPNRNPNDPLEPMNRAMYKFNDTVDEHIAVPIAKGYQKITPTPLRTAISNFFSNLGDL
GNMANNLLQLRITDATQDLMRVAMNSVFGMAGLIDIATPAGLPKHHQDFGLTLARWGVPSGPYLVLPVFGPSTFRDGVGR
AVDVRFNLLNYIEPAARNPMYVMQFISARSDLLGATDLLKQAALDPYSFVRDAYLQQRKSLTYRGQSSAGVPSYNEPGEA
GAVPGAVPAATPAAPGLPNYEDPGESGGTGGGAPNNAPAAPGLPQYDDPGSDTATPASAPAAASATAPAGSTTAPAASGA
PIAPAMPTVPASSPAAQ

Sequences:

>Translated_337_residues
MNNMRIIAVTVAASAMLSGCATGPNRNPNDPLEPMNRAMYKFNDTVDEHIAVPIAKGYQKITPTPLRTAISNFFSNLGDL
GNMANNLLQLRITDATQDLMRVAMNSVFGMAGLIDIATPAGLPKHHQDFGLTLARWGVPSGPYLVLPVFGPSTFRDGVGR
AVDVRFNLLNYIEPAARNPMYVMQFISARSDLLGATDLLKQAALDPYSFVRDAYLQQRKSLTYRGQSSAGVPSYNEPGEA
GAVPGAVPAATPAAPGLPNYEDPGESGGTGGGAPNNAPAAPGLPQYDDPGSDTATPASAPAAASATAPAGSTTAPAASGA
PIAPAMPTVPASSPAAQ
>Mature_337_residues
MNNMRIIAVTVAASAMLSGCATGPNRNPNDPLEPMNRAMYKFNDTVDEHIAVPIAKGYQKITPTPLRTAISNFFSNLGDL
GNMANNLLQLRITDATQDLMRVAMNSVFGMAGLIDIATPAGLPKHHQDFGLTLARWGVPSGPYLVLPVFGPSTFRDGVGR
AVDVRFNLLNYIEPAARNPMYVMQFISARSDLLGATDLLKQAALDPYSFVRDAYLQQRKSLTYRGQSSAGVPSYNEPGEA
GAVPGAVPAATPAAPGLPNYEDPGESGGTGGGAPNNAPAAPGLPQYDDPGSDTATPASAPAAASATAPAGSTTAPAASGA
PIAPAMPTVPASSPAAQ

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=231, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 34760; Mature: 34760

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMRIIAVTVAASAMLSGCATGPNRNPNDPLEPMNRAMYKFNDTVDEHIAVPIAKGYQK
CCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHCCHH
ITPTPLRTAISNFFSNLGDLGNMANNLLQLRITDATQDLMRVAMNSVFGMAGLIDIATPA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLPKHHQDFGLTLARWGVPSGPYLVLPVFGPSTFRDGVGRAVDVRFNLLNYIEPAARNPM
CCCCCCHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHCCCEEEHHHHHHHHHCHHHCCHH
YVMQFISARSDLLGATDLLKQAALDPYSFVRDAYLQQRKSLTYRGQSSAGVPSYNEPGEA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GAVPGAVPAATPAAPGLPNYEDPGESGGTGGGAPNNAPAAPGLPQYDDPGSDTATPASAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAASATAPAGSTTAPAASGAPIAPAMPTVPASSPAAQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNMRIIAVTVAASAMLSGCATGPNRNPNDPLEPMNRAMYKFNDTVDEHIAVPIAKGYQK
CCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHCCHH
ITPTPLRTAISNFFSNLGDLGNMANNLLQLRITDATQDLMRVAMNSVFGMAGLIDIATPA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLPKHHQDFGLTLARWGVPSGPYLVLPVFGPSTFRDGVGRAVDVRFNLLNYIEPAARNPM
CCCCCCHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHCCCEEEHHHHHHHHHCHHHCCHH
YVMQFISARSDLLGATDLLKQAALDPYSFVRDAYLQQRKSLTYRGQSSAGVPSYNEPGEA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GAVPGAVPAATPAAPGLPNYEDPGESGGTGGGAPNNAPAAPGLPQYDDPGSDTATPASAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAASATAPAGSTTAPAASGAPIAPAMPTVPASSPAAQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]