Click here to switch to the map view.

The map label for this gene is lacZ

Identifier: 189354075

GI number: 189354075

Start: 2411363

End: 2413348

Strand: Direct

Name: lacZ

Synonym: BMULJ_05328

Alternate gene names: NA

Gene position: 2411363-2413348 (Clockwise)

Preceding gene: 189354074

Following gene: 189354076

Centisome position: 97.5

GC content: 70.29

Gene sequence:

>1986_bases
ATGCGCGTCGGCGTCTGTTACTACCCGGAACACTGGCCGGAATCGATGTGGGCGGACGACGCCCGCCGGATGAAAGCGCT
CGGCATCGCTCAGGTGCGCATCGCGGAATTCGCGTGGAGCCGCATCGAGCCGTCGCCCGGCGAATACGATTGGGGCTGGC
TCGATCGCGCGGTCGACGTGCTCGCCGCGGCCGGCCTCGACATCGTGATGTGCACGCCGACCGCGACGCCGCCGAAATGG
CTGATCGACCGCCATCCCGAGATCCTCGCGATCGGCGCGGACGGCCGGCCGCGCACGTTCGGCTCGCGCCGCCACTACGA
CTTCTCGTCGCCGGTCTATTACGACGCGTCGCGGCAGATCTGCACGGCGGTCGCCGAGCGCTACGGCCGCCATCCGGCCG
TGCGCTTCTGGCAGACCGACAACGAGATGGGCTGCCACCAGACGGTCGTCAGCTATTCGCCGGCCGCGCTCGCACGCTTT
CGCGAATGGCTGAAGGCGCGCTACGGGACGATCGACGCACTGAACCGCGCGTGGGGCACCGTGTTCTGGAGCATGGAGTA
TCGCGATTTCGACGAGATCGACGCGCCCGTCGGCACCGTGACCGAAGCGCATCCGACGCATCGGCTCGACTACCGGCGTT
TCGCGTCGGACGAAGTGGTCCGCTATCACCGGATGCAGGTCGACGTGATCCGCGCGCACTCGCCGGGCCGGCCGGTCGCG
CACAACTTCATGCAGCTGTTCACCGACTTCGATCACTATGCGGTCGCGCGCGATCTCGACGTCGCGACGTGGGACAGCTA
TCCGCTCGGCGCGCTGGAGGAACACTGGTTCGCGCCCGACGTGAAGGCGCGCTGGCTGCGCACGGGCCATCCGGATTTCG
CGTCGTTCAATCACGACCTCTATCGCGGGATGTCGAAGCTGCCGTTCTGGGTGATGGAGCAGCAGCCGGGCCCCGTGAAC
TGGGCGCACTGGAACCCGGCGCCGCTGCCGGGCATGGTGCGGCTGTGGAGCTGGGAAGCCTTCGCGCACGGCGCGGGCTG
CGTGTCGTACTTCCGCTGGCGGCAGGCGCCGTTCGCGCAGGAACAGATGCATGCGGGCCTGCACACGCCCGACGACCGGC
TCGACGAAGGCGGCCGCGAAGCAGAGCGCGTCGCGCACGAGATCGCCGTCGTGCACGGCGCCGATGCACACGCGGACGCC
GCCGTGCATGCGCGCGTCGCGCTCGTCTTCGACTACGAAGCGAAGTGGCTGCTCGACGTGCAGCCGCAAGGCGCGGACTT
CCAGTACGCACGCATCGCGTTCGAGTACTACTCGGCGCTGCGCGCGCTCGGCCTCGACGTCGACGTGATCTCCGCGCAGG
CGCCGCTCGACGGCTATCGGCTCGTCGTCGTGCCGCCGCTGCCGATCGTTCCCGACGATTTCGCCGCGCGCCTCGCCGCA
TCGGGCGCGCATGCCGTGTTCGGGCCGCGCACCGGTTCGAAGACGGTCGACCTGCAGATTCCGCCGACGCTGCCGCCCGG
CGCACTCGCCTCGATCCTGCCGCTGCGCGTGTGGCGCGTCGAATCGCTGCGGCCGAACGTGACCGAACGCGTCGACGGCG
CGCTCGTCGACGGCACGCCGTTCGCCGGTCACGCGCGCCACTGGCGCGACCTCGTCGAGCTGCGCGATGCGCAGCGCACC
GTCGTGCGCGCGCGCTTTGCCGACGATCATCCGGCATGGCTGTCGCACGGCACGCTGCATTACTGGGCCGCGCTGTTCGA
CGACGCGACCACCGCGCAGCTGTTCGCGAGCGTCGCCGCCGAAGCGGGGCTCGCGCCGACGCCGCTCGCCGACGGCGTGC
GCATCAGCCGGCGCGGCGGGCTCACGTACGTCTTCAACTACGGCGACACCGTGCACACGATCGATGCCGTGCCGCCGGCC
GCGTTCGTGGTCGGCAGCGCGGACGTCGAGCCGCGCGGCGTCGCCGTCTATCGCAGCCCGGTGTAG

Upstream 100 bases:

>100_bases
GCATGCACCGGCCGCCGCGTGCCACCTGTTTACCGAAGACGGCCGCGCGCTGCCTGCGTCCGCCACCCTCTCCGTTCACC
ATTACGCATAAGGATCGCGC

Downstream 100 bases:

>100_bases
CGCTTTTCATCGTCACCGCCGCGGCGCGCGATGCGCCGCCCACGCATAACGACAGACAGGACTGGAGACAACCATGACTC
ATCGCCCCGTTCGCGCCGCC

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MRVGVCYYPEHWPESMWADDARRMKALGIAQVRIAEFAWSRIEPSPGEYDWGWLDRAVDVLAAAGLDIVMCTPTATPPKW
LIDRHPEILAIGADGRPRTFGSRRHYDFSSPVYYDASRQICTAVAERYGRHPAVRFWQTDNEMGCHQTVVSYSPAALARF
REWLKARYGTIDALNRAWGTVFWSMEYRDFDEIDAPVGTVTEAHPTHRLDYRRFASDEVVRYHRMQVDVIRAHSPGRPVA
HNFMQLFTDFDHYAVARDLDVATWDSYPLGALEEHWFAPDVKARWLRTGHPDFASFNHDLYRGMSKLPFWVMEQQPGPVN
WAHWNPAPLPGMVRLWSWEAFAHGAGCVSYFRWRQAPFAQEQMHAGLHTPDDRLDEGGREAERVAHEIAVVHGADAHADA
AVHARVALVFDYEAKWLLDVQPQGADFQYARIAFEYYSALRALGLDVDVISAQAPLDGYRLVVVPPLPIVPDDFAARLAA
SGAHAVFGPRTGSKTVDLQIPPTLPPGALASILPLRVWRVESLRPNVTERVDGALVDGTPFAGHARHWRDLVELRDAQRT
VVRARFADDHPAWLSHGTLHYWAALFDDATTAQLFASVAAEAGLAPTPLADGVRISRRGGLTYVFNYGDTVHTIDAVPPA
AFVVGSADVEPRGVAVYRSPV

Sequences:

>Translated_661_residues
MRVGVCYYPEHWPESMWADDARRMKALGIAQVRIAEFAWSRIEPSPGEYDWGWLDRAVDVLAAAGLDIVMCTPTATPPKW
LIDRHPEILAIGADGRPRTFGSRRHYDFSSPVYYDASRQICTAVAERYGRHPAVRFWQTDNEMGCHQTVVSYSPAALARF
REWLKARYGTIDALNRAWGTVFWSMEYRDFDEIDAPVGTVTEAHPTHRLDYRRFASDEVVRYHRMQVDVIRAHSPGRPVA
HNFMQLFTDFDHYAVARDLDVATWDSYPLGALEEHWFAPDVKARWLRTGHPDFASFNHDLYRGMSKLPFWVMEQQPGPVN
WAHWNPAPLPGMVRLWSWEAFAHGAGCVSYFRWRQAPFAQEQMHAGLHTPDDRLDEGGREAERVAHEIAVVHGADAHADA
AVHARVALVFDYEAKWLLDVQPQGADFQYARIAFEYYSALRALGLDVDVISAQAPLDGYRLVVVPPLPIVPDDFAARLAA
SGAHAVFGPRTGSKTVDLQIPPTLPPGALASILPLRVWRVESLRPNVTERVDGALVDGTPFAGHARHWRDLVELRDAQRT
VVRARFADDHPAWLSHGTLHYWAALFDDATTAQLFASVAAEAGLAPTPLADGVRISRRGGLTYVFNYGDTVHTIDAVPPA
AFVVGSADVEPRGVAVYRSPV
>Mature_661_residues
MRVGVCYYPEHWPESMWADDARRMKALGIAQVRIAEFAWSRIEPSPGEYDWGWLDRAVDVLAAAGLDIVMCTPTATPPKW
LIDRHPEILAIGADGRPRTFGSRRHYDFSSPVYYDASRQICTAVAERYGRHPAVRFWQTDNEMGCHQTVVSYSPAALARF
REWLKARYGTIDALNRAWGTVFWSMEYRDFDEIDAPVGTVTEAHPTHRLDYRRFASDEVVRYHRMQVDVIRAHSPGRPVA
HNFMQLFTDFDHYAVARDLDVATWDSYPLGALEEHWFAPDVKARWLRTGHPDFASFNHDLYRGMSKLPFWVMEQQPGPVN
WAHWNPAPLPGMVRLWSWEAFAHGAGCVSYFRWRQAPFAQEQMHAGLHTPDDRLDEGGREAERVAHEIAVVHGADAHADA
AVHARVALVFDYEAKWLLDVQPQGADFQYARIAFEYYSALRALGLDVDVISAQAPLDGYRLVVVPPLPIVPDDFAARLAA
SGAHAVFGPRTGSKTVDLQIPPTLPPGALASILPLRVWRVESLRPNVTERVDGALVDGTPFAGHARHWRDLVELRDAQRT
VVRARFADDHPAWLSHGTLHYWAALFDDATTAQLFASVAAEAGLAPTPLADGVRISRRGGLTYVFNYGDTVHTIDAVPPA
AFVVGSADVEPRGVAVYRSPV

Specific function: Unknown

COG id: COG1874

COG function: function code G; Beta-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 42 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013739
- InterPro:   IPR013738
- InterPro:   IPR013780
- InterPro:   IPR003476
- InterPro:   IPR013529
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02449 Glyco_hydro_42; PF08533 Glyco_hydro_42C; PF08532 Glyco_hydro_42M [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 74176; Mature: 74176

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGVCYYPEHWPESMWADDARRMKALGIAQVRIAEFAWSRIEPSPGEYDWGWLDRAVDV
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LAAAGLDIVMCTPTATPPKWLIDRHPEILAIGADGRPRTFGSRRHYDFSSPVYYDASRQI
HHHCCCEEEEECCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCHHHH
CTAVAERYGRHPAVRFWQTDNEMGCHQTVVSYSPAALARFREWLKARYGTIDALNRAWGT
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCE
VFWSMEYRDFDEIDAPVGTVTEAHPTHRLDYRRFASDEVVRYHRMQVDVIRAHSPGRPVA
EEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHEEEEEECCCCCCHHH
HNFMQLFTDFDHYAVARDLDVATWDSYPLGALEEHWFAPDVKARWLRTGHPDFASFNHDL
HHHHHHHHCCCHHHHHHCCCEEECCCCCCCCHHHHCCCCCCHHHHHHCCCCCHHHCCHHH
YRGMSKLPFWVMEQQPGPVNWAHWNPAPLPGMVRLWSWEAFAHGAGCVSYFRWRQAPFAQ
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHCCCCHH
EQMHAGLHTPDDRLDEGGREAERVAHEIAVVHGADAHADAAVHARVALVFDYEAKWLLDV
HHHHCCCCCCHHHHHCCCHHHHHHHHHHHEEECCCCCCCCEEEEEEEEEEECCCEEEEEE
QPQGADFQYARIAFEYYSALRALGLDVDVISAQAPLDGYRLVVVPPLPIVPDDFAARLAA
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHC
SGAHAVFGPRTGSKTVDLQIPPTLPPGALASILPLRVWRVESLRPNVTERVDGALVDGTP
CCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHEEHHCCCCCHHHHCCCEEECCCC
FAGHARHWRDLVELRDAQRTVVRARFADDHPAWLSHGTLHYWAALFDDATTAQLFASVAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHH
EAGLAPTPLADGVRISRRGGLTYVFNYGDTVHTIDAVPPAAFVVGSADVEPRGVAVYRSP
HCCCCCCCCCCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEEECCCCCCCCEEEEECC
V
C
>Mature Secondary Structure
MRVGVCYYPEHWPESMWADDARRMKALGIAQVRIAEFAWSRIEPSPGEYDWGWLDRAVDV
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LAAAGLDIVMCTPTATPPKWLIDRHPEILAIGADGRPRTFGSRRHYDFSSPVYYDASRQI
HHHCCCEEEEECCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCHHHH
CTAVAERYGRHPAVRFWQTDNEMGCHQTVVSYSPAALARFREWLKARYGTIDALNRAWGT
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCE
VFWSMEYRDFDEIDAPVGTVTEAHPTHRLDYRRFASDEVVRYHRMQVDVIRAHSPGRPVA
EEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHEEEEEECCCCCCHHH
HNFMQLFTDFDHYAVARDLDVATWDSYPLGALEEHWFAPDVKARWLRTGHPDFASFNHDL
HHHHHHHHCCCHHHHHHCCCEEECCCCCCCCHHHHCCCCCCHHHHHHCCCCCHHHCCHHH
YRGMSKLPFWVMEQQPGPVNWAHWNPAPLPGMVRLWSWEAFAHGAGCVSYFRWRQAPFAQ
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHCCCCHH
EQMHAGLHTPDDRLDEGGREAERVAHEIAVVHGADAHADAAVHARVALVFDYEAKWLLDV
HHHHCCCCCCHHHHHCCCHHHHHHHHHHHEEECCCCCCCCEEEEEEEEEEECCCEEEEEE
QPQGADFQYARIAFEYYSALRALGLDVDVISAQAPLDGYRLVVVPPLPIVPDDFAARLAA
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHC
SGAHAVFGPRTGSKTVDLQIPPTLPPGALASILPLRVWRVESLRPNVTERVDGALVDGTP
CCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHEEHHCCCCCHHHHCCCEEECCCC
FAGHARHWRDLVELRDAQRTVVRARFADDHPAWLSHGTLHYWAALFDDATTAQLFASVAA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHH
EAGLAPTPLADGVRISRRGGLTYVFNYGDTVHTIDAVPPAAFVVGSADVEPRGVAVYRSP
HCCCCCCCCCCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEEECCCCCCCCEEEEECC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9757561; 12215416 [H]