Click here to switch to the map view.

The map label for this gene is araF [H]

Identifier: 189354066

GI number: 189354066

Start: 2399071

End: 2400075

Strand: Reverse

Name: araF [H]

Synonym: BMULJ_05319

Alternate gene names: 189354066

Gene position: 2400075-2399071 (Counterclockwise)

Preceding gene: 189354067

Following gene: 189354065

Centisome position: 97.04

GC content: 67.06

Gene sequence:

>1005_bases
ATGTTTCGCACGAATCGCAGAACCCTGTTGCGCGCGCTGCTGGCCGCGCTGTGCATCGCGCCGCTCGGCATGCAGGGCAC
GGCCCGCGCCGATGCGCCGCTGAAGATCGGTTTCCTCGTCAAGATGCCCGAGCAGGCCTGGTTCATCAACGAACAGAACG
CGGCGGCCGCGCTCGGTCAGAAGGAGAATTTCTCGGTCGTGAAGATCGGCACGCCGGACGGCGAGAAGGTGCTGGCCGCG
ATCGACAACCTCGGCTCGCAAGGCGCGCAGGGCTTCGTGATCTGCGCGCCCGACGTGCGGCTCGGGCCGGCGATCGCCGC
GCGCGCGAAGCGCTACAACATGAAGTTCGTGACCGTCGACGACCAGCTCGTCGATTCGACCGGCAAGCCGCTCGCGAACG
TGCCGCACCTCGGCATGTCGGCGACGAAGATCGGCAACCAGGTCGGCCAGGCGATCGCCGACGAGATGAAGCGGCGCGGC
TGGAAGCCGGAGGAAGTCGGCGCGCTGCGCATCACGAACTACGAACTGCCGACCGCGAAGCTGCGCACTGACGGCGCCAC
GCAGGCGCTGCTCGCGAACGGCTTCCGCAAGGAGAACATCTTCGACGCACCGCAGAAGACGACCGACGACGAAGGCGGCT
TCAGCGCGGCGGCGCCGGTGCTCGCGCGCCATCCGAACGTGAAGAAGTGGGTGATCTACGCGCTGAACGAGGAAACCGTG
CTCGGCGCCGTGCGCGCGACCGAACAGCTGCACATTCCGGCGGCCGACGTGATCGGCGTCGGCATCAACGGTGCGGGCGA
GGCGTTCGCGGAATTCCAGAAGAAGGAGCCGACCGGCTTCTTCGGCACGATCGCGGTCAGCTCGACGAATCACGGCAAGG
AAAGCACGCAGAACCTCGTCGACTGGATCCGCAACGGCAAGATGCCGCCCGCCGATACGCAGACGAGCGGCAAGCTGATG
ACGCGCAGCAACTGGCAGGCGGTGCGCGCCGAGCTCGGCATCTGA

Upstream 100 bases:

>100_bases
GCCGCGTCGACACCGAAGCGTTCGGCCGCTGAGCGCGGCGGCCGCACGCGCATCACCTTCGAGAACCACCACACACAGAG
TCATACAAGGAGACAACGAC

Downstream 100 bases:

>100_bases
GCGGCGCGAGCGAGGACGCGATGACGACGCAGACGATGACGGCCGCCGCCTCTCCCGCCGCGGCTGCGGCGCCCGTGCAG
CGCGATGCGCTGCTCGCGCT

Product: ABC-type sugar transport system periplasmic component

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MFRTNRRTLLRALLAALCIAPLGMQGTARADAPLKIGFLVKMPEQAWFINEQNAAAALGQKENFSVVKIGTPDGEKVLAA
IDNLGSQGAQGFVICAPDVRLGPAIAARAKRYNMKFVTVDDQLVDSTGKPLANVPHLGMSATKIGNQVGQAIADEMKRRG
WKPEEVGALRITNYELPTAKLRTDGATQALLANGFRKENIFDAPQKTTDDEGGFSAAAPVLARHPNVKKWVIYALNEETV
LGAVRATEQLHIPAADVIGVGINGAGEAFAEFQKKEPTGFFGTIAVSSTNHGKESTQNLVDWIRNGKMPPADTQTSGKLM
TRSNWQAVRAELGI

Sequences:

>Translated_334_residues
MFRTNRRTLLRALLAALCIAPLGMQGTARADAPLKIGFLVKMPEQAWFINEQNAAAALGQKENFSVVKIGTPDGEKVLAA
IDNLGSQGAQGFVICAPDVRLGPAIAARAKRYNMKFVTVDDQLVDSTGKPLANVPHLGMSATKIGNQVGQAIADEMKRRG
WKPEEVGALRITNYELPTAKLRTDGATQALLANGFRKENIFDAPQKTTDDEGGFSAAAPVLARHPNVKKWVIYALNEETV
LGAVRATEQLHIPAADVIGVGINGAGEAFAEFQKKEPTGFFGTIAVSSTNHGKESTQNLVDWIRNGKMPPADTQTSGKLM
TRSNWQAVRAELGI
>Mature_334_residues
MFRTNRRTLLRALLAALCIAPLGMQGTARADAPLKIGFLVKMPEQAWFINEQNAAAALGQKENFSVVKIGTPDGEKVLAA
IDNLGSQGAQGFVICAPDVRLGPAIAARAKRYNMKFVTVDDQLVDSTGKPLANVPHLGMSATKIGNQVGQAIADEMKRRG
WKPEEVGALRITNYELPTAKLRTDGATQALLANGFRKENIFDAPQKTTDDEGGFSAAAPVLARHPNVKKWVIYALNEETV
LGAVRATEQLHIPAADVIGVGINGAGEAFAEFQKKEPTGFFGTIAVSSTNHGKESTQNLVDWIRNGKMPPADTQTSGKLM
TRSNWQAVRAELGI

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=314, Percent_Identity=46.4968152866242, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35871; Mature: 35871

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTNRRTLLRALLAALCIAPLGMQGTARADAPLKIGFLVKMPEQAWFINEQNAAAALGQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHEEECCCCHHHHCCC
KENFSVVKIGTPDGEKVLAAIDNLGSQGAQGFVICAPDVRLGPAIAARAKRYNMKFVTVD
CCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEEC
DQLVDSTGKPLANVPHLGMSATKIGNQVGQAIADEMKRRGWKPEEVGALRITNYELPTAK
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCEEEEECCCCCCCE
LRTDGATQALLANGFRKENIFDAPQKTTDDEGGFSAAAPVLARHPNVKKWVIYALNEETV
ECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCHHHHCCCCCCEEEEEEECCCCE
LGAVRATEQLHIPAADVIGVGINGAGEAFAEFQKKEPTGFFGTIAVSSTNHGKESTQNLV
EHHHHHCCEECCCCHHEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHH
DWIRNGKMPPADTQTSGKLMTRSNWQAVRAELGI
HHHHCCCCCCCCCCCCCCEEECCCHHHHHHHCCC
>Mature Secondary Structure
MFRTNRRTLLRALLAALCIAPLGMQGTARADAPLKIGFLVKMPEQAWFINEQNAAAALGQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCHHEEECCCCHHHHCCC
KENFSVVKIGTPDGEKVLAAIDNLGSQGAQGFVICAPDVRLGPAIAARAKRYNMKFVTVD
CCCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEEC
DQLVDSTGKPLANVPHLGMSATKIGNQVGQAIADEMKRRGWKPEEVGALRITNYELPTAK
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCEEEEECCCCCCCE
LRTDGATQALLANGFRKENIFDAPQKTTDDEGGFSAAAPVLARHPNVKKWVIYALNEETV
ECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCHHHHCCCCCCEEEEEEECCCCE
LGAVRATEQLHIPAADVIGVGINGAGEAFAEFQKKEPTGFFGTIAVSSTNHGKESTQNLV
EHHHHHCCEECCCCHHEEEECCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHH
DWIRNGKMPPADTQTSGKLMTRSNWQAVRAELGI
HHHHCCCCCCCCCCCCCCEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]