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The map label for this gene is hisJ [H]

Identifier: 189354045

GI number: 189354045

Start: 2372001

End: 2372786

Strand: Reverse

Name: hisJ [H]

Synonym: BMULJ_05298

Alternate gene names: 189354045

Gene position: 2372786-2372001 (Counterclockwise)

Preceding gene: 189354047

Following gene: 189354044

Centisome position: 95.94

GC content: 65.14

Gene sequence:

>786_bases
ATGAACCGGATTGCCAAGCTTCTCGCCACCGCACTCGCCTGCGCGCCGCTCGTGTCGTTCGCGCAGGACACGTCGACGAT
CCGCTGGGGCATCGACCCGACGTATCCGCCGTTCGAGGCGAAACAGCCGGACGGCTCGCTGACCGGCTTCGACATCGACC
TGCGCGACGCGATCTGCGCGCAGCTGCATGCGAAATGCGTATGGGTCGAGCAGAACTTCGACGGGATGATTCCGGCGCTG
CGCGCGCGCAAGTTCGACGTGATCATGTCGGCGATGACGGCAACCGAAGAACGGCTGAAGCAGATCGACTTCTCGAACAA
GCTCTACGCGTCGCCGGCCGCGCTCGTCGCGCCGGCCGGCTCGAAGCTGCAGTCGACGGCCGCGTCGCTCGCGGGCAAGC
GCGTCGGGATCATCCAGGGCACGACGCAGGACATGTACGCGAAGGCCGAATGGGCGCCGAAGGGCGTGACGATCGTCACG
TACCAGAACCAGGACCAGGTGTATCAGGATCTCGTGAACGGCCGCCTCGACGCGACGTTCCAGGACAAGACGCAGGCCGG
CTATTCGTTTCTGAAGACCGAGCGCGGCAAGCACTTCGCGTTCGCCGGCGACGACATCACCGATTCGCGGATCACGGGTT
TCGGCGTCGCGATGGGGCTGCGCAAGGGCGATGCGGCGCTCAAGGCGCGGCTGAACGACGCGATCGCGGCGATCCGCGCG
AACGGCACCTACCAGAAGATCGCCGCGAAATACTTCGACTTCGACATCTACGGCGCGAAGCAGTAA

Upstream 100 bases:

>100_bases
ATGCCCTGTTTCGCGCACCGCGCCGGCATCGGAATCCGGCGCGGCGTATCCACCGGACGTCGCGCGCCCGCGCGCCGCGT
CGAGACCCAAAGGATCAGCC

Downstream 100 bases:

>100_bases
GCGGCGCGCATTCAACCGACTGCAGGCAGACCATGACGACGAGCATCTCCGAGCGTGTCGCGCAAGCCGTGACGCCCGAC
CTGATCGAAGCGTTCCGCGC

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNRIAKLLATALACAPLVSFAQDTSTIRWGIDPTYPPFEAKQPDGSLTGFDIDLRDAICAQLHAKCVWVEQNFDGMIPAL
RARKFDVIMSAMTATEERLKQIDFSNKLYASPAALVAPAGSKLQSTAASLAGKRVGIIQGTTQDMYAKAEWAPKGVTIVT
YQNQDQVYQDLVNGRLDATFQDKTQAGYSFLKTERGKHFAFAGDDITDSRITGFGVAMGLRKGDAALKARLNDAIAAIRA
NGTYQKIAAKYFDFDIYGAKQ

Sequences:

>Translated_261_residues
MNRIAKLLATALACAPLVSFAQDTSTIRWGIDPTYPPFEAKQPDGSLTGFDIDLRDAICAQLHAKCVWVEQNFDGMIPAL
RARKFDVIMSAMTATEERLKQIDFSNKLYASPAALVAPAGSKLQSTAASLAGKRVGIIQGTTQDMYAKAEWAPKGVTIVT
YQNQDQVYQDLVNGRLDATFQDKTQAGYSFLKTERGKHFAFAGDDITDSRITGFGVAMGLRKGDAALKARLNDAIAAIRA
NGTYQKIAAKYFDFDIYGAKQ
>Mature_261_residues
MNRIAKLLATALACAPLVSFAQDTSTIRWGIDPTYPPFEAKQPDGSLTGFDIDLRDAICAQLHAKCVWVEQNFDGMIPAL
RARKFDVIMSAMTATEERLKQIDFSNKLYASPAALVAPAGSKLQSTAASLAGKRVGIIQGTTQDMYAKAEWAPKGVTIVT
YQNQDQVYQDLVNGRLDATFQDKTQAGYSFLKTERGKHFAFAGDDITDSRITGFGVAMGLRKGDAALKARLNDAIAAIRA
NGTYQKIAAKYFDFDIYGAKQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=50, Blast_Score=260, Evalue=6e-71,
Organism=Escherichia coli, GI1788649, Length=240, Percent_Identity=48.75, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=37.3015873015873, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=33.7349397590361, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788228, Length=254, Percent_Identity=31.496062992126, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=32.5396825396825, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28451; Mature: 28451

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIAKLLATALACAPLVSFAQDTSTIRWGIDPTYPPFEAKQPDGSLTGFDIDLRDAICA
CHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHH
QLHAKCVWVEQNFDGMIPALRARKFDVIMSAMTATEERLKQIDFSNKLYASPAALVAPAG
HHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEECCCC
SKLQSTAASLAGKRVGIIQGTTQDMYAKAEWAPKGVTIVTYQNQDQVYQDLVNGRLDATF
CHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEE
QDKTQAGYSFLKTERGKHFAFAGDDITDSRITGFGVAMGLRKGDAALKARLNDAIAAIRA
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEC
NGTYQKIAAKYFDFDIYGAKQ
CCCHHHHHHHHHCEEEECCCC
>Mature Secondary Structure
MNRIAKLLATALACAPLVSFAQDTSTIRWGIDPTYPPFEAKQPDGSLTGFDIDLRDAICA
CHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHH
QLHAKCVWVEQNFDGMIPALRARKFDVIMSAMTATEERLKQIDFSNKLYASPAALVAPAG
HHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEECCCC
SKLQSTAASLAGKRVGIIQGTTQDMYAKAEWAPKGVTIVTYQNQDQVYQDLVNGRLDATF
CHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEE
QDKTQAGYSFLKTERGKHFAFAGDDITDSRITGFGVAMGLRKGDAALKARLNDAIAAIRA
CHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEEC
NGTYQKIAAKYFDFDIYGAKQ
CCCHHHHHHHHHCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]