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The map label for this gene is spr [H]

Identifier: 189354042

GI number: 189354042

Start: 2369533

End: 2370270

Strand: Direct

Name: spr [H]

Synonym: BMULJ_05295

Alternate gene names: 189354042

Gene position: 2369533-2370270 (Clockwise)

Preceding gene: 189354028

Following gene: 189354043

Centisome position: 95.81

GC content: 71.95

Gene sequence:

>738_bases
GTGCGCGCCATCGTCACCGGTCATACGCGCGGGCTCGGCGCCGCGCTCGCCACGCAATTGCTCGATCAGGGCCTCGCCGT
GCTCGGCGTGTCGCGCAGCCGTCATCCCTCGCTCGCGGCCGCGTCGCACGCGCCGTTCGCCGAGATCGAGCTCGATCTGT
CGGACACGGCCGCCGTCGCCGCATGGCTCGCCGGCGATACGCTGCGACGCTTCGTCGATGGCGCATCGCTCGTGCTGCTG
TTCAACAACGCGGGGATCGTCGATCCGATCGGCCCGCTCGACGTGCAGGACGCGAACACGGTCGCACGCGCGGTCAACCT
CAACGTCGCGGCGCCGCTGATGCTGTCGGCCGCGCTCGCGCAAGCCGTCTCCGCGCCGACCGAGTGCCGGATCGTGCACG
TATCGAGCGGCGCCGCACGCAATGCCTACGCGGGCTGGAGCGTCTATTGCGCGACCAAGGCCGCGCTCGATCACCATGCG
CGCGCGGTCGCGCTCGACGCGAATCGCGCGCTGCGGATTGGCAGCGTCGCGCCGGGCGTCGTCGACACCGGCATGCAGGC
GACGATCCGCGCAACCGGCGACGACCGCTTTCCGATGCGCGACAAGTTCGAGCAGTTGAAGGCGAGCGGCGCGCTGTCGA
CGCCCGAGGCGGCAGCGCGGCAGCTGATCCGCTACGCGCTGAGCGACGCGTTCGGCGCGACGCCGACGGCCGACGTGCGC
GAGTTGCCGCCCGCCTGA

Upstream 100 bases:

>100_bases
GCGCCGCGTACCGTTTCGTTTTCGCGCGACGCCGCCGCGCGTGCGCGCTCCACGCTCTTCACCGTTCACCGACATGACTT
CATCCGCCGGCGCACCGAAC

Downstream 100 bases:

>100_bases
TCCGCTAGAATCCGTCGATCGCCTTTGCCCGAGAGTTCCCCGATGGTCATGAAGACCACCGCGCCGTTCCAGCAGATCAA
GACGCTCGTTCGCCAGAAAA

Product: short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRAIVTGHTRGLGAALATQLLDQGLAVLGVSRSRHPSLAAASHAPFAEIELDLSDTAAVAAWLAGDTLRRFVDGASLVLL
FNNAGIVDPIGPLDVQDANTVARAVNLNVAAPLMLSAALAQAVSAPTECRIVHVSSGAARNAYAGWSVYCATKAALDHHA
RAVALDANRALRIGSVAPGVVDTGMQATIRATGDDRFPMRDKFEQLKASGALSTPEAAARQLIRYALSDAFGATPTADVR
ELPPA

Sequences:

>Translated_245_residues
MRAIVTGHTRGLGAALATQLLDQGLAVLGVSRSRHPSLAAASHAPFAEIELDLSDTAAVAAWLAGDTLRRFVDGASLVLL
FNNAGIVDPIGPLDVQDANTVARAVNLNVAAPLMLSAALAQAVSAPTECRIVHVSSGAARNAYAGWSVYCATKAALDHHA
RAVALDANRALRIGSVAPGVVDTGMQATIRATGDDRFPMRDKFEQLKASGALSTPEAAARQLIRYALSDAFGATPTADVR
ELPPA
>Mature_245_residues
MRAIVTGHTRGLGAALATQLLDQGLAVLGVSRSRHPSLAAASHAPFAEIELDLSDTAAVAAWLAGDTLRRFVDGASLVLL
FNNAGIVDPIGPLDVQDANTVARAVNLNVAAPLMLSAALAQAVSAPTECRIVHVSSGAARNAYAGWSVYCATKAALDHHA
RAVALDANRALRIGSVAPGVVDTGMQATIRATGDDRFPMRDKFEQLKASGALSTPEAAARQLIRYALSDAFGATPTADVR
ELPPA

Specific function: Reduces benzil stereospecifically to (S)-benzoin. Can also reduces 1-phenyl-1,2-propanedione, 1,4-naphthoquinone, 1-(4- methyl-phenyl)-2-phenyl-ethane-1,2-dione, 1-(4-fluoro-phenyl)-2- phenyl-ethane-1,2-dione, methyl benzoylformate, p- nitrobenzaldehyde i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=169, Percent_Identity=33.1360946745562, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787335, Length=199, Percent_Identity=27.6381909547739, Blast_Score=60, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322226, Length=213, Percent_Identity=29.5774647887324, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322227, Length=245, Percent_Identity=30.6122448979592, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24643142, Length=189, Percent_Identity=35.4497354497354, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI23397609, Length=187, Percent_Identity=31.5508021390374, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28571526, Length=219, Percent_Identity=29.6803652968037, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 25321; Mature: 25321

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIVTGHTRGLGAALATQLLDQGLAVLGVSRSRHPSLAAASHAPFAEIELDLSDTAAVA
CCEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
AWLAGDTLRRFVDGASLVLLFNNAGIVDPIGPLDVQDANTVARAVNLNVAAPLMLSAALA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
QAVSAPTECRIVHVSSGAARNAYAGWSVYCATKAALDHHARAVALDANRALRIGSVAPGV
HHHCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHHCCEEEEECCCCEEEECCCCCCH
VDTGMQATIRATGDDRFPMRDKFEQLKASGALSTPEAAARQLIRYALSDAFGATPTADVR
HCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
ELPPA
CCCCC
>Mature Secondary Structure
MRAIVTGHTRGLGAALATQLLDQGLAVLGVSRSRHPSLAAASHAPFAEIELDLSDTAAVA
CCEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
AWLAGDTLRRFVDGASLVLLFNNAGIVDPIGPLDVQDANTVARAVNLNVAAPLMLSAALA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
QAVSAPTECRIVHVSSGAARNAYAGWSVYCATKAALDHHARAVALDANRALRIGSVAPGV
HHHCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHHCCEEEEECCCCEEEECCCCCCH
VDTGMQATIRATGDDRFPMRDKFEQLKASGALSTPEAAARQLIRYALSDAFGATPTADVR
HCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
ELPPA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11745140; 11796169 [H]