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The map label for this gene is dfrA [H]

Identifier: 189354016

GI number: 189354016

Start: 2344549

End: 2345556

Strand: Direct

Name: dfrA [H]

Synonym: BMULJ_05269

Alternate gene names: 189354016

Gene position: 2344549-2345556 (Clockwise)

Preceding gene: 189354012

Following gene: 189354017

Centisome position: 94.8

GC content: 70.34

Gene sequence:

>1008_bases
ATGACTGATACCTCCCGCGATCTCGTTCTCGTCACCGGCGCGTCCGGTTTCGTCGGCTCGGCTGTCGCGCGCATCGCGCA
GCAGAAAGGCTATGCGGTGCGTGTGCTGGTGCGCCCGACGAGCCCGCGCACGAACGTCGCCGATCTCGACGCCGAGATCG
TCACCGGCGACATGCGCGACGAGACGTCGATGCGCGCCGCGCTGCGCGGCGTGCGCTACCTGCTGCACGTCGCGGCCGAC
TACCGGCTGTGGGCGCCGGACCCGCACGAGATCGAACGCGCGAACCTCGAAGGCGCCGTCGCGACGATGCGCGCCGCGCG
CGCCGAAGGCGTCGAGCGGATCGTCTATACGAGCAGCGTCGCGACGCTGAAGGTCACGAGCGCCGGCGATCCGGCCGACG
AGAACCGGCCGCTCACGCCCGAGCAGGCGATCGGCGTGTACAAGCGCAGCAAGGTGCTCGCCGAGCGTGCGGTCGAGCGG
ATGATCGCCGACGAAGGGCTGCCCGCGGTGATCGTCAACCCGTCGACGCCGATCGGCCCGCGCGACGTGAAGCCGACGCC
GACCGGCCGCATCATCGTCGAGGCCGCGCTCGGCAAGATTCCGGCGTTCGTCGATACGGGGCTGAACCTCGTGCATGTCG
ACGACGTCGCGCACGGCCACTTCCTCGCGCTCGAGCGCGGCCGGATCGGCGAGCGCTACATCCTGGGCGGCGAGAACCTG
CCGCTGCAGCAGATGCTCGCCGACATCGCGCAGATGACGGGCCGCAAGGCGCCGACGATCGCGCTGCCGCGCTGGCCGTT
GTACCCGCTCGCGGTCGGCGCCGAGGCGGTCGCGAAGTTCACGAAGAAGGAGCCGTTCGTCACGGTCGACGGGCTGCGGA
TGTCGAAGAACAAGATGTATTTCACGTCCGCGAAGGCCGAGCGCGAACTCGGCTACCGCGCGCGGCCGTACCGCGACGGG
CTGCGCGACGCGCTCGACTGGTTCCGCGGCGCGGGCTACCTGAAGTAG

Upstream 100 bases:

>100_bases
TTCTCACACGGGGTCGGCCGGCCGCCGCGCGCGCCGAACACGTACAATCTCGCCGACGCTCAACCGCCGCGCCGTTCGCG
CGCGGCCAAGAAGGAATGGC

Downstream 100 bases:

>100_bases
CACAAAGCGGCGCGCAGCGCGCCGGATTGCGGACTTTGTTACACGTGATGCGCGGACCGCGCGCGGCTCAGCGGGTAAAA
TCGCGGGTCCTACGTAGAGA

Product: dihydroflavonol-4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTDTSRDLVLVTGASGFVGSAVARIAQQKGYAVRVLVRPTSPRTNVADLDAEIVTGDMRDETSMRAALRGVRYLLHVAAD
YRLWAPDPHEIERANLEGAVATMRAARAEGVERIVYTSSVATLKVTSAGDPADENRPLTPEQAIGVYKRSKVLAERAVER
MIADEGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAALGKIPAFVDTGLNLVHVDDVAHGHFLALERGRIGERYILGGENL
PLQQMLADIAQMTGRKAPTIALPRWPLYPLAVGAEAVAKFTKKEPFVTVDGLRMSKNKMYFTSAKAERELGYRARPYRDG
LRDALDWFRGAGYLK

Sequences:

>Translated_335_residues
MTDTSRDLVLVTGASGFVGSAVARIAQQKGYAVRVLVRPTSPRTNVADLDAEIVTGDMRDETSMRAALRGVRYLLHVAAD
YRLWAPDPHEIERANLEGAVATMRAARAEGVERIVYTSSVATLKVTSAGDPADENRPLTPEQAIGVYKRSKVLAERAVER
MIADEGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAALGKIPAFVDTGLNLVHVDDVAHGHFLALERGRIGERYILGGENL
PLQQMLADIAQMTGRKAPTIALPRWPLYPLAVGAEAVAKFTKKEPFVTVDGLRMSKNKMYFTSAKAERELGYRARPYRDG
LRDALDWFRGAGYLK
>Mature_334_residues
TDTSRDLVLVTGASGFVGSAVARIAQQKGYAVRVLVRPTSPRTNVADLDAEIVTGDMRDETSMRAALRGVRYLLHVAADY
RLWAPDPHEIERANLEGAVATMRAARAEGVERIVYTSSVATLKVTSAGDPADENRPLTPEQAIGVYKRSKVLAERAVERM
IADEGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAALGKIPAFVDTGLNLVHVDDVAHGHFLALERGRIGERYILGGENLP
LQQMLADIAQMTGRKAPTIALPRWPLYPLAVGAEAVAKFTKKEPFVTVDGLRMSKNKMYFTSAKAERELGYRARPYRDGL
RDALDWFRGAGYLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=356, Percent_Identity=24.7191011235955, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI193211614, Length=321, Percent_Identity=24.9221183800623, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI8393516, Length=321, Percent_Identity=24.9221183800623, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310132178, Length=359, Percent_Identity=25.6267409470752, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310113012, Length=359, Percent_Identity=25.6267409470752, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI239745448, Length=359, Percent_Identity=25.6267409470752, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71987463, Length=332, Percent_Identity=29.8192771084337, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36553; Mature: 36422

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTSRDLVLVTGASGFVGSAVARIAQQKGYAVRVLVRPTSPRTNVADLDAEIVTGDMRD
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCEEEECCCCC
ETSMRAALRGVRYLLHVAADYRLWAPDPHEIERANLEGAVATMRAARAEGVERIVYTSSV
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCE
ATLKVTSAGDPADENRPLTPEQAIGVYKRSKVLAERAVERMIADEGLPAVIVNPSTPIGP
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
RDVKPTPTGRIIVEAALGKIPAFVDTGLNLVHVDDVAHGHFLALERGRIGERYILGGENL
CCCCCCCCCCEEEEEHHCCCCHHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCC
PLQQMLADIAQMTGRKAPTIALPRWPLYPLAVGAEAVAKFTKKEPFVTVDGLRMSKNKMY
CHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHCHHHHHHHHCCCCCEEECCCEECCCEEE
FTSAKAERELGYRARPYRDGLRDALDWFRGAGYLK
EECCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDTSRDLVLVTGASGFVGSAVARIAQQKGYAVRVLVRPTSPRTNVADLDAEIVTGDMRD
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCEEEECCCCC
ETSMRAALRGVRYLLHVAADYRLWAPDPHEIERANLEGAVATMRAARAEGVERIVYTSSV
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHEEEEECCE
ATLKVTSAGDPADENRPLTPEQAIGVYKRSKVLAERAVERMIADEGLPAVIVNPSTPIGP
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
RDVKPTPTGRIIVEAALGKIPAFVDTGLNLVHVDDVAHGHFLALERGRIGERYILGGENL
CCCCCCCCCCEEEEEHHCCCCHHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCC
PLQQMLADIAQMTGRKAPTIALPRWPLYPLAVGAEAVAKFTKKEPFVTVDGLRMSKNKMY
CHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHCHHHHHHHHCCCCCEEECCCEECCCEEE
FTSAKAERELGYRARPYRDGLRDALDWFRGAGYLK
EECCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]