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The map label for this gene is fhlA [H]

Identifier: 189353996

GI number: 189353996

Start: 2320597

End: 2322093

Strand: Reverse

Name: fhlA [H]

Synonym: BMULJ_05249

Alternate gene names: 189353996

Gene position: 2322093-2320597 (Counterclockwise)

Preceding gene: 189353999

Following gene: 189353995

Centisome position: 93.89

GC content: 69.74

Gene sequence:

>1497_bases
ATGATGAACGACTGGACCCGGCTGCCCGCCAACTACGGCGACGTGCTGCGGCGCGCCGCCGAGTCGCTGTTTCGCACGTT
CGAGGATTCGAGCGCGGGCACGGTGGTCGTCGATCGCGATGCGCGGATCGTCTGGATGAACGAGCGCTACGCGGCGCGTT
TCGGCTTTGCGGATCCGCAGCAGGCCGTCGGCCTCGACTGCGAGGCCGTGATTCCGAACAGCCTGCTGCGCGAAGTCGTG
TCGAGCGGCCAGCCGATCCTGCTCGACATCATGGAGACCGGCCGCGAACCGCTCGTCGTCACGCGGCTGCCGCTGAAGAA
CGAGGCCGGCGAGACGGTCGGCGCGATCGGCTTCGCGCTGTTCGATCAGTTGAAGACGCTCACGCCGATCTTCTCGCGCT
ACGCGCAGCTTCAGCAGCAGCTGATCGCGACGCAGCGCTCGCTCGCGCAGGCGCGCCGCGCGAAGTACACGTTCGCGAGC
TTCGTCGGCACGAGCGCCGCGAGCCTCGAGACGAAACGACAGGCACGCCGCGCCGCGCAAGTCGATTCGCCCGTGCTGCT
GCTCGGCGAGACGGGCACCGGCAAGGAGCTGCTCGCGCATGCGATTCACGCGGCGTCGGCGCGTGCGCTGCAGCCGCTCG
TGACCGTCAACGTCGCGGCGATTCCCGACACGCTGCTGGAGACCGAGTTCTTCGGCGCCGCGCCCGGCGCGTATACCGGC
GCCGATCGCAAGGGCCGCGTCGGCAAGTTCGAGCTGGCCGATCGCGGCACGCTGTTTCTCGACGAGATCGGCGACATGCC
GTTGCCGCTGCAGGGCAAGTTGCTGCGCGTGCTGCAGGACAAGGAATTCGAACCGGTCGGGTCGAACCGCATCGTGCGCG
CGGACGTGCGCATCATTGCGGCGACGTCGGCCGATCTGCCGGCGCTCGTCGCGGCCGGACGCTTTCGCGCCGATCTCTAT
TACCGGCTCAATGTGCTGACGATTCATGCGCCGCCGCTGCGCGAGCGCGCGTCGGACATTGCGGCGCTCGTCTATGTGAC
GCTCGAGGAGCTCGCTGCGCAGCACGGGCGCGCCGCGCACTGCGAGCTGACCGACGACGCGTTGCGGATGCTCTGCGCCT
ATCCGTGGCCGGGCAACGTCCGCGAGCTGCGCAACACGCTCGAGCGCGCGCTGATGCTGTCGGATCGCGCGATCATCGAT
GCACGTGCGCTCGCGCCGTTTCTCGGACCGATGCGCGTGCATGCGGACGCCGCGCCGGCCGTGCCGACGGCTGCCGTGAC
GGCGGGCGGCGGGGGCGTCGGTTCCAGATCCGGCTCCGGCGACCCGCATGCCGGGCAGCACGTGTCGTATGCGGACGCGC
TCGCGGCGTGGGAACGCCAGTTCTTCGCCGACGCGCTGGCCGCGTGCGACGGCAAGGTGGTGGAGGCCGCCGCGCGGATC
GGCATCGGACGCGCAACGCTGTACAAGAAGCTCGCGGCGCTCGGTATCGGTCGATAG

Upstream 100 bases:

>100_bases
AGGGTCTTCCCGGACAAGGCAATCCGGCGTGTCTCCAAAATGAGATAATCCATCCCGATACGGAGACATCCGACCGAAGA
TACACGGAGACGACGACACG

Downstream 100 bases:

>100_bases
GCCGCTACCGACCACGCGGGTGCGCGCGCCTGCATGGTGCGCATCGGCTATGGGAACGATCGCGAAACACAGGCCGGCCG
GCAAGCGCGTGCGCATCGGC

Product: putative sigma54-dependent transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MMNDWTRLPANYGDVLRRAAESLFRTFEDSSAGTVVVDRDARIVWMNERYAARFGFADPQQAVGLDCEAVIPNSLLREVV
SSGQPILLDIMETGREPLVVTRLPLKNEAGETVGAIGFALFDQLKTLTPIFSRYAQLQQQLIATQRSLAQARRAKYTFAS
FVGTSAASLETKRQARRAAQVDSPVLLLGETGTGKELLAHAIHAASARALQPLVTVNVAAIPDTLLETEFFGAAPGAYTG
ADRKGRVGKFELADRGTLFLDEIGDMPLPLQGKLLRVLQDKEFEPVGSNRIVRADVRIIAATSADLPALVAAGRFRADLY
YRLNVLTIHAPPLRERASDIAALVYVTLEELAAQHGRAAHCELTDDALRMLCAYPWPGNVRELRNTLERALMLSDRAIID
ARALAPFLGPMRVHADAAPAVPTAAVTAGGGGVGSRSGSGDPHAGQHVSYADALAAWERQFFADALAACDGKVVEAAARI
GIGRATLYKKLAALGIGR

Sequences:

>Translated_498_residues
MMNDWTRLPANYGDVLRRAAESLFRTFEDSSAGTVVVDRDARIVWMNERYAARFGFADPQQAVGLDCEAVIPNSLLREVV
SSGQPILLDIMETGREPLVVTRLPLKNEAGETVGAIGFALFDQLKTLTPIFSRYAQLQQQLIATQRSLAQARRAKYTFAS
FVGTSAASLETKRQARRAAQVDSPVLLLGETGTGKELLAHAIHAASARALQPLVTVNVAAIPDTLLETEFFGAAPGAYTG
ADRKGRVGKFELADRGTLFLDEIGDMPLPLQGKLLRVLQDKEFEPVGSNRIVRADVRIIAATSADLPALVAAGRFRADLY
YRLNVLTIHAPPLRERASDIAALVYVTLEELAAQHGRAAHCELTDDALRMLCAYPWPGNVRELRNTLERALMLSDRAIID
ARALAPFLGPMRVHADAAPAVPTAAVTAGGGGVGSRSGSGDPHAGQHVSYADALAAWERQFFADALAACDGKVVEAAARI
GIGRATLYKKLAALGIGR
>Mature_498_residues
MMNDWTRLPANYGDVLRRAAESLFRTFEDSSAGTVVVDRDARIVWMNERYAARFGFADPQQAVGLDCEAVIPNSLLREVV
SSGQPILLDIMETGREPLVVTRLPLKNEAGETVGAIGFALFDQLKTLTPIFSRYAQLQQQLIATQRSLAQARRAKYTFAS
FVGTSAASLETKRQARRAAQVDSPVLLLGETGTGKELLAHAIHAASARALQPLVTVNVAAIPDTLLETEFFGAAPGAYTG
ADRKGRVGKFELADRGTLFLDEIGDMPLPLQGKLLRVLQDKEFEPVGSNRIVRADVRIIAATSADLPALVAAGRFRADLY
YRLNVLTIHAPPLRERASDIAALVYVTLEELAAQHGRAAHCELTDDALRMLCAYPWPGNVRELRNTLERALMLSDRAIID
ARALAPFLGPMRVHADAAPAVPTAAVTAGGGGVGSRSGSGDPHAGQHVSYADALAAWERQFFADALAACDGKVVEAAARI
GIGRATLYKKLAALGIGR

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=372, Percent_Identity=41.1290322580645, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1788550, Length=388, Percent_Identity=37.6288659793814, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082117, Length=365, Percent_Identity=38.9041095890411, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790437, Length=332, Percent_Identity=42.7710843373494, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=38.5542168674699, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788905, Length=333, Percent_Identity=39.6396396396396, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1790299, Length=483, Percent_Identity=36.231884057971, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=46.8253968253968, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=264, Percent_Identity=45.4545454545455, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=44.0677966101695, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=43.2773109243698, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081858, Length=348, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789828, Length=376, Percent_Identity=30.3191489361702, Blast_Score=124, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53558; Mature: 53558

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNDWTRLPANYGDVLRRAAESLFRTFEDSSAGTVVVDRDARIVWMNERYAARFGFADPQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCHHHHCCCCCCH
QAVGLDCEAVIPNSLLREVVSSGQPILLDIMETGREPLVVTRLPLKNEAGETVGAIGFAL
HHHCCCCHHHCCHHHHHHHHHCCCCEEEEHHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
FDQLKTLTPIFSRYAQLQQQLIATQRSLAQARRAKYTFASFVGTSAASLETKRQARRAAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VDSPVLLLGETGTGKELLAHAIHAASARALQPLVTVNVAAIPDTLLETEFFGAAPGAYTG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHEEEEHHCCHHHHHHHHCCCCCCCCCC
ADRKGRVGKFELADRGTLFLDEIGDMPLPLQGKLLRVLQDKEFEPVGSNRIVRADVRIIA
CCCCCCCCEEEECCCCCEEHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECEEEEE
ATSADLPALVAAGRFRADLYYRLNVLTIHAPPLRERASDIAALVYVTLEELAAQHGRAAH
ECCCCCHHHHHCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
CELTDDALRMLCAYPWPGNVRELRNTLERALMLSDRAIIDARALAPFLGPMRVHADAAPA
EEECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCC
VPTAAVTAGGGGVGSRSGSGDPHAGQHVSYADALAAWERQFFADALAACDGKVVEAAARI
CCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GIGRATLYKKLAALGIGR
CCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMNDWTRLPANYGDVLRRAAESLFRTFEDSSAGTVVVDRDARIVWMNERYAARFGFADPQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCHHHHCCCCCCH
QAVGLDCEAVIPNSLLREVVSSGQPILLDIMETGREPLVVTRLPLKNEAGETVGAIGFAL
HHHCCCCHHHCCHHHHHHHHHCCCCEEEEHHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
FDQLKTLTPIFSRYAQLQQQLIATQRSLAQARRAKYTFASFVGTSAASLETKRQARRAAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VDSPVLLLGETGTGKELLAHAIHAASARALQPLVTVNVAAIPDTLLETEFFGAAPGAYTG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHEEEEHHCCHHHHHHHHCCCCCCCCCC
ADRKGRVGKFELADRGTLFLDEIGDMPLPLQGKLLRVLQDKEFEPVGSNRIVRADVRIIA
CCCCCCCCEEEECCCCCEEHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECEEEEE
ATSADLPALVAAGRFRADLYYRLNVLTIHAPPLRERASDIAALVYVTLEELAAQHGRAAH
ECCCCCHHHHHCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
CELTDDALRMLCAYPWPGNVRELRNTLERALMLSDRAIIDARALAPFLGPMRVHADAAPA
EEECHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCC
VPTAAVTAGGGGVGSRSGSGDPHAGQHVSYADALAAWERQFFADALAACDGKVVEAAARI
CCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GIGRATLYKKLAALGIGR
CCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]