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The map label for this gene is prpF [H]

Identifier: 189353982

GI number: 189353982

Start: 2303001

End: 2304191

Strand: Reverse

Name: prpF [H]

Synonym: BMULJ_05235

Alternate gene names: 189353982

Gene position: 2304191-2303001 (Counterclockwise)

Preceding gene: 189353983

Following gene: 189353981

Centisome position: 93.17

GC content: 69.61

Gene sequence:

>1191_bases
ATGGCGCACATTCCTCAAATCCGGATTCCCGCGACCTACATGCGCGGCGGCACGAGCAAGGGCGTGTTCTTCCGGCTGCA
GGATCTGCCGGATGCCGCGCAGGCGCCCGGCGCCGCGCGCGACGCGCTGCTGCTGCGCGTGATCGGCAGCCCCGATCCGT
ACGGCAAGCAGATCGACGGGATGGGCGGCGCGACGTCGTCGACGAGCAAGACGGTGATCGTCTCGAAGAGCACGCGGCCC
GGCCACGACGTCGATTACCTGTTCGGGCAGGTCGCGATCGATCGCGCGTTCGTCGACTGGACCGGCAACTGCGGAAATCT
GTCCGCGGCGGTCGGCCCGTTCGCGATCGCGGGCGGCCTCGTCGATCCGGCGCGCGTGCCGCGCGACGGCGTCGCGACCG
TGCGGATCTGGCAGGCGAACATCGGCAAGACGATCGTCGCGCACGTGCCGATCACGAACGGCGCGGTGCAGGAGACGGGC
GATTTCGAGCTGGACGGCGTGACGTTCCCGGCCGCCGAAGTGCAGCTCGAGTTCATGGATCCGGCCGCCGACGAAGAGGG
CGCGGGCGGCGCGATGTTCCCGACCGGCAACCTCGTCGACGATCTGACGGTGCCGGGCGTCGGCACGCTGAAGGCGACGA
TGATCAACGCGGGGATTCCGACGATCTTCGTCGACGCGGAAGCGATCGGCTACAAGGGCACCGAGCTGCAGGACGCGATC
AACGGCGACGCGAAGGCGCTCGAGAAGTTCGAGACGATCCGCGCGCACGGCGCGCTGCGCATGGGGCTGATCGACACGCT
CGACGAGATCGCGACGCGGCAGCATACGCCGAAGATCGCGTTCGTCGCCAAGCCGGCCGACTATGTCGCGTCGAGCGGCA
AGCGCGTGAACGCGGGCGACGTCGACCTGCTGGTGCGCGCGATGTCGATGGGCAAGCTGCATCACGCGATGATGGGCACG
GCGGCCGTCGCGATCGGCACGGCCGCGGCGATTCCCGGCACGCTCGTCAATCTCGCGGCCGGCGGCGGCGAGCGCAACGC
GGTGCGCTTCGGGCATCCGTCCGGCACGCTGCGCGTCGGCGCCGAGGCGAAGTTCGAGAACGGCGAATGGACGGTCACGA
AGGCGATCATGAGCCGCAGTGCGCGCGTGCTGATGGAAGGGTGGGTGCGCGTGCCGGGCGACGCGTTCTGA

Upstream 100 bases:

>100_bases
TCGATCTACGAGGCGGGCGGCGTGCTGCAGCGCTTCGCGCAGGACTTCCTCGAATCGTCGCAGGCCGCGGCCTGATCCGA
CCACACTGGAGCAACGCAAC

Downstream 100 bases:

>100_bases
CGCGACCGGCCATCGATTCGATCCCCGTGAAGCGGAGTCGCTGCGCGGGGATTTTTTGTTTGAGCGGCGTGCGAGCGCGA
ATGTCGATCGACCGCGACGC

Product: probable AcnD-accessory protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAHIPQIRIPATYMRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSTRP
GHDVDYLFGQVAIDRAFVDWTGNCGNLSAAVGPFAIAGGLVDPARVPRDGVATVRIWQANIGKTIVAHVPITNGAVQETG
DFELDGVTFPAAEVQLEFMDPAADEEGAGGAMFPTGNLVDDLTVPGVGTLKATMINAGIPTIFVDAEAIGYKGTELQDAI
NGDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGDVDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVGAEAKFENGEWTVTKAIMSRSARVLMEGWVRVPGDAF

Sequences:

>Translated_396_residues
MAHIPQIRIPATYMRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSTRP
GHDVDYLFGQVAIDRAFVDWTGNCGNLSAAVGPFAIAGGLVDPARVPRDGVATVRIWQANIGKTIVAHVPITNGAVQETG
DFELDGVTFPAAEVQLEFMDPAADEEGAGGAMFPTGNLVDDLTVPGVGTLKATMINAGIPTIFVDAEAIGYKGTELQDAI
NGDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGDVDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVGAEAKFENGEWTVTKAIMSRSARVLMEGWVRVPGDAF
>Mature_395_residues
AHIPQIRIPATYMRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKTVIVSKSTRPG
HDVDYLFGQVAIDRAFVDWTGNCGNLSAAVGPFAIAGGLVDPARVPRDGVATVRIWQANIGKTIVAHVPITNGAVQETGD
FELDGVTFPAAEVQLEFMDPAADEEGAGGAMFPTGNLVDDLTVPGVGTLKATMINAGIPTIFVDAEAIGYKGTELQDAIN
GDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGDVDLLVRAMSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVGAEAKFENGEWTVTKAIMSRSARVLMEGWVRVPGDAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=386, Percent_Identity=33.9378238341969, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41285; Mature: 41153

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIPQIRIPATYMRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDG
CCCCCCEECCHHHHCCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCC
MGGATSSTSKTVIVSKSTRPGHDVDYLFGQVAIDRAFVDWTGNCGNLSAAVGPFAIAGGL
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCHHHCCHHHHCCC
VDPARVPRDGVATVRIWQANIGKTIVAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD
CCHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEEGAGGAMFPTGNLVDDLTVPGVGTLKATMINAGIPTIFVDAEAIGYKGTELQDAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECHHCCCCCCCHHHHH
NGDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGD
CCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCCC
VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEEC
AEAKFENGEWTVTKAIMSRSARVLMEGWVRVPGDAF
CEEEECCCCEEEEHHHHHHHHHEEHHCCEECCCCCC
>Mature Secondary Structure 
AHIPQIRIPATYMRGGTSKGVFFRLQDLPDAAQAPGAARDALLLRVIGSPDPYGKQIDG
CCCCCEECCHHHHCCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCC
MGGATSSTSKTVIVSKSTRPGHDVDYLFGQVAIDRAFVDWTGNCGNLSAAVGPFAIAGGL
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHEEEEEEEECCCCCCCCHHHCCHHHHCCC
VDPARVPRDGVATVRIWQANIGKTIVAHVPITNGAVQETGDFELDGVTFPAAEVQLEFMD
CCHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEEGAGGAMFPTGNLVDDLTVPGVGTLKATMINAGIPTIFVDAEAIGYKGTELQDAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECHHCCCCCCCHHHHH
NGDAKALEKFETIRAHGALRMGLIDTLDEIATRQHTPKIAFVAKPADYVASSGKRVNAGD
CCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCCC
VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEEC
AEAKFENGEWTVTKAIMSRSARVLMEGWVRVPGDAF
CEEEECCCCEEEEHHHHHHHHHEEHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA