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The map label for this gene is mrcA [H]

Identifier: 189353941

GI number: 189353941

Start: 2257210

End: 2259354

Strand: Reverse

Name: mrcA [H]

Synonym: BMULJ_05194

Alternate gene names: 189353941

Gene position: 2259354-2257210 (Counterclockwise)

Preceding gene: 189353943

Following gene: 189353940

Centisome position: 91.35

GC content: 71.24

Gene sequence:

>2145_bases
ATGACCGACCGCACCGATTCCCGTCCCACGCCGCCCGCGCCGCCGCCTCGCCGCCGCGTGTGGCGCATGCTCGCGGGCGC
GCTGCTCGGCCTGACGCTCGCGGGTGCGGGCATCGCAGGATGGACGATTCATCGCATCTGGACGCAATTGCCGTCGGTCG
AGCAACTGGCCGTCTATCGTCCCGCGCTGCCGCTGCGGATCTTCTCGCGCGACGGCGAACTGCTTGCCGAATACGGCGTC
GAGCGCCGCGAGTTCGTGCCGCTCGAGCGCATGCCCGCGCGGATGCGCGACGCGCTGCTGGCGGCCGAGGACGCACAGTT
CTACCGGCACGGCGCGATCGACATCGGCGGCCTCGCGCGCGCGACGTTCGCGAACGTCGTCACCGGACAGCCGGCGCAAG
GCGGCAGCACGATCACGATGCAGGTCGCGCGCAACTTCTACCTGACGCGCGACAAGGTGCTGAGCCGCAAGCTCGCCGAG
ATCCTGATGGCGATCAAGCTCGAGCGCGCGTACGGCAAGGACAAGCTGCTCGAGCTGTACATGAACGAGATCTATCTCGG
CGAGCCCGCGTACGGGTTCGCGGCAGCGGCGCACGTGTATTTCGGCAAGCCGCTCGATGCGTTGAGCGTCGGCGAAGCGG
CTGTGCTTGCCGGGTTGCCGAAGGCGCCGTCCGCGTTCAATCCGGTCGTCAATCCGGCGCGCGCGACCGCGCGTCGCAAC
TACGTGCTCGGCCGCATGCTTGCGCTCGGCCAACTCGACGACGCGACGTACCGCGCGGCCGTCGATGCGCCGATCGCGCT
GTCGCCGCAGGCGCCGCCGGGCATCGTCGCGGCGCCGTACGTCGCCGAACGCGCGCGGCTGATGATGGTCGAGCGCTTTC
GCGACGACGCGTATACGCTCGGCCTCGACGTGACGACGACGATCTCGATGCGCGACCAGCGCGCGGCCGAGGCGGCGCTC
GAGCGCACGCTGCGCCGACAGCGGACGACGCGCGACGCGCAGCGCACGCTCGAGGGTGCGCTCGTGTCGCTCGATGCGGC
GACCGGCGACATGCTCGCGCTCGTCGGCGGCGCGGATTTCAGTTGCAACGTGTTCGACCATGCGTTGCAGGCCTACCGTC
AGCCGGGGTCGAGCTTCAAGCCGTTCGTCTATTCGGCGGCGCTTGAGAAAGGCTATTTCCCCGGCGTGCTCGTCGACGAT
ACGCAGCGCACGCTGACGCCGGCCGAGACCGGCGCACGGCCGTGGCGGCCGCGCAATTACGGCAACCATTACGAGGGCTT
CATCGCGGTGCGGCGCGGGCTCGTGCGATCGAAGAACCTCGTCGCGGTGAGCCTGATGCAGGCGGCCGACCCGCAATACG
TGCAGCAGCACGCGATGCGCTTCGGTTTCGATGCGCGGCGCAATCCGGCGTCGCTGCCGCTCGCGCTCGGCGCGGGCGCG
GTGACGCCGCTCGAACTCGCGAGCGCGTACGGCGTGTTCGCGAATGGCGGCACGCGAGTGGAACCGCGGCTGATCCTCTC
GGTGAAGCAGCGGCACGGCGGCGTGCTGTACGACGCGCCGGCGCCGTCCGGCACGCGCGTCGTGTCGGCGCGCAACGCAT
TCGTGATGGACAGCATGCTGCGCGACGTCGTGCGTGCGGGCACCGCGCGCGGCGCGCTTGCGCTGCGCCGCGACGACACG
GCCGGCAAGACCGGCACGTCGAACGGCTCGAAGGACGTGTGGTTCGCCGGCTATTCGTCGGGCGTCGTCGCGGTCGCGTG
GCTCGGCTACGACACGCCGCGCTCGATGGGGCGCGCGACCGGCGCGACGCTCGCGCTGCCCGTGTGGCTCGACTACATGA
AGACGGCCGTCGACGGACGCACGCCGGCCGAAGCGGTCGAGCCGCCGGACGTCGCGCGTGTCGACGGCGATTTCGTCTAT
GCCGAATACACGCGCGGCGCGTGCACGGCCGATGTGCCGGCGTTCGTACGCAGTCGCTTCGCATGCGACGGCGCCGCCCG
CGCGGAGGCGGCATCCGCCGGGTTCGCCGCGTCCGCCGCGTCCACTGCGGCGAACGGCGGTGCGATGCCGGTCGCGCCGA
CGCCGGCACCGGTCGATGCGGCCGAGCGCGCACGCGTGCTCGACCTGTTCCGGACCGAAGACTGA

Upstream 100 bases:

>100_bases
GAATGTTCCGGTTTCACGGCATCCCTTCGACAATGCGCGCCGTTCATGATCGTCGGCCGCGTGGTGCGCCGTCGATCGCC
GCATTGCCGAAGGAGCCGAC

Downstream 100 bases:

>100_bases
CGCGAACGACGATGCACACGCTTTATCTGATCGCGATCGTCGCGGAGGCGATGTCGGGCGCGCTGATGGGCATGCGGCGC
GGGATGGACCGCTTCGGGCT

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MTDRTDSRPTPPAPPPRRRVWRMLAGALLGLTLAGAGIAGWTIHRIWTQLPSVEQLAVYRPALPLRIFSRDGELLAEYGV
ERREFVPLERMPARMRDALLAAEDAQFYRHGAIDIGGLARATFANVVTGQPAQGGSTITMQVARNFYLTRDKVLSRKLAE
ILMAIKLERAYGKDKLLELYMNEIYLGEPAYGFAAAAHVYFGKPLDALSVGEAAVLAGLPKAPSAFNPVVNPARATARRN
YVLGRMLALGQLDDATYRAAVDAPIALSPQAPPGIVAAPYVAERARLMMVERFRDDAYTLGLDVTTTISMRDQRAAEAAL
ERTLRRQRTTRDAQRTLEGALVSLDAATGDMLALVGGADFSCNVFDHALQAYRQPGSSFKPFVYSAALEKGYFPGVLVDD
TQRTLTPAETGARPWRPRNYGNHYEGFIAVRRGLVRSKNLVAVSLMQAADPQYVQQHAMRFGFDARRNPASLPLALGAGA
VTPLELASAYGVFANGGTRVEPRLILSVKQRHGGVLYDAPAPSGTRVVSARNAFVMDSMLRDVVRAGTARGALALRRDDT
AGKTGTSNGSKDVWFAGYSSGVVAVAWLGYDTPRSMGRATGATLALPVWLDYMKTAVDGRTPAEAVEPPDVARVDGDFVY
AEYTRGACTADVPAFVRSRFACDGAARAEAASAGFAASAASTAANGGAMPVAPTPAPVDAAERARVLDLFRTED

Sequences:

>Translated_714_residues
MTDRTDSRPTPPAPPPRRRVWRMLAGALLGLTLAGAGIAGWTIHRIWTQLPSVEQLAVYRPALPLRIFSRDGELLAEYGV
ERREFVPLERMPARMRDALLAAEDAQFYRHGAIDIGGLARATFANVVTGQPAQGGSTITMQVARNFYLTRDKVLSRKLAE
ILMAIKLERAYGKDKLLELYMNEIYLGEPAYGFAAAAHVYFGKPLDALSVGEAAVLAGLPKAPSAFNPVVNPARATARRN
YVLGRMLALGQLDDATYRAAVDAPIALSPQAPPGIVAAPYVAERARLMMVERFRDDAYTLGLDVTTTISMRDQRAAEAAL
ERTLRRQRTTRDAQRTLEGALVSLDAATGDMLALVGGADFSCNVFDHALQAYRQPGSSFKPFVYSAALEKGYFPGVLVDD
TQRTLTPAETGARPWRPRNYGNHYEGFIAVRRGLVRSKNLVAVSLMQAADPQYVQQHAMRFGFDARRNPASLPLALGAGA
VTPLELASAYGVFANGGTRVEPRLILSVKQRHGGVLYDAPAPSGTRVVSARNAFVMDSMLRDVVRAGTARGALALRRDDT
AGKTGTSNGSKDVWFAGYSSGVVAVAWLGYDTPRSMGRATGATLALPVWLDYMKTAVDGRTPAEAVEPPDVARVDGDFVY
AEYTRGACTADVPAFVRSRFACDGAARAEAASAGFAASAASTAANGGAMPVAPTPAPVDAAERARVLDLFRTED
>Mature_713_residues
TDRTDSRPTPPAPPPRRRVWRMLAGALLGLTLAGAGIAGWTIHRIWTQLPSVEQLAVYRPALPLRIFSRDGELLAEYGVE
RREFVPLERMPARMRDALLAAEDAQFYRHGAIDIGGLARATFANVVTGQPAQGGSTITMQVARNFYLTRDKVLSRKLAEI
LMAIKLERAYGKDKLLELYMNEIYLGEPAYGFAAAAHVYFGKPLDALSVGEAAVLAGLPKAPSAFNPVVNPARATARRNY
VLGRMLALGQLDDATYRAAVDAPIALSPQAPPGIVAAPYVAERARLMMVERFRDDAYTLGLDVTTTISMRDQRAAEAALE
RTLRRQRTTRDAQRTLEGALVSLDAATGDMLALVGGADFSCNVFDHALQAYRQPGSSFKPFVYSAALEKGYFPGVLVDDT
QRTLTPAETGARPWRPRNYGNHYEGFIAVRRGLVRSKNLVAVSLMQAADPQYVQQHAMRFGFDARRNPASLPLALGAGAV
TPLELASAYGVFANGGTRVEPRLILSVKQRHGGVLYDAPAPSGTRVVSARNAFVMDSMLRDVVRAGTARGALALRRDDTA
GKTGTSNGSKDVWFAGYSSGVVAVAWLGYDTPRSMGRATGATLALPVWLDYMKTAVDGRTPAEAVEPPDVARVDGDFVYA
EYTRGACTADVPAFVRSRFACDGAARAEAASAGFAASAASTAANGGAMPVAPTPAPVDAAERARVLDLFRTED

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=577, Percent_Identity=33.4488734835355, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87082258, Length=292, Percent_Identity=43.8356164383562, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1788867, Length=593, Percent_Identity=30.185497470489, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789601, Length=171, Percent_Identity=29.8245614035088, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 76755; Mature: 76624

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRTDSRPTPPAPPPRRRVWRMLAGALLGLTLAGAGIAGWTIHRIWTQLPSVEQLAVYR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHC
PALPLRIFSRDGELLAEYGVERREFVPLERMPARMRDALLAAEDAQFYRHGAIDIGGLAR
CCCCEEEECCCCHHHHHCCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ATFANVVTGQPAQGGSTITMQVARNFYLTRDKVLSRKLAEILMAIKLERAYGKDKLLELY
HHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
MNEIYLGEPAYGFAAAAHVYFGKPLDALSVGEAAVLAGLPKAPSAFNPVVNPARATARRN
HHHHEECCCCHHHHHHHHHHCCCCCCHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHH
YVLGRMLALGQLDDATYRAAVDAPIALSPQAPPGIVAAPYVAERARLMMVERFRDDAYTL
HHHHHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEE
GLDVTTTISMRDQRAAEAALERTLRRQRTTRDAQRTLEGALVSLDAATGDMLALVGGADF
ECEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCC
SCNVFDHALQAYRQPGSSFKPFVYSAALEKGYFPGVLVDDTQRTLTPAETGARPWRPRNY
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCHHCCCCCCCCCCC
GNHYEGFIAVRRGLVRSKNLVAVSLMQAADPQYVQQHAMRFGFDARRNPASLPLALGAGA
CCCHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
VTPLELASAYGVFANGGTRVEPRLILSVKQRHGGVLYDAPAPSGTRVVSARNAFVMDSML
CCHHHHHHHCCEEECCCCCCCCEEEEEEHHHCCCEEEECCCCCCCEEEECCCHHHHHHHH
RDVVRAGTARGALALRRDDTAGKTGTSNGSKDVWFAGYSSGVVAVAWLGYDTPRSMGRAT
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHCC
GATLALPVWLDYMKTAVDGRTPAEAVEPPDVARVDGDFVYAEYTRGACTADVPAFVRSRF
CCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHHH
ACDGAARAEAASAGFAASAASTAANGGAMPVAPTPAPVDAAERARVLDLFRTED
CCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDRTDSRPTPPAPPPRRRVWRMLAGALLGLTLAGAGIAGWTIHRIWTQLPSVEQLAVYR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHC
PALPLRIFSRDGELLAEYGVERREFVPLERMPARMRDALLAAEDAQFYRHGAIDIGGLAR
CCCCEEEECCCCHHHHHCCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ATFANVVTGQPAQGGSTITMQVARNFYLTRDKVLSRKLAEILMAIKLERAYGKDKLLELY
HHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
MNEIYLGEPAYGFAAAAHVYFGKPLDALSVGEAAVLAGLPKAPSAFNPVVNPARATARRN
HHHHEECCCCHHHHHHHHHHCCCCCCHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHH
YVLGRMLALGQLDDATYRAAVDAPIALSPQAPPGIVAAPYVAERARLMMVERFRDDAYTL
HHHHHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEE
GLDVTTTISMRDQRAAEAALERTLRRQRTTRDAQRTLEGALVSLDAATGDMLALVGGADF
ECEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCC
SCNVFDHALQAYRQPGSSFKPFVYSAALEKGYFPGVLVDDTQRTLTPAETGARPWRPRNY
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEECCCCCCCCCHHCCCCCCCCCCC
GNHYEGFIAVRRGLVRSKNLVAVSLMQAADPQYVQQHAMRFGFDARRNPASLPLALGAGA
CCCHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
VTPLELASAYGVFANGGTRVEPRLILSVKQRHGGVLYDAPAPSGTRVVSARNAFVMDSML
CCHHHHHHHCCEEECCCCCCCCEEEEEEHHHCCCEEEECCCCCCCEEEECCCHHHHHHHH
RDVVRAGTARGALALRRDDTAGKTGTSNGSKDVWFAGYSSGVVAVAWLGYDTPRSMGRAT
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHCC
GATLALPVWLDYMKTAVDGRTPAEAVEPPDVARVDGDFVYAEYTRGACTADVPAFVRSRF
CCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHHH
ACDGAARAEAASAGFAASAASTAANGGAMPVAPTPAPVDAAERARVLDLFRTED
CCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]