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The map label for this gene is aes [H]

Identifier: 189353680

GI number: 189353680

Start: 1942805

End: 1943785

Strand: Reverse

Name: aes [H]

Synonym: BMULJ_04925

Alternate gene names: 189353680

Gene position: 1943785-1942805 (Counterclockwise)

Preceding gene: 189353681

Following gene: 189353677

Centisome position: 78.6

GC content: 74.11

Gene sequence:

>981_bases
GTGCTGGAACCGGAAATCGCGGCGTTCGTCGCGGCGGTCGATGCGTGGTATCCACCGGGCGCGGCCGCGTGCGCGCCCGA
CGAGCAGCGTCGCCTGTACGACCGCTTCGCGGCCGCGTGGACGCCCGCGACGCTGCCGGCCGGCATCGCACGGCAGGACG
CGGTATGGCAGGCGCCGCACGGCCAGCCGATCGCGCTGCGGCGCTACGCGCCGACGCACGGTGCGCCGCGCGGCACGGTG
CTGTTTTTTCATGGCGGCGGGTTCGTCGTCGGCTCGCTCGACAGTCATCAGCTGATCACCGCGCAGCTCGCGGCCGATAC
GGGCCTCGCCGTGATCGCGGTCGATTACCGGCTCGCGCCCGAGCATCGAGCGCCTGCCGCGCTCGAGGATTGCGCGGCCG
TCGCGCGCGCGGCACGCGATGCGCGCCTGCCGTTCGGCCCGTGCGCGGCGCCGCTGACGCTCGCCGGCGACAGCGCGGGC
GGCAATCTCGCGGCGGCCGTCGCGACCGTGCTGCGCGATGCGGACGCGCGCGGCGTCGAGGGCATCGACGGCGTTGCGCT
CGTCTATCCGATGCTCGGCTTCGAACCGCAATCGCCGGCGCGCGAGACGGAAGCGCATGCGCCGATGCTGACGCTCGACG
ACGTGCATCGCTATCGCGCGTGGTACTGGGGCGAAGGCGAGGCAACGGCGCGCAGCGGCGATCGTGCGTCGCACGCCGAT
CCGCTGCTCCGCGCATCGCTGCCGCTGGAGGCCGCGCGTTTCGACGGACTGCCGCCGGTGCTCGCGATCGGTGCCGAGCA
CGATCCGCTGCGCGACGATGCGCGCGTGTACGTCGAACGGATTCGCGCGGCCGGCGGCCGTGCGCGCTACTGGATGGGCG
AGGGGCTCGTGCACGGCTGCTGGCGCGCACTCGCGACGAGTCCGCAGGCCGCGCGCCTGCACCGCATGGTCGGCGCGTTC
CTGCTCGCGCCCGATACCTAG

Upstream 100 bases:

>100_bases
TGGCCGAGCTTCACGCGTTGCAGCGCGATTGCGCGGCGCCCGCGTATGCGGGCCGCGTGATCGCGCTGCCGCCGCGCCGC
TAGCGTACGCGGGCCGCGCG

Downstream 100 bases:

>100_bases
CCGGCCTGCGCGCGCAGTGCGCGTACCGCCGACGAAAAAGCGCCCGGACGCGATGCGCCCGGGCGAAGCCACCCGTTGCC
GGGCGGCGGAGGTATGCGGT

Product: putative esterase/lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLEPEIAAFVAAVDAWYPPGAAACAPDEQRRLYDRFAAAWTPATLPAGIARQDAVWQAPHGQPIALRRYAPTHGAPRGTV
LFFHGGGFVVGSLDSHQLITAQLAADTGLAVIAVDYRLAPEHRAPAALEDCAAVARAARDARLPFGPCAAPLTLAGDSAG
GNLAAAVATVLRDADARGVEGIDGVALVYPMLGFEPQSPARETEAHAPMLTLDDVHRYRAWYWGEGEATARSGDRASHAD
PLLRASLPLEAARFDGLPPVLAIGAEHDPLRDDARVYVERIRAAGGRARYWMGEGLVHGCWRALATSPQAARLHRMVGAF
LLAPDT

Sequences:

>Translated_326_residues
MLEPEIAAFVAAVDAWYPPGAAACAPDEQRRLYDRFAAAWTPATLPAGIARQDAVWQAPHGQPIALRRYAPTHGAPRGTV
LFFHGGGFVVGSLDSHQLITAQLAADTGLAVIAVDYRLAPEHRAPAALEDCAAVARAARDARLPFGPCAAPLTLAGDSAG
GNLAAAVATVLRDADARGVEGIDGVALVYPMLGFEPQSPARETEAHAPMLTLDDVHRYRAWYWGEGEATARSGDRASHAD
PLLRASLPLEAARFDGLPPVLAIGAEHDPLRDDARVYVERIRAAGGRARYWMGEGLVHGCWRALATSPQAARLHRMVGAF
LLAPDT
>Mature_326_residues
MLEPEIAAFVAAVDAWYPPGAAACAPDEQRRLYDRFAAAWTPATLPAGIARQDAVWQAPHGQPIALRRYAPTHGAPRGTV
LFFHGGGFVVGSLDSHQLITAQLAADTGLAVIAVDYRLAPEHRAPAALEDCAAVARAARDARLPFGPCAAPLTLAGDSAG
GNLAAAVATVLRDADARGVEGIDGVALVYPMLGFEPQSPARETEAHAPMLTLDDVHRYRAWYWGEGEATARSGDRASHAD
PLLRASLPLEAARFDGLPPVLAIGAEHDPLRDDARVYVERIRAAGGRARYWMGEGLVHGCWRALATSPQAARLHRMVGAF
LLAPDT

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=299, Percent_Identity=28.4280936454849, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI157041239, Length=292, Percent_Identity=23.972602739726, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI21328446, Length=150, Percent_Identity=39.3333333333333, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI61966717, Length=285, Percent_Identity=24.5614035087719, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786682, Length=322, Percent_Identity=29.1925465838509, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17540028, Length=290, Percent_Identity=25.1724137931034, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=355, Percent_Identity=25.3521126760563, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=141, Percent_Identity=31.2056737588652, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17567059, Length=251, Percent_Identity=28.6852589641434, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34589; Mature: 34589

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPEIAAFVAAVDAWYPPGAAACAPDEQRRLYDRFAAAWTPATLPAGIARQDAVWQAPH
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
GQPIALRRYAPTHGAPRGTVLFFHGGGFVVGSLDSHQLITAQLAADTGLAVIAVDYRLAP
CCCEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEEECCCCCEEEEEEEEEECC
EHRAPAALEDCAAVARAARDARLPFGPCAAPLTLAGDSAGGNLAAAVATVLRDADARGVE
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCC
GIDGVALVYPMLGFEPQSPARETEAHAPMLTLDDVHRYRAWYWGEGEATARSGDRASHAD
CCCCHHHHHHHHCCCCCCCCHHHHHCCCEEEHHHHHHHHEEEECCCCCCCCCCCCCCCCC
PLLRASLPLEAARFDGLPPVLAIGAEHDPLRDDARVYVERIRAAGGRARYWMGEGLVHGC
CHHHHCCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHH
WRALATSPQAARLHRMVGAFLLAPDT
HHHHHCCCHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MLEPEIAAFVAAVDAWYPPGAAACAPDEQRRLYDRFAAAWTPATLPAGIARQDAVWQAPH
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
GQPIALRRYAPTHGAPRGTVLFFHGGGFVVGSLDSHQLITAQLAADTGLAVIAVDYRLAP
CCCEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEEECCCCCEEEEEEEEEECC
EHRAPAALEDCAAVARAARDARLPFGPCAAPLTLAGDSAGGNLAAAVATVLRDADARGVE
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCC
GIDGVALVYPMLGFEPQSPARETEAHAPMLTLDDVHRYRAWYWGEGEATARSGDRASHAD
CCCCHHHHHHHHCCCCCCCCHHHHHCCCEEEHHHHHHHHEEEECCCCCCCCCCCCCCCCC
PLLRASLPLEAARFDGLPPVLAIGAEHDPLRDDARVYVERIRAAGGRARYWMGEGLVHGC
CHHHHCCCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHH
WRALATSPQAARLHRMVGAFLLAPDT
HHHHHCCCHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA