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The map label for this gene is yfiN [H]

Identifier: 189353655

GI number: 189353655

Start: 1915001

End: 1916269

Strand: Reverse

Name: yfiN [H]

Synonym: BMULJ_04900

Alternate gene names: 189353655

Gene position: 1916269-1915001 (Counterclockwise)

Preceding gene: 189353656

Following gene: 189353654

Centisome position: 77.48

GC content: 69.03

Gene sequence:

>1269_bases
ATGACCCGATCCGCGCCGCCCGTTTCGTCTTCGCGCGTGCCGCGCCCGACGCTGCAAAGCGTGTTGCGACGCGCGCATCT
GCGACTCGCGTTCGTCGCCGTCGCGATGGCCGCCGTGTCATTGATCGTCGTCGCCGTGATCGCATTGCGCGCGTATGCCG
GCAACAACCTGAACCTGCTCGCGCGCTCGCTGGCGTATACCGTCGAGGCCGCGCTCGTGTTTCGCGACCGCGTCGCGGCC
GCGGAGGCGATCGCGCTGATTGCTGCGGACGAGGACGTGGCGCAGGTCGTCGTCACCGATACGGACGGGCGGCCATTCGC
AACGTGGCAGTTGCGTGCGCCTAGCCGGCTCGCACGCGTCGAACGCGCGGTTGCGGATTTCGCGCTGCCGGGGCCCGCGA
GCGTAGCGGTGCTGCACGACGGCATCGTCGTCGGACATGTCGTCGTGCGCGGGCGCGGACAACAGTTCTTCGGTTTCCTG
CTCGGCGGCATCGGCGGCATTCTGGGCTGCCTGATCGTCAGCGTGCTCGGTGCTTACGTCAGTTCCAAGCGGCTGATGCG
CAACATCGTCGCGCCGCTGCGCGCGCTGGCCGACGTCGCGCATGCCGTGCGACGCGAGCGTGCATTCGGGCGACGGGTCG
AGCCGGTCGCGATCGACGAGCTCAATCAGCTCGGCGCGGACTTCAATGCGTTGCTCGACGAATTCGAACAGTGGCAGGAC
ACGCTGCGTCACGAGAACGCATCGCTCGCACACAAGGCGATGCACGATGCGCTCACCGGGCTGCCGAATCGCGTGCAGTT
CGAGTCGCGGCTCGCGCGCGCGCTCGGCGAGGCAGGCGCGACCGGGCAGCGTGTCGCGATTCTGTATCTCGACTGCGACG
GCTTCAAGGAGATCAACGACTGCTTCGGTCATGAGGCGGGCGACGCGGTGCTGATCGGTATCGCGGCGCGGCTGCGCGCG
CAGCTGCGCGATGCCGATCTGATCGCGCGGCTCGGCGGCGACGAGTTCGCGGTGATGCTCGAGACCGTGCGCGAGGCGGG
CAGCGTCGGCCGGATCGCGGATGCGATCCTGTCCGGCATGGCGCCGTCGATCGAGCTGTCCGACGGGCGCATGGTCGCGA
CGATGATGAGCGTCGGGATCGCGCTGTATCCCGATCATGCGTCGGATGCGACCGGTTTGCTGCGCGCGGCGGACGCCGCG
ATGTATCGCGCGAAGCGCACGCGACCGGGATCGTGGCAGTCGGCGGGCGGAGCGGCGGGATCGGCGTGA

Upstream 100 bases:

>100_bases
GCGAACCCGTCACGTTCGACATCAATCTCGATGCGGTGGCGCGCAGCGGCGTGCGCGTGCATCCGAACGTGCTCAATCTC
GCGCGGCGGCCGGTGACGCC

Downstream 100 bases:

>100_bases
TGTCGATGGCGTCGCGCGCATATTCGTGTGGAAAGGGGGCGATGATGGGACACTCGTTGCCTGTGCGCCGGGGGCGCATG
TGGGTTGGCGGCGCGACGCT

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTRSAPPVSSSRVPRPTLQSVLRRAHLRLAFVAVAMAAVSLIVVAVIALRAYAGNNLNLLARSLAYTVEAALVFRDRVAA
AEAIALIAADEDVAQVVVTDTDGRPFATWQLRAPSRLARVERAVADFALPGPASVAVLHDGIVVGHVVVRGRGQQFFGFL
LGGIGGILGCLIVSVLGAYVSSKRLMRNIVAPLRALADVAHAVRRERAFGRRVEPVAIDELNQLGADFNALLDEFEQWQD
TLRHENASLAHKAMHDALTGLPNRVQFESRLARALGEAGATGQRVAILYLDCDGFKEINDCFGHEAGDAVLIGIAARLRA
QLRDADLIARLGGDEFAVMLETVREAGSVGRIADAILSGMAPSIELSDGRMVATMMSVGIALYPDHASDATGLLRAADAA
MYRAKRTRPGSWQSAGGAAGSA

Sequences:

>Translated_422_residues
MTRSAPPVSSSRVPRPTLQSVLRRAHLRLAFVAVAMAAVSLIVVAVIALRAYAGNNLNLLARSLAYTVEAALVFRDRVAA
AEAIALIAADEDVAQVVVTDTDGRPFATWQLRAPSRLARVERAVADFALPGPASVAVLHDGIVVGHVVVRGRGQQFFGFL
LGGIGGILGCLIVSVLGAYVSSKRLMRNIVAPLRALADVAHAVRRERAFGRRVEPVAIDELNQLGADFNALLDEFEQWQD
TLRHENASLAHKAMHDALTGLPNRVQFESRLARALGEAGATGQRVAILYLDCDGFKEINDCFGHEAGDAVLIGIAARLRA
QLRDADLIARLGGDEFAVMLETVREAGSVGRIADAILSGMAPSIELSDGRMVATMMSVGIALYPDHASDATGLLRAADAA
MYRAKRTRPGSWQSAGGAAGSA
>Mature_421_residues
TRSAPPVSSSRVPRPTLQSVLRRAHLRLAFVAVAMAAVSLIVVAVIALRAYAGNNLNLLARSLAYTVEAALVFRDRVAAA
EAIALIAADEDVAQVVVTDTDGRPFATWQLRAPSRLARVERAVADFALPGPASVAVLHDGIVVGHVVVRGRGQQFFGFLL
GGIGGILGCLIVSVLGAYVSSKRLMRNIVAPLRALADVAHAVRRERAFGRRVEPVAIDELNQLGADFNALLDEFEQWQDT
LRHENASLAHKAMHDALTGLPNRVQFESRLARALGEAGATGQRVAILYLDCDGFKEINDCFGHEAGDAVLIGIAARLRAQ
LRDADLIARLGGDEFAVMLETVREAGSVGRIADAILSGMAPSIELSDGRMVATMMSVGIALYPDHASDATGLLRAADAAM
YRAKRTRPGSWQSAGGAAGSA

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=401, Percent_Identity=34.1645885286783, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=39.2405063291139, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081977, Length=210, Percent_Identity=32.3809523809524, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=37.3417721518987, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=33.1360946745562, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1786584, Length=157, Percent_Identity=31.2101910828025, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=34.9693251533742, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=30.9090909090909, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082007, Length=96, Percent_Identity=44.7916666666667, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787802, Length=163, Percent_Identity=31.9018404907975, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=28.2208588957055, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=31.6129032258064, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44749; Mature: 44618

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSAPPVSSSRVPRPTLQSVLRRAHLRLAFVAVAMAAVSLIVVAVIALRAYAGNNLNLL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ARSLAYTVEAALVFRDRVAAAEAIALIAADEDVAQVVVTDTDGRPFATWQLRAPSRLARV
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCHHHHHHH
ERAVADFALPGPASVAVLHDGIVVGHVVVRGRGQQFFGFLLGGIGGILGCLIVSVLGAYV
HHHHHHHCCCCCCEEEEEECCEEEEHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSKRLMRNIVAPLRALADVAHAVRRERAFGRRVEPVAIDELNQLGADFNALLDEFEQWQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
TLRHENASLAHKAMHDALTGLPNRVQFESRLARALGEAGATGQRVAILYLDCDGFKEIND
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHH
CFGHEAGDAVLIGIAARLRAQLRDADLIARLGGDEFAVMLETVREAGSVGRIADAILSGM
HCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
APSIELSDGRMVATMMSVGIALYPDHASDATGLLRAADAAMYRAKRTRPGSWQSAGGAAG
CCCEEECCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SA
CC
>Mature Secondary Structure 
TRSAPPVSSSRVPRPTLQSVLRRAHLRLAFVAVAMAAVSLIVVAVIALRAYAGNNLNLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ARSLAYTVEAALVFRDRVAAAEAIALIAADEDVAQVVVTDTDGRPFATWQLRAPSRLARV
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCHHHHHHH
ERAVADFALPGPASVAVLHDGIVVGHVVVRGRGQQFFGFLLGGIGGILGCLIVSVLGAYV
HHHHHHHCCCCCCEEEEEECCEEEEHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSKRLMRNIVAPLRALADVAHAVRRERAFGRRVEPVAIDELNQLGADFNALLDEFEQWQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
TLRHENASLAHKAMHDALTGLPNRVQFESRLARALGEAGATGQRVAILYLDCDGFKEIND
HHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHH
CFGHEAGDAVLIGIAARLRAQLRDADLIARLGGDEFAVMLETVREAGSVGRIADAILSGM
HCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
APSIELSDGRMVATMMSVGIALYPDHASDATGLLRAADAAMYRAKRTRPGSWQSAGGAAG
CCCEEECCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]