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The map label for this gene is livK [H]

Identifier: 189353649

GI number: 189353649

Start: 1908825

End: 1909943

Strand: Reverse

Name: livK [H]

Synonym: BMULJ_04894

Alternate gene names: 189353649

Gene position: 1909943-1908825 (Counterclockwise)

Preceding gene: 189353652

Following gene: 189353648

Centisome position: 77.23

GC content: 64.16

Gene sequence:

>1119_bases
ATGACGCTGTCCCGTCTTACGTCCATTTCCGTCGCCGCCGTGCTGTTCGCCGCCGGCGCCGCTGCCGCGCAAGCGGAAAC
CGTGAAGATCGCCATCGCCGGCCCGATGAGCGGCTCGGTCGCCCAGTATGGCGACATGGTCAAGGCCGGCGCGCTGACCG
CGATCGAGCAGATCAACGCGGCAGGCGGTGCGGGCGGCAACAAGTTCGAAGTGGTGATGATGGACGACGCCTGCGAACCG
AAGCAGGCGGTCGCCGTCGCCAACAAGATCGTCAGCCAGAAAATCAAGTACGTGATCGGCCATGTGTGCTCGGGCTCGAC
GATTCCGGCATCCGACATCTACGAGAACGAAGGCATCGTGATGGTCACGCCGTCGGCCACCGCGCCGCAGCTGACCGAAG
GCAAGAAGCGTCACTTCATCTTCCGCACGATCGGCCGTGACGACCAGCAGGGTCCGGCCGCTGCGCAGTACATCATCAAT
CACGTGAAGCCGAAGAAGGTCGCGGTGCTGCACGACAAGCAGTCGTACGGCCAGGGCATCGCATCGTCGGTGAAGAAGGA
TCTCGAAGCGGCGAAGATTCCGGTCGTGCTGTTCGAAGGCATCAACGCCGGCGATTCGGATTACTCGGCGATCATCACGA
AGCTGAAGTCGCAAGGCGTCGACTTCGTCTACTTCGGCGGCTATCACCCGGAAATGGGCCTGCTGCTGCGCCAGGCGCGC
GAGCAGGGCGTGAAGGCGACGTTCATGGGGCCTGAGGGCGTCGGCAACAAGGACGTGACGGCCATCGCGGGCCCGGCATC
GGAAGGCATGCTCGTCACGCTGCCGGCCGACTTCTCGGCCGACCCGGCCAACGCGGCGCTCGTGAAGGCGTTCGCCGACA
AGAAGCGCGATCCGAACGGCCCGTTCCAGATGCCGGCATACGCGGCAGTGAAGATCATCGCCGACTCGATCGCCGGAGCG
AAGACGACCGACCCGACGAAGGTCGCCGCGTACATGCACAAGACGACGTTCGACACGCCGATCGGCAAGGTCGCGTATGA
CGCGCAGGGCGACCTGAAGGCGTTCAAGTTCGTCGTCTACACGTGGCACAAGGACGCGACGAAGACCGCCGCCAAGTAA

Upstream 100 bases:

>100_bases
GCGTCTCGGGCGTGTCCCATGTTCTCGCCCCGGGTGCTGTTCGATTGCGCAAGTCATGCCGCCTTTGCCCGTATGACTAA
ACAACATCGAGGAGCTCCTG

Downstream 100 bases:

>100_bases
CAACGGACTACCCGCCCGCGCACCCGCCCCGTCCGAGATCCGGACGGGGCGGGTGCGTATTGGTCGCCGTGCATCCGTGC
ATCGCGCGACCGCGGGCGAA

Product: branched-chain amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTLSRLTSISVAAVLFAAGAAAAQAETVKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGAGGNKFEVVMMDDACEP
KQAVAVANKIVSQKIKYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTEGKKRHFIFRTIGRDDQQGPAAAQYIIN
HVKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVVLFEGINAGDSDYSAIITKLKSQGVDFVYFGGYHPEMGLLLRQAR
EQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIADSIAGA
KTTDPTKVAAYMHKTTFDTPIGKVAYDAQGDLKAFKFVVYTWHKDATKTAAK

Sequences:

>Translated_372_residues
MTLSRLTSISVAAVLFAAGAAAAQAETVKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGAGGNKFEVVMMDDACEP
KQAVAVANKIVSQKIKYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTEGKKRHFIFRTIGRDDQQGPAAAQYIIN
HVKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVVLFEGINAGDSDYSAIITKLKSQGVDFVYFGGYHPEMGLLLRQAR
EQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIADSIAGA
KTTDPTKVAAYMHKTTFDTPIGKVAYDAQGDLKAFKFVVYTWHKDATKTAAK
>Mature_371_residues
TLSRLTSISVAAVLFAAGAAAAQAETVKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGAGGNKFEVVMMDDACEPK
QAVAVANKIVSQKIKYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTEGKKRHFIFRTIGRDDQQGPAAAQYIINH
VKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVVLFEGINAGDSDYSAIITKLKSQGVDFVYFGGYHPEMGLLLRQARE
QGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIADSIAGAK
TTDPTKVAAYMHKTTFDTPIGKVAYDAQGDLKAFKFVVYTWHKDATKTAAK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=370, Percent_Identity=50.8108108108108, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI48994941, Length=362, Percent_Identity=50.2762430939227, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39029; Mature: 38898

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSRLTSISVAAVLFAAGAAAAQAETVKIAIAGPMSGSVAQYGDMVKAGALTAIEQINA
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
AGGAGGNKFEVVMMDDACEPKQAVAVANKIVSQKIKYVIGHVCSGSTIPASDIYENEGIV
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEE
MVTPSATAPQLTEGKKRHFIFRTIGRDDQQGPAAAQYIINHVKPKKVAVLHDKQSYGQGI
EECCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHH
ASSVKKDLEAAKIPVVLFEGINAGDSDYSAIITKLKSQGVDFVYFGGYHPEMGLLLRQAR
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
EQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNG
HCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
PFQMPAYAAVKIIADSIAGAKTTDPTKVAAYMHKTTFDTPIGKVAYDAQGDLKAFKFVVY
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEEE
TWHKDATKTAAK
EECCCCHHHCCC
>Mature Secondary Structure 
TLSRLTSISVAAVLFAAGAAAAQAETVKIAIAGPMSGSVAQYGDMVKAGALTAIEQINA
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
AGGAGGNKFEVVMMDDACEPKQAVAVANKIVSQKIKYVIGHVCSGSTIPASDIYENEGIV
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEE
MVTPSATAPQLTEGKKRHFIFRTIGRDDQQGPAAAQYIINHVKPKKVAVLHDKQSYGQGI
EECCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHH
ASSVKKDLEAAKIPVVLFEGINAGDSDYSAIITKLKSQGVDFVYFGGYHPEMGLLLRQAR
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
EQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNG
HCCCEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
PFQMPAYAAVKIIADSIAGAKTTDPTKVAAYMHKTTFDTPIGKVAYDAQGDLKAFKFVVY
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEEE
TWHKDATKTAAK
EECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]