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The map label for this gene is rbsB [H]

Identifier: 189353491

GI number: 189353491

Start: 1709459

End: 1710400

Strand: Reverse

Name: rbsB [H]

Synonym: BMULJ_04735

Alternate gene names: 189353491

Gene position: 1710400-1709459 (Counterclockwise)

Preceding gene: 189353492

Following gene: 189353490

Centisome position: 69.16

GC content: 66.45

Gene sequence:

>942_bases
ATGATGCAACCCTCGTTCGCTTCACCGTTCGGCCGCACCGTTCGCGTCGCGGCTGTCGCGGCGCTCGCCGTCGCGGCCGT
GCTGCCCGCCGCCGCGCAGGCGGCGCCGCTGCGGATCGGCATGACCTTCCAGGAGTTGAACAACCCGTACTTCGTGACGA
TGCAAAAGGCGCTGAACGAGGCCGCGGCATCGATCGGCGCGCAGGTCGTCGTCACCGACGCGCACCACGACGTGACCAAG
CAGGTCAGCGACGTCGAGGACATGCTGCAGAAGAAGATCGACATCCTGCTCGTCAATCCGACCGATTCGACCGGCATCCA
GTCGGCGATCACGCAGGCGAAGAAGGCCGGTGCGGTCGTCGTGGCCGTCGACGCGAACGCGAACGGTCCCGTCGATTCGT
TTGTCGGCTCGAAGAACTACGACGCGGGCGTGATGTCGTGCGACTACCTCGCGAAAGCGATCGGCGGCAGCGGCGAGGTC
GCGATCCTCGACGGGATTCCGGTCGTGCCGATCCTCGAGCGCGTGCGCGGCTGCAAGGCCGGGCTCGCGAAATATCCGAA
CGTGAAGCTCGTCGACACGCAGAACGGCAAGCAGGAGCGCGCGACCGCGCTGTCGGTGACCGAGAACATGATCTCCGCGC
ATCCGAAGCTGAAGGGCATCTTCAGCGTGAACGACGGCGGTTCGATGGGCGCGCTCGCCGCGATCGAGAGTTCCGGCAAG
GACATCAAGCTGACGAGCGTCGATGGCGCACCGGAGGCGATCGCCGCGATCCAGAAGCCGAACTCGAAGTTCATCGAGAC
GACCGCGCAGTTCCCGGCCGACCAGGTGCGCATCGCGCTCGGCATCGCGATCGCGCGCAAGTGGGGGGCGACGGTGCCGA
AGGCGATTCCGGTCGACGTGAAGGTCGTCGACCGCGGCAACGCGAAGGGCTTCAGCTGGTGA

Upstream 100 bases:

>100_bases
CGCTTGTCCGCGAAAAATCGACGTCGCGGTTTTGCACGAGACCGGAGCACGCGCGCCCGCGCCGATTCCGGCCGATATCG
AAGACCCAGGAGACACCGTC

Downstream 100 bases:

>100_bases
ACGTGCGACGCGATGCGAGGGGCGCGTGCGCCCTTCGCGCGGAGAAGCCGATGGATACGATACTCAGGCTCAGCCAGATC
ACGAAAAGCTTTCCGGGCGT

Product: simple sugar transport system substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MMQPSFASPFGRTVRVAAVAALAVAAVLPAAAQAAPLRIGMTFQELNNPYFVTMQKALNEAAASIGAQVVVTDAHHDVTK
QVSDVEDMLQKKIDILLVNPTDSTGIQSAITQAKKAGAVVVAVDANANGPVDSFVGSKNYDAGVMSCDYLAKAIGGSGEV
AILDGIPVVPILERVRGCKAGLAKYPNVKLVDTQNGKQERATALSVTENMISAHPKLKGIFSVNDGGSMGALAAIESSGK
DIKLTSVDGAPEAIAAIQKPNSKFIETTAQFPADQVRIALGIAIARKWGATVPKAIPVDVKVVDRGNAKGFSW

Sequences:

>Translated_313_residues
MMQPSFASPFGRTVRVAAVAALAVAAVLPAAAQAAPLRIGMTFQELNNPYFVTMQKALNEAAASIGAQVVVTDAHHDVTK
QVSDVEDMLQKKIDILLVNPTDSTGIQSAITQAKKAGAVVVAVDANANGPVDSFVGSKNYDAGVMSCDYLAKAIGGSGEV
AILDGIPVVPILERVRGCKAGLAKYPNVKLVDTQNGKQERATALSVTENMISAHPKLKGIFSVNDGGSMGALAAIESSGK
DIKLTSVDGAPEAIAAIQKPNSKFIETTAQFPADQVRIALGIAIARKWGATVPKAIPVDVKVVDRGNAKGFSW
>Mature_313_residues
MMQPSFASPFGRTVRVAAVAALAVAAVLPAAAQAAPLRIGMTFQELNNPYFVTMQKALNEAAASIGAQVVVTDAHHDVTK
QVSDVEDMLQKKIDILLVNPTDSTGIQSAITQAKKAGAVVVAVDANANGPVDSFVGSKNYDAGVMSCDYLAKAIGGSGEV
AILDGIPVVPILERVRGCKAGLAKYPNVKLVDTQNGKQERATALSVTENMISAHPKLKGIFSVNDGGSMGALAAIESSGK
DIKLTSVDGAPEAIAAIQKPNSKFIETTAQFPADQVRIALGIAIARKWGATVPKAIPVDVKVVDRGNAKGFSW

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=290, Percent_Identity=32.7586206896552, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790526, Length=296, Percent_Identity=28.3783783783784, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32489; Mature: 32489

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQPSFASPFGRTVRVAAVAALAVAAVLPAAAQAAPLRIGMTFQELNNPYFVTMQKALNE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEHHHHHHH
AAASIGAQVVVTDAHHDVTKQVSDVEDMLQKKIDILLVNPTDSTGIQSAITQAKKAGAVV
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCEEE
VAVDANANGPVDSFVGSKNYDAGVMSCDYLAKAIGGSGEVAILDGIPVVPILERVRGCKA
EEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
GLAKYPNVKLVDTQNGKQERATALSVTENMISAHPKLKGIFSVNDGGSMGALAAIESSGK
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCC
DIKLTSVDGAPEAIAAIQKPNSKFIETTAQFPADQVRIALGIAIARKWGATVPKAIPVDV
EEEEEECCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
KVVDRGNAKGFSW
EEEECCCCCCCCC
>Mature Secondary Structure
MMQPSFASPFGRTVRVAAVAALAVAAVLPAAAQAAPLRIGMTFQELNNPYFVTMQKALNE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEHHHHHHH
AAASIGAQVVVTDAHHDVTKQVSDVEDMLQKKIDILLVNPTDSTGIQSAITQAKKAGAVV
HHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCEEE
VAVDANANGPVDSFVGSKNYDAGVMSCDYLAKAIGGSGEVAILDGIPVVPILERVRGCKA
EEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
GLAKYPNVKLVDTQNGKQERATALSVTENMISAHPKLKGIFSVNDGGSMGALAAIESSGK
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCC
DIKLTSVDGAPEAIAAIQKPNSKFIETTAQFPADQVRIALGIAIARKWGATVPKAIPVDV
EEEEEECCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEE
KVVDRGNAKGFSW
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]