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The map label for this gene is shiA [H]

Identifier: 189353461

GI number: 189353461

Start: 1679848

End: 1681167

Strand: Direct

Name: shiA [H]

Synonym: BMULJ_04705

Alternate gene names: 189353461

Gene position: 1679848-1681167 (Clockwise)

Preceding gene: 189353460

Following gene: 189353462

Centisome position: 67.92

GC content: 71.36

Gene sequence:

>1320_bases
ATGCCCGACACCATGAGCACGCTCGCCCAACCCGCCGTCCACGCACCGCACCGCGCCCGCAACAGCCGACTCGCGACCGC
GAGCATGATCGGCACGTCGCTCGAGTGGTACGACTTCACGATCTACAACACGCTCGCCGCGCTCGTCTTCAATCGGCTGT
TCTTTCCGTCCGTCGATCCGCTCGCCGGCACGATCCTCGCGTTCTCGACCTACGCGGTCGGCTACGTGTCGCGTCCGCTC
GGCGGCTTCGTGTTCGGCAATCTCGGCGACCGGATCGGCCGCCGCGCGGTGCTGATGCTCACGCTCGTGCTGATGGGCGT
GACGACCGCGCTGATGGGCGCGCTGCCGACCTATGCGACGGCCGGCATCCTGAGCCCGATCCTGCTCGTCGCGCTGCGCT
TCGTGCAGGGCGTCGCGCTCGGCGGCGAATGGGCCGGCGCCGTACTGCTGTCGGTCGAGCACGGCGATCAGAAGCGGCGC
GGGCTGTCCGCGTCGTGGACGCAGGTCGGCCCGTCGTTCGGCACGCTGCTCGGCACCGGCTGCATCGCGCTCGTCACGCT
GTCGACGACGCCGGACGGCTTTCTCGCGTGGGGCTGGCGGCTGCCGTTCGCGGCCAGCGCGCTGCTCGTCGCGTTCGGCT
TCTGGGTGCGGCGCGGCGTCGACGAAACGCCGCAGTTCGAGCAGCTCGCCGAGTCGCACGCGACGGCCGACGTGCCGGTC
GCCGAGGTGCTGACGTGTCACTGGCGCCGGCTGCTCGTCGCCGGCGGCGCGCGGATCGGCTCCGATGTGCTGTACGCGCT
GATCGTCGTCTTCACGCTGACCTACGTGACCACCGTGCTGCACCTGTCGCGGCCGCTCGCGCTGACGGCGGTGATGATCG
GCACCGCCTGCAACGCGATCGCGGTGCCGTGCTTCGGCGCGCTGTCGGACCGCTTCGGCCGCCGCCCCGTCTATCTGGCC
GGCGCGTTCGCCGGGATCGTATGGGCGTTCGCGTTCTTCGCGCTGCTCGATTCCGCGCGGCCGGCCGCGATCGTCGCAGC
CGTCGCGATCGGCCTCGTGATCCATGCGGTGATGTACGGGCCGCAAGGCGCGTTCGTCACCGAACAGTTTCCGACGCGCG
TGCGCTATGCGGGCTCGTCGCTCGCGTACACGCTGGCCGGCATCGTCGGCGGCGGCTTCGCGCCGCTCGTGATTGCCGCG
CTGTTCCGGCAGACCGGCACGACGACCGCCGTGTCGCTGTATGTGTCGGCCGCGCTCGCGGTGACGGCCGTCGCGCTCGT
CGTCGCGCGCGAAACCGCGCACCGGCCGCTCGCCGACTGA

Upstream 100 bases:

>100_bases
GCGAGCCTGCCCCGCAAGCGGCCGCCGCACTGCCCGCCTGAGCACCGAAGTCCGGCGCACATCGCCGGTTCCCGTTCGCC
CGCCCCGAGTTCGTCACGAC

Downstream 100 bases:

>100_bases
CCGCGGCCGCGTGCCGTTCCGCTCTCTCGCGTTTCATCCGGATACCTGCCCATGCAAGTCCTGTCGTTCAACGTGATTCC
CGTACACCAGCCGCCGGCCG

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MPDTMSTLAQPAVHAPHRARNSRLATASMIGTSLEWYDFTIYNTLAALVFNRLFFPSVDPLAGTILAFSTYAVGYVSRPL
GGFVFGNLGDRIGRRAVLMLTLVLMGVTTALMGALPTYATAGILSPILLVALRFVQGVALGGEWAGAVLLSVEHGDQKRR
GLSASWTQVGPSFGTLLGTGCIALVTLSTTPDGFLAWGWRLPFAASALLVAFGFWVRRGVDETPQFEQLAESHATADVPV
AEVLTCHWRRLLVAGGARIGSDVLYALIVVFTLTYVTTVLHLSRPLALTAVMIGTACNAIAVPCFGALSDRFGRRPVYLA
GAFAGIVWAFAFFALLDSARPAAIVAAVAIGLVIHAVMYGPQGAFVTEQFPTRVRYAGSSLAYTLAGIVGGGFAPLVIAA
LFRQTGTTTAVSLYVSAALAVTAVALVVARETAHRPLAD

Sequences:

>Translated_439_residues
MPDTMSTLAQPAVHAPHRARNSRLATASMIGTSLEWYDFTIYNTLAALVFNRLFFPSVDPLAGTILAFSTYAVGYVSRPL
GGFVFGNLGDRIGRRAVLMLTLVLMGVTTALMGALPTYATAGILSPILLVALRFVQGVALGGEWAGAVLLSVEHGDQKRR
GLSASWTQVGPSFGTLLGTGCIALVTLSTTPDGFLAWGWRLPFAASALLVAFGFWVRRGVDETPQFEQLAESHATADVPV
AEVLTCHWRRLLVAGGARIGSDVLYALIVVFTLTYVTTVLHLSRPLALTAVMIGTACNAIAVPCFGALSDRFGRRPVYLA
GAFAGIVWAFAFFALLDSARPAAIVAAVAIGLVIHAVMYGPQGAFVTEQFPTRVRYAGSSLAYTLAGIVGGGFAPLVIAA
LFRQTGTTTAVSLYVSAALAVTAVALVVARETAHRPLAD
>Mature_438_residues
PDTMSTLAQPAVHAPHRARNSRLATASMIGTSLEWYDFTIYNTLAALVFNRLFFPSVDPLAGTILAFSTYAVGYVSRPLG
GFVFGNLGDRIGRRAVLMLTLVLMGVTTALMGALPTYATAGILSPILLVALRFVQGVALGGEWAGAVLLSVEHGDQKRRG
LSASWTQVGPSFGTLLGTGCIALVTLSTTPDGFLAWGWRLPFAASALLVAFGFWVRRGVDETPQFEQLAESHATADVPVA
EVLTCHWRRLLVAGGARIGSDVLYALIVVFTLTYVTTVLHLSRPLALTAVMIGTACNAIAVPCFGALSDRFGRRPVYLAG
AFAGIVWAFAFFALLDSARPAAIVAAVAIGLVIHAVMYGPQGAFVTEQFPTRVRYAGSSLAYTLAGIVGGGFAPLVIAAL
FRQTGTTTAVSLYVSAALAVTAVALVVARETAHRPLAD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=40.2380952380952, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1789941, Length=442, Percent_Identity=38.6877828054299, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=33.6470588235294, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=30.3827751196172, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082404, Length=418, Percent_Identity=22.7272727272727, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082231, Length=261, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46221; Mature: 46090

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTMSTLAQPAVHAPHRARNSRLATASMIGTSLEWYDFTIYNTLAALVFNRLFFPSVDP
CCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
LAGTILAFSTYAVGYVSRPLGGFVFGNLGDRIGRRAVLMLTLVLMGVTTALMGALPTYAT
HHHHHHHHHHHHHHHHHCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGILSPILLVALRFVQGVALGGEWAGAVLLSVEHGDQKRRGLSASWTQVGPSFGTLLGTG
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHCCCCCCHHHCCCHHHHHHHCC
CIALVTLSTTPDGFLAWGWRLPFAASALLVAFGFWVRRGVDETPQFEQLAESHATADVPV
HHEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCH
AEVLTCHWRRLLVAGGARIGSDVLYALIVVFTLTYVTTVLHLSRPLALTAVMIGTACNAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AVPCFGALSDRFGRRPVYLAGAFAGIVWAFAFFALLDSARPAAIVAAVAIGLVIHAVMYG
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PQGAFVTEQFPTRVRYAGSSLAYTLAGIVGGGFAPLVIAALFRQTGTTTAVSLYVSAALA
CCCCEECCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VTAVALVVARETAHRPLAD
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PDTMSTLAQPAVHAPHRARNSRLATASMIGTSLEWYDFTIYNTLAALVFNRLFFPSVDP
CCHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
LAGTILAFSTYAVGYVSRPLGGFVFGNLGDRIGRRAVLMLTLVLMGVTTALMGALPTYAT
HHHHHHHHHHHHHHHHHCCCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGILSPILLVALRFVQGVALGGEWAGAVLLSVEHGDQKRRGLSASWTQVGPSFGTLLGTG
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHCCCCCCHHHCCCHHHHHHHCC
CIALVTLSTTPDGFLAWGWRLPFAASALLVAFGFWVRRGVDETPQFEQLAESHATADVPV
HHEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCH
AEVLTCHWRRLLVAGGARIGSDVLYALIVVFTLTYVTTVLHLSRPLALTAVMIGTACNAI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AVPCFGALSDRFGRRPVYLAGAFAGIVWAFAFFALLDSARPAAIVAAVAIGLVIHAVMYG
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PQGAFVTEQFPTRVRYAGSSLAYTLAGIVGGGFAPLVIAALFRQTGTTTAVSLYVSAALA
CCCCEECCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VTAVALVVARETAHRPLAD
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]