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The map label for this gene is proP [H]

Identifier: 189353326

GI number: 189353326

Start: 1563154

End: 1564476

Strand: Reverse

Name: proP [H]

Synonym: BMULJ_04566

Alternate gene names: 189353326

Gene position: 1564476-1563154 (Counterclockwise)

Preceding gene: 189353328

Following gene: 189353325

Centisome position: 63.26

GC content: 68.25

Gene sequence:

>1323_bases
ATGCGCGCTGATTCAGGTCGGAGCACGCGGCATCGGCAAGTGGTTGCCGCTGTCATCGGCAATACCCTCGAGTGGTACGA
CTTCATCGTTTACGGGTTCTTCTCCGGCATCATCGCGAGACTGTTCTTTCCGGCAGACAGCCAGTACGCATCGCTGCTGA
TGTCGCTCGCGACGTTCGGCGTCGGCTTCTTCATGCGTCCGGTGGGCGGCATCCTGCTCGGGCTCTATGCGGACCGCAAG
GGCCGCAAGGCGGCGATGCAGCTGATCATCCTGCTGATGACGCTGTCGATCGCGATGATCACGTTCGCGCCGACGTATGC
GGCGATCGGCCCTGCCGCGCCGATGCTGATCGTGATCGCGCGGCTGCTGCAGGGCTTCGCGACCGGCGGCGAATACGCGA
GCGCGACGTCGTTCCTCGTCGAAAGCGCGCCCGCGCACCGGCGCGGCCTGTACGGATCGTGGCAACTGATCGGCCAGTGT
CTTGCGGTGTTTTCGGGCGCGGCGATCGGCGCGTGGGCGACGCAGTCGCTGTCGGCCGGTGCACTCGACAGCTGGGGCTG
GCGCGTGCCGTTCGCGCTCGGGCTGCTGATCGGGCCGGTCGGCCTGTGGATCCGCCGCCACATGGAAGAGACCGACGCGT
TCGTCGAAGCCAGCAAGTCGCGGGCCGAGCCGCCCGCCAGCGTCGCACGCGTGCTGCGCGACAACCTGCGCGGCGTGCTG
GTCTCGATGGGGCAGACGATCACGGGCACGGCCGTGTTCTATGTGATGCTCGTCAATATGCCGACGTTCGCGAACCGGAC
CTTCGGGCTGCCGCTCGATCAGGTGTTCCAGGCGCAGATGGCGGCGGTCGCGCTGCTGACGCTGACGATCCCGGCGGCGG
CGATGGTGTCCGATCGCATCGGTCGCCGGCCGGTGCTGCTCGCCGGCATGCTGGCACTGCTGACGGTCACCTATCCGCTG
TTCTCGTGGCTGGCCGCCGCGCCGAGCATCGGCCGGCTGCTCGTTATGCAGCTGGTGATCTGCACGATCATCGGCGTGAC
CTACGGCCCCGCGCCGACGGCGCTCGCCGAGCAGTTCGCGACGCGCATGCGGTCGACCGGCATTGCGCTCGCGTACAACA
TCGCGGTGATGATCTTCGGCGGCTTCGCGCCGTTCATCGTCACATGGCTGACGCGCGCGAGCGGCTCGCCCGTCGCGCCT
GCCTGGTACGTGCTGTTCGCGGCGTCGTTCGGGCTGGCCGCGAGCGTGTTCATGCGCGACGGCGCGCCATGCGTGCTGGC
GCGCCGGCAGTTTCGCGGCGAGCCGGTCACCAGCGCTGAGTAA

Upstream 100 bases:

>100_bases
GCGGGCCGGCGCACACCAACGGGCAGGCCGCATGCGCCGTGCCGGCGGCATGGCGCGACGCATCGCGCGGCGCGGCCTCA
TGAGACTGCAGGAGACACGC

Downstream 100 bases:

>100_bases
AGCGGCTTGGCGCCCGTGCCGGGCGGCTGGCTGCGCGGTCATGGCACATGAGTGCGGGCCGAAGGGCGACTATCGGCTAT
CGGACCGTTCGATGGCTCCA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRADSGRSTRHRQVVAAVIGNTLEWYDFIVYGFFSGIIARLFFPADSQYASLLMSLATFGVGFFMRPVGGILLGLYADRK
GRKAAMQLIILLMTLSIAMITFAPTYAAIGPAAPMLIVIARLLQGFATGGEYASATSFLVESAPAHRRGLYGSWQLIGQC
LAVFSGAAIGAWATQSLSAGALDSWGWRVPFALGLLIGPVGLWIRRHMEETDAFVEASKSRAEPPASVARVLRDNLRGVL
VSMGQTITGTAVFYVMLVNMPTFANRTFGLPLDQVFQAQMAAVALLTLTIPAAAMVSDRIGRRPVLLAGMLALLTVTYPL
FSWLAAAPSIGRLLVMQLVICTIIGVTYGPAPTALAEQFATRMRSTGIALAYNIAVMIFGGFAPFIVTWLTRASGSPVAP
AWYVLFAASFGLAASVFMRDGAPCVLARRQFRGEPVTSAE

Sequences:

>Translated_440_residues
MRADSGRSTRHRQVVAAVIGNTLEWYDFIVYGFFSGIIARLFFPADSQYASLLMSLATFGVGFFMRPVGGILLGLYADRK
GRKAAMQLIILLMTLSIAMITFAPTYAAIGPAAPMLIVIARLLQGFATGGEYASATSFLVESAPAHRRGLYGSWQLIGQC
LAVFSGAAIGAWATQSLSAGALDSWGWRVPFALGLLIGPVGLWIRRHMEETDAFVEASKSRAEPPASVARVLRDNLRGVL
VSMGQTITGTAVFYVMLVNMPTFANRTFGLPLDQVFQAQMAAVALLTLTIPAAAMVSDRIGRRPVLLAGMLALLTVTYPL
FSWLAAAPSIGRLLVMQLVICTIIGVTYGPAPTALAEQFATRMRSTGIALAYNIAVMIFGGFAPFIVTWLTRASGSPVAP
AWYVLFAASFGLAASVFMRDGAPCVLARRQFRGEPVTSAE
>Mature_440_residues
MRADSGRSTRHRQVVAAVIGNTLEWYDFIVYGFFSGIIARLFFPADSQYASLLMSLATFGVGFFMRPVGGILLGLYADRK
GRKAAMQLIILLMTLSIAMITFAPTYAAIGPAAPMLIVIARLLQGFATGGEYASATSFLVESAPAHRRGLYGSWQLIGQC
LAVFSGAAIGAWATQSLSAGALDSWGWRVPFALGLLIGPVGLWIRRHMEETDAFVEASKSRAEPPASVARVLRDNLRGVL
VSMGQTITGTAVFYVMLVNMPTFANRTFGLPLDQVFQAQMAAVALLTLTIPAAAMVSDRIGRRPVLLAGMLALLTVTYPL
FSWLAAAPSIGRLLVMQLVICTIIGVTYGPAPTALAEQFATRMRSTGIALAYNIAVMIFGGFAPFIVTWLTRASGSPVAP
AWYVLFAASFGLAASVFMRDGAPCVLARRQFRGEPVTSAE

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=31.0747663551402, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788942, Length=406, Percent_Identity=33.9901477832512, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=29.6983758700696, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789941, Length=398, Percent_Identity=31.1557788944724, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=28.3185840707965, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=424, Percent_Identity=21.6981132075472, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47115; Mature: 47115

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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CCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]