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The map label for this gene is hcaD [H]

Identifier: 189353268

GI number: 189353268

Start: 1505945

End: 1507180

Strand: Reverse

Name: hcaD [H]

Synonym: BMULJ_04508

Alternate gene names: 189353268

Gene position: 1507180-1505945 (Counterclockwise)

Preceding gene: 189353269

Following gene: 189353267

Centisome position: 60.94

GC content: 69.82

Gene sequence:

>1236_bases
ATGACCGACGCGAACACCGATGCGCCGCGCACGATCGTCGTGATCGGCGGCGGGCAGGCGGCCGGCTGGGTACTGAAGAC
GCTGCGCGCGGAAGGCTATGCGGGCCGTCTCGTGATGATCGCCGACGAGCCGCATCTGCCGTACGAGCGTCCGCCGTTGT
CGAAGGCCGTGCTGGCCGGCGACGCGGACATCGAAACCGTGCGCGTCGTGCGCCCGGACGAGTTCGACGCGCTGAACGTC
GAGGCCTGGCAGCCCGAACGCGCGGCATCGATCGACCGCGCGCGGCGCGTGGTGAAGACCGCGAGCGGTCGCGAGATCGA
ATACGACCGCCTCGTGATCGCGACCGGCGGCACGTCGCGCCGGCTGCCCGACGCGATCGTCCATACGTCGAACCTGCACT
ACCTGCGCACGCTCGACGAGGCCGCCGCGCTCGGCGAGAAGCTGCGCGCGAGTCGCCGCGTGCTCGTGATCGGCGGTGGC
TGGATCGGCCTCGAAGTCGCGGCGACCGCGCGCAAGCTCGGCGTCGACGCGGTCGTCGTCGAAGGCGCGCCGCGGCTGTG
CGGCCGCTCGGTGCCGCCGATCGTGTCGGATTTCCTGTTCGACCTGCATCGCGCGAACGGCGTCGACGTGCGGCTCGGTG
CTGCGCTCGCGTCGCTCGACGCGCAGCCGGACGACACGTCGAAAGTACGCGCGACGCTGGCCGACGGCACGACGATCGAC
GCCGATTTCGCGGTGGCCGGCATCGGTCTCGCGCTGAACGCGTCGCTGGCGAGCGACGCGGGGCTGGCCGTCGACGACGG
CATCGTCGTCGACGAGTTCGGTGCGACGAGCGATCCGGCGATCTTCGCGTGCGGCGACGTCGCGAATCATCACAACGGCT
GGCTGAAGCGGCGCGTGCGGCTCGAATCGTGGGCCAATGCGCAGAACCAGGCGATCGCGGCCGCGAAGGCGGTGCTCGGC
GTGCGTGCGGCGTACGCCGAAATTCCGTGGTTCTGGTCCGATCAGTACGACGTGAACCTGCAGATCCTCGGCGATCTGCC
GGCCGATGCGCAGCTCGTCGTGCGCGGCGATCTCGCCGCGCGCCGCGCGACGCTGTTTTTCCTCGGCGATGGACATGTGA
GAGGTGTCGTCGCCATCAACAACGCGCGCGAACTGAAGCTTGCGCGCAAGTGGATGAACCAGGGCCGGGCAGTGGATAGG
GAAGCCCTGGCAGACACGACCAAGGCGCTTGCCTGA

Upstream 100 bases:

>100_bases
TGTTCGCCGAGTGGGCGGTCGACGGCGGCCTCGACGGCAACACGCGCCGGCAGAAGGACGCGAAGGCGCCGGAAGGCAAG
TTCATGACGGAGCGCAAATC

Downstream 100 bases:

>100_bases
GCGGCCAGACCCACACGAGTTCCAGGAGACACCCCGCATGAGCACTTTCGACAACGTCGTGGCCGGTCGGGCCACGATGG
AAGCTGCCGCCTCCACGCCC

Product: ferredoxin-NAD+ reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTDANTDAPRTIVVIGGGQAAGWVLKTLRAEGYAGRLVMIADEPHLPYERPPLSKAVLAGDADIETVRVVRPDEFDALNV
EAWQPERAASIDRARRVVKTASGREIEYDRLVIATGGTSRRLPDAIVHTSNLHYLRTLDEAAALGEKLRASRRVLVIGGG
WIGLEVAATARKLGVDAVVVEGAPRLCGRSVPPIVSDFLFDLHRANGVDVRLGAALASLDAQPDDTSKVRATLADGTTID
ADFAVAGIGLALNASLASDAGLAVDDGIVVDEFGATSDPAIFACGDVANHHNGWLKRRVRLESWANAQNQAIAAAKAVLG
VRAAYAEIPWFWSDQYDVNLQILGDLPADAQLVVRGDLAARRATLFFLGDGHVRGVVAINNARELKLARKWMNQGRAVDR
EALADTTKALA

Sequences:

>Translated_411_residues
MTDANTDAPRTIVVIGGGQAAGWVLKTLRAEGYAGRLVMIADEPHLPYERPPLSKAVLAGDADIETVRVVRPDEFDALNV
EAWQPERAASIDRARRVVKTASGREIEYDRLVIATGGTSRRLPDAIVHTSNLHYLRTLDEAAALGEKLRASRRVLVIGGG
WIGLEVAATARKLGVDAVVVEGAPRLCGRSVPPIVSDFLFDLHRANGVDVRLGAALASLDAQPDDTSKVRATLADGTTID
ADFAVAGIGLALNASLASDAGLAVDDGIVVDEFGATSDPAIFACGDVANHHNGWLKRRVRLESWANAQNQAIAAAKAVLG
VRAAYAEIPWFWSDQYDVNLQILGDLPADAQLVVRGDLAARRATLFFLGDGHVRGVVAINNARELKLARKWMNQGRAVDR
EALADTTKALA
>Mature_410_residues
TDANTDAPRTIVVIGGGQAAGWVLKTLRAEGYAGRLVMIADEPHLPYERPPLSKAVLAGDADIETVRVVRPDEFDALNVE
AWQPERAASIDRARRVVKTASGREIEYDRLVIATGGTSRRLPDAIVHTSNLHYLRTLDEAAALGEKLRASRRVLVIGGGW
IGLEVAATARKLGVDAVVVEGAPRLCGRSVPPIVSDFLFDLHRANGVDVRLGAALASLDAQPDDTSKVRATLADGTTIDA
DFAVAGIGLALNASLASDAGLAVDDGIVVDEFGATSDPAIFACGDVANHHNGWLKRRVRLESWANAQNQAIAAAKAVLGV
RAAYAEIPWFWSDQYDVNLQILGDLPADAQLVVRGDLAARRATLFFLGDGHVRGVVAINNARELKLARKWMNQGRAVDRE
ALADTTKALA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=406, Percent_Identity=26.3546798029557, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI21389617, Length=406, Percent_Identity=26.3546798029557, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI226437568, Length=406, Percent_Identity=26.3546798029557, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4757732, Length=346, Percent_Identity=27.4566473988439, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI22202629, Length=346, Percent_Identity=27.4566473988439, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=39.9503722084367, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789065, Length=309, Percent_Identity=30.42071197411, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789765, Length=286, Percent_Identity=27.972027972028, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=331, Percent_Identity=27.190332326284, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564386, Length=442, Percent_Identity=25.5656108597285, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639257, Length=417, Percent_Identity=24.9400479616307, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281359715, Length=417, Percent_Identity=24.9400479616307, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI281359713, Length=417, Percent_Identity=24.9400479616307, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639250, Length=417, Percent_Identity=24.9400479616307, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI18543267, Length=417, Percent_Identity=24.9400479616307, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24639252, Length=417, Percent_Identity=24.9400479616307, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24585130, Length=376, Percent_Identity=23.6702127659574, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581020, Length=373, Percent_Identity=26.8096514745308, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573993, Length=373, Percent_Identity=26.8096514745308, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43846; Mature: 43715

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDANTDAPRTIVVIGGGQAAGWVLKTLRAEGYAGRLVMIADEPHLPYERPPLSKAVLAG
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEEEC
DADIETVRVVRPDEFDALNVEAWQPERAASIDRARRVVKTASGREIEYDRLVIATGGTSR
CCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCCC
RLPDAIVHTSNLHYLRTLDEAAALGEKLRASRRVLVIGGGWIGLEVAATARKLGVDAVVV
CCCHHHEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEHHHHHHCCCCEEEE
EGAPRLCGRSVPPIVSDFLFDLHRANGVDVRLGAALASLDAQPDDTSKVRATLADGTTID
CCCHHHCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCEEEEECCCCEEC
ADFAVAGIGLALNASLASDAGLAVDDGIVVDEFGATSDPAIFACGDVANHHNGWLKRRVR
CCCEEEEECEEEECCCCCCCCCEEECCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHH
LESWANAQNQAIAAAKAVLGVRAAYAEIPWFWSDQYDVNLQILGDLPADAQLVVRGDLAA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEEEECCCCCCEEEEECCCCC
RRATLFFLGDGHVRGVVAINNARELKLARKWMNQGRAVDREALADTTKALA
CEEEEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TDANTDAPRTIVVIGGGQAAGWVLKTLRAEGYAGRLVMIADEPHLPYERPPLSKAVLAG
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEEEC
DADIETVRVVRPDEFDALNVEAWQPERAASIDRARRVVKTASGREIEYDRLVIATGGTSR
CCCCEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCCC
RLPDAIVHTSNLHYLRTLDEAAALGEKLRASRRVLVIGGGWIGLEVAATARKLGVDAVVV
CCCHHHEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEHHHHHHCCCCEEEE
EGAPRLCGRSVPPIVSDFLFDLHRANGVDVRLGAALASLDAQPDDTSKVRATLADGTTID
CCCHHHCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCEEEEECCCCEEC
ADFAVAGIGLALNASLASDAGLAVDDGIVVDEFGATSDPAIFACGDVANHHNGWLKRRVR
CCCEEEEECEEEECCCCCCCCCEEECCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHH
LESWANAQNQAIAAAKAVLGVRAAYAEIPWFWSDQYDVNLQILGDLPADAQLVVRGDLAA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEEEECCCCCCEEEEECCCCC
RRATLFFLGDGHVRGVVAINNARELKLARKWMNQGRAVDREALADTTKALA
CEEEEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]