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The map label for this gene is gcvA [H]

Identifier: 189353217

GI number: 189353217

Start: 1445186

End: 1446058

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_04455

Alternate gene names: 189353217

Gene position: 1446058-1445186 (Counterclockwise)

Preceding gene: 189353218

Following gene: 189353202

Centisome position: 58.47

GC content: 68.27

Gene sequence:

>873_bases
ATGCGCCGACTTCCGCCTTTGCACGCGCTGCAGATCTTCTCGACCGTCGCCCGTCACGGCAGCTTCACGCGCGCGGCCGA
GCAGCTGTGCGTGACGCAGGGCGCGGTGAGCCGGCAGATCCAGACGCTCGAGGCGCATTACGGCTTCCCGCTGTTCAAGC
GGCATGCGAAAGGACTGACGCTGACGGCCGAAGGCGAACAGCTGCTGCCGGTCGTCAACGAGAGCTTCGCGCGGATCGAG
GACATTTCGTCGAAGCTCACGCGGCAGCGCACCGACCTCGCGCTAAAGGTGCCGACCTGCGTGATGCGCTGGATGCTGCC
GCGCATCATGCGCTTTCAGGGCGAGCATCCCGATCTGCACGTCCAGATCACGACCGCATGGCAGCACGTGGTCGACTTCT
CGTCCGAGCCGTTCGACGCGGCGATCGTCTACGGCACGTCGCCGGGCGCCGGCGTGGTCGCGCTGCCGCTGTTCGGCGAG
CGGCTGACGCCCGTGTGCGCGCCCGAGCTGCGGCAAACGGTGCCGCTCGAGGCGGTCGACGACCTCGCGCGCCATACGCT
GCTGCATCCGACGCGCGACCATCGCGACTGGCGCGCATGGCTCGAGCACGCAGGCGTGCGCACGGTCGATCCCGAGCGCG
GGCCGACCTTCGACACGCTCGATCTCGCGACGAACGCCGCGATGCAAGGCTTCGGCGTCGCGATCGGCGACGTGACGCTC
GTCGACGACGACGTCAGTGCGCGCCGGCTCGAACGGCCGTTTGACATCGTGCTCGAGACGGGTGCGCGCTACTTCTTCGT
GTACCCGGAGAGCGGCAGCAGCCAGCAGAAGATCCGCGCGTTCAGCGACTGGATCGCGCGCAATCGCGATTGA

Upstream 100 bases:

>100_bases
GCGCGGCGCCGGCACTCGCGTCGAGCGGGCGTTTGCCTTATCGTGTCGGTCAGCGCGTGCCGCCGCCGGCACGCCGTCTT
TCCGCTCACTTCGCTTCGCC

Downstream 100 bases:

>100_bases
TGCGGCGCGGCGCGCGGCCGCCTGCTTACTGATACGTGACGATCGCGATCATTGGCGTGTGCTCGCCGCCCGGGCGCGTC
GGTAAAACGATTGCGCGCGA

Product: LysR family glycine cleavage system transcriptional activator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRRLPPLHALQIFSTVARHGSFTRAAEQLCVTQGAVSRQIQTLEAHYGFPLFKRHAKGLTLTAEGEQLLPVVNESFARIE
DISSKLTRQRTDLALKVPTCVMRWMLPRIMRFQGEHPDLHVQITTAWQHVVDFSSEPFDAAIVYGTSPGAGVVALPLFGE
RLTPVCAPELRQTVPLEAVDDLARHTLLHPTRDHRDWRAWLEHAGVRTVDPERGPTFDTLDLATNAAMQGFGVAIGDVTL
VDDDVSARRLERPFDIVLETGARYFFVYPESGSSQQKIRAFSDWIARNRD

Sequences:

>Translated_290_residues
MRRLPPLHALQIFSTVARHGSFTRAAEQLCVTQGAVSRQIQTLEAHYGFPLFKRHAKGLTLTAEGEQLLPVVNESFARIE
DISSKLTRQRTDLALKVPTCVMRWMLPRIMRFQGEHPDLHVQITTAWQHVVDFSSEPFDAAIVYGTSPGAGVVALPLFGE
RLTPVCAPELRQTVPLEAVDDLARHTLLHPTRDHRDWRAWLEHAGVRTVDPERGPTFDTLDLATNAAMQGFGVAIGDVTL
VDDDVSARRLERPFDIVLETGARYFFVYPESGSSQQKIRAFSDWIARNRD
>Mature_290_residues
MRRLPPLHALQIFSTVARHGSFTRAAEQLCVTQGAVSRQIQTLEAHYGFPLFKRHAKGLTLTAEGEQLLPVVNESFARIE
DISSKLTRQRTDLALKVPTCVMRWMLPRIMRFQGEHPDLHVQITTAWQHVVDFSSEPFDAAIVYGTSPGAGVVALPLFGE
RLTPVCAPELRQTVPLEAVDDLARHTLLHPTRDHRDWRAWLEHAGVRTVDPERGPTFDTLDLATNAAMQGFGVAIGDVTL
VDDDVSARRLERPFDIVLETGARYFFVYPESGSSQQKIRAFSDWIARNRD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=37.1527777777778, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=28.6713286713287, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=28.719723183391, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=26.2237762237762, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=25.8620689655172, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=31.1377245508982, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=214, Percent_Identity=28.9719626168224, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32587; Mature: 32587

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPPLHALQIFSTVARHGSFTRAAEQLCVTQGAVSRQIQTLEAHYGFPLFKRHAKGLT
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCE
LTAEGEQLLPVVNESFARIEDISSKLTRQRTDLALKVPTCVMRWMLPRIMRFQGEHPDLH
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
VQITTAWQHVVDFSSEPFDAAIVYGTSPGAGVVALPLFGERLTPVCAPELRQTVPLEAVD
EEEEHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHH
DLARHTLLHPTRDHRDWRAWLEHAGVRTVDPERGPTFDTLDLATNAAMQGFGVAIGDVTL
HHHHHHHCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHCCCEEECCEEE
VDDDVSARRLERPFDIVLETGARYFFVYPESGSSQQKIRAFSDWIARNRD
ECCCHHHHHHCCCHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRLPPLHALQIFSTVARHGSFTRAAEQLCVTQGAVSRQIQTLEAHYGFPLFKRHAKGLT
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCE
LTAEGEQLLPVVNESFARIEDISSKLTRQRTDLALKVPTCVMRWMLPRIMRFQGEHPDLH
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
VQITTAWQHVVDFSSEPFDAAIVYGTSPGAGVVALPLFGERLTPVCAPELRQTVPLEAVD
EEEEHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHH
DLARHTLLHPTRDHRDWRAWLEHAGVRTVDPERGPTFDTLDLATNAAMQGFGVAIGDVTL
HHHHHHHCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHCCCEEECCEEE
VDDDVSARRLERPFDIVLETGARYFFVYPESGSSQQKIRAFSDWIARNRD
ECCCHHHHHHCCCHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]