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The map label for this gene is emrB [H]

Identifier: 189353198

GI number: 189353198

Start: 1423372

End: 1424826

Strand: Direct

Name: emrB [H]

Synonym: BMULJ_04434

Alternate gene names: 189353198

Gene position: 1423372-1424826 (Clockwise)

Preceding gene: 189353197

Following gene: 189353200

Centisome position: 57.55

GC content: 70.58

Gene sequence:

>1455_bases
GTGAGCGACGAACGTACGCAACCGGCGCCGGCCCGCGTGGGCGGCGGACTCGATCCGTCGATCTGGAAGGTCAGCGCGGT
GGCGATGCTCGGCTCGCTGCTGTCGCAGCTCGACGCGACGATCGTCAACGTGTCGCTGTCGAGCCTCGCGACGGATCTGC
AGACGAGCCTGTCGACGATCCAGTGGGTCACGAGCGGCTATCTGCTCGCCCTGACGCTGGTGCTGCCGCTGAACGGCTGG
CTCGTCGACCGGATCGGCGCGAAGGCGCTGTACTTGTGGTGCTTCTCCGCATTCACGCTGACGTCGGCGCTCTGCGGGCT
CGCGTGGTCCGCGCCGTCGCTGATCGCGTTTCGCGCGTTGCAAGGCATGAGTGGCGGACTGCTCGCCCCGATGGCGCAGA
TGATGATCGCGCGCGTCGCGGGCCAGCAGATGGCGCGCGTGATCGGCTATGCGGCGGTGCCAGTGCTGCTCGCGCCGATC
CTCGGCCCGGTCGTCGCGGGCGCGATTCTGCAACACACGTCGTGGCGCTGGCTGTTTCTCGTGAACCTGCCGGTCGGCGT
GCTCGCGCTTGCGCTCGCTGCCTGGTTCCTGCCCGGCGATCGCGAAGAAACGCAGCCGCGAGCGCTCGACTGGATCGGGC
TGTCGCTGTTGTCGCCGGGGCTCGTGCTGTTCCTGTACGGCGTCGAGCGCATCGGCAATGCGCTCGGCATCGCGGCACTC
GCCGCCGCGGCGCTGCTGCTCGTTGCCTTCCTGCGCGTCGAAACGCGCATGGGCGACCGCGCGCTGCTCGATCTCGCGCT
GTTTCGCCGCAAGGTGTTCGGCGCGGCGGCCTCGACGCAGTTCCTGTCGAACGGCGCGCTGTTCGCGGGCCAGATGCTGA
TCCCGGTGTTCCTGATCCAGGCATGCGGCCGTTCGCCCGGCGAGATGGGCTGGCTGCTCGCGCCGATGGGCCTCGGCATG
CTCGTCACCTATCCGTCGATGGGCGCGCTGACGGGCCGCTTCGGCGTGCGGCGGCTCGCCGCCGCGGGCGCGATGCTCGC
GCTGCTCGCGACGCTGCCGTTCGTCTATCTCGCGCTGCGCGGCTACGATCCCGTCGTGCTCGTGCCTGCGCTATTCCTGC
GCGGGATGGGACAGAGCGCGATCGGCGCGCCGTCCATTACGGCCGCTTATGCGTCGGTCGAACGCCGCGACCTGCCGATG
GCGACCACGTCGCTCAATATCGTGCAGCGCCTCGGCGGGCCGACGTTGACGACGCTCTGCACGCTGTTTCTCGCCTGGCG
GCTGCAGGCCGATCACGTGGCGGGCGGCGACGCGCACGGGACCGCTTACGCCTGGGCGTTCGGCCTGCTGTGCGTGCTGC
ACGCGTCGAGTTTCGCCACTGCGCTGCGCCTGCCGCTGTGGTCCGCCGGCGCAGGCGGCCGCCGCGCGGCACCGGCGTCA
AGCGGCTCGCGCTGA

Upstream 100 bases:

>100_bases
ACGCGGACGCCCATGCGAACAGCGTCGAGCGCCTGTTCCCGCGCCTCGGCGAGACGGGCTCGACGCAGGACATCGTCGCG
CTGCTCGACCGGCGCGGCGC

Downstream 100 bases:

>100_bases
ACGCGCCGAGTCGCACCACGTCGCGCCGCGTTGCGCCGACTGTGCGGCGACGAACCGCGCGATCGCTTCACGGCAGCCAC
ATCGGCACGATCGAGCCCAC

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSDERTQPAPARVGGGLDPSIWKVSAVAMLGSLLSQLDATIVNVSLSSLATDLQTSLSTIQWVTSGYLLALTLVLPLNGW
LVDRIGAKALYLWCFSAFTLTSALCGLAWSAPSLIAFRALQGMSGGLLAPMAQMMIARVAGQQMARVIGYAAVPVLLAPI
LGPVVAGAILQHTSWRWLFLVNLPVGVLALALAAWFLPGDREETQPRALDWIGLSLLSPGLVLFLYGVERIGNALGIAAL
AAAALLLVAFLRVETRMGDRALLDLALFRRKVFGAAASTQFLSNGALFAGQMLIPVFLIQACGRSPGEMGWLLAPMGLGM
LVTYPSMGALTGRFGVRRLAAAGAMLALLATLPFVYLALRGYDPVVLVPALFLRGMGQSAIGAPSITAAYASVERRDLPM
ATTSLNIVQRLGGPTLTTLCTLFLAWRLQADHVAGGDAHGTAYAWAFGLLCVLHASSFATALRLPLWSAGAGGRRAAPAS
SGSR

Sequences:

>Translated_484_residues
MSDERTQPAPARVGGGLDPSIWKVSAVAMLGSLLSQLDATIVNVSLSSLATDLQTSLSTIQWVTSGYLLALTLVLPLNGW
LVDRIGAKALYLWCFSAFTLTSALCGLAWSAPSLIAFRALQGMSGGLLAPMAQMMIARVAGQQMARVIGYAAVPVLLAPI
LGPVVAGAILQHTSWRWLFLVNLPVGVLALALAAWFLPGDREETQPRALDWIGLSLLSPGLVLFLYGVERIGNALGIAAL
AAAALLLVAFLRVETRMGDRALLDLALFRRKVFGAAASTQFLSNGALFAGQMLIPVFLIQACGRSPGEMGWLLAPMGLGM
LVTYPSMGALTGRFGVRRLAAAGAMLALLATLPFVYLALRGYDPVVLVPALFLRGMGQSAIGAPSITAAYASVERRDLPM
ATTSLNIVQRLGGPTLTTLCTLFLAWRLQADHVAGGDAHGTAYAWAFGLLCVLHASSFATALRLPLWSAGAGGRRAAPAS
SGSR
>Mature_483_residues
SDERTQPAPARVGGGLDPSIWKVSAVAMLGSLLSQLDATIVNVSLSSLATDLQTSLSTIQWVTSGYLLALTLVLPLNGWL
VDRIGAKALYLWCFSAFTLTSALCGLAWSAPSLIAFRALQGMSGGLLAPMAQMMIARVAGQQMARVIGYAAVPVLLAPIL
GPVVAGAILQHTSWRWLFLVNLPVGVLALALAAWFLPGDREETQPRALDWIGLSLLSPGLVLFLYGVERIGNALGIAALA
AAALLLVAFLRVETRMGDRALLDLALFRRKVFGAAASTQFLSNGALFAGQMLIPVFLIQACGRSPGEMGWLLAPMGLGML
VTYPSMGALTGRFGVRRLAAAGAMLALLATLPFVYLALRGYDPVVLVPALFLRGMGQSAIGAPSITAAYASVERRDLPMA
TTSLNIVQRLGGPTLTTLCTLFLAWRLQADHVAGGDAHGTAYAWAFGLLCVLHASSFATALRLPLWSAGAGGRRAAPASS
GSR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=317, Percent_Identity=29.0220820189274, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788392, Length=389, Percent_Identity=31.3624678663239, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789042, Length=410, Percent_Identity=27.3170731707317, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1788710, Length=447, Percent_Identity=27.0693512304251, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87081983, Length=344, Percent_Identity=30.2325581395349, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=138, Percent_Identity=28.2608695652174, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50794; Mature: 50663

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDERTQPAPARVGGGLDPSIWKVSAVAMLGSLLSQLDATIVNVSLSSLATDLQTSLSTI
CCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWVTSGYLLALTLVLPLNGWLVDRIGAKALYLWCFSAFTLTSALCGLAWSAPSLIAFRAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QGMSGGLLAPMAQMMIARVAGQQMARVIGYAAVPVLLAPILGPVVAGAILQHTSWRWLFL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VNLPVGVLALALAAWFLPGDREETQPRALDWIGLSLLSPGLVLFLYGVERIGNALGIAAL
ECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAALLLVAFLRVETRMGDRALLDLALFRRKVFGAAASTQFLSNGALFAGQMLIPVFLIQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ACGRSPGEMGWLLAPMGLGMLVTYPSMGALTGRFGVRRLAAAGAMLALLATLPFVYLALR
HHCCCCCCCCEEHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYDPVVLVPALFLRGMGQSAIGAPSITAAYASVERRDLPMATTSLNIVQRLGGPTLTTLC
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
TLFLAWRLQADHVAGGDAHGTAYAWAFGLLCVLHASSFATALRLPLWSAGAGGRRAAPAS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SGSR
CCCC
>Mature Secondary Structure 
SDERTQPAPARVGGGLDPSIWKVSAVAMLGSLLSQLDATIVNVSLSSLATDLQTSLSTI
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWVTSGYLLALTLVLPLNGWLVDRIGAKALYLWCFSAFTLTSALCGLAWSAPSLIAFRAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QGMSGGLLAPMAQMMIARVAGQQMARVIGYAAVPVLLAPILGPVVAGAILQHTSWRWLFL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VNLPVGVLALALAAWFLPGDREETQPRALDWIGLSLLSPGLVLFLYGVERIGNALGIAAL
ECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAALLLVAFLRVETRMGDRALLDLALFRRKVFGAAASTQFLSNGALFAGQMLIPVFLIQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ACGRSPGEMGWLLAPMGLGMLVTYPSMGALTGRFGVRRLAAAGAMLALLATLPFVYLALR
HHCCCCCCCCEEHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYDPVVLVPALFLRGMGQSAIGAPSITAAYASVERRDLPMATTSLNIVQRLGGPTLTTLC
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
TLFLAWRLQADHVAGGDAHGTAYAWAFGLLCVLHASSFATALRLPLWSAGAGGRRAAPAS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SGSR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]