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The map label for this gene is emrD [H]

Identifier: 189353179

GI number: 189353179

Start: 1403017

End: 1404264

Strand: Reverse

Name: emrD [H]

Synonym: BMULJ_04415

Alternate gene names: 189353179

Gene position: 1404264-1403017 (Counterclockwise)

Preceding gene: 189353183

Following gene: 189353175

Centisome position: 56.78

GC content: 66.27

Gene sequence:

>1248_bases
ATGAATCCCACACTCGCTTCCGCGCGCGAGTCGAACTCGCCGCGCTTCCTGCTGTTCCTGATCTGCCTGTTCGCTTCCGC
CGGCCAGCTCGCGATCGACATCTACGTGCCCGCACTGCCCGACATGGCACGCTCGTTCGCGACGACGCCGCAGGCGATCC
AGTCGAGCGTGTCGGGCTACATGGCCGCATATGCGCTCGGTCAGCTGATCTTCGGCCCGATCGCCGACGCGTACGGCCGC
AAGCGCGTGCTCGCGTTCGGTCTCGTGATCTATACGATCGGCTGCCTGCTGTCGCTCGGCGCGCCGAACCTCGAGACCTT
CGTGCTCGCGCGCTGTCTGCAGGGTTTCGGGATCGCGACGACGAACCTGCTCGCGAAGGCGATCATCACCGATTCGTTCT
CGGGGCAGGCGCTGATGCACGCGTTCACCTACATGTCAATCGCCTGGGGGCTCGCGCCGATCATCGCGCCGGTGATCGGC
GCGCATCTGCAGACCTGGTTCGGCTGGCGCGCGTGCCTCGTGTTCCTGCTCGTCTATTCGCTCGTGATGTGGGCGCTGCT
GTGGCGCTACCGCGAGACCTTGCCGAAGCCCGTTCATCTCGAGCCGCGCACGCTGATCGCGAATGCGGGCAAGGTGCTGG
CGAGCCCGGTATTCCAGAGCTGCTTCCTCGCGCAAGGGCTGTGCTACAGCATCCTGCTCGTGTTCAACATCGTCGGGCCG
TTCATGGTGCAGAACACGCTGCACCAGCCGCCGACGTTCTTCGGCTATCTCGCGCTCGGCATCGGGCTGATGTATTTCCT
CGGCGGCCTGTCGAACCGGATTCACGGACGCGGCCTGCCGAGCGCCGAGCAGCGGCTGCGCATCGGCGCGCGCGTGATGG
CGTGCGCGGCCGTCGCGATGCTCGTGCTCGCGCTGACCGTCGGCCTGCGCGTCTGGACGCTCGCGACGCCGGTGCTCGTG
ATGGGCTTCTGCGCAGGCGCGATGTATCCGACGCTGATGGCCAAGGGCAATTCGCTGTTTCCGCATATCGCGGGGCTCAC
CAGCGCGATTCTCGGCTGTGCGCTGCTGCTCGTATCGTCCGCGATGATGGGGCTCGCCGGCTTCGTGTCGGTGCAGGTGC
TGACGCCGCTCGCGCTGTTCTTCGTCGCGCTGTCGTTCGTCGTCGTCTGGATGGTGACGAAGTTGCTGCGCTATCTGACG
CAGCAGCAGGCTGCGAGCGTCGCATGCGGGAGCGGCGAGACCGCGTAA

Upstream 100 bases:

>100_bases
GCAACAACAGGCACAATACGCACCCACGCGCCGCGCACACGTGTTCGCATTTCGAACGACGTGGCGATGCGTCGCCGCCG
TTCGAATCGTTCCCGTCGTC

Downstream 100 bases:

>100_bases
GCGCGCGCGGCGTTCACAGCGGATGGGCCGCGCCGGCCTGCGTCAGCAGCGCGACGGCCGCCGCAGCCGGCATCCCGTTC
GAGTCGATCGCGCCGGCCTT

Product: 2-module integral membrane pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNPTLASARESNSPRFLLFLICLFASAGQLAIDIYVPALPDMARSFATTPQAIQSSVSGYMAAYALGQLIFGPIADAYGR
KRVLAFGLVIYTIGCLLSLGAPNLETFVLARCLQGFGIATTNLLAKAIITDSFSGQALMHAFTYMSIAWGLAPIIAPVIG
AHLQTWFGWRACLVFLLVYSLVMWALLWRYRETLPKPVHLEPRTLIANAGKVLASPVFQSCFLAQGLCYSILLVFNIVGP
FMVQNTLHQPPTFFGYLALGIGLMYFLGGLSNRIHGRGLPSAEQRLRIGARVMACAAVAMLVLALTVGLRVWTLATPVLV
MGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSSAMMGLAGFVSVQVLTPLALFFVALSFVVVWMVTKLLRYLT
QQQAASVACGSGETA

Sequences:

>Translated_415_residues
MNPTLASARESNSPRFLLFLICLFASAGQLAIDIYVPALPDMARSFATTPQAIQSSVSGYMAAYALGQLIFGPIADAYGR
KRVLAFGLVIYTIGCLLSLGAPNLETFVLARCLQGFGIATTNLLAKAIITDSFSGQALMHAFTYMSIAWGLAPIIAPVIG
AHLQTWFGWRACLVFLLVYSLVMWALLWRYRETLPKPVHLEPRTLIANAGKVLASPVFQSCFLAQGLCYSILLVFNIVGP
FMVQNTLHQPPTFFGYLALGIGLMYFLGGLSNRIHGRGLPSAEQRLRIGARVMACAAVAMLVLALTVGLRVWTLATPVLV
MGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSSAMMGLAGFVSVQVLTPLALFFVALSFVVVWMVTKLLRYLT
QQQAASVACGSGETA
>Mature_415_residues
MNPTLASARESNSPRFLLFLICLFASAGQLAIDIYVPALPDMARSFATTPQAIQSSVSGYMAAYALGQLIFGPIADAYGR
KRVLAFGLVIYTIGCLLSLGAPNLETFVLARCLQGFGIATTNLLAKAIITDSFSGQALMHAFTYMSIAWGLAPIIAPVIG
AHLQTWFGWRACLVFLLVYSLVMWALLWRYRETLPKPVHLEPRTLIANAGKVLASPVFQSCFLAQGLCYSILLVFNIVGP
FMVQNTLHQPPTFFGYLALGIGLMYFLGGLSNRIHGRGLPSAEQRLRIGARVMACAAVAMLVLALTVGLRVWTLATPVLV
MGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSSAMMGLAGFVSVQVLTPLALFFVALSFVVVWMVTKLLRYLT
QQQAASVACGSGETA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=343, Percent_Identity=27.6967930029155, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI48994889, Length=344, Percent_Identity=27.0348837209302, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87082312, Length=339, Percent_Identity=28.023598820059, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790794, Length=409, Percent_Identity=23.4718826405868, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787065, Length=157, Percent_Identity=28.0254777070064, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790146, Length=237, Percent_Identity=25.7383966244726, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1790195, Length=136, Percent_Identity=29.4117647058824, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=236, Percent_Identity=25, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44478; Mature: 44478

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTLASARESNSPRFLLFLICLFASAGQLAIDIYVPALPDMARSFATTPQAIQSSVSGY
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHH
MAAYALGQLIFGPIADAYGRKRVLAFGLVIYTIGCLLSLGAPNLETFVLARCLQGFGIAT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
TNLLAKAIITDSFSGQALMHAFTYMSIAWGLAPIIAPVIGAHLQTWFGWRACLVFLLVYS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVMWALLWRYRETLPKPVHLEPRTLIANAGKVLASPVFQSCFLAQGLCYSILLVFNIVGP
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMVQNTLHQPPTFFGYLALGIGLMYFLGGLSNRIHGRGLPSAEQRLRIGARVMACAAVAM
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLALTVGLRVWTLATPVLVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AMMGLAGFVSVQVLTPLALFFVALSFVVVWMVTKLLRYLTQQQAASVACGSGETA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNPTLASARESNSPRFLLFLICLFASAGQLAIDIYVPALPDMARSFATTPQAIQSSVSGY
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHH
MAAYALGQLIFGPIADAYGRKRVLAFGLVIYTIGCLLSLGAPNLETFVLARCLQGFGIAT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
TNLLAKAIITDSFSGQALMHAFTYMSIAWGLAPIIAPVIGAHLQTWFGWRACLVFLLVYS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVMWALLWRYRETLPKPVHLEPRTLIANAGKVLASPVFQSCFLAQGLCYSILLVFNIVGP
HHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMVQNTLHQPPTFFGYLALGIGLMYFLGGLSNRIHGRGLPSAEQRLRIGARVMACAAVAM
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLALTVGLRVWTLATPVLVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AMMGLAGFVSVQVLTPLALFFVALSFVVVWMVTKLLRYLTQQQAASVACGSGETA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]