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The map label for this gene is ynfL [H]

Identifier: 189353146

GI number: 189353146

Start: 1344304

End: 1345212

Strand: Reverse

Name: ynfL [H]

Synonym: BMULJ_04382

Alternate gene names: 189353146

Gene position: 1345212-1344304 (Counterclockwise)

Preceding gene: 189353149

Following gene: 189353144

Centisome position: 54.39

GC content: 68.87

Gene sequence:

>909_bases
ATGATCGAACTGCACCAGCTTCGCTGCTTCGTCGCGGTCGCCGAGGAACTGCACTTCGGGCGCGCGGCGAAGCGGCTCTT
CATGACGCAGCCGCCGCTGAGCCGGCAGATCCAGTTGCTCGAACATGCGCTCGGCATCGCGTTGCTCGAGCGCAGCAGCC
GGCAGGTGAGCCTGACCGCGGCCGGCGAACGCTTTCTGCGCGACGCGCGGCACATCCTCGAATTCTCGGCGCGGGCCGAA
CAGGCCGCGCAGCGCGTCGCGCACGGCGGCGCGGGCCGCATCACGCTCGGCTTCACGGCGGTCAGCGCTTACCGGATGAT
TCCGATGCTGCTCGCGCATGCGGCGCACGCCTTGCCCGACGTCGACGTCGAGTTGCGCGAGATGGTGTCGACGGTGCAGA
TCGACGCGCTCGCGTCGCGGATGCTCGATGCGGGGTTCGTGCGCCAGCGCGCCGCGCGCCAGCCGCTCGAATACCGGCTC
GTGCAGCGCGAGCCGATGCTCGTCGCGGTTGCGGAAGGCGCGCCGCTCGCCGTACACGAACGGATCGGCCCCGACGATCT
CGATCGCCAGCCCTTCATCGCGTATTCGCCGAACGAGGGCAAGTATTTCCACGACATGATTTCCGGGATGTTCGCGAGCG
CCGGGCGGCTGCCGAACTACCTGCATTACGTCGGGCAGACGCATACGATCCTCGGCCTCGTGCGCGCGGGGCTTGGCGCG
GCGCTGGTGCCGGCGTCCGCGCGCGAACTGCACGTGGACGGCGTCGTGTTTCGTCCGCTCGCGGACGTCGACGTCGCGGC
CGAGCTGTATCTCGCGTGGCGGACCGACAACGACAATCCGGCGCTGCCGGTATTCAATGCGATGGTCGAGCGGTTTCTGA
CGGAAAGCGCGGACGGCGGCATCGCCTGA

Upstream 100 bases:

>100_bases
GGCGTGCGCGGCGCGGGCCGCGCATCGGGTGCAACCGAGTATAGGAATCGCATCGATGTTGGTCTAACATCAAACGCCTA
TTGATTGATTCAACTATTGA

Downstream 100 bases:

>100_bases
GCGTCGCGTCGGCGAGCGAGTGCGACGCGGGAAGGCTCAAAACGAAAAGGCGAGGGGAAAGGGGCGGAGCGCAGGCCTGG
CCGCACGCCGGAATCGACGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MIELHQLRCFVAVAEELHFGRAAKRLFMTQPPLSRQIQLLEHALGIALLERSSRQVSLTAAGERFLRDARHILEFSARAE
QAAQRVAHGGAGRITLGFTAVSAYRMIPMLLAHAAHALPDVDVELREMVSTVQIDALASRMLDAGFVRQRAARQPLEYRL
VQREPMLVAVAEGAPLAVHERIGPDDLDRQPFIAYSPNEGKYFHDMISGMFASAGRLPNYLHYVGQTHTILGLVRAGLGA
ALVPASARELHVDGVVFRPLADVDVAAELYLAWRTDNDNPALPVFNAMVERFLTESADGGIA

Sequences:

>Translated_302_residues
MIELHQLRCFVAVAEELHFGRAAKRLFMTQPPLSRQIQLLEHALGIALLERSSRQVSLTAAGERFLRDARHILEFSARAE
QAAQRVAHGGAGRITLGFTAVSAYRMIPMLLAHAAHALPDVDVELREMVSTVQIDALASRMLDAGFVRQRAARQPLEYRL
VQREPMLVAVAEGAPLAVHERIGPDDLDRQPFIAYSPNEGKYFHDMISGMFASAGRLPNYLHYVGQTHTILGLVRAGLGA
ALVPASARELHVDGVVFRPLADVDVAAELYLAWRTDNDNPALPVFNAMVERFLTESADGGIA
>Mature_302_residues
MIELHQLRCFVAVAEELHFGRAAKRLFMTQPPLSRQIQLLEHALGIALLERSSRQVSLTAAGERFLRDARHILEFSARAE
QAAQRVAHGGAGRITLGFTAVSAYRMIPMLLAHAAHALPDVDVELREMVSTVQIDALASRMLDAGFVRQRAARQPLEYRL
VQREPMLVAVAEGAPLAVHERIGPDDLDRQPFIAYSPNEGKYFHDMISGMFASAGRLPNYLHYVGQTHTILGLVRAGLGA
ALVPASARELHVDGVVFRPLADVDVAAELYLAWRTDNDNPALPVFNAMVERFLTESADGGIA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=273, Percent_Identity=39.9267399267399, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=33.5616438356164, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI2367136, Length=283, Percent_Identity=34.2756183745583, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI145693105, Length=320, Percent_Identity=26.25, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=29.136690647482, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=30.0813008130081, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=24.1803278688525, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=304, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=26.2975778546713, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786713, Length=89, Percent_Identity=42.6966292134831, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI145693193, Length=257, Percent_Identity=27.2373540856031, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=238, Percent_Identity=29.8319327731092, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=123, Percent_Identity=34.1463414634146, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788297, Length=175, Percent_Identity=26.8571428571429, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788626, Length=278, Percent_Identity=26.978417266187, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787530, Length=274, Percent_Identity=24.4525547445255, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33200; Mature: 33200

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELHQLRCFVAVAEELHFGRAAKRLFMTQPPLSRQIQLLEHALGIALLERSSRQVSLTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEH
AGERFLRDARHILEFSARAEQAAQRVAHGGAGRITLGFTAVSAYRMIPMLLAHAAHALPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
VDVELREMVSTVQIDALASRMLDAGFVRQRAARQPLEYRLVQREPMLVAVAEGAPLAVHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCEEEEC
RIGPDDLDRQPFIAYSPNEGKYFHDMISGMFASAGRLPNYLHYVGQTHTILGLVRAGLGA
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCC
ALVPASARELHVDGVVFRPLADVDVAAELYLAWRTDNDNPALPVFNAMVERFLTESADGG
CCCCCCCCEEEECCEEECCCCCHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
IA
CC
>Mature Secondary Structure
MIELHQLRCFVAVAEELHFGRAAKRLFMTQPPLSRQIQLLEHALGIALLERSSRQVSLTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEH
AGERFLRDARHILEFSARAEQAAQRVAHGGAGRITLGFTAVSAYRMIPMLLAHAAHALPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
VDVELREMVSTVQIDALASRMLDAGFVRQRAARQPLEYRLVQREPMLVAVAEGAPLAVHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCEEEEC
RIGPDDLDRQPFIAYSPNEGKYFHDMISGMFASAGRLPNYLHYVGQTHTILGLVRAGLGA
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCC
ALVPASARELHVDGVVFRPLADVDVAAELYLAWRTDNDNPALPVFNAMVERFLTESADGG
CCCCCCCCEEEECCEEECCCCCHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]