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The map label for this gene is nirD [H]

Identifier: 189353121

GI number: 189353121

Start: 1311997

End: 1312341

Strand: Reverse

Name: nirD [H]

Synonym: BMULJ_04357

Alternate gene names: 189353121

Gene position: 1312341-1311997 (Counterclockwise)

Preceding gene: 189353122

Following gene: 189353120

Centisome position: 53.06

GC content: 68.7

Gene sequence:

>345_bases
ATGAACGATCGTCTTCCGCAGTCCTGGACGCGCGTCTGCGCACTCGACGACATCGTGCCGAACACCGGCGTCTGCGCACT
CGTCAACGGCGAGCAGGTCGCCGTCTTCCACGTCGCGCACGGCGACGGCGGCGTGTACGCCATCGACAACGTCGATCCGG
TCTCGCGCGCGGCCGTGATGTCGCGCGGGCTGATCGGCAGCCTCGGCGAGCGCGTGGTGGTCGCCTCGCCGCTCTACAAG
CACCACTTCGACCTGCGCACCGGCGAATGCGTCGAGGCGCCCGAGCAATCGGTCAATGCGTATCCGTCGCGCGTCGAGGA
CGGCTACGTGTGGGTCGCGGCCTGA

Upstream 100 bases:

>100_bases
TCGGCGGCAAGCGCGTCGCGATCCCGGTCGTCGCGGCGGAGCGCGTCGCCGAATCCGAAACCGTCTGATCCGTGCCACCG
TCATCTTCAAGGAGAGTCCG

Downstream 100 bases:

>100_bases
CCATCGCGGAGCGCGCATGACCGCCACCAGTGTGAAGAGCGTGTGCCCGTACTGCGGCGTCGGCTGCGGGATGGTGCTGC
ACGTCGAGGACGGCGAGGTC

Product: NAD(P)H-dependent nitrite reductase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MNDRLPQSWTRVCALDDIVPNTGVCALVNGEQVAVFHVAHGDGGVYAIDNVDPVSRAAVMSRGLIGSLGERVVVASPLYK
HHFDLRTGECVEAPEQSVNAYPSRVEDGYVWVAA

Sequences:

>Translated_114_residues
MNDRLPQSWTRVCALDDIVPNTGVCALVNGEQVAVFHVAHGDGGVYAIDNVDPVSRAAVMSRGLIGSLGERVVVASPLYK
HHFDLRTGECVEAPEQSVNAYPSRVEDGYVWVAA
>Mature_114_residues
MNDRLPQSWTRVCALDDIVPNTGVCALVNGEQVAVFHVAHGDGGVYAIDNVDPVSRAAVMSRGLIGSLGERVVVASPLYK
HHFDLRTGECVEAPEQSVNAYPSRVEDGYVWVAA

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=103, Percent_Identity=48.5436893203884, Blast_Score=100, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 12264; Mature: 12264

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRLPQSWTRVCALDDIVPNTGVCALVNGEQVAVFHVAHGDGGVYAIDNVDPVSRAAVM
CCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEECCCCEEEECCCCHHHHHHHH
SRGLIGSLGERVVVASPLYKHHFDLRTGECVEAPEQSVNAYPSRVEDGYVWVAA
HCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNDRLPQSWTRVCALDDIVPNTGVCALVNGEQVAVFHVAHGDGGVYAIDNVDPVSRAAVM
CCCCCCCHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEECCCCEEEECCCCHHHHHHHH
SRGLIGSLGERVVVASPLYKHHFDLRTGECVEAPEQSVNAYPSRVEDGYVWVAA
HCCCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]